TY  - JOUR
AU  - Župunski, Vesna
AU  - Vasić, Mirjana
AU  - Sustar-Vozlić, Jelka
AU  - Maras, Marko
AU  - Savić, Aleksandra
AU  - Petrović, Gordana
AU  - Živanov, Dalibor
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1804
AB  - Trypsin inhibitors could limit utilization of legumes in human nutrition, but they could also have beneficial health effects. The objective of this study was to measure trypsin inhibitor activity (TIA) of different legumes using microtiter plate method and to identify factors that contribute to uncertainty of TIA measurement. TIA measurements were performed on seeds of faba bean, pea, common vetch, soybean, and common bean cultivars. The significant effect of legume crop on TIA measurement uncertainty was confirmed with P = 0.045. Certain sources of measurement uncertainty were related with the content of trypsin inhibitors (Tis) in legume seeds. In respect to that, significant effect of level of sample dilution (P E, 0.001) was confirmed. Significant influence of the repeated absorbance measurement of sample reaction mixture on uncertainty of TIA measurement was identified (P E, 0.001), and it took 60% of overall TIA measurement uncertainty for soybean cultivars. TIA of soybean cultivars exceeded 90 TUI/mg. Repeated absorbance measurement of positive control reaction mixture took 70% of TIA measurement uncertainty of cultivars with TIA lesser than 4.5 TUI/mg. Graduated cylinder used for preparation of the final sample solutions took the range from 45 to 90% of overall TIA measurement uncertainty of the cultivars whose TIA were in the middle of previously mentioned. The uncertainty of TIA measurement of legume crops was not studied before; thus, this study pointed out that acquiring insight into factors contributing to uncertainty of TIA measurement could give directions for improvement of TIA testing if microtiter plate method is used.
PB  - Springer, New York
T2  - Food Analytical Methods
T1  - Uncertainty of Trypsin Inhibitor Activity Measurement of Legume Crops Using Microtiter Plate Method
EP  - 1040
IS  - 4
SP  - 1034
VL  - 11
DO  - 10.1007/s12161-017-1076-y
ER  - 

@article{
author = "Župunski, Vesna and Vasić, Mirjana and Sustar-Vozlić, Jelka and Maras, Marko and Savić, Aleksandra and Petrović, Gordana and Živanov, Dalibor",
year = "2018",
abstract = "Trypsin inhibitors could limit utilization of legumes in human nutrition, but they could also have beneficial health effects. The objective of this study was to measure trypsin inhibitor activity (TIA) of different legumes using microtiter plate method and to identify factors that contribute to uncertainty of TIA measurement. TIA measurements were performed on seeds of faba bean, pea, common vetch, soybean, and common bean cultivars. The significant effect of legume crop on TIA measurement uncertainty was confirmed with P = 0.045. Certain sources of measurement uncertainty were related with the content of trypsin inhibitors (Tis) in legume seeds. In respect to that, significant effect of level of sample dilution (P E, 0.001) was confirmed. Significant influence of the repeated absorbance measurement of sample reaction mixture on uncertainty of TIA measurement was identified (P E, 0.001), and it took 60% of overall TIA measurement uncertainty for soybean cultivars. TIA of soybean cultivars exceeded 90 TUI/mg. Repeated absorbance measurement of positive control reaction mixture took 70% of TIA measurement uncertainty of cultivars with TIA lesser than 4.5 TUI/mg. Graduated cylinder used for preparation of the final sample solutions took the range from 45 to 90% of overall TIA measurement uncertainty of the cultivars whose TIA were in the middle of previously mentioned. The uncertainty of TIA measurement of legume crops was not studied before; thus, this study pointed out that acquiring insight into factors contributing to uncertainty of TIA measurement could give directions for improvement of TIA testing if microtiter plate method is used.",
publisher = "Springer, New York",
journal = "Food Analytical Methods",
title = "Uncertainty of Trypsin Inhibitor Activity Measurement of Legume Crops Using Microtiter Plate Method",
pages = "1040-1034",
number = "4",
volume = "11",
doi = "10.1007/s12161-017-1076-y"
}

Župunski, V., Vasić, M., Sustar-Vozlić, J., Maras, M., Savić, A., Petrović, G.,& Živanov, D.. (2018). Uncertainty of Trypsin Inhibitor Activity Measurement of Legume Crops Using Microtiter Plate Method. in Food Analytical Methods
Springer, New York., 11(4), 1034-1040.
https://doi.org/10.1007/s12161-017-1076-y

Župunski V, Vasić M, Sustar-Vozlić J, Maras M, Savić A, Petrović G, Živanov D. Uncertainty of Trypsin Inhibitor Activity Measurement of Legume Crops Using Microtiter Plate Method. in Food Analytical Methods. 2018;11(4):1034-1040.
doi:10.1007/s12161-017-1076-y .

Župunski, Vesna, Vasić, Mirjana, Sustar-Vozlić, Jelka, Maras, Marko, Savić, Aleksandra, Petrović, Gordana, Živanov, Dalibor, "Uncertainty of Trypsin Inhibitor Activity Measurement of Legume Crops Using Microtiter Plate Method" in Food Analytical Methods, 11, no. 4 (2018):1034-1040,
https://doi.org/10.1007/s12161-017-1076-y . .

