Identification of biomarkers in hydrosoluble extracts from spelt and wheat cultivated in different production systems
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Bodroža-Solarov, MarijaGrobelnik-Mlakar, Silva
Pezo, Lato
Keleman, Svetlana
Ilin, Sonja
Marić, Boško
Filipčev, Bojana
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BACKGROUND In the present paper, a method for differentiation between common and spelt wheat grown in different farming systems (biodynamic, ecological, integrated, conventional), based on biomarkers identified from aqueous flour extracts (nitrogen and 14 soluble carbohydrates) was employed. RESULTS The identification and determination of soluble carbohydrate content were carried out using gas chromatography-mass spectrometry, with the UV spectrum generated by mass spectrometry for comparison with the WILEY database. Soluble carbohydrates were determined in the peak area between 21.92 and 43.63 min(-1) retention time. The obtained data set was analyzed by multivariate statistical techniques. It was revealed that common wheat exerted a much more pronounced tendency than spelt wheat to be influenced by the farming system. CONCLUSION This differentiation was particularly well visualized after subjecting the data set to principal component analysis (PCA) and cluster analysis (CA). In the P...CA graph, all spelt samples were positioned closer to the corresponding control sample, in contrast to the wheat samples, which were distributed over a huge area in the factor space. CA showed that the spelt samples grown under different farming systems were highly similar and grouped into one cluster. Common wheat samples cultivated under organic, biodynamic and integrated system were similar and represented the second cluster, whereas that cultivated under the conventional system was clearly separated from other samples.
Keywords:
organic / wheat / spelt / hydrosoluble extract / biomarkersSource:
Journal of The Science of Food & Agriculture, 2021Publisher:
- Wiley, Hoboken
Funding / projects:
DOI: 10.1002/jsfa.10971
ISSN: 0022-5142
PubMed: 33274457
WoS: 000599728800001
Scopus: 2-s2.0-85097662246
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FiVeRTY - JOUR AU - Bodroža-Solarov, Marija AU - Grobelnik-Mlakar, Silva AU - Pezo, Lato AU - Keleman, Svetlana AU - Ilin, Sonja AU - Marić, Boško AU - Filipčev, Bojana PY - 2021 UR - http://fiver.ifvcns.rs/handle/123456789/9 AB - BACKGROUND In the present paper, a method for differentiation between common and spelt wheat grown in different farming systems (biodynamic, ecological, integrated, conventional), based on biomarkers identified from aqueous flour extracts (nitrogen and 14 soluble carbohydrates) was employed. RESULTS The identification and determination of soluble carbohydrate content were carried out using gas chromatography-mass spectrometry, with the UV spectrum generated by mass spectrometry for comparison with the WILEY database. Soluble carbohydrates were determined in the peak area between 21.92 and 43.63 min(-1) retention time. The obtained data set was analyzed by multivariate statistical techniques. It was revealed that common wheat exerted a much more pronounced tendency than spelt wheat to be influenced by the farming system. CONCLUSION This differentiation was particularly well visualized after subjecting the data set to principal component analysis (PCA) and cluster analysis (CA). In the PCA graph, all spelt samples were positioned closer to the corresponding control sample, in contrast to the wheat samples, which were distributed over a huge area in the factor space. CA showed that the spelt samples grown under different farming systems were highly similar and grouped into one cluster. Common wheat samples cultivated under organic, biodynamic and integrated system were similar and represented the second cluster, whereas that cultivated under the conventional system was clearly separated from other samples. PB - Wiley, Hoboken T2 - Journal of The Science of Food & Agriculture T1 - Identification of biomarkers in hydrosoluble extracts from spelt and wheat cultivated in different production systems DO - 10.1002/jsfa.10971 ER -
@article{ author = "Bodroža-Solarov, Marija and Grobelnik-Mlakar, Silva and Pezo, Lato and Keleman, Svetlana and Ilin, Sonja and Marić, Boško and Filipčev, Bojana", year = "2021", abstract = "BACKGROUND In the present paper, a method for differentiation between common and spelt wheat grown in different farming systems (biodynamic, ecological, integrated, conventional), based on biomarkers identified from aqueous flour extracts (nitrogen and 14 soluble carbohydrates) was employed. RESULTS The identification and determination of soluble carbohydrate content were carried out using gas chromatography-mass spectrometry, with the UV spectrum generated by mass spectrometry for comparison with the WILEY database. Soluble carbohydrates were determined in the peak area between 21.92 and 43.63 min(-1) retention time. The obtained data set was analyzed by multivariate statistical techniques. It was revealed that common wheat exerted a much more pronounced tendency than spelt wheat to be influenced by the farming system. CONCLUSION This differentiation was particularly well visualized after subjecting the data set to principal component analysis (PCA) and cluster analysis (CA). In the PCA graph, all spelt samples were positioned closer to the corresponding control sample, in contrast to the wheat samples, which were distributed over a huge area in the factor space. CA showed that the spelt samples grown under different farming systems were highly similar and grouped into one cluster. Common wheat samples cultivated under organic, biodynamic and integrated system were similar and represented the second cluster, whereas that cultivated under the conventional system was clearly separated from other samples.", publisher = "Wiley, Hoboken", journal = "Journal of The Science of Food & Agriculture", title = "Identification of biomarkers in hydrosoluble extracts from spelt and wheat cultivated in different production systems", doi = "10.1002/jsfa.10971" }
Bodroža-Solarov, M., Grobelnik-Mlakar, S., Pezo, L., Keleman, S., Ilin, S., Marić, B.,& Filipčev, B.. (2021). Identification of biomarkers in hydrosoluble extracts from spelt and wheat cultivated in different production systems. in Journal of The Science of Food & Agriculture Wiley, Hoboken.. https://doi.org/10.1002/jsfa.10971
Bodroža-Solarov M, Grobelnik-Mlakar S, Pezo L, Keleman S, Ilin S, Marić B, Filipčev B. Identification of biomarkers in hydrosoluble extracts from spelt and wheat cultivated in different production systems. in Journal of The Science of Food & Agriculture. 2021;. doi:10.1002/jsfa.10971 .
Bodroža-Solarov, Marija, Grobelnik-Mlakar, Silva, Pezo, Lato, Keleman, Svetlana, Ilin, Sonja, Marić, Boško, Filipčev, Bojana, "Identification of biomarkers in hydrosoluble extracts from spelt and wheat cultivated in different production systems" in Journal of The Science of Food & Agriculture (2021), https://doi.org/10.1002/jsfa.10971 . .