Introducing a cut-stem inoculation method for fast evaluation of sunflower resistance to Macrophomina phaseolina
Аутори
Ćuk, NemanjaCvejić, Sandra
Mladenov, Velimir
Miladinović, Dragana
Babec, Brankica
Jocić, Siniša
Dedić, Boško
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт
The fast adaptation to different growing conditions of a fungus Macrophomina phaseolina, led to its becoming one of the sunflower (Helianthus annuus L.) disease causal agents in regions with a temperate climate. Methods currently used to determine sunflower resistance require laborious manual inoculation and confirmation of pathogen appearance, due to the late stage of testing. The paper proposes a cut-stem method for inoculating sunflower plants in the controlled conditions and the possibility of early-stage disease evaluation. A set of 15 sunflower inbred lines was inoculated using M. phaselolina isolate in the growth chamber and the obtained data were analysed using Cut-stem Disease Severity (CSDS) and compared with disease severity obtained from field experiments using traditional inoculation methods (toothpick, Unwounded Stem Base Inoculation (USBI) and non-inoculated plants). The results showed that, based on CSDS, inbred lines infected with the cut-stem inoculation method signif...icantly differed regarding resistance to M. phaseolina. None of the inbred lines exhibited complete resistance but three lines could be proposed as a source of resistance to this pathogen. Ranking of inbred lines which was based on resistance to M.phaseolina was similar in all inoculation methods and in non-inoculated plants. There were highly significant correlations between the values obtained from growth chamber experiment and disease severity scores from field evaluations. Thus, the obtained results indicate that the cut-stem method could potentially complement field testing methods and be valuable tool in sunflower breeding for resistance to M. phaseolina.
Кључне речи:
charcoal rot / diseases / disease progress / disease severity / progress / severity / early-stage evaluation / early evaluation / inbred lines / inoculation / sunflower / Macrophomina phaseolina / cut-stem inoculationИзвор:
Phytoparasitica, 2022Издавач:
- Springer
Финансирање / пројекти:
- SmartSun - Creating climate smart sunflower for future challenges (RS-ScienceFundRS-Ideje-7732457)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200032 (Научни институт за ратарство и повртарство, Нови Сад) (RS-MESTD-inst-2020-200032)
- Climate Crops - Centre of Excellence for Innovations in Breeding of Climate-Resilient Crops, Institute of Field and Vegetable Crops
Напомена:
- This is the accepted peer-reviewed version of the article available after 28 July 2023. The published version is http://fiver.ifvcns.rs/handle/123456789/2970.
DOI: 10.1007/s12600-022-01015-0
ISSN: 0334-2123; 1876-7184
WoS: 00083097120000
Scopus: 2-s2.0-85135253438
Колекције
Институција/група
FiVeRTY - JOUR AU - Ćuk, Nemanja AU - Cvejić, Sandra AU - Mladenov, Velimir AU - Miladinović, Dragana AU - Babec, Brankica AU - Jocić, Siniša AU - Dedić, Boško PY - 2022 UR - http://fiver.ifvcns.rs/handle/123456789/2971 AB - The fast adaptation to different growing conditions of a fungus Macrophomina phaseolina, led to its becoming one of the sunflower (Helianthus annuus L.) disease causal agents in regions with a temperate climate. Methods currently used to determine sunflower resistance require laborious manual inoculation and confirmation of pathogen appearance, due to the late stage of testing. The paper proposes a cut-stem method for inoculating sunflower plants in the controlled conditions and the possibility of early-stage disease evaluation. A set of 15 sunflower inbred lines was inoculated using M. phaselolina isolate in the growth chamber and the obtained data were analysed using Cut-stem Disease Severity (CSDS) and compared with disease severity obtained from field experiments using traditional inoculation methods (toothpick, Unwounded Stem Base Inoculation (USBI) and non-inoculated plants). The results showed that, based on CSDS, inbred lines infected with the cut-stem inoculation method significantly differed regarding resistance to M. phaseolina. None of the inbred lines exhibited complete resistance but three lines could be proposed as a source of resistance to this pathogen. Ranking of inbred lines which was based on resistance to M.phaseolina was similar in all inoculation methods and in non-inoculated plants. There were highly significant correlations between the values obtained from growth chamber experiment and disease severity scores from field evaluations. Thus, the obtained results indicate that the cut-stem method could potentially complement field testing methods and be valuable tool in sunflower breeding for resistance to M. phaseolina. PB - Springer T2 - Phytoparasitica T1 - Introducing a cut-stem inoculation method for fast evaluation of sunflower resistance to Macrophomina phaseolina DO - 10.1007/s12600-022-01015-0 ER -
@article{ author = "Ćuk, Nemanja and Cvejić, Sandra and Mladenov, Velimir and Miladinović, Dragana and Babec, Brankica and Jocić, Siniša and Dedić, Boško", year = "2022", abstract = "The fast adaptation to different growing conditions of a fungus Macrophomina phaseolina, led to its becoming one of the sunflower (Helianthus annuus L.) disease causal agents in regions with a temperate climate. Methods currently used to determine sunflower resistance require laborious manual inoculation and confirmation of pathogen appearance, due to the late stage of testing. The paper proposes a cut-stem method for inoculating sunflower plants in the controlled conditions and the possibility of early-stage disease evaluation. A set of 15 sunflower inbred lines was inoculated using M. phaselolina isolate in the growth chamber and the obtained data were analysed using Cut-stem Disease Severity (CSDS) and compared with disease severity obtained from field experiments using traditional inoculation methods (toothpick, Unwounded Stem Base Inoculation (USBI) and non-inoculated plants). The results showed that, based on CSDS, inbred lines infected with the cut-stem inoculation method significantly differed regarding resistance to M. phaseolina. None of the inbred lines exhibited complete resistance but three lines could be proposed as a source of resistance to this pathogen. Ranking of inbred lines which was based on resistance to M.phaseolina was similar in all inoculation methods and in non-inoculated plants. There were highly significant correlations between the values obtained from growth chamber experiment and disease severity scores from field evaluations. Thus, the obtained results indicate that the cut-stem method could potentially complement field testing methods and be valuable tool in sunflower breeding for resistance to M. phaseolina.", publisher = "Springer", journal = "Phytoparasitica", title = "Introducing a cut-stem inoculation method for fast evaluation of sunflower resistance to Macrophomina phaseolina", doi = "10.1007/s12600-022-01015-0" }
Ćuk, N., Cvejić, S., Mladenov, V., Miladinović, D., Babec, B., Jocić, S.,& Dedić, B.. (2022). Introducing a cut-stem inoculation method for fast evaluation of sunflower resistance to Macrophomina phaseolina. in Phytoparasitica Springer.. https://doi.org/10.1007/s12600-022-01015-0
Ćuk N, Cvejić S, Mladenov V, Miladinović D, Babec B, Jocić S, Dedić B. Introducing a cut-stem inoculation method for fast evaluation of sunflower resistance to Macrophomina phaseolina. in Phytoparasitica. 2022;. doi:10.1007/s12600-022-01015-0 .
Ćuk, Nemanja, Cvejić, Sandra, Mladenov, Velimir, Miladinović, Dragana, Babec, Brankica, Jocić, Siniša, Dedić, Boško, "Introducing a cut-stem inoculation method for fast evaluation of sunflower resistance to Macrophomina phaseolina" in Phytoparasitica (2022), https://doi.org/10.1007/s12600-022-01015-0 . .