TY  - JOUR
AU  - Maras, Marko
AU  - Pipan, Barbara
AU  - Sustar-Vozlić, Jelka
AU  - Todorović, Vida
AU  - Durić, Gordana
AU  - Vasić, Mirjana
AU  - Kratovalieva, Suzana
AU  - Ibusoska, Afrodita
AU  - Agić, Rukie
AU  - Matotan, Zdravko
AU  - Cupić, Tihomir
AU  - Meglić, Vladimir
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1421
AB  - In this study, genetic diversity of 119 accessions of common bean (Phaseolus vulgaris) from five former Yugoslav republics constituting the western Balkans was assessed by 13 microsatellite markers. This set of markers has proven before to efficiently distinguish between bean genotypes and assign them to either the Andean or the Mesoamerican gene pool of origin. In this study, 118 alleles were detected or 9.1 per locus on average. Four groups (i.e., Slovene, Croatian, Bosnian, and Serbian) showed similarly high levels of genetic diversity as estimated by the number of different alleles, number of effective alleles, Shannon's information index, and expected heterozygosity. Mildly narrower genetic diversity was identified within a group of Macedonian accessions; however, this germplasm yielded the highest number of private alleles. All five germplasms share a great portion of genetic diversity as indicated by the analysis of molecular variance (AMOVA). On the basis of the scored number of migrants, we concluded that the most intensive gene flow in the region exists in Bosnia and Herzegovina. Cluster analysis based on collected molecular data classified the accessions into two large clusters that corresponded to two gene pools of origin (i.e., Andean and Mesoamerican). We found that Andean genotypes are more prevalent than Mesoamerican in all studied countries, except Macedonia, where the two gene pools are represented evenly. This could indicate that common bean was introduced into the western Balkans mainly from the Mediterranean Basin. Bayesian cluster analysis revealed that in the area studied an additional variation exists which is related to the Andean gene pool. Different scenarios of the origin of this variation are discussed in the article.
PB  - Amer Soc Horticultural Science, Alexandria
T2  - Journal of The American Society for Horticultural Science
T1  - Examination of Genetic Diversity of Common Bean from the Western Balkans
EP  - 316
IS  - 4
SP  - 308
VL  - 140
DO  - 10.21273/JASHS.140.4.308
ER  - 

@article{
author = "Maras, Marko and Pipan, Barbara and Sustar-Vozlić, Jelka and Todorović, Vida and Durić, Gordana and Vasić, Mirjana and Kratovalieva, Suzana and Ibusoska, Afrodita and Agić, Rukie and Matotan, Zdravko and Cupić, Tihomir and Meglić, Vladimir",
year = "2015",
abstract = "In this study, genetic diversity of 119 accessions of common bean (Phaseolus vulgaris) from five former Yugoslav republics constituting the western Balkans was assessed by 13 microsatellite markers. This set of markers has proven before to efficiently distinguish between bean genotypes and assign them to either the Andean or the Mesoamerican gene pool of origin. In this study, 118 alleles were detected or 9.1 per locus on average. Four groups (i.e., Slovene, Croatian, Bosnian, and Serbian) showed similarly high levels of genetic diversity as estimated by the number of different alleles, number of effective alleles, Shannon's information index, and expected heterozygosity. Mildly narrower genetic diversity was identified within a group of Macedonian accessions; however, this germplasm yielded the highest number of private alleles. All five germplasms share a great portion of genetic diversity as indicated by the analysis of molecular variance (AMOVA). On the basis of the scored number of migrants, we concluded that the most intensive gene flow in the region exists in Bosnia and Herzegovina. Cluster analysis based on collected molecular data classified the accessions into two large clusters that corresponded to two gene pools of origin (i.e., Andean and Mesoamerican). We found that Andean genotypes are more prevalent than Mesoamerican in all studied countries, except Macedonia, where the two gene pools are represented evenly. This could indicate that common bean was introduced into the western Balkans mainly from the Mediterranean Basin. Bayesian cluster analysis revealed that in the area studied an additional variation exists which is related to the Andean gene pool. Different scenarios of the origin of this variation are discussed in the article.",
publisher = "Amer Soc Horticultural Science, Alexandria",
journal = "Journal of The American Society for Horticultural Science",
title = "Examination of Genetic Diversity of Common Bean from the Western Balkans",
pages = "316-308",
number = "4",
volume = "140",
doi = "10.21273/JASHS.140.4.308"
}

Maras, M., Pipan, B., Sustar-Vozlić, J., Todorović, V., Durić, G., Vasić, M., Kratovalieva, S., Ibusoska, A., Agić, R., Matotan, Z., Cupić, T.,& Meglić, V.. (2015). Examination of Genetic Diversity of Common Bean from the Western Balkans. in Journal of The American Society for Horticultural Science
Amer Soc Horticultural Science, Alexandria., 140(4), 308-316.
https://doi.org/10.21273/JASHS.140.4.308

Maras M, Pipan B, Sustar-Vozlić J, Todorović V, Durić G, Vasić M, Kratovalieva S, Ibusoska A, Agić R, Matotan Z, Cupić T, Meglić V. Examination of Genetic Diversity of Common Bean from the Western Balkans. in Journal of The American Society for Horticultural Science. 2015;140(4):308-316.
doi:10.21273/JASHS.140.4.308 .

