Phenotypic and molecular evaluation of genetic diversity of rapeseed (Brassica napus L.) genotypes
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Marjanović-Jeromela, Ana
Kondić-Špika, Ankica

Saftić-Panković, Dejana
Marinković, Radovan
Hristov, Nikola
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The genetic diversity and relationships among rapeseed genotypes were evaluated using quantitative analysis and random amplified polymorphic DNA (RAPD) markers. Ten morphological and biochemical traits (plant height, height to the first branch, stem diameter, branch number, leaf number, pod number, seed yield per plant, 1000-seed weight, oil content and protein content) were analyzed in a three-year field experiment with 30 winter rapeseed genotypes. RAPD analysis was performed with 13 primers, chosen according to previous results. Significant genetic variability among the genotypes was obtained at both the morphological and molecular level. Nine out of the thirteen RAPD primers that were used for the analysis enabled the estimation of DNA polymorphism of the genotypes, while the other primers were monomorphic. Dendrograms obtained for morpho-biochemical traits and for the RAPD results were compared. In both cases partial grouping of genotypes based on their geographical origin was est...ablished.
Keywords:
Brassica napus L. / genetic diversity / RAPD / cluster analysisSource:
African Journal of Biotechnology, 2009, 8, 19, 4835-4844Funding / projects:
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FiVeRTY - JOUR AU - Marjanović-Jeromela, Ana AU - Kondić-Špika, Ankica AU - Saftić-Panković, Dejana AU - Marinković, Radovan AU - Hristov, Nikola PY - 2009 UR - http://fiver.ifvcns.rs/handle/123456789/765 AB - The genetic diversity and relationships among rapeseed genotypes were evaluated using quantitative analysis and random amplified polymorphic DNA (RAPD) markers. Ten morphological and biochemical traits (plant height, height to the first branch, stem diameter, branch number, leaf number, pod number, seed yield per plant, 1000-seed weight, oil content and protein content) were analyzed in a three-year field experiment with 30 winter rapeseed genotypes. RAPD analysis was performed with 13 primers, chosen according to previous results. Significant genetic variability among the genotypes was obtained at both the morphological and molecular level. Nine out of the thirteen RAPD primers that were used for the analysis enabled the estimation of DNA polymorphism of the genotypes, while the other primers were monomorphic. Dendrograms obtained for morpho-biochemical traits and for the RAPD results were compared. In both cases partial grouping of genotypes based on their geographical origin was established. T2 - African Journal of Biotechnology T1 - Phenotypic and molecular evaluation of genetic diversity of rapeseed (Brassica napus L.) genotypes EP - 4844 IS - 19 SP - 4835 VL - 8 UR - https://hdl.handle.net/21.15107/rcub_fiver_765 ER -
@article{ author = "Marjanović-Jeromela, Ana and Kondić-Špika, Ankica and Saftić-Panković, Dejana and Marinković, Radovan and Hristov, Nikola", year = "2009", abstract = "The genetic diversity and relationships among rapeseed genotypes were evaluated using quantitative analysis and random amplified polymorphic DNA (RAPD) markers. Ten morphological and biochemical traits (plant height, height to the first branch, stem diameter, branch number, leaf number, pod number, seed yield per plant, 1000-seed weight, oil content and protein content) were analyzed in a three-year field experiment with 30 winter rapeseed genotypes. RAPD analysis was performed with 13 primers, chosen according to previous results. Significant genetic variability among the genotypes was obtained at both the morphological and molecular level. Nine out of the thirteen RAPD primers that were used for the analysis enabled the estimation of DNA polymorphism of the genotypes, while the other primers were monomorphic. Dendrograms obtained for morpho-biochemical traits and for the RAPD results were compared. In both cases partial grouping of genotypes based on their geographical origin was established.", journal = "African Journal of Biotechnology", title = "Phenotypic and molecular evaluation of genetic diversity of rapeseed (Brassica napus L.) genotypes", pages = "4844-4835", number = "19", volume = "8", url = "https://hdl.handle.net/21.15107/rcub_fiver_765" }
Marjanović-Jeromela, A., Kondić-Špika, A., Saftić-Panković, D., Marinković, R.,& Hristov, N.. (2009). Phenotypic and molecular evaluation of genetic diversity of rapeseed (Brassica napus L.) genotypes. in African Journal of Biotechnology, 8(19), 4835-4844. https://hdl.handle.net/21.15107/rcub_fiver_765
Marjanović-Jeromela A, Kondić-Špika A, Saftić-Panković D, Marinković R, Hristov N. Phenotypic and molecular evaluation of genetic diversity of rapeseed (Brassica napus L.) genotypes. in African Journal of Biotechnology. 2009;8(19):4835-4844. https://hdl.handle.net/21.15107/rcub_fiver_765 .
Marjanović-Jeromela, Ana, Kondić-Špika, Ankica, Saftić-Panković, Dejana, Marinković, Radovan, Hristov, Nikola, "Phenotypic and molecular evaluation of genetic diversity of rapeseed (Brassica napus L.) genotypes" in African Journal of Biotechnology, 8, no. 19 (2009):4835-4844, https://hdl.handle.net/21.15107/rcub_fiver_765 .