A rapid test for detection of tribenuron-methyl resistance in sunflower
Brzi test za ispitivanje otpornosti suncokreta na tribenuron metil

2016
Authors
Dimitrijević, Aleksandra
Imerovski, Ivana

Miladinović, Dragana

Jocić, Siniša

Cvejić, Sandra

Malidža, Goran

Kovačević, Branislav
Šurlan-Momirović, Gordana

Article (Published version)
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Show full item recordAbstract
A rapid laboratory test for detection of tribenuron-methyl resistant sunflower genotypes was developed. Four homozygous and four heterozygous tribenuron-methyl resistant hybrids, as well as a susceptible one were grown on MS medium supplemented with different concentrations of herbicide (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM and 4.0 µM) and with pH either 7 or 8. The effect of medium pH and herbicide concentration on above-ground part and root mass of sunflower seedlings was observed. The test enabled visual discrimination between resistant and susceptible genotypes in 5 days, and discrimination between homozygous and heterozygous resistant genotypes in 12 days. All tested herbicide concentrations and morphological parameters were found to be suitable for discrimination of tribenuron-methyl susceptible genotypes. The best pH and herbicide concentration combination and morphological parameter for discrimination between homozygous and heterozygous resistant sunflower genotypes were 3.0 µM at pH... 7 and root mass, respectively.
Razvijen je brzi laboratorijski test za detekciju genotipova suncokreta otpornih na tribenuron metil. Po četiri hibrida suncokreta homozigotno i heterozigotno otporna na tribenuron metil, kao i jedan neotporan hibrid su gajeni na MS podlozi sa pH vrednošću ili 7 ili 8 i sa različitim koncentracijama herbicida Express 50X, čija je aktivna materija tribenuron-metil (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM i 4.0 µM). Ispitivan je uticaj pH i koncentracije herbicida u podlozi na masu nadzemnog dela i korena klijanaca testiranih hibrida. Vizuelne razlike između otpornih i neotpornog hibrida su uočene nakon 5 dana kulture, dok su se razlike između homozigotnih i heterozigotnih otpornih hibrida pojavile nakon 12 dana kulture. Sve testirane koncentracije herbicida i testirani morfološki parametri su se pokazali kao dobri za identifikaciju neotpornih genotipova. Najbolja kombinacija pH i koncentracije herbicida za razlikovanje homozigotnih od heterozigotnih otpornih genotipova je bila 3.0 µM na pH 7, a ...morfološki parametar masa korena.
Keywords:
bioassay / Helianthus annuus L. / herbicides / selection / sulfonylureas / tolerance / biološka proba / Helianthus annuus L. / herbicidi / selekcija / sulfoniluree / tolerantnostSource:
Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 2016, 53, 1, 1-8Publisher:
- Institut za ratarstvo i povrtarstvo, Novi Sad
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FiVeRTY - JOUR AU - Dimitrijević, Aleksandra AU - Imerovski, Ivana AU - Miladinović, Dragana AU - Jocić, Siniša AU - Cvejić, Sandra AU - Malidža, Goran AU - Kovačević, Branislav AU - Šurlan-Momirović, Gordana PY - 2016 UR - http://fiver.ifvcns.rs/handle/123456789/1526 AB - A rapid laboratory test for detection of tribenuron-methyl resistant sunflower genotypes was developed. Four homozygous and four heterozygous tribenuron-methyl resistant hybrids, as well as a susceptible one were grown on MS medium supplemented with different concentrations of herbicide (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM and 4.0 µM) and with pH either 7 or 8. The effect of medium pH and herbicide concentration on above-ground part and root mass of sunflower seedlings was observed. The test enabled visual discrimination between resistant and susceptible genotypes in 5 days, and discrimination between homozygous and heterozygous resistant genotypes in 12 days. All tested herbicide concentrations and morphological parameters were found to be suitable for discrimination of tribenuron-methyl susceptible genotypes. The best pH and herbicide concentration combination and morphological parameter for discrimination between homozygous and heterozygous resistant sunflower genotypes were 3.0 µM at pH 7 and root mass, respectively. AB - Razvijen je brzi laboratorijski test za detekciju genotipova suncokreta otpornih na tribenuron metil. Po četiri hibrida suncokreta homozigotno i heterozigotno otporna na tribenuron metil, kao i jedan neotporan hibrid su gajeni na MS podlozi sa pH vrednošću ili 7 ili 8 i sa različitim koncentracijama herbicida Express 50X, čija je aktivna materija tribenuron-metil (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM