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First report of Fusarium oxysporum f. sp. ciceris on chickpea (Cicer arietinum L.) in Serbia

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2022
pdis-09-21-1998-pdn.pdf (100.3Kb)
Authors
Živanov, Dalibor
Tančić-Živanov, Sonja
Savić, Aleksandra
Uhlarik, Ana
Mamlić, Zlatica
Medić-Pap, Slađana
Nagl, Nevena
Article (Published version)
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Abstract
Chickpea (Cicer arietinum L.) is one of the most commonly consumed legume crops worldwide However, in Serbia, it is a novel crop grown on approximately 120 acres. Fusarium wilt caused by F. oxysporum f. sp. ciceris is one of the most economically important disease in the most chickpea-growing areas, but yet there has been no formal report of the Fusarium wilt of chickpea in Serbia. In June 2018, the first symptoms of Fusarium wilt were registered as wilted chickpea plants grouped in patches on approximately 5% plants in the area of 3 acres. Symptoms as yellowing and necrosis of foliage appeared as late wilt in the podding stage. Roots of affected plants showed no external discoloration, but cross-sections showed darkbrown discoloration of xylem tissue. To isolate the causal agent, symptomatic plants were collected, and ten cuttings of symptomatic root tissue were surface disinfected with 2% sodium hypochlorite solution for 5 min, rinsed three times in sterile distilled water, air-dried... on sterilized filter paper and plated on potato dextrose agar (PDA) and water agar (WA) amended with streptomycin sulfate. After seven days of incubation, isolates were preliminarily identified according to their morphological characters and Fusarium Laboratory Manual. For each isolate 100, micro/macroconidia and chlamydospores were measured. Conidia were hyaline; macroconidia sickle-shaped, with blunt ends, 2-4 septa (10.1-17.7 x 3.1-5.8 μm); microconidia ellipsoidal, 0-1 septa (4.9-8.6-2.7-3.5 μm). Chlamydospores were globose (4.3-8.8 μm). Representative isolates (K343, K375 and K378) were purified by a single-spore technique for further analyses (Leslie and Summerell, 2006). Molecular identification of three representative isolates (K343, K375 and K378) was made by sequencing the rRNA internal transcribed spacer (ITS) region and translation elongation factor 1α (TEF1) gen. For all isolates, the ITS and TEF1 genes were amplified and sequenced with primers ITS1/4 EF1-728 and EF1-986. Based on a BLAST search of the NCBI nucleotide database, the ITS sequences (GenBank MK920204.1, MK928423.1 and MK928424.1) had 99.8% identity with F. oxysporum f. sp. ciceris isolate (MK074845.1). The TEF1 (GenBank MN788462.1, MN788463.1 and MN788464.1) had 96.3 -100% identities with F. oxysporum f. sp. ciceris isolate (FJ538245.1). The pathogenicity test was conducted on 7-day old plants according to the drench method. The concentration of inoculation suspensions was adjusted on 1x106, and ten plants per isolate were tested and inoculated with 10 ml of suspension. Control plants were drenched with 10 ml of sterilized distilled water. Nine days after inoculation, first symptoms as leaves wilting and white mycelia presence around stem base occurred on plants inoculated with isolate K378. On day 11, the first symptoms occurred on plants inoculated with isolates K343 and K375 as well. Up to the 13th day after inoculation, all plants were wilted, and the pathogen was successfully reisolated and confirmed as F. oxysporum f.sp. ciceris. To the best of our knowledge, this is the first report of F. oxysporum f.sp. ciceris causing Fusarium wilt on chickpea in Serbia.

Keywords:
Fusarium oxysporum / chickpea / symptomatic plants / chlamydospores / fusarium wilt / Serbia
Source:
Plant Disease, 2022, 106, 5, 1530-
Publisher:
  • American Phytopathological Society
Funding / projects:
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200032 (Institute of Field and Vegetable Crops, Novi Sad) (RS-200032)

