Allelopathic effects of industrial hemp (Cannabis sativa L.) on antioxidant enzymes activity of soybean seedlings
Authors
Prvulović, DejanOvuka, Jelena

Gvozdenac, Sonja

Peić Tukuljac, Marijana

Kiprovski, Biljana

Koren, Anamarija

Latković, Dragana
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Fibre or industrial hemp (Cannabis sativa L.) have been cultivated for thousands of years as a fibre, seed or dual-propose crop. Industrial hemp is currently witnessing a revival as a rich source of secondary metabolites. Several studies showed that hemp could be used in pest control. It has been documented that hemp could suppress weeds, harmful nematodes and soil pathogens and also could have allelopathic effects on some field crops (inhibitory or stimulatory). The aim of this study was to examine the effects of ethanol extracts of industrial hemp on activity of antioxidative enzymes, activity of enzymes of polyphenolic metabolism and intensity of lipid peroxidation in
seedlings of soybean (Glycine max (L.) Merr.). This survey was also conducted in order to evaluate the allelopathic activity of hemp extract on content of phenolic compounds and antioxidant capacity of soybean seedlings. In the experiments the 70% ethanol extracts of dried flowering buds of hemp (cv. Helena) was appli...ed in 0.5, 1.0 and 2.0% concentration on seeds
of soybean. Ethanol solution (70%) was used as a control. Experiment was performed according to ISTA recommendations (2017). After eight days plants were harvested and activity of antioxidant enzymes (catalase, superoxide dismutase and peroxidase), enzymes of polyphenolic metabolism (phenylalanine ammonia lyase and polyphenol oxidase) and intensity of lipid peroxidation was measured in phosphate buffer extracts of whole seedlings. Content of total phenolics and tannins and antioxidant capacity was measured in extracts of soybean
seedlings with 70% methanol. Extract of hemp decreased activity of phenylalanine ammonia lyasein soybean seedlings and also decreased content of total phenolic and antioxidant capacity.
Treatments with ethanol extract of firbe hemp did not affect activities of antioxidative enzymes and did not induce lipid peroxidation in soybean seedlings tissues.
Keywords:
hemp / allelopathy / enzymatic activity / antioxidants / polyphenols / lipid peroxidation / seedlings / soybean / extractsSource:
Book of Abstracts, 7th Central European Congress of Life Sciences-Eurobiotech, 23-25.09.2019. Krakow, Poland, 2019, 64-65Publisher:
- Krakow: Targi w Krakowie Ltd.
Funding / projects:
- "Renesansa industrijske konoplje u svetlu aktuelnih agronomsko-medicinskih izazova", ugovor br. 114-451-2178/2016-03, Pokrajinski sekretarijat za visoko obrazovanje i naučnoistraživačku delatnost, Autonomna pokrajina Vojvodina, Republika Srbija (2016-2020)
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FiVeRTY - CONF AU - Prvulović, Dejan AU - Ovuka, Jelena AU - Gvozdenac, Sonja AU - Peić Tukuljac, Marijana AU - Kiprovski, Biljana AU - Koren, Anamarija AU - Latković, Dragana PY - 2019 UR - http://fiver.ifvcns.rs/handle/123456789/2226 AB - Fibre or industrial hemp (Cannabis sativa L.) have been cultivated for thousands of years as a fibre, seed or dual-propose crop. Industrial hemp is currently witnessing a revival as a rich source of secondary metabolites. Several studies showed that hemp could be used in pest control. It has been documented that hemp could suppress weeds, harmful nematodes and soil pathogens and also could have allelopathic effects on some field crops (inhibitory or stimulatory). The aim of this study was to examine the effects of ethanol extracts of industrial hemp on activity of antioxidative enzymes, activity of enzymes of polyphenolic metabolism and intensity of lipid peroxidation in seedlings of soybean (Glycine max (L.) Merr.). This survey was also conducted in order to evaluate the allelopathic activity of hemp extract on content of phenolic compounds and antioxidant capacity of soybean seedlings. In the experiments the 70% ethanol extracts of dried flowering buds of hemp (cv. Helena) was applied in 0.5, 1.0 and 2.0% concentration on seeds of soybean. Ethanol solution (70%) was used as a control. Experiment was performed according to ISTA recommendations (2017). After eight days plants were harvested and activity of antioxidant enzymes (catalase, superoxide dismutase and peroxidase), enzymes of polyphenolic metabolism (phenylalanine ammonia lyase and polyphenol oxidase) and intensity of lipid peroxidation was measured in phosphate