Bio-Priming with Bacillus Isolates Suppresses Seed Infection and Improves the Germination of Garden Peas in the Presence of Fusarium Strains
Authors
Miljaković, DraganaMarinković, Jelena
Tamindžić, Gordana
Milošević, Dragana
Ignjatov, Maja
Karačić, Vasiljka
Jakšić, Snežana
Article (Published version)
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Seed infection caused by Fusarium spp. is one of the major threats to the seed quality and
yield of agricultural crops, including garden peas. The use of Bacillus spp. with multiple antagonistic
and plant growth-promoting (PGP) abilities represents a potential disease control strategy. This
study was performed to evaluate the biocontrol potential of new Bacillus spp. rhizosphere isolates
against two Fusarium strains affecting garden peas. Six Bacillus isolates identified by 16S rDNA sequencing as B. velezensis (B42), B. subtilis (B43), B. mojavensis (B44, B46), B. amyloliquefaciens (B50),
and B. halotolerans (B66) showed the highest in vitro inhibition of F. proliferatum PS1 and F. equiseti
PS18 growth (over 40%). The selected Bacillus isolates possessed biosynthetic genes for endoglucanase (B42, B43, B50), surfactin (B43, B44, B46), fengycin (B44, B46), bacillomycin D (B42, B50), and
iturin (B42), and were able to produce indole-3-acetic acid (IAA), siderophores, and cellulase.... Two
isolates, B. subtilis B43 and B. amyloliquefaciens B50, had the highest effect on final germination, shoot
length, root length, shoot dry weight, root dry weight, and seedling vigor index of garden peas as
compared to the control. Their individual or combined application reduced seed infection and increased seed germination in the presence of F. proliferatum PS1 and F. equiseti PS18, both after seed
inoculation and seed bio-priming. The most promising results were obtained in the cases of the
bacterial consortium, seed bio-priming, and the more pathogenic strain PS18. The novel Bacillus
isolates may be potential biocontrol agents intended for the management of Fusarium seed-borne
diseases.
Keywords:
Bacillus subtilis / Bacillus amyloliquefaciens / Fusarium biocontrol / Pisum sativum L. / seed bio-primingSource:
Journal of Fungi, 2024, 10, 5, 358-Publisher:
- Basel : MDPI
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200032 (Institute of Field and Vegetable Crops, Novi Sad) (RS-MESTD-inst-2020-200032)
- Bilateral project Belarus - Serbia, 337-00-00230/2022-09/11, Metaboličko profilisanje i farmaceutski potencijal genotipova lucerke, deteline i soje u Belorusiji i Srbiji: razvoj metodološke platforme i digitalnih biblioteka metaboloma za naučnu primenu
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FiVeRTY - JOUR AU - Miljaković, Dragana AU - Marinković, Jelena AU - Tamindžić, Gordana AU - Milošević, Dragana AU - Ignjatov, Maja AU - Karačić, Vasiljka AU - Jakšić, Snežana PY - 2024 UR - http://fiver.ifvcns.rs/handle/123456789/4641 AB - Seed infection caused by Fusarium spp. is one of the major threats to the seed quality and yield of agricultural crops, including garden peas. The use of Bacillus spp. with multiple antagonistic and plant growth-promoting (PGP) abilities represents a potential disease control strategy. This study was performed to evaluate the biocontrol potential of new Bacillus spp. rhizosphere isolates against two Fusarium strains affecting garden peas. Six Bacillus isolates identified by 16S rDNA sequencing as B. velezensis (B42), B. subtilis (B43), B. mojavensis (B44, B46), B. amyloliquefaciens (B50), and B. halotolerans (B66) showed the highest in vitro inhibition of F. proliferatum PS1 and F. equiseti PS18 growth (over 40%). The selected Bacillus isolates possessed biosynthetic genes for endoglucanase (B42, B43, B50), surfactin (B43, B44, B46), fengycin (B44, B46), bacillomycin D (B42, B50), and iturin (B42), and were able to produce indole-3-acetic acid (IAA), siderophores, and cellulase. Two isolates, B. subtilis B43 and B. amyloliquefaciens B50, had the highest effect on final