Monitoring the health status of narrow-leaved ash tree seedlings (forest office Morović, locality Vinična) in the early spring of 2015, after the catastrophic floods in May 2014, revealed presence of large dark necrotic areas on 1–2 year old sprouts. The isolation of the fungal pathogen was done by standard phytopathological protocols. Three representative isolates (K41, K42 and K78) were preliminary detected and purified by a single-spore technique for further morphological, molecular analyses and pathogenicity testing. Morphological characteristics classified the isolates as Fusarium tricinctum. Tested isolates on narrow leaved ash sprouts caused reddish brown elongated necrotic lesions averaged 20.1 mm. Two marker genes, translation elongation factor 1-alpha (TEF1-α) and internal transcribed spacer (ITS1), were used in this study. Using the Basic Local Alignment Search Tool (BLAST) searching engine, nucleotide sequences were compared to all related sequences. Alignement score result...ed in 98.9% identities with F. tricinctum for isolate K78, while isolates K41 and K42 showed 94.1% and 94.3% identities with F. tricinctum complex respectively. To the best of our knowledge, this is the first report of F. tricinctum pathogen infection on flood stressed narrow-leaved ash trees in Serbia.
Poljski jasen (Fraxinus angustifolia Vahl.) je drvenasta vrsta rasprostranjena u nizijskim šumama u Evropi. Region jugozapadnog Srema gde se nalaze najvrednije šume ove vrste u Srbiji, bio je izložen katastrofalnim poplavama u maju 2014. Praćenjem zdravstvenog stanja sadnica jasena (šuma Morović, lokalitet „Vinična”) u rano proleće 2015. godine otkriveno je prisustvo velikih tamnih nekrotičnih područja na izbojcima starim 1–2 godine. Sa obolelih izbojaka, standardnim fitopatološkim postupkom, urađena je izolacija patogena. Prema morfološkim karakteristikama dobijeni izolati su determinisani kao Fusarium tricinctum. Za dalje analize su uzeta tri reprezentativna monosporna izolata (K41, K42 i K78). BLAST pretragom NCBI nukleotidne baze utvrđeno je 98,9% sličnosti TEF1-α sekvence izolata K78 (MN822227.1) sa Fusarium tricinctum i 100% poklapanja ITS sekvence ovog izolata (GenBank MK928426.1) sa izolatom F. tricinctum ZMXR6 (MT446111.1). IGS sekvence izo lata K41 i K42 (MZ749901 i MZ749902)... imale su 94,1% i 94,3% sličnosti sa F. tricinctum kompleksom, na osnovu BLAST pretrage FUSARIUM-ID nukleotidne baze. Testovi patogenosti na izbojcima jasena starim godinu dana pokazali su crvenkasto smeđe izdužene nekrotične lezije prosečne dužine 20,1 mm. Patogen je reizolovan iz izbojaka sa simptomima. Prema našim saznanjima, ovo je prvi nalaz F. tricinctum na stablima poljskog jasena u Srbiji.
TY - JOUR
AU - Medić-Pap, Slađana
AU - Živanov, Dalibor
AU - Tančić-Živanov, Sonja
AU - Pap, Predrag
AU - Galović, Vladislava
AU - Nagl, Nevena
AU - Župunski, Vesna
PY - 2022
UR - http://fiver.ifvcns.rs/handle/123456789/3177
AB - Monitoring the health status of narrow-leaved ash tree seedlings (forest office Morović, locality Vinična) in the early spring of 2015, after the catastrophic floods in May 2014, revealed presence of large dark necrotic areas on 1–2 year old sprouts. The isolation of the fungal pathogen was done by standard phytopathological protocols. Three representative isolates (K41, K42 and K78) were preliminary detected and purified by a single-spore technique for further morphological, molecular analyses and pathogenicity testing. Morphological characteristics classified the isolates as Fusarium tricinctum. Tested isolates on narrow leaved ash sprouts caused reddish brown elongated necrotic lesions averaged 20.1 mm. Two marker genes, translation elongation factor 1-alpha (TEF1-α) and internal transcribed spacer (ITS1), were used in this study. Using the Basic Local Alignment Search Tool (BLAST) searching engine, nucleotide sequences were compared to all related sequences. Alignement score resulted in 98.9% identities with F. tricinctum for isolate K78, while isolates K41 and K42 showed 94.1% and 94.3% identities with F. tricinctum complex respectively. To the best of our knowledge, this is the first report of F. tricinctum pathogen infection on flood stressed narrow-leaved ash trees in Serbia.
