Towards Sclerotinia resistance: somatic hybridization between wild and cultivated sunflower
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The use of interesting characteristics from wild Helianthus species in sunflower breeding is limited by poor crossability or sterility of interspecific hybrids. Somatic hybridization by protoplast fusion is an efficient method for overcoming crossing barriers and increasing of the genetic variability of the plants. Protoplasts obtained from leaf mesophyll of Sclerotinia sclerotiorum-resistant accessions of Helianthus maximiliani and Helianthus mollis and etiolated hypocotyls of inbred lines PH-BC1-91A and Ha-74A were purified by floating on Ficoll. Prior to electrofusion wild species protoplasts were irradiated with UV light with the aim of inactivating a part of their genome. Fusion products were embedded in agarose droplets and subjected to different regeneration protocols. The best results were obtained with the protocol of Trabace et al. (1995). Developed microcalluses were released from the agarose and transferred to solid media. Shoot regeneration was achieved by culture of callu...ses on regeneration medium containing 2.2 mgL-1 BAP and 0.01 mgL-1 NAA (Krasnyanski et al., 1992). During the culture, calluses were treated with a high concentration of 2,4-D for a limited period. Studies at the molecular and morphological level showed that obtained plants were somatic hybrids.
Кључне речи:
Sclerotinia sclerotiorum / resistance / somatic hybridization / sunflowerИзвор:
Proceedings, 16th International Sunflower Conference, 29 August - 2 September 2004, Fargo, North Dakota, USA, 2004, 2, 737-740Издавач:
- Paris : International Sunflower Association
Колекције
Институција/група
FiVeRTY - CONF AU - Vasić, Dragana AU - Taški-Ajduković, Ksenija AU - Nagl, Nevena AU - Škorić, Dragan PY - 2004 UR - http://fiver.ifvcns.rs/handle/123456789/3201 AB - The use of interesting characteristics from wild Helianthus species in sunflower breeding is limited by poor crossability or sterility of interspecific hybrids. Somatic hybridization by protoplast fusion is an efficient method for overcoming crossing barriers and increasing of the genetic variability of the plants. Protoplasts obtained from leaf mesophyll of Sclerotinia sclerotiorum-resistant accessions of Helianthus maximiliani and Helianthus mollis and etiolated hypocotyls of inbred lines PH-BC1-91A and Ha-74A were purified by floating on Ficoll. Prior to electrofusion wild species protoplasts were irradiated with UV light with the aim of inactivating a part of their genome. Fusion products were embedded in agarose droplets and subjected to different regeneration protocols. The best results were obtained with the protocol of Trabace et al. (1995). Developed microcalluses were released from the agarose and transferred to solid media. Shoot regeneration was achieved by culture of calluses on regeneration medium containing 2.2 mgL-1 BAP and 0.01 mgL-1 NAA (Krasnyanski et al., 1992). During the culture, calluses were treated with a high concentration of 2,4-D for a limited period. Studies at the molecular and morphological level showed that obtained plants were somatic hybrids. PB - Paris : International Sunflower Association C3 - Proceedings, 16th International Sunflower Conference, 29 August - 2 September 2004, Fargo, North Dakota, USA T1 - Towards Sclerotinia resistance: somatic hybridization between wild and cultivated sunflower EP - 740 SP - 737 VL - 2 UR - https://hdl.handle.net/21.15107/rcub_fiver_3201 ER -
@conference{ author = "Vasić, Dragana and Taški-Ajduković, Ksenija and Nagl, Nevena and Škorić, Dragan", year = "2004", abstract = "The use of interesting characteristics from wild Helianthus species in sunflower breeding is limited by poor crossability or sterility of interspecific hybrids. Somatic hybridization by protoplast fusion is an efficient method for overcoming crossing barriers and increasing of the genetic variability of the plants. Protoplasts obtained from leaf mesophyll of Sclerotinia sclerotiorum-resistant accessions of Helianthus maximiliani and Helianthus mollis and etiolated hypocotyls of inbred lines PH-BC1-91A and Ha-74A were purified by floating on Ficoll. Prior to electrofusion wild species protoplasts were irradiated with UV light with the aim of inactivating a part of their genome. Fusion products were embedded in agarose droplets and subjected to different regeneration protocols. The best results were obtained with the protocol of Trabace et al. (1995). Developed microcalluses were released from the agarose and transferred to solid media. Shoot regeneration was achieved by culture of calluses on regeneration medium containing 2.2 mgL-1 BAP and 0.01 mgL-1 NAA (Krasnyanski et al., 1992). During the culture, calluses were treated with a high concentration of 2,4-D for a limited period. Studies at the molecular and morphological level showed that obtained plants were somatic hybrids.", publisher = "Paris : International Sunflower Association", journal = "Proceedings, 16th International Sunflower Conference, 29 August - 2 September 2004, Fargo, North Dakota, USA", title = "Towards Sclerotinia resistance: somatic hybridization between wild and cultivated sunflower", pages = "740-737", volume = "2", url = "https://hdl.handle.net/21.15107/rcub_fiver_3201" }
Vasić, D., Taški-Ajduković, K., Nagl, N.,& Škorić, D.. (2004). Towards Sclerotinia resistance: somatic hybridization between wild and cultivated sunflower. in Proceedings, 16th International Sunflower Conference, 29 August - 2 September 2004, Fargo, North Dakota, USA Paris : International Sunflower Association., 2, 737-740. https://hdl.handle.net/21.15107/rcub_fiver_3201
Vasić D, Taški-Ajduković K, Nagl N, Škorić D. Towards Sclerotinia resistance: somatic hybridization between wild and cultivated sunflower. in Proceedings, 16th International Sunflower Conference, 29 August - 2 September 2004, Fargo, North Dakota, USA. 2004;2:737-740. https://hdl.handle.net/21.15107/rcub_fiver_3201 .
Vasić, Dragana, Taški-Ajduković, Ksenija, Nagl, Nevena, Škorić, Dragan, "Towards Sclerotinia resistance: somatic hybridization between wild and cultivated sunflower" in Proceedings, 16th International Sunflower Conference, 29 August - 2 September 2004, Fargo, North Dakota, USA, 2 (2004):737-740, https://hdl.handle.net/21.15107/rcub_fiver_3201 .