Host-specific competitiveness to form nodules in Rhizobium leguminosarum symbiovar viciae

2020
Authors
Boivin, StephaneLahmidi, Nassima Ait
Sherlock, David
Bonhomme, Maxime

Dijon, Doriane
Heulin-Gotty, Karine
Le-Quere, Antoine

Pervent, Marjorie
Tauzin, Marc
Carlsson, Georg

Jensen, Erik
Journet, Etienne-Pascal

Lopez-Bellido, Raphael
Seidenglanz, Marek
Marinković, Jelena

Colella, Stefano

Brunel, Brigitte
Young, Peter
Lepetit, Marc
Article (Published version)
Metadata
Show full item recordAbstract
Fabeae legumes such as pea and faba bean form symbiotic nodules with a large diversity of soil Rhizobium leguminosarum symbiovar viciae (Rlv) bacteria. However, bacteria competitive to form root nodules (CFN) are generally not the most efficient to fix dinitrogen, resulting in a decrease in legume crop yields. Here, we investigate differential selection by host plants on the diversity of Rlv. A large collection of Rlv was collected by nodule trapping with pea and faba bean from soils at five European sites. Representative genomes were sequenced. In parallel, diversity and abundance of Rlv were estimated directly in these soils using metabarcoding. The CFN of isolates was measured with both legume hosts. Pea/faba bean CFN were associated to Rlv genomic regions. Variations of bacterial pea and/or faba bean CFN explained the differential abundance of Rlv genotypes in pea and faba bean nodules. No evidence was found for genetic association between CFN and variations in the core genome, but... variations in specific regions of the nod locus, as well as in other plasmid loci, were associated with differences in CFN. These findings shed light on the genetic control of CFN in Rlv and emphasise the importance of host plants in controlling Rhizobium diversity.
Keywords:
competitiveness / genospecies / nod genes / nodules / Pisum sativum / Rhizobium leguminosarum symbiovar viciae / symbiosis / Vicia fabaSource:
New Phytologist, 2020, 226, 2, 555-568Publisher:
- Wiley, Hoboken
Funding / projects:
- LEGumes for the Agriculture of TOmorrow (EU-613551)
- GrasP grant of the Agence Nationale de la RechercheFrench National Research Agency (ANR)
DOI: 10.1111/nph.16392
ISSN: 0028-646X
PubMed: 31873949
WoS: 000509624700001
Scopus: 2-s2.0-85078673270
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FiVeRTY - JOUR AU - Boivin, Stephane AU - Lahmidi, Nassima Ait AU - Sherlock, David AU - Bonhomme, Maxime AU - Dijon, Doriane AU - Heulin-Gotty, Karine AU - Le-Quere, Antoine AU - Pervent, Marjorie AU - Tauzin, Marc AU - Carlsson, Georg AU - Jensen, Erik AU - Journet, Etienne-Pascal AU - Lopez-Bellido, Raphael AU - Seidenglanz, Marek AU - Marinković, Jelena AU - Colella, Stefano AU - Brunel, Brigitte AU - Young, Peter AU - Lepetit, Marc PY - 2020 UR - http://fiver.ifvcns.rs/handle/123456789/1993 AB - Fabeae legumes such as pea and faba bean form symbiotic nodules with a large diversity of soil Rhizobium leguminosarum symbiovar viciae (Rlv) bacteria. However, bacteria competitive to form root nodules (CFN) are generally not the most efficient to fix dinitrogen, resulting in a decrease in legume crop yields. Here, we investigate differential selection by host plants on the diversity of Rlv. A large collection of Rlv was collected by nodule trapping with pea and faba bean from soils at five European sites. Representative genomes were sequenced. In parallel, diversity and abundance of Rlv were estimated directly in these soils using metabarcoding. The CFN of isolates was measured with both legume hosts. Pea/faba bean CFN were associated to Rlv genomic regions. Variations of bacterial pea and/or faba bean CFN explained the differential abundance of Rlv genotypes in pea and faba bean nodules. No evidence was found for genetic association between CFN and variations in the core genome, but variations in specific regions of the nod locus, as well as in other plasmid loci, were associated with differences in CFN. These findings shed light on the genetic control of CFN in Rlv and emphasise the importance of host plants in controlling Rhizobium diversity. PB - Wiley, Hoboken T2 - New Phytologist T1 - Host-specific competitiveness to form nodules in Rhizobium leguminosarum symbiovar viciae EP - 568 IS - 2 SP - 555 VL - 226 DO - 10.1111/nph.16392 ER -
