Influence of the Macrophomina phaseolina (Tassi) Goid. on SOD activity in four different genotypes of soybean seedlings
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Macrophomina phaseolina (Tassi) Goid. is a very common pest in Serbia, which
affects root and lower stem in many plants, causing charcoal rot. Among other plants,
soybean is also often affected by this pathogen and, like others, on the attack
responds with oxidative burst. The aim of this work was to compare the sensitivity
of four different genotypes of soybean (Glycine max (L.) Merr.): FAVORIT,
ATLAS, SAVA and RUBIN on this pathogen. The activity of antioxidant enzyme
superoxide-dismutase (SOD; EC 1.15.1.1.) was compared between four soybean
genotypes, after the attack of the fungus M. phaseolina. Soybean seeds were
inoculated using the method of artificial inoculation on filter paper. After seven days,
seedlings were collected for biochemical assays. It was measured 1 g of plant
material fresh weight and homogenized with 10 ml 0.1 M K2HPO4, pH 7.0. After
centrifugation, the supernatant was used for enzyme activity measurements. The
SOD activity was measured spectrophotom...etrically, by monitoring the inhibition of
nitroblue tetrazolium (NBT) reduction at 560 nm, according to method by Mandal et
al (2008).
Кључне речи:
Macrophomina phaseolina / oxidative stress / soybean / superoxidedismutase (SOD) / oxidative burst / genotypesИзвор:
Book of Abstracts, 10th International Symposium on Agricultural Sciences “AgroReS 2021", 27-29 May 2021, Trebinje, Bosnia and Herzegovina, 2021, 31-31Издавач:
- Banja Luka : University of Banja Luka, Faculty of Agriculture
Колекције
Институција/група
FiVeRTY - CONF AU - Crnković, Marina AU - Malenčić, Đorđe AU - Petrović, Kristina AU - Šućur, Jovana PY - 2021 UR - http://fiver.ifvcns.rs/handle/123456789/2362 AB - Macrophomina phaseolina (Tassi) Goid. is a very common pest in Serbia, which affects root and lower stem in many plants, causing charcoal rot. Among other plants, soybean is also often affected by this pathogen and, like others, on the attack responds with oxidative burst. The aim of this work was to compare the sensitivity of four different genotypes of soybean (Glycine max (L.) Merr.): FAVORIT, ATLAS, SAVA and RUBIN on this pathogen. The activity of antioxidant enzyme superoxide-dismutase (SOD; EC 1.15.1.1.) was compared between four soybean genotypes, after the attack of the fungus M. phaseolina. Soybean seeds were inoculated using the method of artificial inoculation on filter paper. After seven days, seedlings were collected for biochemical assays. It was measured 1 g of plant material fresh weight and homogenized with 10 ml 0.1 M K2HPO4, pH 7.0. After centrifugation, the supernatant was used for enzyme activity measurements. The SOD activity was measured spectrophotometrically, by monitoring the inhibition of nitroblue tetrazolium (NBT) reduction at 560 nm, according to method by Mandal et al (2008). PB - Banja Luka : University of Banja Luka, Faculty of Agriculture C3 - Book of Abstracts, 10th International Symposium on Agricultural Sciences “AgroReS 2021", 27-29 May 2021, Trebinje, Bosnia and Herzegovina T1 - Influence of the Macrophomina phaseolina (Tassi) Goid. on SOD activity in four different genotypes of soybean seedlings EP - 31 SP - 31 UR - https://hdl.handle.net/21.15107/rcub_fiver_2362 ER -
@conference{ author = "Crnković, Marina and Malenčić, Đorđe and Petrović, Kristina and Šućur, Jovana", year = "2021", abstract = "Macrophomina phaseolina (Tassi) Goid. is a very common pest in Serbia, which affects root and lower stem in many plants, causing charcoal rot. Among other plants, soybean is also often affected by this pathogen and, like others, on the attack responds with oxidative burst. The aim of this work was to compare the sensitivity of four different genotypes of soybean (Glycine max (L.) Merr.): FAVORIT, ATLAS, SAVA and RUBIN on this pathogen. The activity of antioxidant enzyme superoxide-dismutase (SOD; EC 1.15.1.1.) was compared between four soybean genotypes, after the attack of the fungus M. phaseolina. Soybean seeds were inoculated using the method of artificial inoculation on filter paper. After seven days, seedlings were collected for biochemical assays. It was measured 1 g of plant material fresh weight and homogenized with 10 ml 0.1 M K2HPO4, pH 7.0. After centrifugation, the supernatant was used for enzyme activity measurements. The SOD activity was measured spectrophotometrically, by monitoring the inhibition of nitroblue tetrazolium (NBT) reduction at 560 nm, according to method by Mandal et al (2008).", publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture", journal = "Book of Abstracts, 10th International Symposium on Agricultural Sciences “AgroReS 2021", 27-29 May 2021, Trebinje, Bosnia and Herzegovina", title = "Influence of the Macrophomina phaseolina (Tassi) Goid. on SOD activity in four different genotypes of soybean seedlings", pages = "31-31", url = "https://hdl.handle.net/21.15107/rcub_fiver_2362" }
Crnković, M., Malenčić, Đ., Petrović, K.,& Šućur, J.. (2021). Influence of the Macrophomina phaseolina (Tassi) Goid. on SOD activity in four different genotypes of soybean seedlings. in Book of Abstracts, 10th International Symposium on Agricultural Sciences “AgroReS 2021", 27-29 May 2021, Trebinje, Bosnia and Herzegovina Banja Luka : University of Banja Luka, Faculty of Agriculture., 31-31. https://hdl.handle.net/21.15107/rcub_fiver_2362
Crnković M, Malenčić Đ, Petrović K, Šućur J. Influence of the Macrophomina phaseolina (Tassi) Goid. on SOD activity in four different genotypes of soybean seedlings. in Book of Abstracts, 10th International Symposium on Agricultural Sciences “AgroReS 2021", 27-29 May 2021, Trebinje, Bosnia and Herzegovina. 2021;:31-31. https://hdl.handle.net/21.15107/rcub_fiver_2362 .
Crnković, Marina, Malenčić, Đorđe, Petrović, Kristina, Šućur, Jovana, "Influence of the Macrophomina phaseolina (Tassi) Goid. on SOD activity in four different genotypes of soybean seedlings" in Book of Abstracts, 10th International Symposium on Agricultural Sciences “AgroReS 2021", 27-29 May 2021, Trebinje, Bosnia and Herzegovina (2021):31-31, https://hdl.handle.net/21.15107/rcub_fiver_2362 .