Qualitative and quantitative determination of the Kunitz trypsin inhibitor (KTI) in soybean experimental lines is very important in processes of selecting and breeding of new varieties. The total enzyme activity assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and Lab-on-a-Chip (LoaC) method were used for the determination of the presence and quantity of KTI in 15 soybean experimental lines and varieties. From the total trypsin inhibitor enzyme assay, inhibitor activities were registered in all samples, even in a Kunitz variety that was a negative control. The SDS-PAGE method did not detect the presence of the KTI protein band in seven soybean experimental lines and Kunitz variety, while the LoaC method showed the absence of KTI only in the Kunitz variety sample. Results confirmed the superiority of the LoaC method over other two methods in selectivity and sensitivity when KTI determination is concerned. Relationships between the KTI content obtained by the ...LoaC method and total trypsin inhibitor enzyme activity were established and statistically confirmed.
TY - JOUR
AU - Torbica, Aleksandra
AU - Živančev, Dragan
AU - Nikolić, Zorica
AU - Đorđević, Vuk
AU - Nikolovski, Branislava G.
PY - 2010
UR - http://fiver.ifvcns.rs/handle/123456789/844
AB - Qualitative and quantitative determination of the Kunitz trypsin inhibitor (KTI) in soybean experimental lines is very important in processes of selecting and breeding of new varieties. The total enzyme activity assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and Lab-on-a-Chip (LoaC) method were used for the determination of the presence and quantity of KTI in 15 soybean experimental lines and varieties. From the total trypsin inhibitor enzyme assay, inhibitor activities were registered in all samples, even in a Kunitz variety that was a negative control. The SDS-PAGE method did not detect the presence of the KTI protein band in seven soybean experimental lines and Kunitz variety, while the LoaC method showed the absence of KTI only in the Kunitz variety sample. Results confirmed the superiority of the LoaC method over other two methods in selectivity and sensitivity when KTI determination is concerned. Relationships between the KTI content obtained by the LoaC method and total trypsin inhibitor enzyme activity were established and statistically confirmed.
PB - Amer Chemical Soc, Washington
T2 - Journal of Agricultural & Food Chemistry
T1 - Advantages of the Lab-on-a-Chip Method in the Determination of the Kunitz Trypsin Inhibitor in Soybean Varieties
EP - 7985
IS - 13
SP - 7980
VL - 58
DO - 10.1021/jf100830m
ER -
@article{
author = "Torbica, Aleksandra and Živančev, Dragan and Nikolić, Zorica and Đorđević, Vuk and Nikolovski, Branislava G.",
year = "2010",
abstract = "Qualitative and quantitative determination of the Kunitz trypsin inhibitor (KTI) in soybean experimental lines is very important in processes of selecting and breeding of new varieties. The total enzyme activity assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and Lab-on-a-Chip (LoaC) method were used for the determination of the presence and quantity of KTI in 15 soybean experimental lines and varieties. From the total trypsin inhibitor enzyme assay, inhibitor activities were registered in all samples, even in a Kunitz variety that was a negative control. The SDS-PAGE method did not detect the presence of the KTI protein band in seven soybean experimental lines and Kunitz variety, while the LoaC method showed the absence of KTI only in the Kunitz variety sample. Results confirmed the superiority of the LoaC method over other two methods in selectivity and sensitivity when KTI determination is concerned. Relationships between the KTI content obtained by the LoaC method and total trypsin inhibitor enzyme activity were established and statistically confirmed.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Agricultural & Food Chemistry",
title = "Advantages of the Lab-on-a-Chip Method in the Determination of the Kunitz Trypsin Inhibitor in Soybean Varieties",
pages = "7985-7980",
number = "13",
volume = "58",
doi = "10.1021/jf100830m"
}
Torbica, A., Živančev, D., Nikolić, Z., Đorđević, V.,& Nikolovski, B. G.. (2010). Advantages of the Lab-on-a-Chip Method in the Determination of the Kunitz Trypsin Inhibitor in Soybean Varieties. in Journal of Agricultural & Food Chemistry
Amer Chemical Soc, Washington., 58(13), 7980-7985.
https://doi.org/10.1021/jf100830m
Torbica A, Živančev D, Nikolić Z, Đorđević V, Nikolovski BG. Advantages of the Lab-on-a-Chip Method in the Determination of the Kunitz Trypsin Inhibitor in Soybean Varieties. in Journal of Agricultural & Food Chemistry. 2010;58(13):7980-7985.
doi:10.1021/jf100830m .
Torbica, Aleksandra, Živančev, Dragan, Nikolić, Zorica, Đorđević, Vuk, Nikolovski, Branislava G., "Advantages of the Lab-on-a-Chip Method in the Determination of the Kunitz Trypsin Inhibitor in Soybean Varieties" in Journal of Agricultural & Food Chemistry, 58, no. 13 (2010):7980-7985,
https://doi.org/10.1021/jf100830m . .