Maras, Marko, Pipan, Barbara, Sustar-Vozlić, Jelka, Todorović, Vida, Durić, Gordana, Vasić, Mirjana, Kratovalieva, Suzana, Ibusoska, Afrodita, Agić, Rukie, Matotan, Zdravko, Cupić, Tihomir, Meglić, Vladimir, "Examination of Genetic Diversity of Common Bean from the Western Balkans" in Journal of The American Society for Horticultural Science, 140, no. 4 (2015):308-316,
https://doi.org/10.21273/JASHS.140.4.308 . .

TY  - CONF
AU  - Meglič, Vladimir
AU  - Pipan, Barbara
AU  - Šuštar-Vozlič, Jelka
AU  - Maras, Marko
AU  - Todorović, Vida
AU  - Vasić, Mirjana
AU  - Kratovalieva, Suzana
AU  - Ibusoska, Afrodita
AU  - Matotan, Zdravko
AU  - Čupić, Tihomir
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/2984
AB  - Genetic differentiation of 167 Phaseolus vulgaris L. accessions originating from five South east European countries was performed using 13 SSR markers. Analysis included 21 accessions from Bosnia and Herzegovina, 15 accessions from Croatia, 73 accessions from Macedonia, 38 accessions from Serbia and 20 accessions from Slovenia. Calculated mean number of alleles per locus was 9.8. The average polymorphic information content over all loci reached value of 0.710 where the most informative locus was GATS91 (0.926). These results showed that selected set of SSR markers are highly informative and applicable for studies of genetic diversity within germplasm collected in the five South east European countries, which was also confirmed with the calculated value of probability of identity (1.831x10). High mean value of expected heterozygosity (0.731) and Shannon’s information index (1.486) reflects high genetic diversity within accessions from five countries, where accessions from Macedonia are genetically the most uniform, due to 17completely identical accessions included.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Genetic diversity of the autochtonous Phaseolus bean germplasm originating from five South east European countries
EP  - 29
SP  - 29
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2984
ER  - 

@conference{
author = "Meglič, Vladimir and Pipan, Barbara and Šuštar-Vozlič, Jelka and Maras, Marko and Todorović, Vida and Vasić, Mirjana and Kratovalieva, Suzana and Ibusoska, Afrodita and Matotan, Zdravko and Čupić, Tihomir",
year = "2013",
abstract = "Genetic differentiation of 167 Phaseolus vulgaris L. accessions originating from five South east European countries was performed using 13 SSR markers. Analysis included 21 accessions from Bosnia and Herzegovina, 15 accessions from Croatia, 73 accessions from Macedonia, 38 accessions from Serbia and 20 accessions from Slovenia. Calculated mean number of alleles per locus was 9.8. The average polymorphic information content over all loci reached value of 0.710 where the most informative locus was GATS91 (0.926). These results showed that selected set of SSR markers are highly informative and applicable for studies of genetic diversity within germplasm collected in the five South east European countries, which was also confirmed with the calculated value of probability of identity (1.831x10). High mean value of expected heterozygosity (0.731) and Shannon’s information index (1.486) reflects high genetic diversity within accessions from five countries, where accessions from Macedonia are genetically the most uniform, due to 17completely identical accessions included.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Genetic diversity of the autochtonous Phaseolus bean germplasm originating from five South east European countries",
pages = "29-29",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2984"
}

Meglič, V., Pipan, B., Šuštar-Vozlič, J., Maras, M., Todorović, V., Vasić, M., Kratovalieva, S., Ibusoska, A., Matotan, Z.,& Čupić, T.. (2013). Genetic diversity of the autochtonous Phaseolus bean germplasm originating from five South east European countries. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 29-29.
https://hdl.handle.net/21.15107/rcub_fiver_2984

Meglič V, Pipan B, Šuštar-Vozlič J, Maras M, Todorović V, Vasić M, Kratovalieva S, Ibusoska A, Matotan Z, Čupić T. Genetic diversity of the autochtonous Phaseolus bean germplasm originating from five South east European countries. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:29-29.
https://hdl.handle.net/21.15107/rcub_fiver_2984 .

Meglič, Vladimir, Pipan, Barbara, Šuštar-Vozlič, Jelka, Maras, Marko, Todorović, Vida, Vasić, Mirjana, Kratovalieva, Suzana, Ibusoska, Afrodita, Matotan, Zdravko, Čupić, Tihomir, "Genetic diversity of the autochtonous Phaseolus bean germplasm originating from five South east European countries" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):29-29,
https://hdl.handle.net/21.15107/rcub_fiver_2984 .