Biochemical response of sunflower inbred lines inoculated with Macrophomina phaseolina
Authors
Ćuk, NemanjaKiprovski, Biljana
Cvejić, Sandra
Dedić, Boško
Babec, Brankica
Krstić, Miloš
Jocić, Siniša
Miklič, Vladimir
Jocković, Jelena
Jocković, Milan
Mladenov, Velimir
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Macrophomina phaseolina, the causative agent of charcoal rot, affects a wide array of plant hosts, including sunflower. This disease thrives in warm, arid conditions, leading to symptoms such as the wilting, drying, and premature ripening of sunflower plants. This study aims to explore the biochemical responses of 15 inbred lines, each exhibiting varying levels of resistance, to uncover potential correlations between resistance levels and biochemical reactions in sunflower inbred lines. The investigation focused on: total protein content (TPC), lipid peroxidation intensity as a marker of membrane integrity (LP), reduced glutathione (GSH), superoxide-dismutase activity (SOD), and total phenolic content as antioxidant compounds (TP). These parameters were assessed 10 days following the laboratory inoculation of inbred lines with the pathogen and compared with disease severity. Correlations between resistance levels and the results of these five assays were analyzed in conjunction with di...sease severity observed in the inbred lines. Remarkably, after the 10-day assessment period, only the total phenolic content showed a significant positive correlation with the resistance of inbred lines (r=0.712; p<0.05). This finding identified inbred lines PB 21 and L 1 as the most resilient among the tested varieties. It was observed that different inbred lines exhibit distinct responses to M. phaseolina. However, in the majority of cases, an increase in total phenolic content was noted in sunflower plants following inoculation. This suggests a potential defensive mechanism triggered by the pathogen. Further studies can analyze more precisely into the molecular intricacies of sunflower resistance to charcoal rot and validate these findings across broader genetic backgrounds.
Keywords:
charcoal rot / Macrophomina phaseolina / disease severity / resistance / total phenolic content / sunflower / inbred lines / diseasesSource:
Book of Abstracts, 4th EPI-CATCH Conference Epigenetic Mechanisms of Crop Adaptation to Climate Change, Novi Sad, 4-6 June 2024, 2024Funding / projects:
- SmartSun - Creating climate smart sunflower for future challenges (RS-ScienceFundRS-Ideje-7732457)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200032 (Institute of Field and Vegetable Crops, Novi Sad) (RS-MESTD-inst-2020-200032)
- CROPINNO - Stepping up scientific excellence and innovation capacity for climate-resilient crop improvement and production (EU-HE-101059784)
- CROPINNO - Stepping up scientific excellence and innovation capacity for climate-resilient crop improvement and production (EU-HE-101059784)
- Climate Crops - Centre of Excellence for Innovations in Breeding of Climate-Resilient Crops, Institute of Field and Vegetable Crops
Note:
- This is a poster presented at the conference. The published abstract is available here http://fiver.ifvcns.rs/handle/123456789/4697
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FiVeRTY - CONF AU - Ćuk, Nemanja AU - Kiprovski, Biljana AU - Cvejić, Sandra AU - Dedić, Boško AU - Babec, Brankica AU - Krstić, Miloš AU - Jocić, Siniša AU - Miklič, Vladimir AU - Jocković, Jelena AU - Jocković, Milan AU - Mladenov, Velimir PY - 2024 UR - http://fiver.ifvcns.rs/handle/123456789/4698 AB - Macrophomina phaseolina, the causative agent of charcoal rot, affects a wide array of plant hosts, including sunflower. This disease thrives in warm, arid conditions, leading to symptoms such as the wilting, drying, and premature ripening of sunflower plants. This study aims to explore the biochemical responses of 15 inbred lines, each exhibiting varying levels of resistance, to uncover potential correlations between resistance levels and biochemical reactions in sunflower inbred lines. The investigation focused on: total protein content (TPC), lipid peroxidation intensity as a marker of membrane integrity (LP), reduced glutathione (GSH), superoxide-dismutase activity (SOD), and total phenolic content as antioxidant compounds (TP). These parameters were assessed 10 days following the laboratory inoculation of inbred lines with the pathogen and compared with disease severity. Correlations between resistance levels and the results of these five assays were analyzed in conjunction with disease severity observed in the inbred lines. Remarkably, after