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dc.creatorTaški-Ajduković, Ksenija
dc.creatorNagl, Nevena
dc.creatorMilić, Dragan
dc.creatorKatić, Slobodan
dc.creatorZorić, Miroslav
dc.date.accessioned2021-04-26T19:13:07Z
dc.date.available2021-04-26T19:13:07Z
dc.date.issued2014
dc.identifier.issn1895-104X
dc.identifier.urihttp://fiver.ifvcns.rs/handle/123456789/1361
dc.description.abstractThe aim of investigation was to evaluate genetic variation and relationship among alfalfa populations and their offspring, with minimal cost, by using DNA marker analysis. RAPD analysis was performed on bulked DNA samples of five alfalfa parental populations and their progenies: 20 F1 populations from reciprocal diallel crosses and five S1 populations from self-pollination. Twenty primers generated 217 bands, ranging in size from 300 to 6000 bp, with the average number of bands per primer of 10.85 and polymorphism information content of 0.246. Percentage of polymorphic loci, effective number of alleles, expected heterozygosity and Shannon's information index were used to estimate genetic variation. Higher diversity was observed in F1 progeny populations, while genetic variation in parental populations and S1 progenies remained similar. The genetic relatedness of alfalfa populations was analysed by UPGMA and Bayesian model-based clustering approach. In both types of analysis selfpollinated progenies were grouped. Furthermore, the hybrid offspring where Zuzana, and RSI 20 were maternal parents were placed in separate groups. The results indicate that use of RAPD markers on bulked DNA samples can be fast and cost-effective way for differentiation of alfalfa parental populations and their offspring, as well as for evaluation of their genetic relationships.en
dc.publisherSciendo, Warsaw
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31024/RS//
dc.rightsrestrictedAccess
dc.sourceCentral European Journal of Biology
dc.subjectMedicago sativaen
dc.subjectBulked DNAen
dc.subjectGenetic diversityen
dc.subjectClusteringen
dc.subjectPlant breedingen
dc.titleGenetic variation and relationship of alfalfa populations and their progenies based on RAPD markersen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage776
dc.citation.issue8
dc.citation.other9(8): 768-776
dc.citation.rankM23
dc.citation.spage768
dc.citation.volume9
dc.identifier.doi10.2478/s11535-014-0307-0
dc.identifier.rcubconv_2376
dc.identifier.scopus2-s2.0-84902662000
dc.identifier.wos000338217100005
dc.type.versionpublishedVersion


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