The application of the near infrared spectroscopy in the determination of the protein and oil content in soybean seeds

Abstract

The possibility of a fast and non-destructive estimation of the chemical composition of the soybean seed is one of the most important factors in soybean breeding, soybean seed production, as well as, soybean processing. The near infrared (NIR) spectrum is a small part of the light spectrum and pertains wave lengths between 700 and 2500 nm. These parts of the spectrum contain a large number of absorptions bands, overtones and combined bands, which originate from different organic molecules and provide quantitative and qualitative chemical analyses of the soybean seed. The application of multivariate statistical methods provide quantitative chemical analyses of different classes of organic molecules in the soybean seed. Calibration equations were developed from 220 soybean seed samples. Close relationships between standard analytical methods and NIR values were estimated. The coefficients of determination for the protein and oil contents were 0.97 and 0.93, respectively. The NIR spectroscopy is a rapid, robust and reliable method for quantitative analyses of the protein and oil content in the intact soybean seed.

Mogućnost brzog i nedestruktivnog određivanja hemijskog sastav semena soje predstavlja bitan faktor u oplemenjivanju i semenarstvu soje, kao i u prerađivačkoj industriji. Bliski infracrveni (NIR) deo spektra, predstavlja samo mali deo spektra svetlosti i odnosi se na talasne dužine od 700 do 2,500 nm. U ovom delu spektra nalazi se veliki broj apsorpcionih traka i kombinovanih traka, koji potiču od različitih organskih molekula i omogućavaju kvantitativnu i kvalitativnu hemijsku analizu semena soje. Uz primenu multivariacionih statističkih metoda moguća je kvantitativna analiza različitih klasa organskih molekula u semenu soje. Jednačine kalibracionih krivih konstruisane su na osnovu 220 uzoraka semena soje. Utvrđene su bliske vrednosti dobijene merenjem standardnim laboratorijskim tehnikama i NIR tehnikom. Koeficijent determinacije za sadržaj proteina iznosio je 0,97 dok je za sadržaj ulja bio 0,93 NIR spektroskopija je brza, robusna i pouzdana tehnika pomoću koje je moguće kvantitativno određivanje saržaja proteina i ulja u intaktnom semenu soju.