Screening of Bacillus spp. as potential biocontrol agents against sunflower pathogens
Ispitivanje Bacillus spp. kao potencijalnih agenasa biokontrole patogena suncokreta
2022
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Authors
Miljaković, DraganaMilošević, Dragana
Ignjatov, Maja
Marinković, Jelena
Tamindžić, Gordana
Tintor, Branislava
Nikolić, Zorica
Article (Published version)
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Bacillus spp. are well known to protect plants from seed or soil-borne
pathogens by the synthesis of various metabolites with antimicrobial activity, such as hydrolytic enzymes and antibiotics. This study aimed to select the most effective Bacillus spp.
from a group of ten antagonistic strains by antifungal activity assay. Bacillus strains were
primarily isolated from the soil and identified as B. safensis, B. pumilus and B. subtilis by
16S rDNA sequencing. The four analyzed fungi: Macrophomina phaseolina, Alternaria
alternata, Cladosporium cladosporoides, and Sclerotinia sclerotiorum, were obtained from
sunflower seeds and identified using PCR analysis and primers specific for ITS region. The
antifungal activity of bacterial strains was examined in a dual plate assay. Bacillus spp.
demonstrated the highest antagonism against S. sclerotiorum, followed by C. cladosporoides,
M. phaseolina, and A. alternata, with an average percentage of growth inhibition (PGI) of 77%,
70%, 64% ...and 59%, respectively. Overall, Bacillus spp. included in this study demonstrated
a rather strong biocontrol potential, although the effect of particular strain varied depending
on the tested fungi. The highest antagonistic effect toward M. phaseolina and A. alternata
was exhibited by B. safensis B2 and B. pumilus B3. B. pumilus B11 and B. subtilis B32 were
the most efficient against C. cladosporoides, whereas B. pumilus B3 and B. subtilis B7 had
the highest antifungal activity against S. sclerotiorum. Findings point to the fact that the
most effective Bacillus spp. could be used as potential biocontrol agents for improving plant
health and productivity.
Bacillus spp. suzbijaju fitopatogene sintezom različitih metabolita sa antimikrobnim delovanjem kao što su litički enzimi i antibiotici. Cilj ovih istraživanja bila je selekcija najefektivnijih Bacillus spp. iz grupe antagonističkih sojeva ispitivanjem njihove antifungalne aktivnosti. Bacillus sojevi su prvobitno izolovani iz zemljišta i identifikovani kao B. safensis, B. pumilus i B. subtilis sekvenciranjem 16S rDNA. Analizirane gljive, Macrophomina phaseolina, Alternaria alternata, Cladosporium cladosporoides i Sclerotinia sclerotiorum, izolovane su sa semena suncokreta i identifikovane PCR analizom uz primenu prajmera specifičnih za ITS region. Antifungalna aktivnost bakterija prema fitopatogenim gljivama ispitana je metodom dvojne kultivacije. Bacillus spp. ispoljili su najveći antagonizam prema S. sclerotiorum, zatim C. cladosporoides, M. phaseolina i A. alternata, sa prosečnim procentom inhibicije rasta 77%, 70%, 64% i 59%. Ispitivani sojevi Bacillus spp. pokazali su veoma jak bi...okontrolni potencijal, iako je efekat određenog soja varirao u zavisnosti od ispitivane gljive. Najveći antagonistički efekat prema M. phaseolina i A. alternata imali su sojevi B. safensis B2 i B. pumilus B3. Najefektivniji sojevi protiv C. cladosporoides bili su B. pumilus B11 i B. subtilis B32, dok je najveća antifungalna aktivnost prema S. sclerotiorum utvrđen primenom sojeva B. pumilus B3 i B. subtilis B7. Rezultati ovih istraživanja pokazuju da se najefektivniji sojevi Bacillus spp. mogu koristiti kao potencijalni agensi biokontrole za poboljšanje zdravlja i produktivnosti biljaka.
