Lipidomics-Assisted GWAS (lGWAS) approach for improving high-temperature stress tolerance of crops
Authors
Pranneshraj, VelumaniSangha, Manjeet Kaur
Đalović, Ivica

Miladinović, Jegor

Djanaguiraman, Maduraimuthu
Article (Published version)
Metadata
Show full item recordAbstract
High-temperature stress (HT) over crop productivity is an important environmental factor demanding more attention as recent global warming trends are alarming and pose a potential threat to crop production. According to the Sixth IPCC report, future years will have longer warm seasons and frequent heat waves. Thus, the need arises to develop HT-tolerant genotypes that can be used to breed high-yielding crops. Several physiological, biochemical, and molecular alterations are orchestrated in providing HT tolerance to a genotype. One mechanism to counter HT is overcoming high-temperature-induced membrane superfluidity and structural disorganizations. Several HT lipidomic studies on different genotypes have indicated the potential involvement of membrane lipid remodelling in providing HT tolerance. Advances in high-throughput analytical techniques such as tandem mass spectrometry have paved the way for large-scale identification and quantification of the enormously diverse lipid molecules ...in a single run. Physiological trait-based breeding has been employed so far to identify and select HT tolerant genotypes but has several disadvantages, such as the genotype-phenotype gap affecting the efficiency of identifying the underlying genetic association. Tolerant genotypes maintain a high photosynthetic rate, stable membranes, and membrane-associated mechanisms. In this context, studying the HT-induced membrane lipid remodelling, resultant of several up-/down-regulations of genes and post-translational modifications, will aid in identifying potential lipid biomarkers for HT tolerance/susceptibility. The identified lipid biomarkers (LIPIDOTYPE) can thus be considered an intermediate phenotype, bridging the gap between genotype–phenotype (genotype–LIPIDOTYPE–phenotype). Recent works integrating metabolomics with quantitative genetic studies such as GWAS (mGWAS) have provided close associations between genotype, metabolites, and stress-tolerant phenotypes. This review has been sculpted to provide a potential workflow that combines MS-based lipidomics and the robust GWAS (lipidomics assisted GWAS-lGWAS) to identify membrane lipid remodelling related genes and associations which can be used to develop HS tolerant genotypes with enhanced membrane thermostability (MTS) and heat stable photosynthesis (HP).
Keywords:
high temperature / tolerance mechanism / membrane lipids / lipidomics / photosynthesis / genotypes / phenotypes / GWAS / breedingSource:
International Journal of Molecular Sciences, 2022, 23, 9389-Publisher:
- Basel : MDPI
Funding / projects:
- BT/PR36115/NNT/28/ 1814/2021 the Department of Biotechnology, Ministry of Science and Technology, Government of India
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200032 (Institute of Field and Vegetable Crops, Novi Sad) (RS-200032)
DOI: 10.3390/ijms23169389
ISSN: 1422-0067; 1661-6596
WoS: 00084658030000
Scopus: 2-s2.0-85137673988
Collections
Institution/Community
FiVeRTY - JOUR AU - Pranneshraj, Velumani AU - Sangha, Manjeet Kaur AU - Đalović, Ivica AU - Miladinović, Jegor AU - Djanaguiraman, Maduraimuthu PY - 2022 UR - http://fiver.ifvcns.rs/handle/123456789/3080 AB - High-temperature stress (HT) over crop productivity is an important environmental factor demanding more attention as recent global warming trends are alarming and pose a potential threat to crop production. According to the Sixth IPCC report, future years will have longer warm seasons and frequent heat waves. Thus, the need arises to develop HT-tolerant genotypes that can be used to breed high-yielding crops. Several physiological, biochemical, and molecular alterations are orchestrated in providing HT tolerance to a genotype. One mechanism to counter HT is overcoming high-temperature-induced membrane superfluidity and structural disorganizations. Several HT lipidomic studies on different genotypes have indicated the potential involvement of membrane lipid remodelling in providing HT tolerance. Advances in high-throughput analytical techniques such as tandem mass spectrometry have paved the way for large-scale identification and quantification of the enormously diverse lipid molecules in a single run. Physiological trait-based breeding has been employed so far to identify and select HT tolerant genotypes but has several disadvantages, such as the genotype-phenotype gap affecting the efficiency of identifying the underlying genetic association. Tolerant genotypes maintain a high photosynthetic rate, stable membranes, and membrane-associated