Preservation and molecular characterization of 13 central Balkan landraces of faba bean (Vicia faba L.)
2013
Аутори
Aleksić, JelenaBanović, Bojana
Mikić, Aleksandar
Ćupina, Branko
Zlatković, Bojan
Anđelković, Snežana
Maksimović, Vesna
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
An increasing interest in cultivation and improvement of faba bean (Vicia faba L.), an important food and feed grain legume whose ex siz genetic resources comprise cultivated forms only, triggers both search for novel genotypes and their molecular characterization. Due to the poor representation of faba bean genotypes from the central Balkans in worldwide ex situ collections, we collected seeds of 13 faba bean landraces from this region, preserved them at the French National Institute for Agricultural Research (INRA) ex situ collection and used them also for amplification of ten nuclear microsatellites (simple sequence repeats — SSRs). Given the self-pollinating nature of faba bean and low heterozygosity levels in this crop, we assumed that relatively low number of alleles may be expected in each landrace. Therefore, we first demonstrated that all alleles obtained upon amplification of ten SSRs in 11 individuals from one landrace could be detected when DNAs from these individuals were ...pooled and such a bulk DNA was used as a template for PCR reactions, and then tested all SSRs in bulk DNA templates from each out of ten remaining landraces comprising six to 15 genotypes.
Кључне речи:
preservation / molecular characterization / Balkans / landraces / faba bean / Vicia faba / grain legumesИзвор:
Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad, 2013, 19-19Издавач:
- International Legume Society
- Novi Sad : Institute of Field and Vegetable Crops
Финансирање / пројекти:
- Молекуларни механизми одговора биљака на абиотички стрес-улога транскрипционих фактора и малих РНК и анализа генетичког диверзитета биљних култура од интереса за пољопривреду и биотехнологију (RS-MESTD-Basic Research (BR or ON)-173005)
- Bilateral project Serbia - France, Pavle Savić Programme: FABAGRALE, Characterisation of faba bean genetic resources (Vicia faba L.) to support genetic improvement of this agronomic legume, 680-00-132/2012-09/15 (2012-2013)
Колекције
Институција/група
FiVeRTY - CONF AU - Aleksić, Jelena AU - Banović, Bojana AU - Mikić, Aleksandar AU - Ćupina, Branko AU - Zlatković, Bojan AU - Anđelković, Snežana AU - Maksimović, Vesna PY - 2013 UR - http://fiver.ifvcns.rs/handle/123456789/2862 AB - An increasing interest in cultivation and improvement of faba bean (Vicia faba L.), an important food and feed grain legume whose ex siz genetic resources comprise cultivated forms only, triggers both search for novel genotypes and their molecular characterization. Due to the poor representation of faba bean genotypes from the central Balkans in worldwide ex situ collections, we collected seeds of 13 faba bean landraces from this region, preserved them at the French National Institute for Agricultural Research (INRA) ex situ collection and used them also for amplification of ten nuclear microsatellites (simple sequence repeats — SSRs). Given the self-pollinating nature of faba bean and low heterozygosity levels in this crop, we assumed that relatively low number of alleles may be expected in each landrace. Therefore, we first demonstrated that all alleles obtained upon amplification of ten SSRs in 11 individuals from one landrace could be detected when DNAs from these individuals were pooled and such a bulk DNA was used as a template for PCR reactions, and then tested all SSRs in bulk DNA templates from each out of ten remaining landraces comprising six to 15 genotypes. PB - International Legume Society PB - Novi Sad : Institute of Field and Vegetable Crops C3 - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad T1 - Preservation and molecular characterization of 13 central Balkan landraces of faba bean (Vicia faba L.) EP - 19 SP - 19 UR - https://hdl.handle.net/21.15107/rcub_fiver_2862 ER -
@conference{ author = "Aleksić, Jelena and Banović, Bojana and Mikić, Aleksandar and Ćupina, Branko and Zlatković, Bojan and Anđelković, Snežana and Maksimović, Vesna", year = "2013", abstract = "An increasing interest in cultivation and improvement of faba bean (Vicia faba L.), an important food and feed grain legume whose ex siz genetic resources comprise cultivated forms only, triggers both search for novel genotypes and their molecular characterization. Due to the poor representation of faba bean genotypes from the central Balkans in worldwide ex situ collections, we collected seeds of 13 faba bean landraces from this region, preserved them at the French National Institute for Agricultural Research (INRA) ex situ collection and used them also for amplification of ten nuclear microsatellites (simple sequence repeats — SSRs). Given the self-pollinating nature of faba bean and low heterozygosity levels in this crop, we assumed that relatively low number of alleles may be expected in each landrace. Therefore, we first demonstrated that all alleles obtained upon amplification of ten SSRs in 11 individuals from one landrace could be detected when DNAs from these individuals were pooled and such a bulk DNA was used as a template for PCR reactions, and then tested all SSRs in bulk DNA templates from each out of ten remaining landraces comprising six to 15 genotypes.", publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops", journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad", title = "Preservation and molecular characterization of 13 central Balkan landraces of faba bean (Vicia faba L.)", pages = "19-19", url = "https://hdl.handle.net/21.15107/rcub_fiver_2862" }
Aleksić, J., Banović, B., Mikić, A., Ćupina, B., Zlatković, B., Anđelković, S.,& Maksimović, V.. (2013). Preservation and molecular characterization of 13 central Balkan landraces of faba bean (Vicia faba L.). in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad International Legume Society., 19-19. https://hdl.handle.net/21.15107/rcub_fiver_2862
Aleksić J, Banović B, Mikić A, Ćupina B, Zlatković B, Anđelković S, Maksimović V. Preservation and molecular characterization of 13 central Balkan landraces of faba bean (Vicia faba L.). in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:19-19. https://hdl.handle.net/21.15107/rcub_fiver_2862 .
Aleksić, Jelena, Banović, Bojana, Mikić, Aleksandar, Ćupina, Branko, Zlatković, Bojan, Anđelković, Snežana, Maksimović, Vesna, "Preservation and molecular characterization of 13 central Balkan landraces of faba bean (Vicia faba L.)" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):19-19, https://hdl.handle.net/21.15107/rcub_fiver_2862 .