TY  - JOUR
AU  - Vlajić, Slobodan
AU  - Iličić, Renata
AU  - Gvozdanović-Varga, Jelica
AU  - Maširević, Stevan
AU  - Kozoderović, Gordana
AU  - Ignjatov, Maja
AU  - Jošić, Dragana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3470
AB  - Xanthomonas campestris pv. campestris (Xcc) is a harmful pathogen of the Brassicaceae agricultural plants in Serbian agro-ecological conditions. The bacterium survives in a soil, plant residues, seeds, wild, and weedy plants. The aim of this study was to characterize Xcc isolates that persist in field soil and seeds where they represent a risk to cause disease of Brassicaceae plants. Collection of soil samples was carried out in the period 2015 - 2017 in four locations regions Vojvodina with cabbage cultivation in monoculture or with crop rotations. The cabbage seed samples were collected in the same period in Futog and Despotovo locations (agricultural farms) and in local farmer markets. All isolations were done on SX and YDC agar plates with the addition of cycloheximide with 250 mg/l. Based on the pathogenicity tests, morphological and biochemical characteristics, 28 identified Xcc isolates were selected, 12 from soil and 16 from seeds. Molecular confirmation of the Xcc isolates was done using multiplex PCR (m-PCR) with Xcc-specific primer sets DLH 120/125 and Zup 2309/2310. In all selected isolates and in the reference Xcc strain NCPPB1144 the expected 370 and 619 bp PCR fragments were amplified. Characterization of isolates by rep-PCR fingerprinting using BOX A1R and (GTG)5 primers resulted in informative patterns. Cluster analysis grouped the strain Xcc NCPPB 1144 together with all Xcc isolates, while other tested Xanthomonads were clustered separately. Based on the similarity level of 90%, we obtained three out of 12 as representative isolates from soil and four out of 16 representative seeds isolates. The characterization of Xcc isolates from soil and seeds will enable their comparison with those pathogenic isolated from leaves of Brassicaceae plants.
PB  - United Arab Emirates University
T2  - Emirates Journal of Food and Agriculture
T1  - Study of Xanthomonas campestris pv. campestris isolates originating from soil and Brassica spp. seeds
EP  - 835
IS  - 10
SP  - 827
VL  - 35
DO  - 10.9755/ejfa.2022.v34.i10.2923
ER  - 

@article{
author = "Vlajić, Slobodan and Iličić, Renata and Gvozdanović-Varga, Jelica and Maširević, Stevan and Kozoderović, Gordana and Ignjatov, Maja and Jošić, Dragana",
year = "2022",
abstract = "Xanthomonas campestris pv. campestris (Xcc) is a harmful pathogen of the Brassicaceae agricultural plants in Serbian agro-ecological conditions. The bacterium survives in a soil, plant residues, seeds, wild, and weedy plants. The aim of this study was to characterize Xcc isolates that persist in field soil and seeds where they represent a risk to cause disease of Brassicaceae plants. Collection of soil samples was carried out in the period 2015 - 2017 in four locations regions Vojvodina with cabbage cultivation in monoculture or with crop rotations. The cabbage seed samples were collected in the same period in Futog and Despotovo locations (agricultural farms) and in local farmer markets. All isolations were done on SX and YDC agar plates with the addition of cycloheximide with 250 mg/l. Based on the pathogenicity tests, morphological and biochemical characteristics, 28 identified Xcc isolates were selected, 12 from soil and 16 from seeds. Molecular confirmation of the Xcc isolates was done using multiplex PCR (m-PCR) with Xcc-specific primer sets DLH 120/125 and Zup 2309/2310. In all selected isolates and in the reference Xcc strain NCPPB1144 the expected 370 and 619 bp PCR fragments were amplified. Characterization of isolates by rep-PCR fingerprinting using BOX A1R and (GTG)5 primers resulted in informative patterns. Cluster analysis grouped the strain Xcc NCPPB 1144 together with all Xcc isolates, while other tested Xanthomonads were clustered separately. Based on the similarity level of 90%, we obtained three out of 12 as representative isolates from soil and four out of 16 representative seeds isolates. The characterization of Xcc isolates from soil and seeds will enable their comparison with those pathogenic isolated from leaves of Brassicaceae plants.",
publisher = "United Arab Emirates University",
journal = "Emirates Journal of Food and Agriculture",
title = "Study of Xanthomonas campestris pv. campestris isolates originating from soil and Brassica spp. seeds",
pages = "835-827",
number = "10",
volume = "35",
doi = "10.9755/ejfa.2022.v34.i10.2923"
}

Vlajić, S., Iličić, R., Gvozdanović-Varga, J., Maširević, S., Kozoderović, G., Ignjatov, M.,& Jošić, D.. (2022). Study of Xanthomonas campestris pv. campestris isolates originating from soil and Brassica spp. seeds. in Emirates Journal of Food and Agriculture
United Arab Emirates University., 35(10), 827-835.
https://doi.org/10.9755/ejfa.2022.v34.i10.2923

Vlajić S, Iličić R, Gvozdanović-Varga J, Maširević S, Kozoderović G, Ignjatov M, Jošić D. Study of Xanthomonas campestris pv. campestris isolates originating from soil and Brassica spp. seeds. in Emirates Journal of Food and Agriculture. 2022;35(10):827-835.
doi:10.9755/ejfa.2022.v34.i10.2923 .

