TY  - JOUR
AU  - Ilić, Marko
AU  - Pastor, Kristian
AU  - Ilić, Aleksandra
AU  - Vasić, Mirjana
AU  - Nastić, Nataša
AU  - Vujić, Đura
AU  - Ačanski, Marijana
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/4219
AB  - This study presents a tentative analysis of the lipid composition of 47 legume samples, encompassing species such as Phaseolus spp., Vicia spp., Pisum spp., and Lathyrus spp. Lipid extraction and GC/MS (gas chromatography with mass spectrometric detection) analysis were conducted, followed by multivariate statistical methods for data interpretation. Hierarchical Cluster Analysis (HCA) revealed two major clusters, distinguishing beans and snap beans (Phaseolus spp.) from faba beans (Vicia faba), peas (Pisum sativum), and grass peas (Lathyrus sativus). Principal Component Analysis (PCA) yielded 2D and 3D score plots, effectively discriminating legume species. Linear Discriminant Analysis (LDA) achieved a 100% accurate classification of the training set and a 90% accuracy of the test set. The lipid-based fingerprinting elucidated compounds crucial for discrimination. Both PCA and LDA biplots highlighted squalene and fatty acid methyl esters (FAMEs) of 9,12,15-octadecatrienoic acid (C18:3) and 5,11,14,17-eicosatetraenoic acid (C20:4) as influential in the clustering of beans and snap beans. Unique compounds, including 13-docosenoic acid (C22:1) and γ-tocopherol, O-methyl-, characterized grass pea samples. Faba bean samples were discriminated by FAMEs of heneicosanoic acid (C21:0) and oxiraneoctanoic acid, 3-octyl- (C18-ox). However, C18-ox was also found in pea samples, but in significantly lower amounts. This research demonstrates the efficacy of lipid analysis coupled with multivariate statistics for accurate differentiation and classification of legumes, according to their botanical origins.
PB  - Basel : MDPI
T2  - Foods - Basel
T1  - Legume Fingerprinting through Lipid Composition: Utilizing GC/MS with Multivariate Statistics
IS  - 24
SP  - 4420
VL  - 12
DO  - 10.3390/foods12244420
ER  - 

@article{
author = "Ilić, Marko and Pastor, Kristian and Ilić, Aleksandra and Vasić, Mirjana and Nastić, Nataša and Vujić, Đura and Ačanski, Marijana",
year = "2023",
abstract = "This study presents a tentative analysis of the lipid composition of 47 legume samples, encompassing species such as Phaseolus spp., Vicia spp., Pisum spp., and Lathyrus spp. Lipid extraction and GC/MS (gas chromatography with mass spectrometric detection) analysis were conducted, followed by multivariate statistical methods for data interpretation. Hierarchical Cluster Analysis (HCA) revealed two major clusters, distinguishing beans and snap beans (Phaseolus spp.) from faba beans (Vicia faba), peas (Pisum sativum), and grass peas (Lathyrus sativus). Principal Component Analysis (PCA) yielded 2D and 3D score plots, effectively discriminating legume species. Linear Discriminant Analysis (LDA) achieved a 100% accurate classification of the training set and a 90% accuracy of the test set. The lipid-based fingerprinting elucidated compounds crucial for discrimination. Both PCA and LDA biplots highlighted squalene and fatty acid methyl esters (FAMEs) of 9,12,15-octadecatrienoic acid (C18:3) and 5,11,14,17-eicosatetraenoic acid (C20:4) as influential in the clustering of beans and snap beans. Unique compounds, including 13-docosenoic acid (C22:1) and γ-tocopherol, O-methyl-, characterized grass pea samples. Faba bean samples were discriminated by FAMEs of heneicosanoic acid (C21:0) and oxiraneoctanoic acid, 3-octyl- (C18-ox). However, C18-ox was also found in pea samples, but in significantly lower amounts. This research demonstrates the efficacy of lipid analysis coupled with multivariate statistics for accurate differentiation and classification of legumes, according to their botanical origins.",
publisher = "Basel : MDPI",
journal = "Foods - Basel",
title = "Legume Fingerprinting through Lipid Composition: Utilizing GC/MS with Multivariate Statistics",
number = "24",
pages = "4420",
volume = "12",
doi = "10.3390/foods12244420"
}