DOI: 10.1094/PDIS-09-21-1998-PDN

ISSN: 0191-2917

WoS: 00078686640000

Scopus: 2-s2.0-85129583209
[ Google Scholar ]
URI
http://fiver.ifvcns.rs/handle/123456789/2525
Collections
  • Radovi istraživača / Researchers' papers
Institution/Community
FiVeR
TY  - JOUR
AU  - Živanov, Dalibor
AU  - Tančić-Živanov, Sonja
AU  - Savić, Aleksandra
AU  - Uhlarik, Ana
AU  - Mamlić, Zlatica
AU  - Medić-Pap, Slađana
AU  - Nagl, Nevena
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2525
AB  - Chickpea (Cicer arietinum L.) is one of the most commonly consumed legume crops worldwide However, in Serbia, it is a novel crop grown on approximately 120 acres. Fusarium wilt caused by F. oxysporum f. sp. ciceris is one of the most economically important disease in the most chickpea-growing areas, but yet there has been no formal report of the Fusarium wilt of chickpea in Serbia. In June 2018, the first symptoms of Fusarium wilt were registered as wilted chickpea plants grouped in patches on approximately 5% plants in the area of 3 acres. Symptoms as yellowing and necrosis of foliage appeared as late wilt in the podding stage. Roots of affected plants showed no external discoloration, but cross-sections showed darkbrown discoloration of xylem tissue. To isolate the causal agent, symptomatic plants were collected, and ten cuttings of symptomatic root tissue were surface disinfected with 2% sodium hypochlorite solution for 5 min, rinsed three times in sterile distilled water, air-dried on sterilized filter paper and plated on potato dextrose agar (PDA) and water agar (WA) amended with streptomycin sulfate. After seven days of incubation, isolates were preliminarily identified according to their morphological characters and Fusarium Laboratory Manual. For each isolate 100, micro/macroconidia and chlamydospores were measured. Conidia were hyaline; macroconidia sickle-shaped, with blunt ends, 2-4 septa (10.1-17.7 x 3.1-5.8 μm); microconidia ellipsoidal, 0-1 septa (4.9-8.6-2.7-3.5 μm). Chlamydospores were globose (4.3-8.8 μm). Representative isolates (K343, K375 and K378) were purified by a single-spore technique for further analyses (Leslie and Summerell, 2006). Molecular identification of three representative isolates (K343, K375 and K378) was made by sequencing the rRNA internal transcribed spacer (ITS) region and translation elongation factor 1α (TEF1) gen. For all isolates, the ITS and TEF1 genes were amplified and sequenced with primers ITS1/4 EF1-728 and EF1-986. Based on a BLAST search of the NCBI nucleotide database, the ITS sequences (GenBank MK920204.1, MK928423.1 and MK928424.1) had 99.8% identity with F. oxysporum f. sp. ciceris isolate (MK074845.1). The TEF1 (GenBank MN788462.1, MN788463.1 and MN788464.1) had 96.3 -100% identities with F. oxysporum f. sp. ciceris isolate (FJ538245.1). The pathogenicity test was conducted on 7-day old plants according to the drench method. The concentration of inoculation suspensions was adjusted on 1x106, and ten plants per isolate were tested and inoculated with 10 ml of suspension. Control plants were drenched with 10 ml of sterilized distilled water. Nine days after inoculation, first symptoms as leaves wilting and white mycelia presence around stem base occurred on plants inoculated with isolate K378. On day 11, the first symptoms occurred on plants inoculated with isolates K343 and K375 as well. Up to the 13th day after inoculation, all plants were wilted, and the pathogen was successfully reisolated and confirmed as F. oxysporum f.sp. ciceris. To the best of our knowledge, this is the first report of F. oxysporum f.sp. ciceris causing Fusarium wilt on chickpea in Serbia.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - First report of Fusarium oxysporum f. sp. ciceris on chickpea (Cicer arietinum L.) in Serbia
IS  - 5
SP  - 1530
VL  - 106
DO  - 10.1094/PDIS-09-21-1998-PDN
ER  - 
@article{
author = "Živanov, Dalibor and Tančić-Živanov, Sonja and Savić, Aleksandra and Uhlarik, Ana and Mamlić, Zlatica and Medić-Pap, Slađana and Nagl, Nevena",
year = "2022",
