FiVeR - Repository of the Institute of Field and Vegetable Crops
Institute of Field and Vegetable Crops
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   FiVeR
  • FiVeR
  • Radovi istraživača / Researchers' papers
  • View Item
  •   FiVeR
  • FiVeR
  • Radovi istraživača / Researchers' papers
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Genetic variation and relationship of alfalfa populations and their progenies based on RAPD markers

Authorized Users Only
2014
Authors
Taški-Ajduković, Ksenija
Nagl, Nevena
Milić, Dragan
Katić, Slobodan
Zorić, Miroslav
article (publishedVersion)
Metadata
Show full item record
Abstract
The aim of investigation was to evaluate genetic variation and relationship among alfalfa populations and their offspring, with minimal cost, by using DNA marker analysis. RAPD analysis was performed on bulked DNA samples of five alfalfa parental populations and their progenies: 20 F1 populations from reciprocal diallel crosses and five S1 populations from self-pollination. Twenty primers generated 217 bands, ranging in size from 300 to 6000 bp, with the average number of bands per primer of 10.85 and polymorphism information content of 0.246. Percentage of polymorphic loci, effective number of alleles, expected heterozygosity and Shannon's information index were used to estimate genetic variation. Higher diversity was observed in F1 progeny populations, while genetic variation in parental populations and S1 progenies remained similar. The genetic relatedness of alfalfa populations was analysed by UPGMA and Bayesian model-based clustering approach. In both types of analysis selfpollina...ted progenies were grouped. Furthermore, the hybrid offspring where Zuzana, and RSI 20 were maternal parents were placed in separate groups. The results indicate that use of RAPD markers on bulked DNA samples can be fast and cost-effective way for differentiation of alfalfa parental populations and their offspring, as well as for evaluation of their genetic relationships.

Keywords:
Medicago sativa / Bulked DNA / Genetic diversity / Clustering / Plant breeding
Source:
Central European Journal of Biology, 2014, 9, 8, 768-776
Publisher:
  • Sciendo, Warsaw
Funding / projects:
  • info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31024/RS// (RS-31024)

DOI: 10.2478/s11535-014-0307-0

ISSN: 1895-104X

WoS: 000338217100005

Scopus: 2-s2.0-84902662000
[ Google Scholar ]
URI
http://fiver.ifvcns.rs/handle/123456789/1361
Collections
  • Radovi istraživača / Researchers' papers
Institution/Community
FiVeR

DSpace software copyright © 2002-2015  DuraSpace
About FiVeR | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutions/communitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About FiVeR | Send Feedback

OpenAIRERCUB