Mitigating lipid peroxidation in leaves of Prunus sp. rootstocks using exogenously applied auxins
Smanjenje intenziteta lipidne peroksidacije u listovima selekcija vegetativnih podloga za višnju i trešnju upotrebom egzogeno primenjenih auksina
Authors
Dorić, DušicaKiprovski, Biljana

Malenčić, Đorđe

Ognjanov, Vladislav
Ljubojević, Mirjana
Barać, Goran

Article (Published version)
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The effect of exogenously applied auxins on mechanical injury induced oxidative stress was studied in 6 promising rootstock selections of Prunus cerasus L., P. mahaleb L., and P. fruticosa Pall. Investigated selections were included in low vigorous rootstock breeding programme for sweet and sour cherries. The standard rootstock PHL-A was used as a control, due to successful rooting. Leaves of investigated rootstocks were collected on: 0, 1st, 3rd and 7th day of inserting softwood cuttings into the rooting substrate under a fogging system with 95-99% average relative humidity. Exogenously applied auxin mixture consisted of 0.8% α-naphthylacetic acid (NAA) and 0.5% indolebutyric acid (IBA). Mechanical injuries during rooting period represent one of the factors that induce stress in softwood cuttings. Intensity of LP is used as a valuable biomarker of plant response to various abiotic factors. Differences in intensity of LP between auxin-treated and -untreated cuttings were examined. Almo...st all of investigated selections had lower LP intensity after auxin application (11.4-47.2%) between 1st and 3rd day. However, the most prominent change was in leaves of PHL-A (49.2%). The best LP-lowering effect were recorded in leaves of P. fruticosa, SV4 selection (56.9%) on 7th day and in P. mahaleb, M4 selection, on 1st and 3rd day (5.9, 5.8%), in comparison to untreated softwood cuttings. As for OV21 selection, LP intensity significantly increased in both treated and untreated cuttings on the 1st, 3rd and 7th day, but auxin-treated cuttings showed lower LP values, except on 7th day, reaching 221.3 nmol MDA equivalents g-1 fresh weight. Intensity of LP during vegetative propagation of selected genotypes could be used as one of the biochemical parameters in further rootstock selection for sweet and sour cherries.
Keywords:
softwood cuttings / auxins / lipid peroxidation / oxidative stress / sweet cherry / zelene reznice / auksini / lipidna peroksidacija / oksidativni stress / trešnjaSource:
Contemporary Agriculture, 2015, 64, 1-2, 78-85Publisher:
- Novi Sad : University of Novi Sad, Faculty of Agriculture
Funding / projects:
- Selection of sweet and sour cherry dwarfing rootstocks and development of intensive cultivation technology based on the sustainable agriculture principles (RS-31038)
- HU-SRB IPA Cross-Border Programme, Project ID: HUSRB/1203/221/173
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FiVeRTY - JOUR AU - Dorić, Dušica AU - Kiprovski, Biljana AU - Malenčić, Đorđe AU - Ognjanov, Vladislav AU - Ljubojević, Mirjana AU - Barać, Goran PY - 2015 UR - http://fiver.ifvcns.rs/handle/123456789/2210 AB - The effect of exogenously applied auxins on mechanical injury induced oxidative stress was studied in 6 promising rootstock selections of Prunus cerasus L., P. mahaleb L., and P. fruticosa Pall. Investigated selections were included in low vigorous rootstock breeding programme for sweet and sour cherries. The standard rootstock PHL-A was used as a control, due to successful rooting. Leaves of investigated rootstocks were collected on: 0, 1st, 3rd and 7th day of inserting softwood cuttings into the rooting substrate under a fogging system with 95-99% average relative humidity. Exogenously applied auxin mixture consisted of 0.8% α-naphthylacetic acid (NAA) and 0.5% indolebutyric acid (IBA). Mechanical injuries during rooting period represent one of the factors that induce stress in softwood cuttings. Intensity of LP is used as a valuable biomarker of plant response to various abiotic factors. Differences in intensity of LP between auxin-treated and -untreated cuttings were examined. Almost all of investigated selections had lower LP intensity after auxin application (11.4-47.2%) between 1st and 3rd day. However, the most prominent change was in leaves of PHL-A (49.2%). The best LP-lowering effect were recorded in leaves of P. fruticosa, SV4 selection (56.9%) on 7th day