TY  - JOUR
AU  - Radanović, Aleksandra
AU  - Sprycha, Yves
AU  - Jocković, Milan
AU  - Sundt, Monja
AU  - Miladinović, Dragana
AU  - Jansen, Constantin
AU  - Horn, Renate
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2655
AB  - Single nucleotide polymorphisms (SNPs) were significantly associated with fertility restoration of cytoplasmic male sterility (CMS) PET1 by the restorer gene Rf1. For these SNPs, four Kompetitive allele-specific PCR (KASP) markers were successfully designed. The KASP markers cover the fertility restorer locus Rf1, spanning about 3 Mb, and clearly differentiate restorer and maintainer lines. For genetic purity testing in sunflower hybrid production, the efficiency for detecting contaminations in samples was simulated using mixtures of hypocotyls or leaves. Contaminations of restorer lines with 1%, 3%, 5%, 10%, and 50% of maintainer lines were screened with all four KASP markers. Contaminations of 10% could be clearly detected in pools of 100 plants. Contaminations below this level require detection on a single plant level. For single plant detections, ethyl methanesulfonate-treated sunflower F1 hybrids, which had been phenotypically evaluated for male sterility (potential mutation in the Rf1 gene) were screened. Nine identified either partially male-sterile or male-sterile plants were analyzed with all four KASP markers and only one proved to be a hybrid with a mutation, seven were male-sterile contaminants in the F1 seeds used (1.6%) and one a recombinant plant. The four KASP markers should be valuable tools for marker-assisted selection (MAS) in sunflower breeding regarding the restorer locus Rf1.
PB  - Basel : MDPI
T2  - Genes
T1  - KASP markers specific for the fertility restorer locus Rf1 and application for genetic purity testing in sunflowers (Helianthus annuus L.)
SP  - 465
VL  - 13
DO  - 10.3390/genes13030465
ER  - 

@article{
author = "Radanović, Aleksandra and Sprycha, Yves and Jocković, Milan and Sundt, Monja and Miladinović, Dragana and Jansen, Constantin and Horn, Renate",
year = "2022",
abstract = "Single nucleotide polymorphisms (SNPs) were significantly associated with fertility restoration of cytoplasmic male sterility (CMS) PET1 by the restorer gene Rf1. For these SNPs, four Kompetitive allele-specific PCR (KASP) markers were successfully designed. The KASP markers cover the fertility restorer locus Rf1, spanning about 3 Mb, and clearly differentiate restorer and maintainer lines. For genetic purity testing in sunflower hybrid production, the efficiency for detecting contaminations in samples was simulated using mixtures of hypocotyls or leaves. Contaminations of restorer lines with 1%, 3%, 5%, 10%, and 50% of maintainer lines were screened with all four KASP markers. Contaminations of 10% could be clearly detected in pools of 100 plants. Contaminations below this level require detection on a single plant level. For single plant detections, ethyl methanesulfonate-treated sunflower F1 hybrids, which had been phenotypically evaluated for male sterility (potential mutation in the Rf1 gene) were screened. Nine identified either partially male-sterile or male-sterile plants were analyzed with all four KASP markers and only one proved to be a hybrid with a mutation, seven were male-sterile contaminants in the F1 seeds used (1.6%) and one a recombinant plant. The four KASP markers should be valuable tools for marker-assisted selection (MAS) in sunflower breeding regarding the restorer locus Rf1.",
publisher = "Basel : MDPI",
journal = "Genes",
title = "KASP markers specific for the fertility restorer locus Rf1 and application for genetic purity testing in sunflowers (Helianthus annuus L.)",
pages = "465",
volume = "13",
doi = "10.3390/genes13030465"
}

Radanović, A., Sprycha, Y., Jocković, M., Sundt, M., Miladinović, D., Jansen, C.,& Horn, R.. (2022). KASP markers specific for the fertility restorer locus Rf1 and application for genetic purity testing in sunflowers (Helianthus annuus L.). in Genes
Basel : MDPI., 13, 465.
https://doi.org/10.3390/genes13030465

Radanović A, Sprycha Y, Jocković M, Sundt M, Miladinović D, Jansen C, Horn R. KASP markers specific for the fertility restorer locus Rf1 and application for genetic purity testing in sunflowers (Helianthus annuus L.). in Genes. 2022;13:465.
doi:10.3390/genes13030465 .