Ilić, M., Pastor, K., Ilić, A., Vasić, M., Nastić, N., Vujić, Đ.,& Ačanski, M.. (2023). Legume Fingerprinting through Lipid Composition: Utilizing GC/MS with Multivariate Statistics. in Foods - Basel
Basel : MDPI., 12(24), 4420.
https://doi.org/10.3390/foods12244420

Ilić M, Pastor K, Ilić A, Vasić M, Nastić N, Vujić Đ, Ačanski M. Legume Fingerprinting through Lipid Composition: Utilizing GC/MS with Multivariate Statistics. in Foods - Basel. 2023;12(24):4420.
doi:10.3390/foods12244420 .

Ilić, Marko, Pastor, Kristian, Ilić, Aleksandra, Vasić, Mirjana, Nastić, Nataša, Vujić, Đura, Ačanski, Marijana, "Legume Fingerprinting through Lipid Composition: Utilizing GC/MS with Multivariate Statistics" in Foods - Basel, 12, no. 24 (2023):4420,
https://doi.org/10.3390/foods12244420 . .

TY  - CONF
AU  - Ilić, Marko
AU  - Pastor, Kristian
AU  - Marjanović-Jeromela, Ana
AU  - Romanić, Ranko
AU  - Miklič, Vladimir
AU  - Vujić, Đura
AU  - Ačanski, Marijana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3267
AB  - Vegetable oils have different sensory, physico-chemical and nutritive characteristics and shelf life, which is why the use of only one vegetable oil in diet is often not sufficient. Oil blending represents a very simple way to employ various characteristic properties of a large number of vegetable oils and is also an economical way to change sensory, physicochemical and nutritive characteristics and improve oxidative stability. Blending of various vegetable oils can change the fatty acid compositions and provide a higher content and different composition of natural antioxidants and bioactive lipids, thereby improving the nutritive value and stability, i.e. shelf life of the obtained oil. Sunflower oil is one of the top five edible oils in the world, having a full priority in Serbia, due to the high availability of raw materials over others. Due to the presence of essential linoleic (omega 6) fatty acid and alpha-tocopherol (vitamin E), it has a high nutritive value, while due to its extremely good nutritive characteristics and the presence of bioactive components, such as essential alpha-linolenic (omega 3) fatty acid, flaxseed oil has been increasingly used in the diet in recent years. The aim of this paper is to determine the possibility of applying a gas chromatography device with a mass spectrometric detector (GC-MS) coupled to a newly developed mathematical model to determine the proportion of individual oils in blended oils. Nine blended oils with different proportions (0-100% w/w) of refined sunflower oil and cold pressed flaxseed oil, as well as pure refined sunflower oil and cold pressed flaxseed oil, were prepared and analyzed by GC-MS._The peaks of the molecular ions of specific fatty acids were integrated. Based on the surface areas of these peaks, a novel mathematical model was developed to determine the proportion of individual oils in the blends. All obtained experimental values are consistent with the actual mass fractions of individual oils in the oil blends. Very high correlation coefficient of 0.9964 between experimentally determined and actual proportions of individual oils in blends is an indicator of the applicability of the developed method presented in this work.
PB  - Paris : International Sunflower Association
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia
T1  - A Novel Method of Determination of Individual Oil Content in Sunflower and Flaxseed Oil Blends
EP  - 188
SP  - 188
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3267
ER  - 

