Colletotrichum acutatum and Colletotrichum gloeosporioides are important post harvest apple fruit pathogens in temperate regions. Seven Colletotrichum isolates obtained from apple fruits with anthracnose symptoms, and two reference isolates C. gloeosporioides AVO 37 4B (avocado, Israel) and C. actuatum TUT 137 A (strawberry, Israel) were investigated. Pathogenicity of all nine isolates was demonstrated on wound-inoculated apple fruits by fulfilling Koch's postulates. Investigated morphological characteristics involved shape, colour and margin of the colony, as well as conidial shape. Observed ecological parameters were growth and sporulation of isolates after 4, 7 and 11 days of incubation at temperatures of 15, 20, 23, 25, 27, 30, 32 and 35 degrees C and after 10 days of incubation under conditions of day light regime and dark. The isolates were identified by PCR using species-specific primers. The data regarding growth were processed by factorial and one way ANOVA using software Stat...istica 10. All seven isolates obtained from apple fruits and reference C. acutatum isolate formed velvety, gray colonies with slightly wavy margin, and one celled fusiform conidia on PDA medium. Mycelium of the reference isolate C. gloeosporioides AVO 37 4B was snow-white and conidia were cylindrical. Maximum mycelial growth rate for isolates obtained from apple fruits was at temperature of 23 degrees C, for reference isolate C. acutatum at 27 degrees C and for C. gloeosporioides at 23-27 degrees C. Temperature of 35 degrees C proved to be restrictive for C. acutatum isolates. Isolate C. gloeosporioides had the fastest growth rate compared to all other isolates. Using PCR with species-specific primers all eight isolates were identified as C. acutatum (fragments sized 490 bp were amplified), and one as C. gloeosporioides (fragments sized 450 bp were amplified). The results suggest that differentiation between the two Colletotrichum species based on several morphological and ecological parameters is possible.
Keywords:
apple / anthracnose / identification / Colletotrichum spp.
Source:
Zemdirbyste, 2012, 99, 2, 189-196
Publisher:
Lithuanian Research Centre for Agriculture and Forestry; Vytautas Magnus University
Funding / projects:
Ministry of Education and Science of the Republic of Serbia
TY - JOUR
AU - Grahovac, Mila
AU - Inđić, Dušanka
AU - Vuković, Slavica
AU - Hrustić, Jovana
AU - Gvozdenac, Sonja
AU - Mihajlović, Milica
AU - Tanović, Brankica
PY - 2012
UR - http://fiver.ifvcns.rs/handle/123456789/1173
AB - Colletotrichum acutatum and Colletotrichum gloeosporioides are important post harvest apple fruit pathogens in temperate regions. Seven Colletotrichum isolates obtained from apple fruits with anthracnose symptoms, and two reference isolates C. gloeosporioides AVO 37 4B (avocado, Israel) and C. actuatum TUT 137 A (strawberry, Israel) were investigated. Pathogenicity of all nine isolates was demonstrated on wound-inoculated apple fruits by fulfilling Koch's postulates. Investigated morphological characteristics involved shape, colour and margin of the colony, as well as conidial shape. Observed ecological parameters were growth and sporulation of isolates after 4, 7 and 11 days of incubation at temperatures of 15, 20, 23, 25, 27, 30, 32 and 35 degrees C and after 10 days of incubation under conditions of day light regime and dark. The isolates were identified by PCR using species-specific primers. The data regarding growth were processed by factorial and one way ANOVA using software Statistica 10. All seven isolates obtained from apple fruits and reference C. acutatum isolate formed velvety, gray colonies with slightly wavy margin, and one celled fusiform conidia on PDA medium. Mycelium of the reference isolate C. gloeosporioides AVO 37 4B was snow-white and conidia were cylindrical. Maximum mycelial growth rate for isolates obtained from apple fruits was at temperature of 23 degrees C, for reference isolate C. acutatum at 27 degrees C and for C. gloeosporioides at 23-27 degrees C. Temperature of 35 degrees C proved to be restrictive for C. acutatum isolates. Isolate C. gloeosporioides had the fastest growth rate compared to all other isolates. Using PCR with species-specific primers all eight isolates were identified as C. acutatum (fragments sized 490 bp were amplified), and one as C. gloeosporioides (fragments sized 450 bp were amplified). The results suggest that differentiation between the two Colletotrichum species based on several morphological and ecological parameters is possible.