Radanović, Aleksandra, Sprycha, Yves, Jocković, Milan, Sundt, Monja, Miladinović, Dragana, Jansen, Constantin, Horn, Renate, "KASP markers specific for the fertility restorer locus Rf1 and application for genetic purity testing in sunflowers (Helianthus annuus L.)" in Genes, 13 (2022):465,
https://doi.org/10.3390/genes13030465 . .

TY  - CONF
AU  - Horn, Renate
AU  - Radanović, Aleksandra
AU  - Fuhrmann, Lena
AU  - Sprycha, Yves
AU  - Hamrit, Sonia
AU  - Jocković, Milan
AU  - Miladinović, Dragana
AU  - Jansen, Constantin
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2896
AB  - Fertility restoration is an essential part of hybrid breeding based on cytoplasmic male sterility. In sunflower, the CMS PET1 originating from an interspecific hybridization between Helianthus petiolaris and H. annuus is world-wide used in hybrid breeding. The restorer gene Rf1 is responsible for restoring fertility in the hybrids by specifically reducing the co-transcript of atp1 and the CMS-specific orfH522 in the anthers. From the mode of action (differences in RNA stability) Rf1 could be a pentatricopeptide repeat gene. Blasting BAC-end sequences and sequences of markers linked to the restorer gene Rf1 two physical locations (encompassing 30 Mb and 3.9 Mb, respectively) on linkage group 13 could be identified in the sunflower genome sequence of HanXRQ. These two regions contained nine possible candidate genes for Rf1: seven pentatricopeptide repeat genes, one aldehyde dehydrogenase and one poly(A) polymerase 3 gene. Using a next generation sequencing approach, the nine candidate genes were sequenced with 120 x coverage in an association panel of 59 lines (27 maintainer and 32 restorer lines) and 210 single nucleotide polymorphisms (SNPs) as well as 67 insertions/deletions (InDels) could be identified.
PB  - Paris : International Sunflower Association
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia
T1  - Association studies and marker development for the fertility restorer gene Rf1 in sunflower
EP  - 104
SP  - 104
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2896
ER  - 

@conference{
author = "Horn, Renate and Radanović, Aleksandra and Fuhrmann, Lena and Sprycha, Yves and Hamrit, Sonia and Jocković, Milan and Miladinović, Dragana and Jansen, Constantin",
year = "2022",
abstract = "Fertility restoration is an essential part of hybrid breeding based on cytoplasmic male sterility. In sunflower, the CMS PET1 originating from an interspecific hybridization between Helianthus petiolaris and H. annuus is world-wide used in hybrid breeding. The restorer gene Rf1 is responsible for restoring fertility in the hybrids by specifically reducing the co-transcript of atp1 and the CMS-specific orfH522 in the anthers. From the mode of action (differences in RNA stability) Rf1 could be a pentatricopeptide repeat gene. Blasting BAC-end sequences and sequences of markers linked to the restorer gene Rf1 two physical locations (encompassing 30 Mb and 3.9 Mb, respectively) on linkage group 13 could be identified in the sunflower genome sequence of HanXRQ. These two regions contained nine possible candidate genes for Rf1: seven pentatricopeptide repeat genes, one aldehyde dehydrogenase and one poly(A) polymerase 3 gene. Using a next generation sequencing approach, the nine candidate genes were sequenced with 120 x coverage in an association panel of 59 lines (27 maintainer and 32 restorer lines) and 210 single nucleotide polymorphisms (SNPs) as well as 67 insertions/deletions (InDels) could be identified.",
publisher = "Paris : International Sunflower Association, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia",
title = "Association studies and marker development for the fertility restorer gene Rf1 in sunflower",
pages = "104-104",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2896"
}

