Use of SSR markers in identification of sunflower isogenic lines in late generations of backcrossing
2010
Аутори
Dimitrijević, AleksandraImerovski, Ivana
Miladinović, Dragana
Tančić, Sonja
Dušanić, Nenad
Jocić, Siniša
Miklič, Vladimir
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In the present study, thirteen SSRs (Simple Sequence Repeats) were employed for screening the level of similarity between sunflower parental lines and progeny obtained in final stages of selection. Another aim of the study was to compare the success of making isogenic lines through selection. Line P (donor line) was used as a donor of downy mildew gene Pl6 and backcrossed with four commercial inbred lines (A, B, C and D) susceptible to downy mildew. HAP3, a DNA-specific primer, was used to test the success of transfer of Pl6 gene from the donor line to the progeny lines in the F6 and F7 generations. It was found that all progeny lines were resistant to downy mildew. Results obtained by using thirteen primers showed that progeny lines were similar to their respective recipient parent, but further studies have to be conducted using a larger number of primers and a larger number of genotypes.
Кључне речи:
sunflower / Helianthus annuus L. / isogenic line / backcross / SSR / downy mildewИзвор:
Helia, 2010, 33, 53, 191-198Издавач:
- Institut za ratarstvo i povrtarstvo, Novi Sad
Финансирање / пројекти:
- Стварање високо продуктивних генотипова сунцокрета (Helianthus annuus L.) (RS-MESTD-MPN2006-2010-20080)
Колекције
Институција/група
FiVeRTY - JOUR AU - Dimitrijević, Aleksandra AU - Imerovski, Ivana AU - Miladinović, Dragana AU - Tančić, Sonja AU - Dušanić, Nenad AU - Jocić, Siniša AU - Miklič, Vladimir PY - 2010 UR - http://fiver.ifvcns.rs/handle/123456789/911 AB - In the present study, thirteen SSRs (Simple Sequence Repeats) were employed for screening the level of similarity between sunflower parental lines and progeny obtained in final stages of selection. Another aim of the study was to compare the success of making isogenic lines through selection. Line P (donor line) was used as a donor of downy mildew gene Pl6 and backcrossed with four commercial inbred lines (A, B, C and D) susceptible to downy mildew. HAP3, a DNA-specific primer, was used to test the success of transfer of Pl6 gene from the donor line to the progeny lines in the F6 and F7 generations. It was found that all progeny lines were resistant to downy mildew. Results obtained by using thirteen primers showed that progeny lines were similar to their respective recipient parent, but further studies have to be conducted using a larger number of primers and a larger number of genotypes. PB - Institut za ratarstvo i povrtarstvo, Novi Sad T2 - Helia T1 - Use of SSR markers in identification of sunflower isogenic lines in late generations of backcrossing EP - 198 IS - 53 SP - 191 VL - 33 DO - 10.2298/HEL1053191D ER -
@article{ author = "Dimitrijević, Aleksandra and Imerovski, Ivana and Miladinović, Dragana and Tančić, Sonja and Dušanić, Nenad and Jocić, Siniša and Miklič, Vladimir", year = "2010", abstract = "In the present study, thirteen SSRs (Simple Sequence Repeats) were employed for screening the level of similarity between sunflower parental lines and progeny obtained in final stages of selection. Another aim of the study was to compare the success of making isogenic lines through selection. Line P (donor line) was used as a donor of downy mildew gene Pl6 and backcrossed with four commercial inbred lines (A, B, C and D) susceptible to downy mildew. HAP3, a DNA-specific primer, was used to test the success of transfer of Pl6 gene from the donor line to the progeny lines in the F6 and F7 generations. It was found that all progeny lines were resistant to downy mildew. Results obtained by using thirteen primers showed that progeny lines were similar to their respective recipient parent, but further studies have to be conducted using a larger number of primers and a larger number of genotypes.", publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad", journal = "Helia", title = "Use of SSR markers in identification of sunflower isogenic lines in late generations of backcrossing", pages = "198-191", number = "53", volume = "33", doi = "10.2298/HEL1053191D" }
Dimitrijević, A., Imerovski, I., Miladinović, D., Tančić, S., Dušanić, N., Jocić, S.,& Miklič, V.. (2010). Use of SSR markers in identification of sunflower isogenic lines in late generations of backcrossing. in Helia Institut za ratarstvo i povrtarstvo, Novi Sad., 33(53), 191-198. https://doi.org/10.2298/HEL1053191D
Dimitrijević A, Imerovski I, Miladinović D, Tančić S, Dušanić N, Jocić S, Miklič V. Use of SSR markers in identification of sunflower isogenic lines in late generations of backcrossing. in Helia. 2010;33(53):191-198. doi:10.2298/HEL1053191D .
Dimitrijević, Aleksandra, Imerovski, Ivana, Miladinović, Dragana, Tančić, Sonja, Dušanić, Nenad, Jocić, Siniša, Miklič, Vladimir, "Use of SSR markers in identification of sunflower isogenic lines in late generations of backcrossing" in Helia, 33, no. 53 (2010):191-198, https://doi.org/10.2298/HEL1053191D . .