mechanisms. In this context, studying the HT-induced membrane lipid remodelling, resultant of several up-/down-regulations of genes and post-translational modifications, will aid in identifying potential lipid biomarkers for HT tolerance/susceptibility. The identified lipid biomarkers (LIPIDOTYPE) can thus be considered an intermediate phenotype, bridging the gap between genotype–phenotype (genotype–LIPIDOTYPE–phenotype). Recent works integrating metabolomics with quantitative genetic studies such as GWAS (mGWAS) have provided close associations between genotype, metabolites, and stress-tolerant phenotypes. This review has been sculpted to provide a potential workflow that combines MS-based lipidomics and the robust GWAS (lipidomics assisted GWAS-lGWAS) to identify membrane lipid remodelling related genes and associations which can be used to develop HS tolerant genotypes with enhanced membrane thermostability (MTS) and heat stable photosynthesis (HP). PB - Basel : MDPI T2 - International Journal of Molecular Sciences T1 - Lipidomics-Assisted GWAS (lGWAS) approach for improving high-temperature stress tolerance of crops SP - 9389 VL - 23 DO - 10.3390/ijms23169389 ER -
@article{ author = "Pranneshraj, Velumani and Sangha, Manjeet Kaur and Đalović, Ivica and Miladinović, Jegor and Djanaguiraman, Maduraimuthu", year = "2022", abstract = "High-temperature stress (HT) over crop productivity is an important environmental factor demanding more attention as recent global warming trends are alarming and pose a potential threat to crop production. According to the Sixth IPCC report, future years will have longer warm seasons and frequent heat waves. Thus, the need arises to develop HT-tolerant genotypes that can be used to breed high-yielding crops. Several physiological, biochemical, and molecular alterations are orchestrated in providing HT tolerance to a genotype. One mechanism to counter HT is overcoming high-temperature-induced membrane superfluidity and structural disorganizations. Several HT lipidomic studies on different genotypes have indicated the potential involvement of membrane lipid remodelling in providing HT tolerance. Advances in high-throughput analytical techniques such as tandem mass spectrometry have paved the way for large-scale identification and quantification of the enormously diverse lipid molecules in a single run. Physiological trait-based breeding has been employed so far to identify and select HT tolerant genotypes but has several disadvantages, such as the genotype-phenotype gap affecting the efficiency of identifying the underlying genetic association. Tolerant genotypes maintain a high photosynthetic rate, stable membranes, and membrane-associated mechanisms. In this context, studying the HT-induced membrane lipid remodelling, resultant of several up-/down-regulations of genes and post-translational modifications, will aid in identifying potential lipid biomarkers for HT tolerance/susceptibility. The identified lipid biomarkers (LIPIDOTYPE) can thus be considered an intermediate phenotype, bridging the gap between genotype–phenotype (genotype–LIPIDOTYPE–phenotype). Recent works integrating metabolomics with quantitative genetic studies such as GWAS (mGWAS) have provided close associations between genotype, metabolites, and stress-tolerant phenotypes. This review has been sculpted to provide a potential workflow that combines MS-based lipidomics and the robust GWAS (lipidomics assisted GWAS-lGWAS) to identify membrane lipid remodelling related genes and associations which can be used to develop HS tolerant genotypes with enhanced membrane thermostability (MTS) and heat stable photosynthesis (HP).", publisher = "Basel : MDPI", journal = "International Journal of Molecular Sciences", title = "Lipidomics-Assisted GWAS (lGWAS) approach for improving high-temperature stress tolerance of crops", pages = "9389", volume = "23", doi = "10.3390/ijms23169389" }
Pranneshraj, V., Sangha, M. K., Đalović, I., Miladinović, J.,& Djanaguiraman, M.. (2022). Lipidomics-Assisted GWAS (lGWAS) approach for improving high-temperature stress tolerance of crops. in International Journal of Molecular Sciences Basel : MDPI., 23, 9389. https://doi.org/10.3390/ijms23169389
Pranneshraj V, Sangha MK, Đalović I, Miladinović J, Djanaguiraman M. Lipidomics-Assisted GWAS (lGWAS) approach for improving high-temperature stress tolerance of crops. in International Journal of Molecular Sciences. 2022;23:9389. doi:10.3390/ijms23169389 .
Pranneshraj, Velumani, Sangha, Manjeet Kaur, Đalović, Ivica, Miladinović, Jegor, Djanaguiraman, Maduraimuthu, "Lipidomics-Assisted GWAS (lGWAS) approach for improving high-temperature stress tolerance of crops" in International Journal of Molecular Sciences, 23 (2022):9389, https://doi.org/10.3390/ijms23169389 . .