Horn, R., Radanović, A., Fuhrmann, L., Sprycha, Y., Hamrit, S., Jocković, M., Miladinović, D.,& Jansen, C.. (2022). Association studies and marker development for the fertility restorer gene Rf1 in sunflower. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia
Paris : International Sunflower Association., 104-104.
https://hdl.handle.net/21.15107/rcub_fiver_2896

Horn R, Radanović A, Fuhrmann L, Sprycha Y, Hamrit S, Jocković M, Miladinović D, Jansen C. Association studies and marker development for the fertility restorer gene Rf1 in sunflower. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia. 2022;:104-104.
https://hdl.handle.net/21.15107/rcub_fiver_2896 .

Horn, Renate, Radanović, Aleksandra, Fuhrmann, Lena, Sprycha, Yves, Hamrit, Sonia, Jocković, Milan, Miladinović, Dragana, Jansen, Constantin, "Association studies and marker development for the fertility restorer gene Rf1 in sunflower" in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia (2022):104-104,
https://hdl.handle.net/21.15107/rcub_fiver_2896 .

TY  - CONF
AU  - Radanović, Aleksandra
AU  - Horn, Renate
AU  - Sprycha, Yves
AU  - Hamrit, Sonia
AU  - Jocković, Milan
AU  - Cvejić, Sandra
AU  - Jocić, Siniša
AU  - Miklič, Vladimir
AU  - Miladinović, Dragana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/3063
AB  - Sunflower is the second most common crop among hybrids, worldwide. Development of cytoplasmic male sterile and fertility restoration lines enables creation of hybrids. Sunflower hybrid breeding most frequently relies on the combination of the CMS PET1 cytoplasm and the fertility restoration gene Rf1. Use of molecular markers can accelerate creation of restorer lines. Previous work on this subject included mapping of the Rf1 gene on chromosome 13 and development of bacterial artificial chromosome (BAC) libraries for the restorer line RHA325 and the maintainer line HA383, which enabled positioning of BAC clones surrounding the Rf1 gene in the cross RHA325 x HA342. In this study, BAC-end sequences were used to derive primers in order to amplify selected regions from RHA325 and the maintainer line, HA342. While the majority of primer combinations were monomorphic, some were polymorphic between RHA325 and HA342. Previously reported markers for detection of Rf1 gene and newly developed ones based on BAC-end sequences were further tested on hybrids and its components (A, B and R lines) created at the Institute of Field and Vegetable Crops, Novi Sad.
PB  - Belgrade : Serbian Genetic Society
C3  - Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja
T1  - Molecular markers for detection of Rf1 gene developed from BAC-end sequences in sunflower
EP  - 186
SP  - 186
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3063
ER  - 

@conference{
author = "Radanović, Aleksandra and Horn, Renate and Sprycha, Yves and Hamrit, Sonia and Jocković, Milan and Cvejić, Sandra and Jocić, Siniša and Miklič, Vladimir and Miladinović, Dragana",
year = "2019",
abstract = "Sunflower is the second most common crop among hybrids, worldwide. Development of cytoplasmic male sterile and fertility restoration lines enables creation of hybrids. Sunflower hybrid breeding most frequently relies on the combination of the CMS PET1 cytoplasm and the fertility restoration gene Rf1. Use of molecular markers can accelerate creation of restorer lines. Previous work on this subject included mapping of the Rf1 gene on chromosome 13 and development of bacterial artificial chromosome (BAC) libraries for the restorer line RHA325 and the maintainer line HA383, which enabled positioning of BAC clones surrounding the Rf1 gene in the cross RHA325 x HA342. In this study, BAC-end sequences were used to derive primers in order to amplify selected regions from RHA325 and the maintainer line, HA342. While the majority of primer combinations were monomorphic, some were polymorphic between RHA325 and HA342. Previously reported markers for detection of Rf1 gene and newly developed ones based on BAC-end sequences were further tested on hybrids and its components (A, B and R lines) created at the Institute of Field and Vegetable Crops, Novi Sad.",
publisher = "Belgrade : Serbian Genetic Society",
journal = "Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja",
title = "Molecular markers for detection of Rf1 gene developed from BAC-end sequences in sunflower",
pages = "186-186",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3063"
}