abstract = "Chickpea (Cicer arietinum L.) is one of the most commonly consumed legume crops worldwide However, in Serbia, it is a novel crop grown on approximately 120 acres. Fusarium wilt caused by F. oxysporum f. sp. ciceris is one of the most economically important disease in the most chickpea-growing areas, but yet there has been no formal report of the Fusarium wilt of chickpea in Serbia. In June 2018, the first symptoms of Fusarium wilt were registered as wilted chickpea plants grouped in patches on approximately 5% plants in the area of 3 acres. Symptoms as yellowing and necrosis of foliage appeared as late wilt in the podding stage. Roots of affected plants showed no external discoloration, but cross-sections showed darkbrown discoloration of xylem tissue. To isolate the causal agent, symptomatic plants were collected, and ten cuttings of symptomatic root tissue were surface disinfected with 2% sodium hypochlorite solution for 5 min, rinsed three times in sterile distilled water, air-dried on sterilized filter paper and plated on potato dextrose agar (PDA) and water agar (WA) amended with streptomycin sulfate. After seven days of incubation, isolates were preliminarily identified according to their morphological characters and Fusarium Laboratory Manual. For each isolate 100, micro/macroconidia and chlamydospores were measured. Conidia were hyaline; macroconidia sickle-shaped, with blunt ends, 2-4 septa (10.1-17.7 x 3.1-5.8 μm); microconidia ellipsoidal, 0-1 septa (4.9-8.6-2.7-3.5 μm). Chlamydospores were globose (4.3-8.8 μm). Representative isolates (K343, K375 and K378) were purified by a single-spore technique for further analyses (Leslie and Summerell, 2006). Molecular identification of three representative isolates (K343, K375 and K378) was made by sequencing the rRNA internal transcribed spacer (ITS) region and translation elongation factor 1α (TEF1) gen. For all isolates, the ITS and TEF1 genes were amplified and sequenced with primers ITS1/4 EF1-728 and EF1-986. Based on a BLAST search of the NCBI nucleotide database, the ITS sequences (GenBank MK920204.1, MK928423.1 and MK928424.1) had 99.8% identity with F. oxysporum f. sp. ciceris isolate (MK074845.1). The TEF1 (GenBank MN788462.1, MN788463.1 and MN788464.1) had 96.3 -100% identities with F. oxysporum f. sp. ciceris isolate (FJ538245.1). The pathogenicity test was conducted on 7-day old plants according to the drench method. The concentration of inoculation suspensions was adjusted on 1x106, and ten plants per isolate were tested and inoculated with 10 ml of suspension. Control plants were drenched with 10 ml of sterilized distilled water. Nine days after inoculation, first symptoms as leaves wilting and white mycelia presence around stem base occurred on plants inoculated with isolate K378. On day 11, the first symptoms occurred on plants inoculated with isolates K343 and K375 as well. Up to the 13th day after inoculation, all plants were wilted, and the pathogen was successfully reisolated and confirmed as F. oxysporum f.sp. ciceris. To the best of our knowledge, this is the first report of F. oxysporum f.sp. ciceris causing Fusarium wilt on chickpea in Serbia.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "First report of Fusarium oxysporum f. sp. ciceris on chickpea (Cicer arietinum L.) in Serbia",
number = "5",
pages = "1530",
volume = "106",
doi = "10.1094/PDIS-09-21-1998-PDN"
}
Živanov, D., Tančić-Živanov, S., Savić, A., Uhlarik, A., Mamlić, Z., Medić-Pap, S.,& Nagl, N.. (2022). First report of Fusarium oxysporum f. sp. ciceris on chickpea (Cicer arietinum L.) in Serbia. in Plant Disease
American Phytopathological Society., 106(5), 1530.
https://doi.org/10.1094/PDIS-09-21-1998-PDN
Živanov D, Tančić-Živanov S, Savić A, Uhlarik A, Mamlić Z, Medić-Pap S, Nagl N. First report of Fusarium oxysporum f. sp. ciceris on chickpea (Cicer arietinum L.) in Serbia. in Plant Disease. 2022;106(5):1530.
doi:10.1094/PDIS-09-21-1998-PDN .
Živanov, Dalibor, Tančić-Živanov, Sonja, Savić, Aleksandra, Uhlarik, Ana, Mamlić, Zlatica, Medić-Pap, Slađana, Nagl, Nevena, "First report of Fusarium oxysporum f. sp. ciceris on chickpea (Cicer arietinum L.) in Serbia" in Plant Disease, 106, no. 5 (2022):1530,
https://doi.org/10.1094/PDIS-09-21-1998-PDN . .

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