Elaboration of methods for detection of Pseudomonas savastanoi pv. phaseolicola on bean seeds
Razrada metoda za dokazivanje Pseudomonas savastanoi pv. phaseolicola na semenu pasulja
Апстракт
Pseudomonas savastanoi pv. phaseolicola detection on artificially inoculated bean seeds was investigated. The method of the International Seed Federation - ISF (2006) was used. It includes bacteria extraction from seeds, isolation on semiselective media and checking the pathogenicity of investigated isolates. For verification of results, quick new methods of investigation were used (ELISA test and PCR). The results show that semiselective media MT (Milk Tween Agar) and MSP (Modified Sucrose Peptone Agar) can be appropriate for isolation of this bacterium. Pathogenicity of the investigated isolates was confirmed on cotyledon leaves of bean. ELISA test and PCR confirmed that all investigated isolates and reisolates belong to the bacterium P.s.pv. phaseolicola.
U radu je ispitivana mogućnost utvrđivanja prisustva bakterije Pseudomonas savastanoi pv. phaseolicola sa veštački inokulisanog semena pasulja. Korišćena je metoda International Seed Federation (ISF), koja uključuje ekstrakciju bakterija iz semena, izolaciju na poluselektivne podloge i proveru patogenosti dobijenih izolata. U cilju potvrde rezultata korišćene su brze savremene metode identifikacije (ELISA test i PCR). Rezultati dobijeni tokom rada pokazuju da su poluselektivne podloge MT (Milk Tween Agar) i MSP (Modified Sucrose Peptone Agar) pogodne za izolaciju ove bakterije. Patogenost odabranih izolata dokazana je na kotiledonim listovima pasulja. Primenom ELISA testova i PCR, potvrđeno je da svi ispitivani izolati i reizolati pripadaju bakteriji P.s.pv. phaseolicola.
Кључне речи:
Pseudomonas savastanoi pv. phaseolicola / bean / seed / artificial inoculation / ELISA / PCR / Pseudomonas savastanoi pv. phaseolicola / pasulj / seme / veštačka inokulacija / ELISA / PCRИзвор:
Pesticidi i fitomedicina, 2008, 23, 2, 81-88Издавач:
- Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
Колекције
Институција/група
FiVeRTY - JOUR AU - Balaž, Jelica AU - Popović, Tatjana AU - Vasić, Mirjana AU - Nikolić, Zorica PY - 2008 UR - http://fiver.ifvcns.rs/handle/123456789/582 AB - Pseudomonas savastanoi pv. phaseolicola detection on artificially inoculated bean seeds was investigated. The method of the International Seed Federation - ISF (2006) was used. It includes bacteria extraction from seeds, isolation on semiselective media and checking the pathogenicity of investigated isolates. For verification of results, quick new methods of investigation were used (ELISA test and PCR). The results show that semiselective media MT (Milk Tween Agar) and MSP (Modified Sucrose Peptone Agar) can be appropriate for isolation of this bacterium. Pathogenicity of the investigated isolates was confirmed on cotyledon leaves of bean. ELISA test and PCR confirmed that all investigated isolates and reisolates belong to the bacterium P.s.pv. phaseolicola. AB - U radu je ispitivana mogućnost utvrđivanja prisustva bakterije Pseudomonas savastanoi pv. phaseolicola sa veštački inokulisanog semena pasulja. Korišćena je metoda International Seed Federation (ISF), koja uključuje ekstrakciju bakterija iz semena, izolaciju na poluselektivne podloge i proveru patogenosti dobijenih izolata. U cilju potvrde rezultata korišćene su brze savremene metode identifikacije (ELISA test i PCR). Rezultati dobijeni tokom rada pokazuju da su poluselektivne podloge MT (Milk Tween Agar) i MSP (Modified Sucrose Peptone Agar) pogodne za izolaciju ove bakterije. Patogenost odabranih izolata dokazana je na kotiledonim listovima pasulja. Primenom ELISA testova i PCR, potvrđeno je da svi ispitivani izolati i reizolati pripadaju bakteriji P.s.pv. phaseolicola. PB - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd T2 - Pesticidi i fitomedicina T1 - Elaboration of methods for detection of Pseudomonas savastanoi pv. phaseolicola on bean seeds T1 - Razrada metoda za dokazivanje Pseudomonas savastanoi pv. phaseolicola na semenu pasulja EP - 88 IS - 2 SP - 81 VL - 23 DO - 10.2298/PIF0802081B ER -
@article{ author = "Balaž, Jelica and Popović, Tatjana and Vasić, Mirjana and Nikolić, Zorica", year = "2008", abstract = "Pseudomonas savastanoi pv. phaseolicola detection on artificially inoculated bean seeds was investigated. The method of the International Seed Federation - ISF (2006) was used. It includes bacteria extraction from seeds, isolation on semiselective media and checking the pathogenicity of investigated isolates. For verification of results, quick new methods of investigation were used (ELISA test and PCR). The results show that semiselective media MT (Milk Tween Agar) and MSP (Modified Sucrose Peptone Agar) can be appropriate for isolation of this bacterium. Pathogenicity of the investigated isolates was confirmed on cotyledon leaves of bean. ELISA test and PCR confirmed that all investigated isolates and reisolates belong to the bacterium P.s.pv. phaseolicola., U radu je ispitivana mogućnost utvrđivanja prisustva bakterije Pseudomonas savastanoi pv. phaseolicola sa veštački inokulisanog semena pasulja. Korišćena je metoda International Seed Federation (ISF), koja uključuje ekstrakciju bakterija iz semena, izolaciju na poluselektivne podloge i proveru patogenosti dobijenih izolata. U cilju potvrde rezultata korišćene su brze savremene metode identifikacije (ELISA test i PCR). Rezultati dobijeni tokom rada pokazuju da su poluselektivne podloge MT (Milk Tween Agar) i MSP (Modified Sucrose Peptone Agar) pogodne za izolaciju ove bakterije. Patogenost odabranih izolata dokazana je na kotiledonim listovima pasulja. Primenom ELISA testova i PCR, potvrđeno je da svi ispitivani izolati i reizolati pripadaju bakteriji P.s.pv. phaseolicola.", publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd", journal = "Pesticidi i fitomedicina", title = "Elaboration of methods for detection of Pseudomonas savastanoi pv. phaseolicola on bean seeds, Razrada metoda za dokazivanje Pseudomonas savastanoi pv. phaseolicola na semenu pasulja", pages = "88-81", number = "2", volume = "23", doi = "10.2298/PIF0802081B" }
Balaž, J., Popović, T., Vasić, M.,& Nikolić, Z.. (2008). Elaboration of methods for detection of Pseudomonas savastanoi pv. phaseolicola on bean seeds. in Pesticidi i fitomedicina Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 23(2), 81-88. https://doi.org/10.2298/PIF0802081B
Balaž J, Popović T, Vasić M, Nikolić Z. Elaboration of methods for detection of Pseudomonas savastanoi pv. phaseolicola on bean seeds. in Pesticidi i fitomedicina. 2008;23(2):81-88. doi:10.2298/PIF0802081B .
Balaž, Jelica, Popović, Tatjana, Vasić, Mirjana, Nikolić, Zorica, "Elaboration of methods for detection of Pseudomonas savastanoi pv. phaseolicola on bean seeds" in Pesticidi i fitomedicina, 23, no. 2 (2008):81-88, https://doi.org/10.2298/PIF0802081B . .