Radanović, A., Horn, R., Sprycha, Y., Hamrit, S., Jocković, M., Cvejić, S., Jocić, S., Miklič, V.,& Miladinović, D.. (2019). Molecular markers for detection of Rf1 gene developed from BAC-end sequences in sunflower. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja
Belgrade : Serbian Genetic Society., 186-186.
https://hdl.handle.net/21.15107/rcub_fiver_3063

Radanović A, Horn R, Sprycha Y, Hamrit S, Jocković M, Cvejić S, Jocić S, Miklič V, Miladinović D. Molecular markers for detection of Rf1 gene developed from BAC-end sequences in sunflower. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja. 2019;:186-186.
https://hdl.handle.net/21.15107/rcub_fiver_3063 .

Radanović, Aleksandra, Horn, Renate, Sprycha, Yves, Hamrit, Sonia, Jocković, Milan, Cvejić, Sandra, Jocić, Siniša, Miklič, Vladimir, Miladinović, Dragana, "Molecular markers for detection of Rf1 gene developed from BAC-end sequences in sunflower" in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja (2019):186-186,
https://hdl.handle.net/21.15107/rcub_fiver_3063 .

TY  - JOUR
AU  - Horn, Renate
AU  - Radanović, Aleksandra
AU  - Fuhrmann, Lena
AU  - Sprycha, Yves
AU  - Hamrit, Sonia
AU  - Jocković, Milan
AU  - Miladinović, Dragana
AU  - Jansen, Constantin
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1927
AB  - Hybrid breeding in sunflowers based on CMS PET1 requires development of restorer lines carrying, in most cases, the restorer gene Rf1. Markers for marker-assisted selection have been developed, but there is still need for closer, more versatile, and co-dominant markers linked to Rf1. Homology searches against the reference sunflower genome using sequences of cloned markers, as well as Bacterial Artificial Chromosome (BAC)-end sequences of clones hybridizing to them, allowed the identification of two genomic regions of 30 and 3.9 Mb, respectively, as possible physical locations of the restorer gene Rf1 on linkage group 13. Nine potential candidate genes, encoding six pentatricopeptide repeat proteins, one tetratricopeptide-like helical domain, a probable aldehyde dehydrogenase 22A1, and a probable poly(A) polymerase 3 (PAPS3), were identified in these two genomic regions. Amplicon targeted next generation sequencing of these nine candidate genes for Rf1 was performed in an association panel consisting of 27 maintainer and 32 restorer lines and revealed the presence of 210 Single Nucleotide Polymorphisms (SNPs) and 67 Insertions/Deletions (INDELs). Association studies showed significant associations of 10 SNPs with fertility restoration (p-value  lt  10(-4)), narrowing Rf1 down to three candidate genes. Three new markers, one co-dominant marker 67N04_P and two dominant markers, PPR621.5R for restorer, and PPR621.5M for maintainer lines were developed and verified in the association panel of 59 sunflower lines. The versatility of the three newly developed markers, as well as of three existing markers for the restorer gene Rf1 (HRG01 and HRG02, Cleaved Amplified Polymorphic Sequence (CAPS)-marker H13), was analyzed in a large association panel consisting of 557 accessions.
PB  - Basel : MDPI
T2  - International Journal of Molecular Sciences
T1  - Development and Validation of Markers for the Fertility Restorer Gene Rf1 in Sunflower
IS  - 6
VL  - 20
DO  - 10.3390/ijms20061260
ER  - 