i 4.0 µM). Ispitivan je uticaj pH i koncentracije herbicida u podlozi na masu nadzemnog dela i korena klijanaca testiranih hibrida. Vizuelne razlike između otpornih i neotpornog hibrida su uočene nakon 5 dana kulture, dok su se razlike između homozigotnih i heterozigotnih otpornih hibrida pojavile nakon 12 dana kulture. Sve testirane koncentracije herbicida i testirani morfološki parametri su se pokazali kao dobri za identifikaciju neotpornih genotipova. Najbolja kombinacija pH i koncentracije herbicida za razlikovanje homozigotnih od heterozigotnih otpornih genotipova je bila 3.0 µM na pH 7, a morfološki parametar masa korena. PB - Institut za ratarstvo i povrtarstvo, Novi Sad T2 - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research T1 - A rapid test for detection of tribenuron-methyl resistance in sunflower T1 - Brzi test za ispitivanje otpornosti suncokreta na tribenuron metil EP - 8 IS - 1 SP - 1 VL - 53 DO - 10.5937/ratpov53-8412 ER -
@article{ author = "Dimitrijević, Aleksandra and Imerovski, Ivana and Miladinović, Dragana and Jocić, Siniša and Cvejić, Sandra and Malidža, Goran and Kovačević, Branislav and Šurlan-Momirović, Gordana", year = "2016", abstract = "A rapid laboratory test for detection of tribenuron-methyl resistant sunflower genotypes was developed. Four homozygous and four heterozygous tribenuron-methyl resistant hybrids, as well as a susceptible one were grown on MS medium supplemented with different concentrations of herbicide (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM and 4.0 µM) and with pH either 7 or 8. The effect of medium pH and herbicide concentration on above-ground part and root mass of sunflower seedlings was observed. The test enabled visual discrimination between resistant and susceptible genotypes in 5 days, and discrimination between homozygous and heterozygous resistant genotypes in 12 days. All tested herbicide concentrations and morphological parameters were found to be suitable for discrimination of tribenuron-methyl susceptible genotypes. The best pH and herbicide concentration combination and morphological parameter for discrimination between homozygous and heterozygous resistant sunflower genotypes were 3.0 µM at pH 7 and root mass, respectively., Razvijen je brzi laboratorijski test za detekciju genotipova suncokreta otpornih na tribenuron metil. Po četiri hibrida suncokreta homozigotno i heterozigotno otporna na tribenuron metil, kao i jedan neotporan hibrid su gajeni na MS podlozi sa pH vrednošću ili 7 ili 8 i sa različitim koncentracijama herbicida Express 50X, čija je aktivna materija tribenuron-metil (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM i 4.0 µM). Ispitivan je uticaj pH i koncentracije herbicida u podlozi na masu nadzemnog dela i korena klijanaca testiranih hibrida. Vizuelne razlike između otpornih i neotpornog hibrida su uočene nakon 5 dana kulture, dok su se razlike između homozigotnih i heterozigotnih otpornih hibrida pojavile nakon 12 dana kulture. Sve testirane koncentracije herbicida i testirani morfološki parametri su se pokazali kao dobri za identifikaciju neotpornih genotipova. Najbolja kombinacija pH i koncentracije herbicida za razlikovanje homozigotnih od heterozigotnih otpornih genotipova je bila 3.0 µM na pH 7, a morfološki parametar masa korena.", publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad", journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research", title = "A rapid test for detection of tribenuron-methyl resistance in sunflower, Brzi test za ispitivanje otpornosti suncokreta na tribenuron metil", pages = "8-1", number = "1", volume = "53", doi = "10.5937/ratpov53-8412" }
Dimitrijević, A., Imerovski, I., Miladinović, D., Jocić, S., Cvejić, S., Malidža, G., Kovačević, B.,& Šurlan-Momirović, G.. (2016). A rapid test for detection of tribenuron-methyl resistance in sunflower. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research Institut za ratarstvo i povrtarstvo, Novi Sad., 53(1), 1-8. https://doi.org/10.5937/ratpov53-8412
Dimitrijević A, Imerovski I, Miladinović D, Jocić S, Cvejić S, Malidža G, Kovačević B, Šurlan-Momirović G. A rapid test for detection of tribenuron-methyl resistance in sunflower. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2016;53(1):1-8. doi:10.5937/ratpov53-8412 .
Dimitrijević, Aleksandra, Imerovski, Ivana, Miladinović, Dragana, Jocić, Siniša, Cvejić, Sandra, Malidža, Goran, Kovačević, Branislav, Šurlan-Momirović, Gordana, "A rapid test for detection of tribenuron-methyl resistance in sunflower" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 53, no. 1 (2016):1-8, https://doi.org/10.5937/ratpov53-8412 . .