Use of oxalic acid for screening intact sunflower plants for resistance to Sclerotinia in vitro
|Three sunflower inbred lines differing in resistance to Sclerotinia were grown on nutritive medium supplemented with different concentrations of oxalic acid. After two weeks of culture, plant height, fresh and dry weight of above-ground part, length of first pair of leaves, root length, and fresh and dry weight of root were measured. Data obtained were analysed using ANOVA and LSD test. Out of the tested parameters, plant height and root length were found to be the most reliable indicators of plant resistance/susceptibility to Sclerotinia. Based on the data obtained, oxalic acid concentrations of 4 mM, 3 mM and 2 mM were chosen for further work in which test would be done on a larger number of genotypes.
|Institut za ratarstvo i povrtarstvo, Novi Sad
|in vitro screening
|Use of oxalic acid for screening intact sunflower plants for resistance to Sclerotinia in vitro