Vlajić, Slobodan, Iličić, Renata, Gvozdanović-Varga, Jelica, Maširević, Stevan, Kozoderović, Gordana, Ignjatov, Maja, Jošić, Dragana, "Study of Xanthomonas campestris pv. campestris isolates originating from soil and Brassica spp. seeds" in Emirates Journal of Food and Agriculture, 35, no. 10 (2022):827-835,
https://doi.org/10.9755/ejfa.2022.v34.i10.2923 . .

TY  - CONF
AU  - Vlajić, Slobodan
AU  - Iličić, Renata
AU  - Maširević, Stevan
AU  - Gvozdanović-Varga, Jelica
AU  - Kozoderović, Gordana
AU  - Jošić, Dragana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/3075
AB  - Brassicas are a significant source of edible vegetables and is the third most important source of oil on the world level. Black rot, caused by the bacterium Xanthomonas campestris pv. campestris (XCC) represents the most destructive Brassicas diseases, generally. The bacteria is widespread on all continents, and is present in our country, especially at the places where Brassicas are grown in monoculture. Particularly susceptible are cabbage local genotypes, which are highly appreciated by consumers. Given the importance, frequency and distribution of XCC, there was a need to study the diversity of this pathogen on cabbage, cauliflower, kale, broccoli and oil seed rape. Monitoring was conducted from 2014 to 2017 on the territory of the Republic of Serbia. From different Brassicas 82 bacterial isolates from leaves were collected. All isolates form yellow colonies on the YDC medium and based on biochemical-physiological characteristics were identified preliminarily as XCC.
PB  - Belgrade : Serbian Genetic Society
C3  - Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja
T1  - Genodiversity of Xanthomonas campestris pv. campestris isolated from leaves of Brassicaceae plants
EP  - 252
SP  - 252
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3075
ER  - 

@conference{
author = "Vlajić, Slobodan and Iličić, Renata and Maširević, Stevan and Gvozdanović-Varga, Jelica and Kozoderović, Gordana and Jošić, Dragana",
year = "2019",
abstract = "Brassicas are a significant source of edible vegetables and is the third most important source of oil on the world level. Black rot, caused by the bacterium Xanthomonas campestris pv. campestris (XCC) represents the most destructive Brassicas diseases, generally. The bacteria is widespread on all continents, and is present in our country, especially at the places where Brassicas are grown in monoculture. Particularly susceptible are cabbage local genotypes, which are highly appreciated by consumers. Given the importance, frequency and distribution of XCC, there was a need to study the diversity of this pathogen on cabbage, cauliflower, kale, broccoli and oil seed rape. Monitoring was conducted from 2014 to 2017 on the territory of the Republic of Serbia. From different Brassicas 82 bacterial isolates from leaves were collected. All isolates form yellow colonies on the YDC medium and based on biochemical-physiological characteristics were identified preliminarily as XCC.",
publisher = "Belgrade : Serbian Genetic Society",
journal = "Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja",
title = "Genodiversity of Xanthomonas campestris pv. campestris isolated from leaves of Brassicaceae plants",
pages = "252-252",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3075"
}

Vlajić, S., Iličić, R., Maširević, S., Gvozdanović-Varga, J., Kozoderović, G.,& Jošić, D.. (2019). Genodiversity of Xanthomonas campestris pv. campestris isolated from leaves of Brassicaceae plants. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja
Belgrade : Serbian Genetic Society., 252-252.
https://hdl.handle.net/21.15107/rcub_fiver_3075

Vlajić S, Iličić R, Maširević S, Gvozdanović-Varga J, Kozoderović G, Jošić D. Genodiversity of Xanthomonas campestris pv. campestris isolated from leaves of Brassicaceae plants. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja. 2019;:252-252.
https://hdl.handle.net/21.15107/rcub_fiver_3075 .

Vlajić, Slobodan, Iličić, Renata, Maširević, Stevan, Gvozdanović-Varga, Jelica, Kozoderović, Gordana, Jošić, Dragana, "Genodiversity of Xanthomonas campestris pv. campestris isolated from leaves of Brassicaceae plants" in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja (2019):252-252,
https://hdl.handle.net/21.15107/rcub_fiver_3075 .