Efficacy of Trichoderma spp. isolates against Sclerotinia sclerotiorum on sunflower seedlings
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Trichoderma species are well known as effective antagonists to a variety of soil fungal pathogens. The aim of this research was to test the ability of Trichoderma spp. isolates, which previously indicated antagonistic activity (Tančić et al. 2012), to protect sunflower seedlings from Sclerotinia sclerotiorum. Ten Trichoderma spp. isolates obtained from different soil types and localities in Vojvodina province and one S. sclerotiorum isolate from sunflower grown at Rimski Šančevi (Serbia) were used in research. Biological efficacy was tested on 100 sunflower seeds treated with Trichoderma spp. suspensions (1x106) in two different treatments: T-30 (modified Mukhtar et al., 2012) and T-1.2 (Maslienko, 2005). Trichoderma-coated seeds were placed in four replicates on wet filter paper in Petri dishes. Next to each Trichoderma-coated seed the 5 mm2 plug of S. sclerotiorum mycelia was placed, and incubated under the optimal laboratory conditions. Seeds treated with sterile distilled water wit...h pathogen and without it were used as a positive and negative control, respectively. After seven days, biological efficacy of Trichoderma spp. isolates was assessed and calculated according to Liu et al. (2009). According to obtained results, biological efficacy of all tested Trichoderma isolates was statistically significant as compared to the positive control in both treatments. Good antagonism with over 50% of biological efficacy was registered in 8 isolates in T-30, and 3 isolates in treatment T-1.2. Three Trichoderma isolates which showed biological efficacy over 50% in both treatments can be considered as potential biocontrol agents which should be included in further more comprehensive research.
Keywords:
Trichoderma / Sclerotinia sclerotiorum / antagonism / sunflower / isolates / seedlingsSource:
Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey, 2016, 942-947Publisher:
- Paris : International Sunflower Association
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FiVeRTY - CONF AU - Tančić-Živanov, Sonja AU - Jocić, Siniša AU - Miklič, Vladimir PY - 2016 UR - http://fiver.ifvcns.rs/handle/123456789/2775 AB - Trichoderma species are well known as effective antagonists to a variety of soil fungal pathogens. The aim of this research was to test the ability of Trichoderma spp. isolates, which previously indicated antagonistic activity (Tančić et al. 2012), to protect sunflower seedlings from Sclerotinia sclerotiorum. Ten Trichoderma spp. isolates obtained from different soil types and localities in Vojvodina province and one S. sclerotiorum isolate from sunflower grown at Rimski Šančevi (Serbia) were used in research. Biological efficacy was tested on 100 sunflower seeds treated with Trichoderma spp. suspensions (1x106) in two different treatments: T-30 (modified Mukhtar et al., 2012) and T-1.2 (Maslienko, 2005). Trichoderma-coated seeds were placed in four replicates on wet filter paper in Petri dishes. Next to each Trichoderma-coated seed the 5 mm2 plug of S. sclerotiorum mycelia was placed, and incubated under the optimal laboratory conditions. Seeds treated with sterile distilled water with pathogen and without it were used as a positive and negative control, respectively. After seven days, biological efficacy of Trichoderma spp. isolates was assessed and calculated according to Liu et al. (2009). According to obtained results, biological efficacy of all tested Trichoderma isolates was statistically significant as compared to the positive control in both treatments. Good antagonism with over 50% of biological efficacy was registered in 8 isolates in T-30, and 3 isolates in treatment T-1.2. Three Trichoderma isolates which showed biological efficacy over 50% in both treatments can be considered as potential biocontrol agents which should be included in further more comprehensive research. PB - Paris : International Sunflower Association C3 - Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey T1 - Efficacy of Trichoderma spp. isolates against Sclerotinia sclerotiorum on sunflower seedlings EP - 947 SP - 942 UR - https://hdl.handle.net/21.15107/rcub_fiver_2775 ER -
@conference{ author = "Tančić-Živanov, Sonja and Jocić, Siniša and Miklič, Vladimir", year = "2016", abstract = "Trichoderma species are well known as effective antagonists to a variety of soil fungal pathogens. The aim of this research was to test the ability of Trichoderma spp. isolates, which previously indicated antagonistic activity (Tančić et al. 2012), to protect sunflower seedlings from Sclerotinia sclerotiorum. Ten Trichoderma spp. isolates obtained from different soil types and localities in Vojvodina province and one S. sclerotiorum isolate from sunflower grown at Rimski Šančevi (Serbia) were used in research. Biological efficacy was tested on 100 sunflower seeds treated with Trichoderma spp. suspensions (1x106) in two different treatments: T-30 (modified Mukhtar et al., 2012) and T-1.2 (Maslienko, 2005). Trichoderma-coated seeds were placed in four replicates on wet filter paper in Petri dishes. Next to each Trichoderma-coated seed the 5 mm2 plug of S. sclerotiorum mycelia was placed, and incubated under the optimal laboratory conditions. Seeds treated with sterile distilled water with pathogen and without it were used as a positive and negative control, respectively. After seven days, biological efficacy of Trichoderma spp. isolates was assessed and calculated according to Liu et al. (2009). According to obtained results, biological efficacy of all tested Trichoderma isolates was statistically significant as compared to the positive control in both treatments. Good antagonism with over 50% of biological efficacy was registered in 8 isolates in T-30, and 3 isolates in treatment T-1.2. Three Trichoderma isolates which showed biological efficacy over 50% in both treatments can be considered as potential biocontrol agents which should be included in further more comprehensive research.", publisher = "Paris : International Sunflower Association", journal = "Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey", title = "Efficacy of Trichoderma spp. isolates against Sclerotinia sclerotiorum on sunflower seedlings", pages = "947-942", url = "https://hdl.handle.net/21.15107/rcub_fiver_2775" }
Tančić-Živanov, S., Jocić, S.,& Miklič, V.. (2016). Efficacy of Trichoderma spp. isolates against Sclerotinia sclerotiorum on sunflower seedlings. in Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey Paris : International Sunflower Association., 942-947. https://hdl.handle.net/21.15107/rcub_fiver_2775
Tančić-Živanov S, Jocić S, Miklič V. Efficacy of Trichoderma spp. isolates against Sclerotinia sclerotiorum on sunflower seedlings. in Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey. 2016;:942-947. https://hdl.handle.net/21.15107/rcub_fiver_2775 .
Tančić-Živanov, Sonja, Jocić, Siniša, Miklič, Vladimir, "Efficacy of Trichoderma spp. isolates against Sclerotinia sclerotiorum on sunflower seedlings" in Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey (2016):942-947, https://hdl.handle.net/21.15107/rcub_fiver_2775 .