TY  - JOUR
AU  - Horn, Renate
AU  - Radanović, Aleksandra
AU  - Fuhrmann, Lena
AU  - Sprycha, Yves
AU  - Hamrit, Sonia
AU  - Jocković, Milan
AU  - Miladinović, Dragana
AU  - Jansen, Constantin
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1927
AB  - Hybrid breeding in sunflowers based on CMS PET1 requires development of restorer lines carrying, in most cases, the restorer gene Rf1. Markers for marker-assisted selection have been developed, but there is still need for closer, more versatile, and co-dominant markers linked to Rf1. Homology searches against the reference sunflower genome using sequences of cloned markers, as well as Bacterial Artificial Chromosome (BAC)-end sequences of clones hybridizing to them, allowed the identification of two genomic regions of 30 and 3.9 Mb, respectively, as possible physical locations of the restorer gene Rf1 on linkage group 13. Nine potential candidate genes, encoding six pentatricopeptide repeat proteins, one tetratricopeptide-like helical domain, a probable aldehyde dehydrogenase 22A1, and a probable poly(A) polymerase 3 (PAPS3), were identified in these two genomic regions. Amplicon targeted next generation sequencing of these nine candidate genes for Rf1 was performed in an association panel consisting of 27 maintainer and 32 restorer lines and revealed the presence of 210 Single Nucleotide Polymorphisms (SNPs) and 67 Insertions/Deletions (INDELs). Association studies showed significant associations of 10 SNPs with fertility restoration (p-value  lt  10(-4)), narrowing Rf1 down to three candidate genes. Three new markers, one co-dominant marker 67N04_P and two dominant markers, PPR621.5R for restorer, and PPR621.5M for maintainer lines were developed and verified in the association panel of 59 sunflower lines. The versatility of the three newly developed markers, as well as of three existing markers for the restorer gene Rf1 (HRG01 and HRG02, Cleaved Amplified Polymorphic Sequence (CAPS)-marker H13), was analyzed in a large association panel consisting of 557 accessions.
PB  - Basel : MDPI
T2  - International Journal of Molecular Sciences
T1  - Development and Validation of Markers for the Fertility Restorer Gene Rf1 in Sunflower
IS  - 6
VL  - 20
DO  - 10.3390/ijms20061260
ER  - 

@article{
author = "Horn, Renate and Radanović, Aleksandra and Fuhrmann, Lena and Sprycha, Yves and Hamrit, Sonia and Jocković, Milan and Miladinović, Dragana and Jansen, Constantin",
year = "2019",
abstract = "Hybrid breeding in sunflowers based on CMS PET1 requires development of restorer lines carrying, in most cases, the restorer gene Rf1. Markers for marker-assisted selection have been developed, but there is still need for closer, more versatile, and co-dominant markers linked to Rf1. Homology searches against the reference sunflower genome using sequences of cloned markers, as well as Bacterial Artificial Chromosome (BAC)-end sequences of clones hybridizing to them, allowed the identification of two genomic regions of 30 and 3.9 Mb, respectively, as possible physical locations of the restorer gene Rf1 on linkage group 13. Nine potential candidate genes, encoding six pentatricopeptide repeat proteins, one tetratricopeptide-like helical domain, a probable aldehyde dehydrogenase 22A1, and a probable poly(A) polymerase 3 (PAPS3), were identified in these two genomic regions. Amplicon targeted next generation sequencing of these nine candidate genes for Rf1 was performed in an association panel consisting of 27 maintainer and 32 restorer lines and revealed the presence of 210 Single Nucleotide Polymorphisms (SNPs) and 67 Insertions/Deletions (INDELs). Association studies showed significant associations of 10 SNPs with fertility restoration (p-value  lt  10(-4)), narrowing Rf1 down to three candidate genes. Three new markers, one co-dominant marker 67N04_P and two dominant markers, PPR621.5R for restorer, and PPR621.5M for maintainer lines were developed and verified in the association panel of 59 sunflower lines. The versatility of the three newly developed markers, as well as of three existing markers for the restorer gene Rf1 (HRG01 and HRG02, Cleaved Amplified Polymorphic Sequence (CAPS)-marker H13), was analyzed in a large association panel consisting of 557 accessions.",
publisher = "Basel : MDPI",
journal = "International Journal of Molecular Sciences",
title = "Development and Validation of Markers for the Fertility Restorer Gene Rf1 in Sunflower",
number = "6",
volume = "20",
doi = "10.3390/ijms20061260"
}

Horn, R., Radanović, A., Fuhrmann, L., Sprycha, Y., Hamrit, S., Jocković, M., Miladinović, D.,& Jansen, C.. (2019). Development and Validation of Markers for the Fertility Restorer Gene Rf1 in Sunflower. in International Journal of Molecular Sciences
Basel : MDPI., 20(6).
https://doi.org/10.3390/ijms20061260

Horn R, Radanović A, Fuhrmann L, Sprycha Y, Hamrit S, Jocković M, Miladinović D, Jansen C. Development and Validation of Markers for the Fertility Restorer Gene Rf1 in Sunflower. in International Journal of Molecular Sciences. 2019;20(6).
doi:10.3390/ijms20061260 .

Horn, Renate, Radanović, Aleksandra, Fuhrmann, Lena, Sprycha, Yves, Hamrit, Sonia, Jocković, Milan, Miladinović, Dragana, Jansen, Constantin, "Development and Validation of Markers for the Fertility Restorer Gene Rf1 in Sunflower" in International Journal of Molecular Sciences, 20, no. 6 (2019),
https://doi.org/10.3390/ijms20061260 . .