buffer extracts of whole seedlings. Content of total phenolics and tannins and antioxidant capacity was measured in extracts of soybean seedlings with 70% methanol. Extract of hemp decreased activity of phenylalanine ammonia lyasein soybean seedlings and also decreased content of total phenolic and antioxidant capacity. Treatments with ethanol extract of firbe hemp did not affect activities of antioxidative enzymes and did not induce lipid peroxidation in soybean seedlings tissues. PB - Krakow: Targi w Krakowie Ltd. C3 - Book of Abstracts, 7th Central European Congress of Life Sciences-Eurobiotech, 23-25.09.2019. Krakow, Poland T1 - Allelopathic effects of industrial hemp (Cannabis sativa L.) on antioxidant enzymes activity of soybean seedlings EP - 65 SP - 64 UR - https://hdl.handle.net/21.15107/rcub_fiver_2226 ER -
@conference{ author = "Prvulović, Dejan and Ovuka, Jelena and Gvozdenac, Sonja and Peić Tukuljac, Marijana and Kiprovski, Biljana and Koren, Anamarija and Latković, Dragana", year = "2019", abstract = "Fibre or industrial hemp (Cannabis sativa L.) have been cultivated for thousands of years as a fibre, seed or dual-propose crop. Industrial hemp is currently witnessing a revival as a rich source of secondary metabolites. Several studies showed that hemp could be used in pest control. It has been documented that hemp could suppress weeds, harmful nematodes and soil pathogens and also could have allelopathic effects on some field crops (inhibitory or stimulatory). The aim of this study was to examine the effects of ethanol extracts of industrial hemp on activity of antioxidative enzymes, activity of enzymes of polyphenolic metabolism and intensity of lipid peroxidation in seedlings of soybean (Glycine max (L.) Merr.). This survey was also conducted in order to evaluate the allelopathic activity of hemp extract on content of phenolic compounds and antioxidant capacity of soybean seedlings. In the experiments the 70% ethanol extracts of dried flowering buds of hemp (cv. Helena) was applied in 0.5, 1.0 and 2.0% concentration on seeds of soybean. Ethanol solution (70%) was used as a control. Experiment was performed according to ISTA recommendations (2017). After eight days plants were harvested and activity of antioxidant enzymes (catalase, superoxide dismutase and peroxidase), enzymes of polyphenolic metabolism (phenylalanine ammonia lyase and polyphenol oxidase) and intensity of lipid peroxidation was measured in phosphate buffer extracts of whole seedlings. Content of total phenolics and tannins and antioxidant capacity was measured in extracts of soybean seedlings with 70% methanol. Extract of hemp decreased activity of phenylalanine ammonia lyasein soybean seedlings and also decreased content of total phenolic and antioxidant capacity. Treatments with ethanol extract of firbe hemp did not affect activities of antioxidative enzymes and did not induce lipid peroxidation in soybean seedlings tissues.", publisher = "Krakow: Targi w Krakowie Ltd.", journal = "Book of Abstracts, 7th Central European Congress of Life Sciences-Eurobiotech, 23-25.09.2019. Krakow, Poland", title = "Allelopathic effects of industrial hemp (Cannabis sativa L.) on antioxidant enzymes activity of soybean seedlings", pages = "65-64", url = "https://hdl.handle.net/21.15107/rcub_fiver_2226" }
Prvulović, D., Ovuka, J., Gvozdenac, S., Peić Tukuljac, M., Kiprovski, B., Koren, A.,& Latković, D.. (2019). Allelopathic effects of industrial hemp (Cannabis sativa L.) on antioxidant enzymes activity of soybean seedlings. in Book of Abstracts, 7th Central European Congress of Life Sciences-Eurobiotech, 23-25.09.2019. Krakow, Poland Krakow: Targi w Krakowie Ltd.., 64-65. https://hdl.handle.net/21.15107/rcub_fiver_2226
Prvulović D, Ovuka J, Gvozdenac S, Peić Tukuljac M, Kiprovski B, Koren A, Latković D. Allelopathic effects of industrial hemp (Cannabis sativa L.) on antioxidant enzymes activity of soybean seedlings. in Book of Abstracts, 7th Central European Congress of Life Sciences-Eurobiotech, 23-25.09.2019. Krakow, Poland. 2019;:64-65. https://hdl.handle.net/21.15107/rcub_fiver_2226 .
Prvulović, Dejan, Ovuka, Jelena, Gvozdenac, Sonja, Peić Tukuljac, Marijana, Kiprovski, Biljana, Koren, Anamarija, Latković, Dragana, "Allelopathic effects of industrial hemp (Cannabis sativa L.) on antioxidant enzymes activity of soybean seedlings" in Book of Abstracts, 7th Central European Congress of Life Sciences-Eurobiotech, 23-25.09.2019. Krakow, Poland (2019):64-65, https://hdl.handle.net/21.15107/rcub_fiver_2226 .