AB - Poljski jasen (Fraxinus angustifolia Vahl.) je drvenasta vrsta rasprostranjena u nizijskim šumama u Evropi. Region jugozapadnog Srema gde se nalaze najvrednije šume ove vrste u Srbiji, bio je izložen katastrofalnim poplavama u maju 2014. Praćenjem zdravstvenog stanja sadnica jasena (šuma Morović, lokalitet „Vinična”) u rano proleće 2015. godine otkriveno je prisustvo velikih tamnih nekrotičnih područja na izbojcima starim 1–2 godine. Sa obolelih izbojaka, standardnim fitopatološkim postupkom, urađena je izolacija patogena. Prema morfološkim karakteristikama dobijeni izolati su determinisani kao Fusarium tricinctum. Za dalje analize su uzeta tri reprezentativna monosporna izolata (K41, K42 i K78). BLAST pretragom NCBI nukleotidne baze utvrđeno je 98,9% sličnosti TEF1-α sekvence izolata K78 (MN822227.1) sa Fusarium tricinctum i 100% poklapanja ITS sekvence ovog izolata (GenBank MK928426.1) sa izolatom F. tricinctum ZMXR6 (MT446111.1). IGS sekvence izo lata K41 i K42 (MZ749901 i MZ749902) imale su 94,1% i 94,3% sličnosti sa F. tricinctum kompleksom, na osnovu BLAST pretrage FUSARIUM-ID nukleotidne baze. Testovi patogenosti na izbojcima jasena starim godinu dana pokazali su crvenkasto smeđe izdužene nekrotične lezije prosečne dužine 20,1 mm. Patogen je reizolovan iz izbojaka sa simptomima. Prema našim saznanjima, ovo je prvi nalaz F. tricinctum na stablima poljskog jasena u Srbiji.
PB - Novi Sad : Matica srpska
T2 - Zbornik Matice srpske za prirodne nauke
T1 - First report of Fusarium tricinctum on narrow-leaved ash (Fraxinus angustifolia Vahl.) in Serbia
T1 - Prvi nalaz Fusarium tricinctum na poljskom jasenu (Fraxinus angustifolia Vahl.) u Srbiji
EP - 30
SP - 19
VL - 142
DO - 10.2298/ZMSPN2242019M
ER -
@article{
author = "Medić-Pap, Slađana and Živanov, Dalibor and Tančić-Živanov, Sonja and Pap, Predrag and Galović, Vladislava and Nagl, Nevena and Župunski, Vesna",
year = "2022",
abstract = "Monitoring the health status of narrow-leaved ash tree seedlings (forest office Morović, locality Vinična) in the early spring of 2015, after the catastrophic floods in May 2014, revealed presence of large dark necrotic areas on 1–2 year old sprouts. The isolation of the fungal pathogen was done by standard phytopathological protocols. Three representative isolates (K41, K42 and K78) were preliminary detected and purified by a single-spore technique for further morphological, molecular analyses and pathogenicity testing. Morphological characteristics classified the isolates as Fusarium tricinctum. Tested isolates on narrow leaved ash sprouts caused reddish brown elongated necrotic lesions averaged 20.1 mm. Two marker genes, translation elongation factor 1-alpha (TEF1-α) and internal transcribed spacer (ITS1), were used in this study. Using the Basic Local Alignment Search Tool (BLAST) searching engine, nucleotide sequences were compared to all related sequences. Alignement score resulted in 98.9% identities