Broomrape is a parasitic plant that significantly decreases yield of sunflower. Breeding for resistance has proved to be the most efficient method for suppressing broomrape infestation in the field; however, new races of parasite constantly emerge, and new resistance genes need to be discovered and introduced into cultivated sunflower lines. The aim of this work was to test SSR markers from linkage group 3 (LG3) to investigate whether they could be used for identification of a particular Or gene. Twenty sunflower inbred lines were used, and polymorphism between the lines with various resistance genes and genetic background was investigated. The used markers revealed DNA polymorphism between the investigated lines. Strong association of markers from LG3 with Or6, as well as Or4 and Or2 genes, was found. Identified markers could be used for introduction of these resistance genes into commercial sunflower lines and for establishment and identification of differential lines.
TY - JOUR
AU - Imerovski, Ivana
AU - Dimitrijević, Aleksandra
AU - Miladinović, Dragana
AU - Dedić, Boško
AU - Jocić, Siniša
AU - Kovačević, Branislav
AU - Obreht, Dragana
PY - 2013
UR - http://fiver.ifvcns.rs/handle/123456789/1244
AB - Broomrape is a parasitic plant that significantly decreases yield of sunflower. Breeding for resistance has proved to be the most efficient method for suppressing broomrape infestation in the field; however, new races of parasite constantly emerge, and new resistance genes need to be discovered and introduced into cultivated sunflower lines. The aim of this work was to test SSR markers from linkage group 3 (LG3) to investigate whether they could be used for identification of a particular Or gene. Twenty sunflower inbred lines were used, and polymorphism between the lines with various resistance genes and genetic background was investigated. The used markers revealed DNA polymorphism between the investigated lines. Strong association of markers from LG3 with Or6, as well as Or4 and Or2 genes, was found. Identified markers could be used for introduction of these resistance genes into commercial sunflower lines and for establishment and identification of differential lines.
PB - Wiley, Hoboken
T2 - Plant Breeding
T1 - Identification of PCR markers linked to different Or genes in sunflower
EP - 120
IS - 1
SP - 115
VL - 132
DO - 10.1111/pbr.12022
ER -
@article{
author = "Imerovski, Ivana and Dimitrijević, Aleksandra and Miladinović, Dragana and Dedić, Boško and Jocić, Siniša and Kovačević, Branislav and Obreht, Dragana",
year = "2013",
abstract = "Broomrape is a parasitic plant that significantly decreases yield of sunflower. Breeding for resistance has proved to be the most efficient method for suppressing broomrape infestation in the field; however, new races of parasite constantly emerge, and new resistance genes need to be discovered and introduced into cultivated sunflower lines. The aim of this work was to test SSR markers from linkage group 3 (LG3) to investigate whether they could be used for identification of a particular Or gene. Twenty sunflower inbred lines were used, and polymorphism between the lines with various resistance genes and genetic background was investigated. The used markers revealed DNA polymorphism between the investigated lines. Strong association of markers from LG3 with Or6, as well as Or4 and Or2 genes, was found. Identified markers could be used for introduction of these resistance genes into commercial sunflower lines and for establishment and identification of differential lines.",
publisher = "Wiley, Hoboken",
journal = "Plant Breeding",
title = "Identification of PCR markers linked to different Or genes in sunflower",
pages = "120-115",
number = "1",
volume = "132",
doi = "10.1111/pbr.12022"
}
Imerovski, I., Dimitrijević, A., Miladinović, D., Dedić, B., Jocić, S., Kovačević, B.,& Obreht, D.. (2013). Identification of PCR markers linked to different Or genes in sunflower. in Plant Breeding
Wiley, Hoboken., 132(1), 115-120.
https://doi.org/10.1111/pbr.12022
Imerovski I, Dimitrijević A, Miladinović D, Dedić B, Jocić S, Kovačević B, Obreht D. Identification of PCR markers linked to different Or genes in sunflower. in Plant Breeding. 2013;132(1):115-120.
doi:10.1111/pbr.12022 .
Imerovski, Ivana, Dimitrijević, Aleksandra, Miladinović, Dragana, Dedić, Boško, Jocić, Siniša, Kovačević, Branislav, Obreht, Dragana, "Identification of PCR markers linked to different Or genes in sunflower" in Plant Breeding, 132, no. 1 (2013):115-120,
https://doi.org/10.1111/pbr.12022 . .
