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Molecular and serological identification of Pseudomonas syringae pv. tomato in commercial tomato seeds

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2007
bitstream_7200.pdf (1.383Mb)
Authors
Ignjatov, Maja
Milošević, Mirjana
Petrović, Dragana
Medić-Pap, Slađana
Conference object (Published version)
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Abstract
The bacterium Pseudomonas syringae pv. tomato is seedborne pathogen, causal agent of bacterial speck disease on tomatoes. Frequent appearance of disease was recorded in growing areas in Serbia (Vojvodina province). Symptoms may cause lesions on fruits deep enough to reduce quality of tomatoes. Infected tomato seed is the most significant source of inoculum, and healthy seed is the best measure for disease prevention. Seeds were collected from several major growing areas of tomato in Vojvodina. A study was carried out to determine presence of this pathogen on commercial tomato seeds using serological and molecular methods of identification. Isolation of pathogen was done according to NSHS CDFA Method So 5.1. Chosen isolates were used for further investigation including referent strain of P. s. pv. tomato (KFB 145), Agricultural faculty, Zemun. Bacterium identification was done using serological method (ELISA test) with appropriate antibodies of Pseudomonas syringae pv. tomato NEOGEN Eu...rope Ltd., Scotland, UK. Molecular technique (PCR) was also used for detection of bacterium (coronatine producing strains) in tomato seeds.

Keywords:
Pseudomonas syringae pv. tomato / diseases / tomato / Serbia / seeds
Source:
Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007, 2007, 135-135
Publisher:
  • Leuven : International Society for Horticultural Science
[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_fiver_2605
URI
http://fiver.ifvcns.rs/handle/123456789/2605
Collections
  • Radovi istraživača / Researchers' papers
Institution/Community
FiVeR
TY  - CONF
AU  - Ignjatov, Maja
AU  - Milošević, Mirjana
AU  - Petrović, Dragana
AU  - Medić-Pap, Slađana
PY  - 2007
UR  - http://fiver.ifvcns.rs/handle/123456789/2605
AB  - The bacterium Pseudomonas syringae pv.  tomato is seedborne pathogen, causal agent of bacterial speck disease on tomatoes. Frequent appearance of disease was recorded in growing areas in Serbia (Vojvodina province). Symptoms may cause lesions on fruits deep enough to reduce quality of tomatoes. Infected tomato seed is the most significant source of inoculum, and healthy seed is the best measure for disease prevention. Seeds were collected from several major growing areas of tomato in Vojvodina. A study was carried out to determine presence of this pathogen on commercial tomato seeds using serological and molecular methods of identification. Isolation of pathogen was done according to NSHS CDFA Method So 5.1. Chosen isolates were used for further investigation including referent strain of P. s. pv. tomato (KFB 145), Agricultural faculty, Zemun. Bacterium identification was done using serological method (ELISA test) with appropriate antibodies of Pseudomonas syringae pv. tomato NEOGEN Europe Ltd., Scotland, UK. Molecular technique (PCR) was also used for detection of bacterium (coronatine producing strains) in tomato seeds.
PB  - Leuven : International Society for Horticultural Science
C3  - Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007
T1  - Molecular and serological identification of Pseudomonas syringae pv. tomato in commercial tomato seeds
EP  - 135
SP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2605
ER  - 
@conference{
author = "Ignjatov, Maja and Milošević, Mirjana and Petrović, Dragana and Medić-Pap, Slađana",
year = "2007",
abstract = "The bacterium Pseudomonas syringae pv.  tomato is seedborne pathogen, causal agent of bacterial speck disease on tomatoes. Frequent appearance of disease was recorded in growing areas in Serbia (Vojvodina province). Symptoms may cause lesions on fruits deep enough to reduce quality of tomatoes. Infected tomato seed is the most significant source of inoculum, and healthy seed is the best measure for disease prevention. Seeds were collected from several major growing areas of tomato in Vojvodina. A study was carried out to determine presence of this pathogen on commercial tomato seeds using serological and molecular methods of identification. Isolation of pathogen was done according to NSHS CDFA Method So 5.1. Chosen isolates were used for further investigation including referent strain of P. s. pv. tomato (KFB 145), Agricultural faculty, Zemun. Bacterium identification was done using serological method (ELISA test) with appropriate antibodies of Pseudomonas syringae pv. tomato NEOGEN Europe Ltd., Scotland, UK. Molecular technique (PCR) was also used for detection of bacterium (coronatine producing strains) in tomato seeds.",
publisher = "Leuven : International Society for Horticultural Science",
journal = "Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007",
title = "Molecular and serological identification of Pseudomonas syringae pv. tomato in commercial tomato seeds",
pages = "135-135",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2605"
}
Ignjatov, M., Milošević, M., Petrović, D.,& Medić-Pap, S.. (2007). Molecular and serological identification of Pseudomonas syringae pv. tomato in commercial tomato seeds. in Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007
Leuven : International Society for Horticultural Science., 135-135.
https://hdl.handle.net/21.15107/rcub_fiver_2605
Ignjatov M, Milošević M, Petrović D, Medić-Pap S. Molecular and serological identification of Pseudomonas syringae pv. tomato in commercial tomato seeds. in Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007. 2007;:135-135.
https://hdl.handle.net/21.15107/rcub_fiver_2605 .
Ignjatov, Maja, Milošević, Mirjana, Petrović, Dragana, Medić-Pap, Slađana, "Molecular and serological identification of Pseudomonas syringae pv. tomato in commercial tomato seeds" in Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007 (2007):135-135,
https://hdl.handle.net/21.15107/rcub_fiver_2605 .

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