Colonization of sunflower seed with Alternaria alternata
Аутори
Milošević, DraganaIgnjatov, Maja
Miklič, Vladimir
Karaman, Maja
Nikolić, Zorica
Tamindžić, Gordana
Dedić, Boško
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Sunflower (Helianthus annuus L.) is one of the most important oilseed crops throughout the world, and in Serbia it is grown on about 160,000 to 210,000 ha. Alternaria alternata (Fr.) Keissler has been detected as the most frequent seed-borne pathogen, able to infect a wide variety of plant species in different countries. During a routine seed quality control and seed health testing analysis of sunflower seed in 2018-2019, according to ISTA Rules, Alternaria spp. infection was observed on an average of 18%. The aim of this study was the isolation and identification of Alternaria spp. based on their morphological characteristics and molecular analyses. Isolation of the pathogen was carried out by transferring infected seeds onto a potato dextrose agar (PDA), and subsequent incubation at 25°C. Seven days later, 21 isolates formed circular, smooth, grey to brownish-black colonies, with concentric zones of very intensive sporulation. Conidiophores were branched, solitary or in small groups,... brown to olivaceous brown, ranging from 128 to 232 µm. All isolates were subcultured onto a PDA using a single spore technique.
Кључне речи:
Alternaria / A. alternata / sunflower / seeds / molecular identification / ITS / sequencingИзвор:
Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia, 2022, 206-206Издавач:
- Paris : International Sunflower Association
- Novi Sad : Institute of Field and Vegetable Crops
Финансирање / пројекти:
- Развој нових сорти и побољшање технологија производње уљаних биљних врста за различите намене (RS-MESTD-Technological Development (TD or TR)-31025)
Колекције
Институција/група
FiVeRTY - CONF AU - Milošević, Dragana AU - Ignjatov, Maja AU - Miklič, Vladimir AU - Karaman, Maja AU - Nikolić, Zorica AU - Tamindžić, Gordana AU - Dedić, Boško PY - 2022 UR - http://fiver.ifvcns.rs/handle/123456789/2910 AB - Sunflower (Helianthus annuus L.) is one of the most important oilseed crops throughout the world, and in Serbia it is grown on about 160,000 to 210,000 ha. Alternaria alternata (Fr.) Keissler has been detected as the most frequent seed-borne pathogen, able to infect a wide variety of plant species in different countries. During a routine seed quality control and seed health testing analysis of sunflower seed in 2018-2019, according to ISTA Rules, Alternaria spp. infection was observed on an average of 18%. The aim of this study was the isolation and identification of Alternaria spp. based on their morphological characteristics and molecular analyses. Isolation of the pathogen was carried out by transferring infected seeds onto a potato dextrose agar (PDA), and subsequent incubation at 25°C. Seven days later, 21 isolates formed circular, smooth, grey to brownish-black colonies, with concentric zones of very intensive sporulation. Conidiophores were branched, solitary or in small groups, brown to olivaceous brown, ranging from 128 to 232 µm. All isolates were subcultured onto a PDA using a single spore technique. PB - Paris : International Sunflower Association PB - Novi Sad : Institute of Field and Vegetable Crops C3 - Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia T1 - Colonization of sunflower seed with Alternaria alternata EP - 206 SP - 206 UR - https://hdl.handle.net/21.15107/rcub_fiver_2910 ER -
@conference{ author = "Milošević, Dragana and Ignjatov, Maja and Miklič, Vladimir and Karaman, Maja and Nikolić, Zorica and Tamindžić, Gordana and Dedić, Boško", year = "2022", abstract = "Sunflower (Helianthus annuus L.) is one of the most important oilseed crops throughout the world, and in Serbia it is grown on about 160,000 to 210,000 ha. Alternaria alternata (Fr.) Keissler has been detected as the most frequent seed-borne pathogen, able to infect a wide variety of plant species in different countries. During a routine seed quality control and seed health testing analysis of sunflower seed in 2018-2019, according to ISTA Rules, Alternaria spp. infection was observed on an average of 18%. The aim of this study was the isolation and identification of Alternaria spp. based on their morphological characteristics and molecular analyses. Isolation of the pathogen was carried out by transferring infected seeds onto a potato dextrose agar (PDA), and subsequent incubation at 25°C. Seven days later, 21 isolates formed circular, smooth, grey to brownish-black colonies, with concentric zones of very intensive sporulation. Conidiophores were branched, solitary or in small groups, brown to olivaceous brown, ranging from 128 to 232 µm. All isolates were subcultured onto a PDA using a single spore technique.", publisher = "Paris : International Sunflower Association, Novi Sad : Institute of Field and Vegetable Crops", journal = "Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia", title = "Colonization of sunflower seed with Alternaria alternata", pages = "206-206", url = "https://hdl.handle.net/21.15107/rcub_fiver_2910" }
Milošević, D., Ignjatov, M., Miklič, V., Karaman, M., Nikolić, Z., Tamindžić, G.,& Dedić, B.. (2022). Colonization of sunflower seed with Alternaria alternata. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia Paris : International Sunflower Association., 206-206. https://hdl.handle.net/21.15107/rcub_fiver_2910
Milošević D, Ignjatov M, Miklič V, Karaman M, Nikolić Z, Tamindžić G, Dedić B. Colonization of sunflower seed with Alternaria alternata. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia. 2022;:206-206. https://hdl.handle.net/21.15107/rcub_fiver_2910 .
Milošević, Dragana, Ignjatov, Maja, Miklič, Vladimir, Karaman, Maja, Nikolić, Zorica, Tamindžić, Gordana, Dedić, Boško, "Colonization of sunflower seed with Alternaria alternata" in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia (2022):206-206, https://hdl.handle.net/21.15107/rcub_fiver_2910 .