@conference{
author = "Ilić, Marko and Pastor, Kristian and Marjanović-Jeromela, Ana and Romanić, Ranko and Miklič, Vladimir and Vujić, Đura and Ačanski, Marijana",
year = "2022",
abstract = "Vegetable oils have different sensory, physico-chemical and nutritive characteristics and shelf life, which is why the use of only one vegetable oil in diet is often not sufficient. Oil blending represents a very simple way to employ various characteristic properties of a large number of vegetable oils and is also an economical way to change sensory, physicochemical and nutritive characteristics and improve oxidative stability. Blending of various vegetable oils can change the fatty acid compositions and provide a higher content and different composition of natural antioxidants and bioactive lipids, thereby improving the nutritive value and stability, i.e. shelf life of the obtained oil. Sunflower oil is one of the top five edible oils in the world, having a full priority in Serbia, due to the high availability of raw materials over others. Due to the presence of essential linoleic (omega 6) fatty acid and alpha-tocopherol (vitamin E), it has a high nutritive value, while due to its extremely good nutritive characteristics and the presence of bioactive components, such as essential alpha-linolenic (omega 3) fatty acid, flaxseed oil has been increasingly used in the diet in recent years. The aim of this paper is to determine the possibility of applying a gas chromatography device with a mass spectrometric detector (GC-MS) coupled to a newly developed mathematical model to determine the proportion of individual oils in blended oils. Nine blended oils with different proportions (0-100% w/w) of refined sunflower oil and cold pressed flaxseed oil, as well as pure refined sunflower oil and cold pressed flaxseed oil, were prepared and analyzed by GC-MS._The peaks of the molecular ions of specific fatty acids were integrated. Based on the surface areas of these peaks, a novel mathematical model was developed to determine the proportion of individual oils in the blends. All obtained experimental values are consistent with the actual mass fractions of individual oils in the oil blends. Very high correlation coefficient of 0.9964 between experimentally determined and actual proportions of individual oils in blends is an indicator of the applicability of the developed method presented in this work.",
publisher = "Paris : International Sunflower Association, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia",
title = "A Novel Method of Determination of Individual Oil Content in Sunflower and Flaxseed Oil Blends",
pages = "188-188",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3267"
}

Ilić, M., Pastor, K., Marjanović-Jeromela, A., Romanić, R., Miklič, V., Vujić, Đ.,& Ačanski, M.. (2022). A Novel Method of Determination of Individual Oil Content in Sunflower and Flaxseed Oil Blends. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia
Paris : International Sunflower Association., 188-188.
https://hdl.handle.net/21.15107/rcub_fiver_3267

Ilić M, Pastor K, Marjanović-Jeromela A, Romanić R, Miklič V, Vujić Đ, Ačanski M. A Novel Method of Determination of Individual Oil Content in Sunflower and Flaxseed Oil Blends. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia. 2022;:188-188.
https://hdl.handle.net/21.15107/rcub_fiver_3267 .

Ilić, Marko, Pastor, Kristian, Marjanović-Jeromela, Ana, Romanić, Ranko, Miklič, Vladimir, Vujić, Đura, Ačanski, Marijana, "A Novel Method of Determination of Individual Oil Content in Sunflower and Flaxseed Oil Blends" in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia (2022):188-188,
https://hdl.handle.net/21.15107/rcub_fiver_3267 .

TY  - CONF
AU  - Ilić, Marko
AU  - Pastor, Kristian
AU  - Marjanović-Jeromela, Ana
AU  - Romanić, Ranko
AU  - Vujić, Đura
AU  - Ačanski, Marijana
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2160
AB  - Food authentication is the process by which a food product is verified as complying with its label description. The characterization and authentication of oils and fats is a subject of great importance for market and health aspects. The aim of this paper was to determine the possibility of applying a gas chromatography device with a mass spectrometric detector (GC-MS) coupled to a newly developed mathematical model to determine the proportion of individual oils in simulated blended oil samples.
AB  - Autentifikacija hrane je postupak kojim se potvrđuje usaglašenost prehrambenog proizvoda sa 
njegovim opisom na etiketi. Karakterizacija i autentifikacija ulja i masti je tema od velike važnosti 
sa tržišnog i zdravstvenog aspekta. Cilj ovog rada bio je da se utvrdi mogućnost primene uređaja za gasnu hromatografiju sa masenim spektrometrom kao detektorom (GC-MS) i novorazvijenog matematičkog modela za određivanje udela pojedinačnih ulja u simuliranim uzorcima smeša ulja.
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi
C3  - Book of abstracts, 7th International conference sustainable postharvest and food technologies - INOPTEP 2021, 18-23. april 2021, Vršac, Serbia
T1  - A new method for the proportion estimation in edible oil blends: sunflower and rapeseed oil
T1  - Novi metod procene sastava smeša jestivih ulja: ulje suncokreta i uljane repice
EP  - 41
SP  - 40
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2160
ER  - 

