TY  - JOUR
AU  - Miljaković, Dragana
AU  - Marinković, Jelena
AU  - Tamindžić, Gordana
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Karačić, Vasiljka
AU  - Jakšić, Snežana
PY  - 2024
UR  - http://fiver.ifvcns.rs/handle/123456789/4641
AB  - Seed infection caused by Fusarium spp. is one of the major threats to the seed quality and
yield of agricultural crops, including garden peas. The use of Bacillus spp. with multiple antagonistic
and plant growth-promoting (PGP) abilities represents a potential disease control strategy. This
study was performed to evaluate the biocontrol potential of new Bacillus spp. rhizosphere isolates
against two Fusarium strains affecting garden peas. Six Bacillus isolates identified by 16S rDNA sequencing as B. velezensis (B42), B. subtilis (B43), B. mojavensis (B44, B46), B. amyloliquefaciens (B50),
and B. halotolerans (B66) showed the highest in vitro inhibition of F. proliferatum PS1 and F. equiseti
PS18 growth (over 40%). The selected Bacillus isolates possessed biosynthetic genes for endoglucanase (B42, B43, B50), surfactin (B43, B44, B46), fengycin (B44, B46), bacillomycin D (B42, B50), and
iturin (B42), and were able to produce indole-3-acetic acid (IAA), siderophores, and cellulase. Two
isolates, B. subtilis B43 and B. amyloliquefaciens B50, had the highest effect on final germination, shoot
length, root length, shoot dry weight, root dry weight, and seedling vigor index of garden peas as
compared to the control. Their individual or combined application reduced seed infection and increased seed germination in the presence of F. proliferatum PS1 and F. equiseti PS18, both after seed
inoculation and seed bio-priming. The most promising results were obtained in the cases of the
bacterial consortium, seed bio-priming, and the more pathogenic strain PS18. The novel Bacillus
isolates may be potential biocontrol agents intended for the management of Fusarium seed-borne
diseases.
PB  - Basel : MDPI
T2  - Journal of Fungi
T1  - Bio-Priming with Bacillus Isolates Suppresses Seed Infection and Improves the Germination of Garden Peas in the Presence of Fusarium Strains
IS  - 5
SP  - 358
VL  - 10
DO  - 10.3390/jof10050358
ER  - 

@article{
author = "Miljaković, Dragana and Marinković, Jelena and Tamindžić, Gordana and Milošević, Dragana and Ignjatov, Maja and Karačić, Vasiljka and Jakšić, Snežana",
year = "2024",
abstract = "Seed infection caused by Fusarium spp. is one of the major threats to the seed quality and
yield of agricultural crops, including garden peas. The use of Bacillus spp. with multiple antagonistic
and plant growth-promoting (PGP) abilities represents a potential disease control strategy. This
study was performed to evaluate the biocontrol potential of new Bacillus spp. rhizosphere isolates
against two Fusarium strains affecting garden peas. Six Bacillus isolates identified by 16S rDNA sequencing as B. velezensis (B42), B. subtilis (B43), B. mojavensis (B44, B46), B. amyloliquefaciens (B50),
and B. halotolerans (B66) showed the highest in vitro inhibition of F. proliferatum PS1 and F. equiseti
PS18 growth (over 40%). The selected Bacillus isolates possessed biosynthetic genes for endoglucanase (B42, B43, B50), surfactin (B43, B44, B46), fengycin (B44, B46), bacillomycin D (B42, B50), and
iturin (B42), and were able to produce indole-3-acetic acid (IAA), siderophores, and cellulase. Two
isolates, B. subtilis B43 and B. amyloliquefaciens B50, had the highest effect on final germination, shoot
length, root length, shoot dry weight, root dry weight, and seedling vigor index of garden peas as
compared to the control. Their individual or combined application reduced seed infection and increased seed germination in the presence of F. proliferatum PS1 and F. equiseti PS18, both after seed
inoculation and seed bio-priming. The most promising results were obtained in the cases of the
bacterial consortium, seed bio-priming, and the more pathogenic strain PS18. The novel Bacillus
isolates may be potential biocontrol agents intended for the management of Fusarium seed-borne
diseases.",
publisher = "Basel : MDPI",
journal = "Journal of Fungi",
title = "Bio-Priming with Bacillus Isolates Suppresses Seed Infection and Improves the Germination of Garden Peas in the Presence of Fusarium Strains",
number = "5",
pages = "358",
volume = "10",
doi = "10.3390/jof10050358"
}

Miljaković, D., Marinković, J., Tamindžić, G., Milošević, D., Ignjatov, M., Karačić, V.,& Jakšić, S.. (2024). Bio-Priming with Bacillus Isolates Suppresses Seed Infection and Improves the Germination of Garden Peas in the Presence of Fusarium Strains. in Journal of Fungi
Basel : MDPI., 10(5), 358.
https://doi.org/10.3390/jof10050358

Miljaković D, Marinković J, Tamindžić G, Milošević D, Ignjatov M, Karačić V, Jakšić S. Bio-Priming with Bacillus Isolates Suppresses Seed Infection and Improves the Germination of Garden Peas in the Presence of Fusarium Strains. in Journal of Fungi. 2024;10(5):358.
doi:10.3390/jof10050358 .

Miljaković, Dragana, Marinković, Jelena, Tamindžić, Gordana, Milošević, Dragana, Ignjatov, Maja, Karačić, Vasiljka, Jakšić, Snežana, "Bio-Priming with Bacillus Isolates Suppresses Seed Infection and Improves the Germination of Garden Peas in the Presence of Fusarium Strains" in Journal of Fungi, 10, no. 5 (2024):358,
https://doi.org/10.3390/jof10050358 . .