FiVeR - Repository of the Institute of Field and Vegetable Crops
Institute of Field and Vegetable Crops
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   FiVeR
  • FiVeR
  • Radovi istraživača / Researchers' papers
  • View Item
  •   FiVeR
  • FiVeR
  • Radovi istraživača / Researchers' papers
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

A rapid test for detection of tribenuron-methyl resistance in sunflower

Brzi test za ispitivanje otpornosti suncokreta na tribenuron metil

Thumbnail
2016
1523.pdf (1.668Mb)
Authors
Dimitrijević, Aleksandra
Imerovski, Ivana
Miladinović, Dragana
Jocić, Siniša
Cvejić, Sandra
Malidža, Goran
Kovačević, Branislav
Šurlan-Momirović, Gordana
Article (Published version)
Metadata
Show full item record
Abstract
A rapid laboratory test for detection of tribenuron-methyl resistant sunflower genotypes was developed. Four homozygous and four heterozygous tribenuron-methyl resistant hybrids, as well as a susceptible one were grown on MS medium supplemented with different concentrations of herbicide (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM and 4.0 µM) and with pH either 7 or 8. The effect of medium pH and herbicide concentration on above-ground part and root mass of sunflower seedlings was observed. The test enabled visual discrimination between resistant and susceptible genotypes in 5 days, and discrimination between homozygous and heterozygous resistant genotypes in 12 days. All tested herbicide concentrations and morphological parameters were found to be suitable for discrimination of tribenuron-methyl susceptible genotypes. The best pH and herbicide concentration combination and morphological parameter for discrimination between homozygous and heterozygous resistant sunflower genotypes were 3.0 µM at pH... 7 and root mass, respectively.

Razvijen je brzi laboratorijski test za detekciju genotipova suncokreta otpornih na tribenuron metil. Po četiri hibrida suncokreta homozigotno i heterozigotno otporna na tribenuron metil, kao i jedan neotporan hibrid su gajeni na MS podlozi sa pH vrednošću ili 7 ili 8 i sa različitim koncentracijama herbicida Express 50X, čija je aktivna materija tribenuron-metil (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM i 4.0 µM). Ispitivan je uticaj pH i koncentracije herbicida u podlozi na masu nadzemnog dela i korena klijanaca testiranih hibrida. Vizuelne razlike između otpornih i neotpornog hibrida su uočene nakon 5 dana kulture, dok su se razlike između homozigotnih i heterozigotnih otpornih hibrida pojavile nakon 12 dana kulture. Sve testirane koncentracije herbicida i testirani morfološki parametri su se pokazali kao dobri za identifikaciju neotpornih genotipova. Najbolja kombinacija pH i koncentracije herbicida za razlikovanje homozigotnih od heterozigotnih otpornih genotipova je bila 3.0 µM na pH 7, a ...morfološki parametar masa korena.

Keywords:
bioassay / Helianthus annuus L. / herbicides / selection / sulfonylureas / tolerance / biološka proba / Helianthus annuus L. / herbicidi / selekcija / sulfoniluree / tolerantnost
Source:
Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 2016, 53, 1, 1-8
Publisher:
  • Institut za ratarstvo i povrtarstvo, Novi Sad
Funding / projects:
  • Development of new varieties and production technology improvement of oil crops for different purposes (RS-31025)