and in P. mahaleb, M4 selection, on 1st and 3rd day (5.9, 5.8%), in comparison to untreated softwood cuttings. As for OV21 selection, LP intensity significantly increased in both treated and untreated cuttings on the 1st, 3rd and 7th day, but auxin-treated cuttings showed lower LP values, except on 7th day, reaching 221.3 nmol MDA equivalents g-1 fresh weight. Intensity of LP during vegetative propagation of selected genotypes could be used as one of the biochemical parameters in further rootstock selection for sweet and sour cherries. PB - Novi Sad : University of Novi Sad, Faculty of Agriculture T2 - Contemporary Agriculture T1 - Mitigating lipid peroxidation in leaves of Prunus sp. rootstocks using exogenously applied auxins T1 - Smanjenje intenziteta lipidne peroksidacije u listovima selekcija vegetativnih podloga za višnju i trešnju upotrebom egzogeno primenjenih auksina EP - 85 IS - 1-2 SP - 78 VL - 64 UR - https://hdl.handle.net/21.15107/rcub_fiver_2210 ER -
@article{ author = "Dorić, Dušica and Kiprovski, Biljana and Malenčić, Đorđe and Ognjanov, Vladislav and Ljubojević, Mirjana and Barać, Goran", year = "2015", abstract = "The effect of exogenously applied auxins on mechanical injury induced oxidative stress was studied in 6 promising rootstock selections of Prunus cerasus L., P. mahaleb L., and P. fruticosa Pall. Investigated selections were included in low vigorous rootstock breeding programme for sweet and sour cherries. The standard rootstock PHL-A was used as a control, due to successful rooting. Leaves of investigated rootstocks were collected on: 0, 1st, 3rd and 7th day of inserting softwood cuttings into the rooting substrate under a fogging system with 95-99% average relative humidity. Exogenously applied auxin mixture consisted of 0.8% α-naphthylacetic acid (NAA) and 0.5% indolebutyric acid (IBA). Mechanical injuries during rooting period represent one of the factors that induce stress in softwood cuttings. Intensity of LP is used as a valuable biomarker of plant response to various abiotic factors. Differences in intensity of LP between auxin-treated and -untreated cuttings were examined. Almost all of investigated selections had lower LP intensity after auxin application (11.4-47.2%) between 1st and 3rd day. However, the most prominent change was in leaves of PHL-A (49.2%). The best LP-lowering effect were recorded in leaves of P. fruticosa, SV4 selection (56.9%) on 7th day and in P. mahaleb, M4 selection, on 1st and 3rd day (5.9, 5.8%), in comparison to untreated softwood cuttings. As for OV21 selection, LP intensity significantly increased in both treated and untreated cuttings on the 1st, 3rd and 7th day, but auxin-treated cuttings showed lower LP values, except on 7th day, reaching 221.3 nmol MDA equivalents g-1 fresh weight. Intensity of LP during vegetative propagation of selected genotypes could be used as one of the biochemical parameters in further rootstock selection for sweet and sour cherries.", publisher = "Novi Sad : University of Novi Sad, Faculty of Agriculture", journal = "Contemporary Agriculture", title = "Mitigating lipid peroxidation in leaves of Prunus sp. rootstocks using exogenously applied auxins, Smanjenje intenziteta lipidne peroksidacije u listovima selekcija vegetativnih podloga za višnju i trešnju upotrebom egzogeno primenjenih auksina", pages = "85-78", number = "1-2", volume = "64", url = "https://hdl.handle.net/21.15107/rcub_fiver_2210" }
Dorić, D., Kiprovski, B., Malenčić, Đ., Ognjanov, V., Ljubojević, M.,& Barać, G.. (2015). Mitigating lipid peroxidation in leaves of Prunus sp. rootstocks using exogenously applied auxins. in Contemporary Agriculture Novi Sad : University of Novi Sad, Faculty of Agriculture., 64(1-2), 78-85. https://hdl.handle.net/21.15107/rcub_fiver_2210
Dorić D, Kiprovski B, Malenčić Đ, Ognjanov V, Ljubojević M, Barać G. Mitigating lipid peroxidation in leaves of Prunus sp. rootstocks using exogenously applied auxins. in Contemporary Agriculture. 2015;64(1-2):78-85. https://hdl.handle.net/21.15107/rcub_fiver_2210 .
Dorić, Dušica, Kiprovski, Biljana, Malenčić, Đorđe, Ognjanov, Vladislav, Ljubojević, Mirjana, Barać, Goran, "Mitigating lipid peroxidation in leaves of Prunus sp. rootstocks using exogenously applied auxins" in Contemporary Agriculture, 64, no. 1-2 (2015):78-85, https://hdl.handle.net/21.15107/rcub_fiver_2210 .