@article{
author = "Horn, Renate and Radanović, Aleksandra and Fuhrmann, Lena and Sprycha, Yves and Hamrit, Sonia and Jocković, Milan and Miladinović, Dragana and Jansen, Constantin",
year = "2019",
abstract = "Hybrid breeding in sunflowers based on CMS PET1 requires development of restorer lines carrying, in most cases, the restorer gene Rf1. Markers for marker-assisted selection have been developed, but there is still need for closer, more versatile, and co-dominant markers linked to Rf1. Homology searches against the reference sunflower genome using sequences of cloned markers, as well as Bacterial Artificial Chromosome (BAC)-end sequences of clones hybridizing to them, allowed the identification of two genomic regions of 30 and 3.9 Mb, respectively, as possible physical locations of the restorer gene Rf1 on linkage group 13. Nine potential candidate genes, encoding six pentatricopeptide repeat proteins, one tetratricopeptide-like helical domain, a probable aldehyde dehydrogenase 22A1, and a probable poly(A) polymerase 3 (PAPS3), were identified in these two genomic regions. Amplicon targeted next generation sequencing of these nine candidate genes for Rf1 was performed in an association panel consisting of 27 maintainer and 32 restorer lines and revealed the presence of 210 Single Nucleotide Polymorphisms (SNPs) and 67 Insertions/Deletions (INDELs). Association studies showed significant associations of 10 SNPs with fertility restoration (p-value  lt  10(-4)), narrowing Rf1 down to three candidate genes. Three new markers, one co-dominant marker 67N04_P and two dominant markers, PPR621.5R for restorer, and PPR621.5M for maintainer lines were developed and verified in the association panel of 59 sunflower lines. The versatility of the three newly developed markers, as well as of three existing markers for the restorer gene Rf1 (HRG01 and HRG02, Cleaved Amplified Polymorphic Sequence (CAPS)-marker H13), was analyzed in a large association panel consisting of 557 accessions.",
publisher = "Basel : MDPI",
journal = "International Journal of Molecular Sciences",
title = "Development and Validation of Markers for the Fertility Restorer Gene Rf1 in Sunflower",
number = "6",
volume = "20",
doi = "10.3390/ijms20061260"
}

Horn, R., Radanović, A., Fuhrmann, L., Sprycha, Y., Hamrit, S., Jocković, M., Miladinović, D.,& Jansen, C.. (2019). Development and Validation of Markers for the Fertility Restorer Gene Rf1 in Sunflower. in International Journal of Molecular Sciences
Basel : MDPI., 20(6).
https://doi.org/10.3390/ijms20061260

Horn R, Radanović A, Fuhrmann L, Sprycha Y, Hamrit S, Jocković M, Miladinović D, Jansen C. Development and Validation of Markers for the Fertility Restorer Gene Rf1 in Sunflower. in International Journal of Molecular Sciences. 2019;20(6).
doi:10.3390/ijms20061260 .

Horn, Renate, Radanović, Aleksandra, Fuhrmann, Lena, Sprycha, Yves, Hamrit, Sonia, Jocković, Milan, Miladinović, Dragana, Jansen, Constantin, "Development and Validation of Markers for the Fertility Restorer Gene Rf1 in Sunflower" in International Journal of Molecular Sciences, 20, no. 6 (2019),
https://doi.org/10.3390/ijms20061260 . .