with F. tricinctum for isolate K78, while isolates K41 and K42 showed 94.1% and 94.3% identities with F. tricinctum complex respectively. To the best of our knowledge, this is the first report of F. tricinctum pathogen infection on flood stressed narrow-leaved ash trees in Serbia., Poljski jasen (Fraxinus angustifolia Vahl.) je drvenasta vrsta rasprostranjena u nizijskim šumama u Evropi. Region jugozapadnog Srema gde se nalaze najvrednije šume ove vrste u Srbiji, bio je izložen katastrofalnim poplavama u maju 2014. Praćenjem zdravstvenog stanja sadnica jasena (šuma Morović, lokalitet „Vinična”) u rano proleće 2015. godine otkriveno je prisustvo velikih tamnih nekrotičnih područja na izbojcima starim 1–2 godine. Sa obolelih izbojaka, standardnim fitopatološkim postupkom, urađena je izolacija patogena. Prema morfološkim karakteristikama dobijeni izolati su determinisani kao Fusarium tricinctum. Za dalje analize su uzeta tri reprezentativna monosporna izolata (K41, K42 i K78). BLAST pretragom NCBI nukleotidne baze utvrđeno je 98,9% sličnosti TEF1-α sekvence izolata K78 (MN822227.1) sa Fusarium tricinctum i 100% poklapanja ITS sekvence ovog izolata (GenBank MK928426.1) sa izolatom F. tricinctum ZMXR6 (MT446111.1). IGS sekvence izo lata K41 i K42 (MZ749901 i MZ749902) imale su 94,1% i 94,3% sličnosti sa F. tricinctum kompleksom, na osnovu BLAST pretrage FUSARIUM-ID nukleotidne baze. Testovi patogenosti na izbojcima jasena starim godinu dana pokazali su crvenkasto smeđe izdužene nekrotične lezije prosečne dužine 20,1 mm. Patogen je reizolovan iz izbojaka sa simptomima. Prema našim saznanjima, ovo je prvi nalaz F. tricinctum na stablima poljskog jasena u Srbiji.",
publisher = "Novi Sad : Matica srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "First report of Fusarium tricinctum on narrow-leaved ash (Fraxinus angustifolia Vahl.) in Serbia, Prvi nalaz Fusarium tricinctum na poljskom jasenu (Fraxinus angustifolia Vahl.) u Srbiji",
pages = "30-19",
volume = "142",
doi = "10.2298/ZMSPN2242019M"
}
Medić-Pap, S., Živanov, D., Tančić-Živanov, S., Pap, P., Galović, V., Nagl, N.,& Župunski, V.. (2022). First report of Fusarium tricinctum on narrow-leaved ash (Fraxinus angustifolia Vahl.) in Serbia. in Zbornik Matice srpske za prirodne nauke
Novi Sad : Matica srpska., 142, 19-30.
https://doi.org/10.2298/ZMSPN2242019M
Medić-Pap S, Živanov D, Tančić-Živanov S, Pap P, Galović V, Nagl N, Župunski V. First report of Fusarium tricinctum on narrow-leaved ash (Fraxinus angustifolia Vahl.) in Serbia. in Zbornik Matice srpske za prirodne nauke. 2022;142:19-30.
doi:10.2298/ZMSPN2242019M .
Medić-Pap, Slađana, Živanov, Dalibor, Tančić-Živanov, Sonja, Pap, Predrag, Galović, Vladislava, Nagl, Nevena, Župunski, Vesna, "First report of Fusarium tricinctum on narrow-leaved ash (Fraxinus angustifolia Vahl.) in Serbia" in Zbornik Matice srpske za prirodne nauke, 142 (2022):19-30,
https://doi.org/10.2298/ZMSPN2242019M . .
