The use of PCR-based markers in the evaluation of resistance to downy mildew in ns-breeding material
Апстракт
The aim of this work was the application of previously developed and development of new PCR markers for the evaluation of sunflower resistance to downy mildew. Twenty sunflower inbred lines were investigated. Plant resistance to downy mildew was determined by the whole seedling immersion method. Genomic DNA was extracted from the first pair of leaves, and its polymorphism was investigated by RAPD, SSR and several published markers for disease resistance. The presence of the markers Ha-NBS 7/R, Ha-NBS 8/R Ha-NBS 9/R, in Pl6 donor lines (Ha-335, JM-8) and in resistant progeny (Ha-26, G12, G10, G11) confirm that HAP3 could be useful for the detection of the Pl6 gene. DNA polymorphism, which coincided with disease resistance, was revealed with one RAPD (UBC 119 fragment 900-1000 bp) and one SSR primer (ORS37 fragment 600-700 bp). Amplified fragments segregated in the same way i.e., they appeared in 50% of the resistant genotypes. The non-expecting SSR fragment was purified, cloned and sequ...enced. The results indicated that this fragment is not a part of a coding sequence. Specific primers for the amplification of this fragment have been designed and the investigation of the inheritance of this SCAR marker is under way. None of the applied markers appeared in all resistant genotypes. In order to select lines for making crosses for use in further investigation, the obtained results were also used for the calculation of genetic distances between genotypes (simple matching coefficient) and the construction of a dendrogram (UPGMA method).
Кључне речи:
sunflower / downy mildew / RAPD / SSR / DNA polymorphism / cluster analysisИзвор:
Helia, 2004, 27, 40, 149-158Издавач:
- Institut za ratarstvo i povrtarstvo, Novi Sad
Колекције
Институција/група
FiVeRTY - JOUR AU - Panković, Dejana AU - Jocić, Siniša AU - Lačok, Nada AU - Sakač, Zvonimir AU - Škorić, Dragan PY - 2004 UR - http://fiver.ifvcns.rs/handle/123456789/272 AB - The aim of this work was the application of previously developed and development of new PCR markers for the evaluation of sunflower resistance to downy mildew. Twenty sunflower inbred lines were investigated. Plant resistance to downy mildew was determined by the whole seedling immersion method. Genomic DNA was extracted from the first pair of leaves, and its polymorphism was investigated by RAPD, SSR and several published markers for disease resistance. The presence of the markers Ha-NBS 7/R, Ha-NBS 8/R Ha-NBS 9/R, in Pl6 donor lines (Ha-335, JM-8) and in resistant progeny (Ha-26, G12, G10, G11) confirm that HAP3 could be useful for the detection of the Pl6 gene. DNA polymorphism, which coincided with disease resistance, was revealed with one RAPD (UBC 119 fragment 900-1000 bp) and one SSR primer (ORS37 fragment 600-700 bp). Amplified fragments segregated in the same way i.e., they appeared in 50% of the resistant genotypes. The non-expecting SSR fragment was purified, cloned and sequenced. The results indicated that this fragment is not a part of a coding sequence. Specific primers for the amplification of this fragment have been designed and the investigation of the inheritance of this SCAR marker is under way. None of the applied markers appeared in all resistant genotypes. In order to select lines for making crosses for use in further investigation, the obtained results were also used for the calculation of genetic distances between genotypes (simple matching coefficient) and the construction of a dendrogram (UPGMA method). PB - Institut za ratarstvo i povrtarstvo, Novi Sad T2 - Helia T1 - The use of PCR-based markers in the evaluation of resistance to downy mildew in ns-breeding material EP - 158 IS - 40 SP - 149 VL - 27 DO - 10.2298/HEL0440149P ER -
@article{ author = "Panković, Dejana and Jocić, Siniša and Lačok, Nada and Sakač, Zvonimir and Škorić, Dragan", year = "2004", abstract = "The aim of this work was the application of previously developed and development of new PCR markers for the evaluation of sunflower resistance to downy mildew. Twenty sunflower inbred lines were investigated. Plant resistance to downy mildew was determined by the whole seedling immersion method. Genomic DNA was extracted from the first pair of leaves, and its polymorphism was investigated by RAPD, SSR and several published markers for disease resistance. The presence of the markers Ha-NBS 7/R, Ha-NBS 8/R Ha-NBS 9/R, in Pl6 donor lines (Ha-335, JM-8) and in resistant progeny (Ha-26, G12, G10, G11) confirm that HAP3 could be useful for the detection of the Pl6 gene. DNA polymorphism, which coincided with disease resistance, was revealed with one RAPD (UBC 119 fragment 900-1000 bp) and one SSR primer (ORS37 fragment 600-700 bp). Amplified fragments segregated in the same way i.e., they appeared in 50% of the resistant genotypes. The non-expecting SSR fragment was purified, cloned and sequenced. The results indicated that this fragment is not a part of a coding sequence. Specific primers for the amplification of this fragment have been designed and the investigation of the inheritance of this SCAR marker is under way. None of the applied markers appeared in all resistant genotypes. In order to select lines for making crosses for use in further investigation, the obtained results were also used for the calculation of genetic distances between genotypes (simple matching coefficient) and the construction of a dendrogram (UPGMA method).", publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad", journal = "Helia", title = "The use of PCR-based markers in the evaluation of resistance to downy mildew in ns-breeding material", pages = "158-149", number = "40", volume = "27", doi = "10.2298/HEL0440149P" }
Panković, D., Jocić, S., Lačok, N., Sakač, Z.,& Škorić, D.. (2004). The use of PCR-based markers in the evaluation of resistance to downy mildew in ns-breeding material. in Helia Institut za ratarstvo i povrtarstvo, Novi Sad., 27(40), 149-158. https://doi.org/10.2298/HEL0440149P
Panković D, Jocić S, Lačok N, Sakač Z, Škorić D. The use of PCR-based markers in the evaluation of resistance to downy mildew in ns-breeding material. in Helia. 2004;27(40):149-158. doi:10.2298/HEL0440149P .
Panković, Dejana, Jocić, Siniša, Lačok, Nada, Sakač, Zvonimir, Škorić, Dragan, "The use of PCR-based markers in the evaluation of resistance to downy mildew in ns-breeding material" in Helia, 27, no. 40 (2004):149-158, https://doi.org/10.2298/HEL0440149P . .