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High production of wheat double haploids via anther culture
Visoka produkcija dvostrukih haploida pšenice korišćenjem kulture antera
dc.creator | Kondić-Špika, Ankica | |
dc.creator | Kobiljski, Borislav | |
dc.creator | Hristov, Nikola | |
dc.date.accessioned | 2021-04-26T18:16:37Z | |
dc.date.available | 2021-04-26T18:16:37Z | |
dc.date.issued | 2007 | |
dc.identifier.issn | 0534-0012 | |
dc.identifier.uri | http://fiver.ifvcns.rs/handle/123456789/481 | |
dc.description.abstract | Androgenous and regeneration abilities of 14 randomly selected F1 hybrids of wheat (Triticum aestivum L.) were analyzed. Anthers were grown in vitro on a modified Potato-2 inductive medium. The hybrid NS111-95/Ana had the highest average values for androgenous capacity (33%) and callus yield (119%), while the hybrid NS 92-250/Tiha had the lowest values for these traits (9 and 21%, respectively). Seven genotypes (50%) had a frequency of green plants relative to the number of isolated anthers of over 10%, with the highest frequency of 21.3% (NS111-95/Sremica). This hybrid produced 12.8 doubled haploid (DH) lines per spike used for isolation. In the other genotypes, the number of produced DH lines per spike ranged from 1 (30Sc.Smoc.88-89/Hays-2) to 11.2 (NS111-95/Ana). As half of the randomly selected genotypes exhibited high green plant regeneration ability and a high production of DH lines per spike, it can be concluded that in vitro anther culture can be successfully used in breeding programs for rapid production of homozygous wheat lines. | en |
dc.description.abstract | Analizirana je androgena i regeneraciona sposobnost 14 slučajno odabranih F1 hibrida pšenice (Triticum aestivum L.). Antere su gajene u in vitro uslovima na modifikovanoj Potatao-2 podlozi. Hibrid NS111-95/Ana imao je najviše prosečne vrednosti za androgeni kapacitet (33%) i prinos kalusa (119%), dok je hibrid NS 92-250/Tiha imao najniže vrednosti za ove osobine (9% i 21%). Sedam genotipova (50%) imalo je frekvenciju zelenih biljaka u odnosu na broj izolovanih antera preko 10%, sa najvišom vrednošću od 21.3% (NS1195/Sremica). Kod istog hibrida (NS11-95/Sremica) proizvedeno je 12.8 linija dvostrukih haploida (DH) po klasu, korišćenom za izolaciju. Kod drugih genotipova, broj proizvedenih DH linija po klasu varirao je od 1 (30-Sc.Smoc.8889/Hays-2) do 11.2 (NS111-95/Ana). S obzirom na to da je polovina slučajno odabranih genotipova ispoljila visoku sposobnost za regeneraciju zelenih biljaka i visoku produkciju DH linija po klasu, in vitro kultura antera može se uspešno koristiti u oplemenjivačkim programima za brzu proizvodnju homozigotnih linija pšenice. | sr |
dc.publisher | Društvo genetičara Srbije, Beograd | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Genetika-Belgrade | |
dc.subject | anther culture | en |
dc.subject | doubled haploid | en |
dc.subject | wheat | en |
dc.title | High production of wheat double haploids via anther culture | en |
dc.title | Visoka produkcija dvostrukih haploida pšenice korišćenjem kulture antera | sr |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dc.citation.epage | 411 | |
dc.citation.issue | 3 | |
dc.citation.other | 39(3): 405-411 | |
dc.citation.spage | 405 | |
dc.citation.volume | 39 | |
dc.identifier.doi | 10.2298/GENSR0703405K | |
dc.identifier.fulltext | http://fiver.ifvcns.rs/bitstream/id/1336/478.pdf | |
dc.type.version | publishedVersion |