@conference{
author = "Ilić, Marko and Pastor, Kristian and Marjanović-Jeromela, Ana and Romanić, Ranko and Vujić, Đura and Ačanski, Marijana",
year = "2021",
abstract = "Food authentication is the process by which a food product is verified as complying with its label description. The characterization and authentication of oils and fats is a subject of great importance for market and health aspects. The aim of this paper was to determine the possibility of applying a gas chromatography device with a mass spectrometric detector (GC-MS) coupled to a newly developed mathematical model to determine the proportion of individual oils in simulated blended oil samples., Autentifikacija hrane je postupak kojim se potvrđuje usaglašenost prehrambenog proizvoda sa 
njegovim opisom na etiketi. Karakterizacija i autentifikacija ulja i masti je tema od velike važnosti 
sa tržišnog i zdravstvenog aspekta. Cilj ovog rada bio je da se utvrdi mogućnost primene uređaja za gasnu hromatografiju sa masenim spektrometrom kao detektorom (GC-MS) i novorazvijenog matematičkog modela za određivanje udela pojedinačnih ulja u simuliranim uzorcima smeša ulja.",
publisher = "Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi",
journal = "Book of abstracts, 7th International conference sustainable postharvest and food technologies - INOPTEP 2021, 18-23. april 2021, Vršac, Serbia",
title = "A new method for the proportion estimation in edible oil blends: sunflower and rapeseed oil, Novi metod procene sastava smeša jestivih ulja: ulje suncokreta i uljane repice",
pages = "41-40",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2160"
}

Ilić, M., Pastor, K., Marjanović-Jeromela, A., Romanić, R., Vujić, Đ.,& Ačanski, M.. (2021). A new method for the proportion estimation in edible oil blends: sunflower and rapeseed oil. in Book of abstracts, 7th International conference sustainable postharvest and food technologies - INOPTEP 2021, 18-23. april 2021, Vršac, Serbia
Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi., 40-41.
https://hdl.handle.net/21.15107/rcub_fiver_2160

Ilić M, Pastor K, Marjanović-Jeromela A, Romanić R, Vujić Đ, Ačanski M. A new method for the proportion estimation in edible oil blends: sunflower and rapeseed oil. in Book of abstracts, 7th International conference sustainable postharvest and food technologies - INOPTEP 2021, 18-23. april 2021, Vršac, Serbia. 2021;:40-41.
https://hdl.handle.net/21.15107/rcub_fiver_2160 .

Ilić, Marko, Pastor, Kristian, Marjanović-Jeromela, Ana, Romanić, Ranko, Vujić, Đura, Ačanski, Marijana, "A new method for the proportion estimation in edible oil blends: sunflower and rapeseed oil" in Book of abstracts, 7th International conference sustainable postharvest and food technologies - INOPTEP 2021, 18-23. april 2021, Vršac, Serbia (2021):40-41,
https://hdl.handle.net/21.15107/rcub_fiver_2160 .