@article{ author = "Boivin, Stephane and Lahmidi, Nassima Ait and Sherlock, David and Bonhomme, Maxime and Dijon, Doriane and Heulin-Gotty, Karine and Le-Quere, Antoine and Pervent, Marjorie and Tauzin, Marc and Carlsson, Georg and Jensen, Erik and Journet, Etienne-Pascal and Lopez-Bellido, Raphael and Seidenglanz, Marek and Marinković, Jelena and Colella, Stefano and Brunel, Brigitte and Young, Peter and Lepetit, Marc", year = "2020", abstract = "Fabeae legumes such as pea and faba bean form symbiotic nodules with a large diversity of soil Rhizobium leguminosarum symbiovar viciae (Rlv) bacteria. However, bacteria competitive to form root nodules (CFN) are generally not the most efficient to fix dinitrogen, resulting in a decrease in legume crop yields. Here, we investigate differential selection by host plants on the diversity of Rlv. A large collection of Rlv was collected by nodule trapping with pea and faba bean from soils at five European sites. Representative genomes were sequenced. In parallel, diversity and abundance of Rlv were estimated directly in these soils using metabarcoding. The CFN of isolates was measured with both legume hosts. Pea/faba bean CFN were associated to Rlv genomic regions. Variations of bacterial pea and/or faba bean CFN explained the differential abundance of Rlv genotypes in pea and faba bean nodules. No evidence was found for genetic association between CFN and variations in the core genome, but variations in specific regions of the nod locus, as well as in other plasmid loci, were associated with differences in CFN. These findings shed light on the genetic control of CFN in Rlv and emphasise the importance of host plants in controlling Rhizobium diversity.", publisher = "Wiley, Hoboken", journal = "New Phytologist", title = "Host-specific competitiveness to form nodules in Rhizobium leguminosarum symbiovar viciae", pages = "568-555", number = "2", volume = "226", doi = "10.1111/nph.16392" }
Boivin, S., Lahmidi, N. A., Sherlock, D., Bonhomme, M., Dijon, D., Heulin-Gotty, K., Le-Quere, A., Pervent, M., Tauzin, M., Carlsson, G., Jensen, E., Journet, E., Lopez-Bellido, R., Seidenglanz, M., Marinković, J., Colella, S., Brunel, B., Young, P.,& Lepetit, M.. (2020). Host-specific competitiveness to form nodules in Rhizobium leguminosarum symbiovar viciae. in New Phytologist Wiley, Hoboken., 226(2), 555-568. https://doi.org/10.1111/nph.16392
Boivin S, Lahmidi NA, Sherlock D, Bonhomme M, Dijon D, Heulin-Gotty K, Le-Quere A, Pervent M, Tauzin M, Carlsson G, Jensen E, Journet E, Lopez-Bellido R, Seidenglanz M, Marinković J, Colella S, Brunel B, Young P, Lepetit M. Host-specific competitiveness to form nodules in Rhizobium leguminosarum symbiovar viciae. in New Phytologist. 2020;226(2):555-568. doi:10.1111/nph.16392 .
Boivin, Stephane, Lahmidi, Nassima Ait, Sherlock, David, Bonhomme, Maxime, Dijon, Doriane, Heulin-Gotty, Karine, Le-Quere, Antoine, Pervent, Marjorie, Tauzin, Marc, Carlsson, Georg, Jensen, Erik, Journet, Etienne-Pascal, Lopez-Bellido, Raphael, Seidenglanz, Marek, Marinković, Jelena, Colella, Stefano, Brunel, Brigitte, Young, Peter, Lepetit, Marc, "Host-specific competitiveness to form nodules in Rhizobium leguminosarum symbiovar viciae" in New Phytologist, 226, no. 2 (2020):555-568, https://doi.org/10.1111/nph.16392 . .