TY  - JOUR
AU  - Dimitrijević, Aleksandra
AU  - Horn, Renate
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1783
AB  - In sunflower, molecular markers for simple traits as, e.g., fertility restoration, high oleic acid content, herbicide tolerance or resistances to Plasmopara halstedii, Puccinia helianthi, or Orobanche cumana have been successfully used in marker-assisted breeding programs for years. However, agronomically important complex quantitative traits like yield, heterosis, drought tolerance, oil content or selection for disease resistance, e.g., against Sclerotinia sclerotiorum have been challenging and will require genome-wide approaches. Plant genetic resources for sunflower are being collected and conserved worldwide that represent valuable resources to study complex traits. Sunflower association panels provide the basis for genome-wide association studies, overcoming disadvantages of biparental populations. Advances in technologies and the availability of the sunflower genome sequence made novel approaches on the whole genome level possible. Genotype-by-sequencing, and whole genome sequencing based on next generation sequencing technologies facilitated the production of large amounts of SNP markers for high density maps as well as SNP arrays and allowed genome-wide association studies and genomic selection in sunflower. Genome wide or candidate gene based association studies have been performed for traits like branching, flowering time, resistance to Sclerotinia head and stalk rot. First steps in genomic selection with regard to hybrid performance and hybrid oil content have shown that genomic selection can successfully address complex quantitative traits in sunflower and will help to speed up sunflower breeding programs in the future. To make sunflower more competitive toward other oil crops higher levels of resistance against pathogens and better yield performance are required. In addition, optimizing plant architecture toward a more complex growth type for higher plant densities has the potential to considerably increase yields per hectare. Integrative approaches combining omic technologies (genomics, transcriptomics, proteomics, metabolomics and phenomics) using bioinformatic tools will facilitate the identification of target genes and markers for complex traits and will give a better insight into the mechanisms behind the traits.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Plant Science
T1  - Sunflower Hybrid Breeding: From Markers to Genomic Selection
VL  - 8
DO  - 10.3389/fpls.2017.02238
ER  - 

@article{
author = "Dimitrijević, Aleksandra and Horn, Renate",
year = "2018",
abstract = "In sunflower, molecular markers for simple traits as, e.g., fertility restoration, high oleic acid content, herbicide tolerance or resistances to Plasmopara halstedii, Puccinia helianthi, or Orobanche cumana have been successfully used in marker-assisted breeding programs for years. However, agronomically important complex quantitative traits like yield, heterosis, drought tolerance, oil content or selection for disease resistance, e.g., against Sclerotinia sclerotiorum have been challenging and will require genome-wide approaches. Plant genetic resources for sunflower are being collected and conserved worldwide that represent valuable resources to study complex traits. Sunflower association panels provide the basis for genome-wide association studies, overcoming disadvantages of biparental populations. Advances in technologies and the availability of the sunflower genome sequence made novel approaches on the whole genome level possible. Genotype-by-sequencing, and whole genome sequencing based on next generation sequencing technologies facilitated the production of large amounts of SNP markers for high density maps as well as SNP arrays and allowed genome-wide association studies and genomic selection in sunflower. Genome wide or candidate gene based association studies have been performed for traits like branching, flowering time, resistance to Sclerotinia head and stalk rot. First steps in genomic selection with regard to hybrid performance and hybrid oil content have shown that genomic selection can successfully address complex quantitative traits in sunflower and will help to speed up sunflower breeding programs in the future. To make sunflower more competitive toward other oil crops higher levels of resistance against pathogens and better yield performance are required. In addition, optimizing plant architecture toward a more complex growth type for higher plant densities has the potential to considerably increase yields per hectare. Integrative approaches combining omic technologies (genomics, transcriptomics, proteomics, metabolomics and phenomics) using bioinformatic tools will facilitate the identification of target genes and markers for complex traits and will give a better insight into the mechanisms behind the traits.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Plant Science",
title = "Sunflower Hybrid Breeding: From Markers to Genomic Selection",
volume = "8",
doi = "10.3389/fpls.2017.02238"
}

Dimitrijević, A.,& Horn, R.. (2018). Sunflower Hybrid Breeding: From Markers to Genomic Selection. in Frontiers in Plant Science
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fpls.2017.02238

Dimitrijević A, Horn R. Sunflower Hybrid Breeding: From Markers to Genomic Selection. in Frontiers in Plant Science. 2018;8.
doi:10.3389/fpls.2017.02238 .

Dimitrijević, Aleksandra, Horn, Renate, "Sunflower Hybrid Breeding: From Markers to Genomic Selection" in Frontiers in Plant Science, 8 (2018),
https://doi.org/10.3389/fpls.2017.02238 . .