Keywords:
Alternaria / antifungal activity / Bacillus / biocontrol / Cladosporium / Macrophomina / Sclerotinia / sunflower / antifungalna aktivnost / biokontrola / suncokretSource:
Matica Srpska Journal for Natural Sciences, 2022, 143, 65-72Publisher:
- Novi Sad : Matica srpska
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200032 (Institute of Field and Vegetable Crops, Novi Sad) (RS-MESTD-inst-2020-200032)
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FiVeRTY - JOUR AU - Miljaković, Dragana AU - Milošević, Dragana AU - Ignjatov, Maja AU - Marinković, Jelena AU - Tamindžić, Gordana AU - Tintor, Branislava AU - Nikolić, Zorica PY - 2022 UR - http://fiver.ifvcns.rs/handle/123456789/3250 AB - Bacillus spp. are well known to protect plants from seed or soil-borne pathogens by the synthesis of various metabolites with antimicrobial activity, such as hydrolytic enzymes and antibiotics. This study aimed to select the most effective Bacillus spp. from a group of ten antagonistic strains by antifungal activity assay. Bacillus strains were primarily isolated from the soil and identified as B. safensis, B. pumilus and B. subtilis by 16S rDNA sequencing. The four analyzed fungi: Macrophomina phaseolina, Alternaria alternata, Cladosporium cladosporoides, and Sclerotinia sclerotiorum, were obtained from sunflower seeds and identified using PCR analysis and primers specific for ITS region. The antifungal activity of bacterial strains was examined in a dual plate assay. Bacillus spp. demonstrated the highest antagonism against S. sclerotiorum, followed by C. cladosporoides, M. phaseolina, and A. alternata, with an average percentage of growth inhibition (PGI) of 77%, 70%, 64% and 59%, respectively. Overall, Bacillus spp. included in this study demonstrated a rather strong biocontrol potential, although the effect of particular strain varied depending on the tested fungi. The highest antagonistic effect toward M. phaseolina and A. alternata was exhibited by B. safensis B2 and B. pumilus B3. B. pumilus B11 and B. subtilis B32 were the most efficient against C. cladosporoides, whereas B. pumilus B3 and B. subtilis B7 had the highest antifungal activity against S. sclerotiorum. Findings point to the fact that the most effective Bacillus spp. could be used as potential biocontrol agents for improving plant health and productivity. AB - Bacillus spp. suzbijaju fitopatogene sintezom različitih metabolita sa antimikrobnim delovanjem kao što su litički enzimi i antibiotici. Cilj ovih istraživanja bila je selekcija najefektivnijih Bacillus spp. iz grupe antagonističkih sojeva ispitivanjem njihove antifungalne aktivnosti. Bacillus sojevi su prvobitno izolovani iz zemljišta i identifikovani kao B. safensis, B. pumilus i B. subtilis sekvenciranjem 16S rDNA. Analizirane gljive, Macrophomina phaseolina, Alternaria alternata, Cladosporium cladosporoides i Sclerotinia sclerotiorum, izolovane su sa semena suncokreta i identifikovane PCR analizom uz primenu prajmera specifičnih za ITS region. Antifungalna aktivnost bakterija prema fitopatogenim gljivama ispitana je metodom dvojne kultivacije. Bacillus spp. ispoljili su najveći antagonizam prema S. sclerotiorum, zatim C. cladosporoides, M. phaseolina i A. alternata, sa prosečnim procentom inhibicije rasta 77%, 70%, 64% i 59%. Ispitivani sojevi Bacillus spp. pokazali su veoma jak biokontrolni potencijal, iako je efekat određenog soja varirao u zavisnosti od ispitivane gljive. Najveći antagonistički efekat prema M. phaseolina i A. alternata imali su sojevi B. safensis B2 i B. pumilus B3. Najefektivniji sojevi protiv C. cladosporoides bili su B. pumilus B11 i B. subtilis B32, dok je najveća antifungalna aktivnost prema S. sclerotiorum utvrđen primenom sojeva B. pumilus B3 i B. subtilis B7. Rezultati ovih istraživanja pokazuju da se najefektivniji sojevi Bacillus spp. mogu koristiti kao potencijalni agensi biokontrole za poboljšanje zdravlja i produktivnosti biljaka. PB - Novi Sad : Matica srpska T2 - Matica Srpska Journal for Natural Sciences T1 - Screening of Bacillus spp. as potential biocontrol agents against sunflower pathogens T1 - Ispitivanje Bacillus spp. kao potencijalnih agenasa biokontrole patogena suncokreta EP - 72 SP - 65 VL - 143 DO - 10.2298/ZMSPN2243065M ER -