TY  - CONF
AU  - Bursić, Vojislava
AU  - Vuković, Gorica
AU  - Čabilovski, Ranko
AU  - Zeremski, Tijana
AU  - Ilić, Marko
AU  - Baličević, Renata
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/4544
AB  - The LC–MS/MS was applied for the detection of pesticide residues in watermelons. Since watermelons are predominantly used as fresh food and to a lesser extent in food processing there is a justified concern that, due to treatments, they can contain pesticide residues above the maximum residue levels – MRLs. The pesticide extraction was carried out by QuEChERS method. The samples were tested regarding the content of 55 pesticides with the carbofuran-D3 as internal standard. The linearity was studied in the range of 0.01–0.50 mg/kg and the determination coefficients (R2) were higher than 0.99 for all the investigated pesticides. The calibration was performed as matrix calibration, by means of spiking the calibration samples before the extraction and preparing them in the same way as the test samples. The recovery data were obtained by spiking blank samples at three concentration levels (0.01, 0.05 and 0.1 mg/kg) yielding recoveries in the range of 61.0–114.2% with the relative standard deviation (RSD) less than 13%. The limits of quantification (LOQs) were established as 0.01 mg/kg. The multiple detections were confirmed in the analysed samples. The most frequently detected pesticides were carbendazim, acetamiprid, tefluthrin and fenpropimorph. In only one sample the concentrations of carbendazim and tefluthrin were above the MRLs.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor
T1  - Determination of pesticide residues in watermelons by LC-MS/MS
EP  - 357
SP  - 353
UR  - https://hdl.handle.net/21.15107/rcub_fiver_4544
ER  - 

@conference{
author = "Bursić, Vojislava and Vuković, Gorica and Čabilovski, Ranko and Zeremski, Tijana and Ilić, Marko and Baličević, Renata",
year = "2015",
abstract = "The LC–MS/MS was applied for the detection of pesticide residues in watermelons. Since watermelons are predominantly used as fresh food and to a lesser extent in food processing there is a justified concern that, due to treatments, they can contain pesticide residues above the maximum residue levels – MRLs. The pesticide extraction was carried out by QuEChERS method. The samples were tested regarding the content of 55 pesticides with the carbofuran-D3 as internal standard. The linearity was studied in the range of 0.01–0.50 mg/kg and the determination coefficients (R2) were higher than 0.99 for all the investigated pesticides. The calibration was performed as matrix calibration, by means of spiking the calibration samples before the extraction and preparing them in the same way as the test samples. The recovery data were obtained by spiking blank samples at three concentration levels (0.01, 0.05 and 0.1 mg/kg) yielding recoveries in the range of 61.0–114.2% with the relative standard deviation (RSD) less than 13%. The limits of quantification (LOQs) were established as 0.01 mg/kg. The multiple detections were confirmed in the analysed samples. The most frequently detected pesticides were carbendazim, acetamiprid, tefluthrin and fenpropimorph. In only one sample the concentrations of carbendazim and tefluthrin were above the MRLs.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor",
title = "Determination of pesticide residues in watermelons by LC-MS/MS",
pages = "357-353",
url = "https://hdl.handle.net/21.15107/rcub_fiver_4544"
}

Bursić, V., Vuković, G., Čabilovski, R., Zeremski, T., Ilić, M.,& Baličević, R.. (2015). Determination of pesticide residues in watermelons by LC-MS/MS. in Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor
Beograd : Društvo za zaštitu bilja Srbije., 353-357.
https://hdl.handle.net/21.15107/rcub_fiver_4544

Bursić V, Vuković G, Čabilovski R, Zeremski T, Ilić M, Baličević R. Determination of pesticide residues in watermelons by LC-MS/MS. in Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor. 2015;:353-357.
https://hdl.handle.net/21.15107/rcub_fiver_4544 .

Bursić, Vojislava, Vuković, Gorica, Čabilovski, Ranko, Zeremski, Tijana, Ilić, Marko, Baličević, Renata, "Determination of pesticide residues in watermelons by LC-MS/MS" in Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor (2015):353-357,
https://hdl.handle.net/21.15107/rcub_fiver_4544 .