germination, shoot length, root length, shoot dry weight, root dry weight, and seedling vigor index of garden peas as compared to the control. Their individual or combined application reduced seed infection and increased seed germination in the presence of F. proliferatum PS1 and F. equiseti PS18, both after seed inoculation and seed bio-priming. The most promising results were obtained in the cases of the bacterial consortium, seed bio-priming, and the more pathogenic strain PS18. The novel Bacillus isolates may be potential biocontrol agents intended for the management of Fusarium seed-borne diseases. PB - Basel : MDPI T2 - Journal of Fungi T1 - Bio-Priming with Bacillus Isolates Suppresses Seed Infection and Improves the Germination of Garden Peas in the Presence of Fusarium Strains IS - 5 SP - 358 VL - 10 DO - 10.3390/jof10050358 ER -
@article{ author = "Miljaković, Dragana and Marinković, Jelena and Tamindžić, Gordana and Milošević, Dragana and Ignjatov, Maja and Karačić, Vasiljka and Jakšić, Snežana", year = "2024", abstract = "Seed infection caused by Fusarium spp. is one of the major threats to the seed quality and yield of agricultural crops, including garden peas. The use of Bacillus spp. with multiple antagonistic and plant growth-promoting (PGP) abilities represents a potential disease control strategy. This study was performed to evaluate the biocontrol potential of new Bacillus spp. rhizosphere isolates against two Fusarium strains affecting garden peas. Six Bacillus isolates identified by 16S rDNA sequencing as B. velezensis (B42), B. subtilis (B43), B. mojavensis (B44, B46), B. amyloliquefaciens (B50), and B. halotolerans (B66) showed the highest in vitro inhibition of F. proliferatum PS1 and F. equiseti PS18 growth (over 40%). The selected Bacillus isolates possessed biosynthetic genes for endoglucanase (B42, B43, B50), surfactin (B43, B44, B46), fengycin (B44, B46), bacillomycin D (B42, B50), and iturin (B42), and were able to produce indole-3-acetic acid (IAA), siderophores, and cellulase. Two isolates, B. subtilis B43 and B. amyloliquefaciens B50, had the highest effect on final germination, shoot length, root length, shoot dry weight, root dry weight, and seedling vigor index of garden peas as compared to the control. Their individual or combined application reduced seed infection and increased seed germination in the presence of F. proliferatum PS1 and F. equiseti PS18, both after seed inoculation and seed bio-priming. The most promising results were obtained in the cases of the bacterial consortium, seed bio-priming, and the more pathogenic strain PS18. The novel Bacillus isolates may be potential biocontrol agents intended for the management of Fusarium seed-borne diseases.", publisher = "Basel : MDPI", journal = "Journal of Fungi", title = "Bio-Priming with Bacillus Isolates Suppresses Seed Infection and Improves the Germination of Garden Peas in the Presence of Fusarium Strains", number = "5", pages = "358", volume = "10", doi = "10.3390/jof10050358" }
Miljaković, D., Marinković, J., Tamindžić, G., Milošević, D., Ignjatov, M., Karačić, V.,& Jakšić, S.. (2024). Bio-Priming with Bacillus Isolates Suppresses Seed Infection and Improves the Germination of Garden Peas in the Presence of Fusarium Strains. in Journal of Fungi Basel : MDPI., 10(5), 358. https://doi.org/10.3390/jof10050358
Miljaković D, Marinković J, Tamindžić G, Milošević D, Ignjatov M, Karačić V, Jakšić S. Bio-Priming with Bacillus Isolates Suppresses Seed Infection and Improves the Germination of Garden Peas in the Presence of Fusarium Strains. in Journal of Fungi. 2024;10(5):358. doi:10.3390/jof10050358 .
Miljaković, Dragana, Marinković, Jelena, Tamindžić, Gordana, Milošević, Dragana, Ignjatov, Maja, Karačić, Vasiljka, Jakšić, Snežana, "Bio-Priming with Bacillus Isolates Suppresses Seed Infection and Improves the Germination of Garden Peas in the Presence of Fusarium Strains" in Journal of Fungi, 10, no. 5 (2024):358, https://doi.org/10.3390/jof10050358 . .