PB - Lithuanian Research Centre for Agriculture and Forestry; Vytautas Magnus University
T2 - Zemdirbyste
T1 - Morphological and ecological features as differentiation criteria for Colletotrichum species
EP - 196
IS - 2
SP - 189
VL - 99
UR - https://hdl.handle.net/21.15107/rcub_fiver_1173
ER -
@article{
author = "Grahovac, Mila and Inđić, Dušanka and Vuković, Slavica and Hrustić, Jovana and Gvozdenac, Sonja and Mihajlović, Milica and Tanović, Brankica",
year = "2012",
abstract = "Colletotrichum acutatum and Colletotrichum gloeosporioides are important post harvest apple fruit pathogens in temperate regions. Seven Colletotrichum isolates obtained from apple fruits with anthracnose symptoms, and two reference isolates C. gloeosporioides AVO 37 4B (avocado, Israel) and C. actuatum TUT 137 A (strawberry, Israel) were investigated. Pathogenicity of all nine isolates was demonstrated on wound-inoculated apple fruits by fulfilling Koch's postulates. Investigated morphological characteristics involved shape, colour and margin of the colony, as well as conidial shape. Observed ecological parameters were growth and sporulation of isolates after 4, 7 and 11 days of incubation at temperatures of 15, 20, 23, 25, 27, 30, 32 and 35 degrees C and after 10 days of incubation under conditions of day light regime and dark. The isolates were identified by PCR using species-specific primers. The data regarding growth were processed by factorial and one way ANOVA using software Statistica 10. All seven isolates obtained from apple fruits and reference C. acutatum isolate formed velvety, gray colonies with slightly wavy margin, and one celled fusiform conidia on PDA medium. Mycelium of the reference isolate C. gloeosporioides AVO 37 4B was snow-white and conidia were cylindrical. Maximum mycelial growth rate for isolates obtained from apple fruits was at temperature of 23 degrees C, for reference isolate C. acutatum at 27 degrees C and for C. gloeosporioides at 23-27 degrees C. Temperature of 35 degrees C proved to be restrictive for C. acutatum isolates. Isolate C. gloeosporioides had the fastest growth rate compared to all other isolates. Using PCR with species-specific primers all eight isolates were identified as C. acutatum (fragments sized 490 bp were amplified), and one as C. gloeosporioides (fragments sized 450 bp were amplified). The results suggest that differentiation between the two Colletotrichum species based on several morphological and ecological parameters is possible.",
publisher = "Lithuanian Research Centre for Agriculture and Forestry; Vytautas Magnus University",
journal = "Zemdirbyste",
title = "Morphological and ecological features as differentiation criteria for Colletotrichum species",
pages = "196-189",
number = "2",
volume = "99",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1173"
}
Grahovac, M., Inđić, D., Vuković, S., Hrustić, J., Gvozdenac, S., Mihajlović, M.,& Tanović, B.. (2012). Morphological and ecological features as differentiation criteria for Colletotrichum species. in Zemdirbyste
Lithuanian Research Centre for Agriculture and Forestry; Vytautas Magnus University., 99(2), 189-196.
https://hdl.handle.net/21.15107/rcub_fiver_1173
Grahovac M, Inđić D, Vuković S, Hrustić J, Gvozdenac S, Mihajlović M, Tanović B. Morphological and ecological features as differentiation criteria for Colletotrichum species. in Zemdirbyste. 2012;99(2):189-196.
https://hdl.handle.net/21.15107/rcub_fiver_1173 .
Grahovac, Mila, Inđić, Dušanka, Vuković, Slavica, Hrustić, Jovana, Gvozdenac, Sonja, Mihajlović, Milica, Tanović, Brankica, "Morphological and ecological features as differentiation criteria for Colletotrichum species" in Zemdirbyste, 99, no. 2 (2012):189-196,
https://hdl.handle.net/21.15107/rcub_fiver_1173 .
