KASP markers specific for the fertility restorer locus Rf1 and application for genetic purity testing in sunflowers (Helianthus annuus L.)
Аутори
Radanović, AleksandraSprycha, Yves
Jocković, Milan
Sundt, Monja
Miladinović, Dragana
Jansen, Constantin
Horn, Renate
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Single nucleotide polymorphisms (SNPs) were significantly associated with fertility restoration of cytoplasmic male sterility (CMS) PET1 by the restorer gene Rf1. For these SNPs, four Kompetitive allele-specific PCR (KASP) markers were successfully designed. The KASP markers cover the fertility restorer locus Rf1, spanning about 3 Mb, and clearly differentiate restorer and maintainer lines. For genetic purity testing in sunflower hybrid production, the efficiency for detecting contaminations in samples was simulated using mixtures of hypocotyls or leaves. Contaminations of restorer lines with 1%, 3%, 5%, 10%, and 50% of maintainer lines were screened with all four KASP markers. Contaminations of 10% could be clearly detected in pools of 100 plants. Contaminations below this level require detection on a single plant level. For single plant detections, ethyl methanesulfonate-treated sunflower F1 hybrids, which had been phenotypically evaluated for male sterility (potential mutation in th...e Rf1 gene) were screened. Nine identified either partially male-sterile or male-sterile plants were analyzed with all four KASP markers and only one proved to be a hybrid with a mutation, seven were male-sterile contaminants in the F1 seeds used (1.6%) and one a recombinant plant. The four KASP markers should be valuable tools for marker-assisted selection (MAS) in sunflower breeding regarding the restorer locus Rf1.
Кључне речи:
sunflower / single nucleotide polymorphism / candidate gene / EMS mutagenesis / genetic purity / hybrid breeding / KASP marker / marker-assisted selection / CMS PET1 cytoplasm / restorer gene Rf1Извор:
Genes, 2022, 13, 465-Издавач:
- Basel : MDPI
Финансирање / пројекти:
- project OptiArch, funde by Bundesministerium für Ernährung und Landwirtschaft (BMEL) and Gemeinschaft zur Förderung von Pflanzeninnovation (GFPi) under the guidance of the Fachagentur für Nachwachsende Rohstoffe (Agency for Renewable Resources) (Grant no. 22025215)
- Bilateral project Germany-Serbia (No. 451-03-01732/2017-09/3 and project code 57393592)
- OAP fund of the University of Rostock
DOI: 10.3390/genes13030465
ISSN: 2073-4425
WoS: 000775305700001
Scopus: 2-s2.0-85126438133
Колекције
Институција/група
FiVeRTY - JOUR AU - Radanović, Aleksandra AU - Sprycha, Yves AU - Jocković, Milan AU - Sundt, Monja AU - Miladinović, Dragana AU - Jansen, Constantin AU - Horn, Renate PY - 2022 UR - http://fiver.ifvcns.rs/handle/123456789/2655 AB - Single nucleotide polymorphisms (SNPs) were significantly associated with fertility restoration of cytoplasmic male sterility (CMS) PET1 by the restorer gene Rf1. For these SNPs, four Kompetitive allele-specific PCR (KASP) markers were successfully designed. The KASP markers cover the fertility restorer locus Rf1, spanning about 3 Mb, and clearly differentiate restorer and maintainer lines. For genetic purity testing in sunflower hybrid production, the efficiency for detecting contaminations in samples was simulated using mixtures of hypocotyls or leaves. Contaminations of restorer lines with 1%, 3%, 5%, 10%, and 50% of maintainer lines were screened with all four KASP markers. Contaminations of 10% could be clearly detected in pools of 100 plants. Contaminations below this level require detection on a single plant level. For single plant detections, ethyl methanesulfonate-treated sunflower F1 hybrids, which had been phenotypically evaluated for male sterility (potential mutation in the Rf1 gene) were screened. Nine identified either partially male-sterile or male-sterile plants were analyzed with all four KASP markers and only one proved to be a hybrid with a mutation, seven were male-sterile contaminants in the F1 seeds used (1.6%) and one a recombinant plant. The four KASP markers should be valuable tools for marker-assisted selection (MAS) in sunflower breeding regarding the restorer locus Rf1. PB - Basel : MDPI T2 - Genes T1 - KASP markers specific for the fertility restorer locus Rf1 and application for genetic purity testing in sunflowers (Helianthus annuus L.) SP - 465 VL - 13 DO - 10.3390/genes13030465 ER -
@article{ author = "Radanović, Aleksandra and Sprycha, Yves and Jocković, Milan and Sundt, Monja and Miladinović, Dragana and Jansen, Constantin and Horn, Renate", year = "2022", abstract = "Single nucleotide polymorphisms (SNPs) were significantly associated with fertility restoration of cytoplasmic male sterility (CMS) PET1 by the restorer gene Rf1. For these SNPs, four Kompetitive allele-specific PCR (KASP) markers were successfully designed. The KASP markers cover the fertility restorer locus Rf1, spanning about 3 Mb, and clearly differentiate restorer and maintainer lines. For genetic purity testing in sunflower hybrid production, the efficiency for detecting contaminations in samples was simulated using mixtures of hypocotyls or leaves. Contaminations of restorer lines with 1%, 3%, 5%, 10%, and 50% of maintainer lines were screened with all four KASP markers. Contaminations of 10% could be clearly detected in pools of 100 plants. Contaminations below this level require detection on a single plant level. For single plant detections, ethyl methanesulfonate-treated sunflower F1 hybrids, which had been phenotypically evaluated for male sterility (potential mutation in the Rf1 gene) were screened. Nine identified either partially male-sterile or male-sterile plants were analyzed with all four KASP markers and only one proved to be a hybrid with a mutation, seven were male-sterile contaminants in the F1 seeds used (1.6%) and one a recombinant plant. The four KASP markers should be valuable tools for marker-assisted selection (MAS) in sunflower breeding regarding the restorer locus Rf1.", publisher = "Basel : MDPI", journal = "Genes", title = "KASP markers specific for the fertility restorer locus Rf1 and application for genetic purity testing in sunflowers (Helianthus annuus L.)", pages = "465", volume = "13", doi = "10.3390/genes13030465" }
Radanović, A., Sprycha, Y., Jocković, M., Sundt, M., Miladinović, D., Jansen, C.,& Horn, R.. (2022). KASP markers specific for the fertility restorer locus Rf1 and application for genetic purity testing in sunflowers (Helianthus annuus L.). in Genes Basel : MDPI., 13, 465. https://doi.org/10.3390/genes13030465
Radanović A, Sprycha Y, Jocković M, Sundt M, Miladinović D, Jansen C, Horn R. KASP markers specific for the fertility restorer locus Rf1 and application for genetic purity testing in sunflowers (Helianthus annuus L.). in Genes. 2022;13:465. doi:10.3390/genes13030465 .
Radanović, Aleksandra, Sprycha, Yves, Jocković, Milan, Sundt, Monja, Miladinović, Dragana, Jansen, Constantin, Horn, Renate, "KASP markers specific for the fertility restorer locus Rf1 and application for genetic purity testing in sunflowers (Helianthus annuus L.)" in Genes, 13 (2022):465, https://doi.org/10.3390/genes13030465 . .