DOI: 10.5937/ratpov53-8412

ISSN: 1821-3944

[ Google Scholar ]
URI
http://fiver.ifvcns.rs/handle/123456789/1526
Collections
  • Radovi istraživača / Researchers' papers
Institution/Community
FiVeR
TY  - JOUR
AU  - Dimitrijević, Aleksandra
AU  - Imerovski, Ivana
AU  - Miladinović, Dragana
AU  - Jocić, Siniša
AU  - Cvejić, Sandra
AU  - Malidža, Goran
AU  - Kovačević, Branislav
AU  - Šurlan-Momirović, Gordana
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/1526
AB  - A rapid laboratory test for detection of tribenuron-methyl resistant sunflower genotypes was developed. Four homozygous and four heterozygous tribenuron-methyl resistant hybrids, as well as a susceptible one were grown on MS medium supplemented with different concentrations of herbicide (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM and 4.0 µM) and with pH either 7 or 8. The effect of medium pH and herbicide concentration on above-ground part and root mass of sunflower seedlings was observed. The test enabled visual discrimination between resistant and susceptible genotypes in 5 days, and discrimination between homozygous and heterozygous resistant genotypes in 12 days. All tested herbicide concentrations and morphological parameters were found to be suitable for discrimination of tribenuron-methyl susceptible genotypes. The best pH and herbicide concentration combination and morphological parameter for discrimination between homozygous and heterozygous resistant sunflower genotypes were 3.0 µM at pH 7 and root mass, respectively.
AB  - Razvijen je brzi laboratorijski test za detekciju genotipova suncokreta otpornih na tribenuron metil. Po četiri hibrida suncokreta homozigotno i heterozigotno otporna na tribenuron metil, kao i jedan neotporan hibrid su gajeni na MS podlozi sa pH vrednošću ili 7 ili 8 i sa različitim koncentracijama herbicida Express 50X, čija je aktivna materija tribenuron-metil (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM i 4.0 µM). Ispitivan je uticaj pH i koncentracije herbicida u podlozi na masu nadzemnog dela i korena klijanaca testiranih hibrida. Vizuelne razlike između otpornih i neotpornog hibrida su uočene nakon 5 dana kulture, dok su se razlike između homozigotnih i heterozigotnih otpornih hibrida pojavile nakon 12 dana kulture. Sve testirane koncentracije herbicida i testirani morfološki parametri su se pokazali kao dobri za identifikaciju neotpornih genotipova. Najbolja kombinacija pH i koncentracije herbicida za razlikovanje homozigotnih od heterozigotnih otpornih genotipova je bila 3.0 µM na pH 7, a morfološki parametar masa korena.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - A rapid test for detection of tribenuron-methyl resistance in sunflower
T1  - Brzi test za ispitivanje otpornosti suncokreta na tribenuron metil
EP  - 8
IS  - 1
SP  - 1
VL  - 53
DO  - 10.5937/ratpov53-8412
ER  - 
@article{
author = "Dimitrijević, Aleksandra and Imerovski, Ivana and Miladinović, Dragana and Jocić, Siniša and Cvejić, Sandra and Malidža, Goran and Kovačević, Branislav and Šurlan-Momirović, Gordana",
year = "2016",
abstract = "A rapid laboratory test for detection of tribenuron-methyl resistant sunflower genotypes was developed. Four homozygous and four heterozygous tribenuron-methyl resistant hybrids, as well as a susceptible one were grown on MS medium supplemented with different concentrations of herbicide (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM and 4.0 µM) and with pH either 7 or 8. The effect of medium pH and herbicide concentration on above-ground part and root mass of sunflower seedlings was observed. The test enabled visual discrimination between resistant and susceptible genotypes in 5 days, and discrimination between homozygous and heterozygous resistant genotypes in 12 days. All tested herbicide concentrations and morphological parameters were found to be suitable for discrimination of tribenuron-methyl susceptible genotypes. The best pH and herbicide concentration combination and morphological parameter for discrimination between homozygous and heterozygous resistant sunflower genotypes were 3.0 µM at pH 7 and root mass, respectively., Razvijen je brzi laboratorijski test za detekciju genotipova suncokreta otpornih na tribenuron metil. Po četiri hibrida suncokreta homozigotno i heterozigotno otporna na tribenuron metil, kao i jedan neotporan hibrid su gajeni na MS podlozi sa pH vrednošću ili 7 ili 8 i sa različitim koncentracijama herbicida Express 50X, čija je aktivna materija tribenuron-metil (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM i 4.0 µM). Ispitivan je uticaj pH i koncentracije herbicida u podlozi na masu nadzemnog dela i korena klijanaca testiranih hibrida. Vizuelne razlike između otpornih i neotpornog hibrida su uočene nakon 5 dana kulture, dok su se razlike između homozigotnih i heterozigotnih otpornih hibrida pojavile nakon 12 dana kulture. Sve testirane koncentracije herbicida i testirani morfološki parametri su se pokazali kao dobri za identifikaciju neotpornih genotipova. Najbolja kombinacija pH i koncentracije herbicida za razlikovanje homozigotnih od heterozigotnih otpornih genotipova je bila 3.0 µM na pH 7, a morfološki parametar masa korena.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "A rapid test for detection of tribenuron-methyl resistance in sunflower, Brzi test za ispitivanje otpornosti suncokreta na tribenuron metil",
pages = "8-1",
number = "1",
volume = "53",
doi = "10.5937/ratpov53-8412"
}
Dimitrijević, A., Imerovski, I., Miladinović, D., Jocić, S., Cvejić, S., Malidža, G., Kovačević, B.,& Šurlan-Momirović, G.. (2016). A rapid test for detection of tribenuron-methyl resistance in sunflower. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 53(1), 1-8.
https://doi.org/10.5937/ratpov53-8412
Dimitrijević A, Imerovski I, Miladinović D, Jocić S, Cvejić S, Malidža G, Kovačević B, Šurlan-Momirović G. A rapid test for detection of tribenuron-methyl resistance in sunflower. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2016;53(1):1-8.
doi:10.5937/ratpov53-8412 .
Dimitrijević, Aleksandra, Imerovski, Ivana, Miladinović, Dragana, Jocić, Siniša, Cvejić, Sandra, Malidža, Goran, Kovačević, Branislav, Šurlan-Momirović, Gordana, "A rapid test for detection of tribenuron-methyl resistance in sunflower" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 53, no. 1 (2016):1-8,
https://doi.org/10.5937/ratpov53-8412 . .

DSpace software copyright © 2002-2015  DuraSpace
About FiVeR | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutions/communitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About FiVeR | Send Feedback

OpenAIRERCUB