Morphological and molecular characterization of Fusarium graminearum Schwabe as a causal agent of Hyssopus officinalis L. seed rot

Abstract

Symptoms of seed rot of Hyssopus officinalis L. were noticed during seed health testing in 2018. According to morphological and cultural characteristics, isolates belong to Fusarium spp. and Alternaria spp.. Based on morphological and pathogenic properties, as well as sequence analysis, isolate designated as 4003/3 wаs identified as Fusarium graminearum deposited in NCBI gene bank under Acc. Number MK061542. To our knowledge F. graminearum as the causal agent of Hyssopus officinalis L. seed rot in Serbia was noticed for the first time.

Simptomi truleži semena Hyssopus officinalis L. primećeni su tokom ispitivanja zdravstvenog stanja semena 2018. godine. Prema morfološkim i odgajivačkim karakteristikama izolati pripadaju vrstama Fusarium spp. i Alternatia spp.. Identifikacija Fusarium spp. potvrđena je primenom lančane reakcije polimeraze sa parom prajmera EF1 i EF2, pri čemu je amplifikacija i sekvencioniranje gena TEF-1α izvršena za izolat JBL4003/3 (MK061542.1), čime je potvrđeno da je izop novi domaćin vrste Fusarium graminearum Schwabe.