the 10-day assessment period, only the total phenolic content showed a significant positive correlation with the resistance of inbred lines (r=0.712; p<0.05). This finding identified inbred lines PB 21 and L 1 as the most resilient among the tested varieties. It was observed that different inbred lines exhibit distinct responses to M. phaseolina. However, in the majority of cases, an increase in total phenolic content was noted in sunflower plants following inoculation. This suggests a potential defensive mechanism triggered by the pathogen. Further studies can analyze more precisely into the molecular intricacies of sunflower resistance to charcoal rot and validate these findings across broader genetic backgrounds. C3 - Book of Abstracts, 4th EPI-CATCH Conference Epigenetic Mechanisms of Crop Adaptation to Climate Change, Novi Sad, 4-6 June 2024 T1 - Biochemical response of sunflower inbred lines inoculated with Macrophomina phaseolina UR - https://hdl.handle.net/21.15107/rcub_fiver_4698 ER -
@conference{ author = "Ćuk, Nemanja and Kiprovski, Biljana and Cvejić, Sandra and Dedić, Boško and Babec, Brankica and Krstić, Miloš and Jocić, Siniša and Miklič, Vladimir and Jocković, Jelena and Jocković, Milan and Mladenov, Velimir", year = "2024", abstract = "Macrophomina phaseolina, the causative agent of charcoal rot, affects a wide array of plant hosts, including sunflower. This disease thrives in warm, arid conditions, leading to symptoms such as the wilting, drying, and premature ripening of sunflower plants. This study aims to explore the biochemical responses of 15 inbred lines, each exhibiting varying levels of resistance, to uncover potential correlations between resistance levels and biochemical reactions in sunflower inbred lines. The investigation focused on: total protein content (TPC), lipid peroxidation intensity as a marker of membrane integrity (LP), reduced glutathione (GSH), superoxide-dismutase activity (SOD), and total phenolic content as antioxidant compounds (TP). These parameters were assessed 10 days following the laboratory inoculation of inbred lines with the pathogen and compared with disease severity. Correlations between resistance levels and the results of these five assays were analyzed in conjunction with disease severity observed in the inbred lines. Remarkably, after the 10-day assessment period, only the total phenolic content showed a significant positive correlation with the resistance of inbred lines (r=0.712; p<0.05). This finding identified inbred lines PB 21 and L 1 as the most resilient among the tested varieties. It was observed that different inbred lines exhibit distinct responses to M. phaseolina. However, in the majority of cases, an increase in total phenolic content was noted in sunflower plants following inoculation. This suggests a potential defensive mechanism triggered by the pathogen. Further studies can analyze more precisely into the molecular intricacies of sunflower resistance to charcoal rot and validate these findings across broader genetic backgrounds.", journal = "Book of Abstracts, 4th EPI-CATCH Conference Epigenetic Mechanisms of Crop Adaptation to Climate Change, Novi Sad, 4-6 June 2024", title = "Biochemical response of sunflower inbred lines inoculated with Macrophomina phaseolina", url = "https://hdl.handle.net/21.15107/rcub_fiver_4698" }
Ćuk, N., Kiprovski, B., Cvejić, S., Dedić, B., Babec, B., Krstić, M., Jocić, S., Miklič, V., Jocković, J., Jocković, M.,& Mladenov, V.. (2024). Biochemical response of sunflower inbred lines inoculated with Macrophomina phaseolina. in Book of Abstracts, 4th EPI-CATCH Conference Epigenetic Mechanisms of Crop Adaptation to Climate Change, Novi Sad, 4-6 June 2024. https://hdl.handle.net/21.15107/rcub_fiver_4698
Ćuk N, Kiprovski B, Cvejić S, Dedić B, Babec B, Krstić M, Jocić S, Miklič V, Jocković J, Jocković M, Mladenov V. Biochemical response of sunflower inbred lines inoculated with Macrophomina phaseolina. in Book of Abstracts, 4th EPI-CATCH Conference Epigenetic Mechanisms of Crop Adaptation to Climate Change, Novi Sad, 4-6 June 2024. 2024;. https://hdl.handle.net/21.15107/rcub_fiver_4698 .
Ćuk, Nemanja, Kiprovski, Biljana, Cvejić, Sandra, Dedić, Boško, Babec, Brankica, Krstić, Miloš, Jocić, Siniša, Miklič, Vladimir, Jocković, Jelena, Jocković, Milan, Mladenov, Velimir, "Biochemical response of sunflower inbred lines inoculated with Macrophomina phaseolina" in Book of Abstracts, 4th EPI-CATCH Conference Epigenetic Mechanisms of Crop Adaptation to Climate Change, Novi Sad, 4-6 June 2024 (2024), https://hdl.handle.net/21.15107/rcub_fiver_4698 .