@article{ author = "Miljaković, Dragana and Milošević, Dragana and Ignjatov, Maja and Marinković, Jelena and Tamindžić, Gordana and Tintor, Branislava and Nikolić, Zorica", year = "2022", abstract = "Bacillus spp. are well known to protect plants from seed or soil-borne pathogens by the synthesis of various metabolites with antimicrobial activity, such as hydrolytic enzymes and antibiotics. This study aimed to select the most effective Bacillus spp. from a group of ten antagonistic strains by antifungal activity assay. Bacillus strains were primarily isolated from the soil and identified as B. safensis, B. pumilus and B. subtilis by 16S rDNA sequencing. The four analyzed fungi: Macrophomina phaseolina, Alternaria alternata, Cladosporium cladosporoides, and Sclerotinia sclerotiorum, were obtained from sunflower seeds and identified using PCR analysis and primers specific for ITS region. The antifungal activity of bacterial strains was examined in a dual plate assay. Bacillus spp. demonstrated the highest antagonism against S. sclerotiorum, followed by C. cladosporoides, M. phaseolina, and A. alternata, with an average percentage of growth inhibition (PGI) of 77%, 70%, 64% and 59%, respectively. Overall, Bacillus spp. included in this study demonstrated a rather strong biocontrol potential, although the effect of particular strain varied depending on the tested fungi. The highest antagonistic effect toward M. phaseolina and A. alternata was exhibited by B. safensis B2 and B. pumilus B3. B. pumilus B11 and B. subtilis B32 were the most efficient against C. cladosporoides, whereas B. pumilus B3 and B. subtilis B7 had the highest antifungal activity against S. sclerotiorum. Findings point to the fact that the most effective Bacillus spp. could be used as potential biocontrol agents for improving plant health and productivity., Bacillus spp. suzbijaju fitopatogene sintezom različitih metabolita sa antimikrobnim delovanjem kao što su litički enzimi i antibiotici. Cilj ovih istraživanja bila je selekcija najefektivnijih Bacillus spp. iz grupe antagonističkih sojeva ispitivanjem njihove antifungalne aktivnosti. Bacillus sojevi su prvobitno izolovani iz zemljišta i identifikovani kao B. safensis, B. pumilus i B. subtilis sekvenciranjem 16S rDNA. Analizirane gljive, Macrophomina phaseolina, Alternaria alternata, Cladosporium cladosporoides i Sclerotinia sclerotiorum, izolovane su sa semena suncokreta i identifikovane PCR analizom uz primenu prajmera specifičnih za ITS region. Antifungalna aktivnost bakterija prema fitopatogenim gljivama ispitana je metodom dvojne kultivacije. Bacillus spp. ispoljili su najveći antagonizam prema S. sclerotiorum, zatim C. cladosporoides, M. phaseolina i A. alternata, sa prosečnim procentom inhibicije rasta 77%, 70%, 64% i 59%. Ispitivani sojevi Bacillus spp. pokazali su veoma jak biokontrolni potencijal, iako je efekat određenog soja varirao u zavisnosti od ispitivane gljive. Najveći antagonistički efekat prema M. phaseolina i A. alternata imali su sojevi B. safensis B2 i B. pumilus B3. Najefektivniji sojevi protiv C. cladosporoides bili su B. pumilus B11 i B. subtilis B32, dok je najveća antifungalna aktivnost prema S. sclerotiorum utvrđen primenom sojeva B. pumilus B3 i B. subtilis B7. Rezultati ovih istraživanja pokazuju da se najefektivniji sojevi Bacillus spp. mogu koristiti kao potencijalni agensi biokontrole za poboljšanje zdravlja i produktivnosti biljaka.", publisher = "Novi Sad : Matica srpska", journal = "Matica Srpska Journal for Natural Sciences", title = "Screening of Bacillus spp. as potential biocontrol agents against sunflower pathogens, Ispitivanje Bacillus spp. kao potencijalnih agenasa biokontrole patogena suncokreta", pages = "72-65", volume = "143", doi = "10.2298/ZMSPN2243065M" }
Miljaković, D., Milošević, D., Ignjatov, M., Marinković, J., Tamindžić, G., Tintor, B.,& Nikolić, Z.. (2022). Screening of Bacillus spp. as potential biocontrol agents against sunflower pathogens. in Matica Srpska Journal for Natural Sciences Novi Sad : Matica srpska., 143, 65-72. https://doi.org/10.2298/ZMSPN2243065M
Miljaković D, Milošević D, Ignjatov M, Marinković J, Tamindžić G, Tintor B, Nikolić Z. Screening of Bacillus spp. as potential biocontrol agents against sunflower pathogens. in Matica Srpska Journal for Natural Sciences. 2022;143:65-72. doi:10.2298/ZMSPN2243065M .
Miljaković, Dragana, Milošević, Dragana, Ignjatov, Maja, Marinković, Jelena, Tamindžić, Gordana, Tintor, Branislava, Nikolić, Zorica, "Screening of Bacillus spp. as potential biocontrol agents against sunflower pathogens" in Matica Srpska Journal for Natural Sciences, 143 (2022):65-72, https://doi.org/10.2298/ZMSPN2243065M . .