TY  - JOUR
AU  - Smykal, Petr
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Zlatković, Bojan
AU  - Ćupina, Branko
AU  - Đorđević, Vuk
AU  - Mikić, Aleksandar
AU  - Medović, Aleksandar
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1380
AB  - The development of agriculture was a key turning point in human history, a central part of which was the evolution of new plant forms, domesticated crops. Grain legumes were domesticated in parallel with cereals and formed important dietary components of early civilizations. First domesticated in the Near East, pea has been cultivated in Europe since the Stone and Bronze Ages. In this study, we present a molecular analysis of ancient DNA (aDNA) extracted from carbonized pea seeds recovered from deposits at Hissar, in southeast Serbia, that date to the eleventh century B.C. Four selected chloroplast DNA loci (trnSG, trnK, matK and rbcL) amplified in six fragments of 128-340 bp with a total length of 1,329 bp were successfully recovered in order to distinguish between cultivated and wild gathered pea. Based on identified mutations, the results showed that genuine aDNA was analyzed. Moreover, DNA analysis resulted in placing the ancient sample at an intermediate position between extant cultivated [Pisum sativum L. and wild P. sativum subsp. elatius (Steven ex M. Bieb.) Asch. et Graebn.]. Consequently, based on a combination of morphological and molecular data, we concluded that the material represents an early domesticated pea. We speculate that Iron Age pea would be of colored flower and pigmented testa, similar to today's fodder pea (P. sativum subsp. sativum var. arvense (L.) Poir.), possibly of winter type. This is the first report of successful aDNA extraction and analysis from any legume species thus far. The implications for pea domestication are discussed here.
PB  - Springer, Dordrecht
T2  - Genetic Resources & Crop Evolution
T1  - A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication
EP  - 1544
IS  - 8
SP  - 1533
VL  - 61
DO  - 10.1007/s10722-014-0128-z
ER  - 

@article{
author = "Smykal, Petr and Jovanović, Živko and Stanisavljević, Nemanja and Zlatković, Bojan and Ćupina, Branko and Đorđević, Vuk and Mikić, Aleksandar and Medović, Aleksandar",
year = "2014",
abstract = "The development of agriculture was a key turning point in human history, a central part of which was the evolution of new plant forms, domesticated crops. Grain legumes were domesticated in parallel with cereals and formed important dietary components of early civilizations. First domesticated in the Near East, pea has been cultivated in Europe since the Stone and Bronze Ages. In this study, we present a molecular analysis of ancient DNA (aDNA) extracted from carbonized pea seeds recovered from deposits at Hissar, in southeast Serbia, that date to the eleventh century B.C. Four selected chloroplast DNA loci (trnSG, trnK, matK and rbcL) amplified in six fragments of 128-340 bp with a total length of 1,329 bp were successfully recovered in order to distinguish between cultivated and wild gathered pea. Based on identified mutations, the results showed that genuine aDNA was analyzed. Moreover, DNA analysis resulted in placing the ancient sample at an intermediate position between extant cultivated [Pisum sativum L. and wild P. sativum subsp. elatius (Steven ex M. Bieb.) Asch. et Graebn.]. Consequently, based on a combination of morphological and molecular data, we concluded that the material represents an early domesticated pea. We speculate that Iron Age pea would be of colored flower and pigmented testa, similar to today's fodder pea (P. sativum subsp. sativum var. arvense (L.) Poir.), possibly of winter type. This is the first report of successful aDNA extraction and analysis from any legume species thus far. The implications for pea domestication are discussed here.",
publisher = "Springer, Dordrecht",
journal = "Genetic Resources & Crop Evolution",
title = "A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication",
pages = "1544-1533",
number = "8",
volume = "61",
doi = "10.1007/s10722-014-0128-z"
}

Smykal, P., Jovanović, Ž., Stanisavljević, N., Zlatković, B., Ćupina, B., Đorđević, V., Mikić, A.,& Medović, A.. (2014). A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication. in Genetic Resources & Crop Evolution
Springer, Dordrecht., 61(8), 1533-1544.
https://doi.org/10.1007/s10722-014-0128-z

Smykal P, Jovanović Ž, Stanisavljević N, Zlatković B, Ćupina B, Đorđević V, Mikić A, Medović A. A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication. in Genetic Resources & Crop Evolution. 2014;61(8):1533-1544.
doi:10.1007/s10722-014-0128-z .

Smykal, Petr, Jovanović, Živko, Stanisavljević, Nemanja, Zlatković, Bojan, Ćupina, Branko, Đorđević, Vuk, Mikić, Aleksandar, Medović, Aleksandar, "A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication" in Genetic Resources & Crop Evolution, 61, no. 8 (2014):1533-1544,
https://doi.org/10.1007/s10722-014-0128-z . .

TY  - CONF
AU  - Mikić, Sanja
AU  - Nastasić, Aleksandra
AU  - Mikić, Aleksandar
AU  - Milisavljević, Mira
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3037
AB  - Intercropping maize (Zea mays L.) with annual legumes is distributed throughout the world, mostly in subtopic and tropic regions of Africa, Asia and South America, including warm season legumes cowpea (Vigna unguiculata (L.) Walp.), pigeonpea (Cajanus cajan (L.) Huth), common bean (Phaseulus vulgaris L.) and soybean (Glycine max (L.) Merr.). Numerous studies of maize/legumes intercropping underline its role in soil erosion control, weed and pests management, water use efficiency, light distribution and use, nutrient availability and uptake, and more efficient use of land (LER>1). Exudates from maize stimulate nodulation biomass production of legumes, whereas maize uptake N stimulates nitrogen fixation in conditions of competition for N. In temperate regions, cool season legumes can be intercropped with maize, where different sowing dates and growth dynamic allow different temporal and spatial use of environmental resources. Such intercrops are grown on smaller areas in developing countries, while in China it is practiced on larger scale, especially with pea (Pisum sativum L.). In Serbia and other Balkan countries, intercropping maize with annual legumes is present in many regions, especially on small fields and in gardens.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Some aspects of amaizing annual legumes
EP  - 261
SP  - 261
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3037
ER  - 

@conference{
author = "Mikić, Sanja and Nastasić, Aleksandra and Mikić, Aleksandar and Milisavljević, Mira",
year = "2013",
abstract = "Intercropping maize (Zea mays L.) with annual legumes is distributed throughout the world, mostly in subtopic and tropic regions of Africa, Asia and South America, including warm season legumes cowpea (Vigna unguiculata (L.) Walp.), pigeonpea (Cajanus cajan (L.) Huth), common bean (Phaseulus vulgaris L.) and soybean (Glycine max (L.) Merr.). Numerous studies of maize/legumes intercropping underline its role in soil erosion control, weed and pests management, water use efficiency, light distribution and use, nutrient availability and uptake, and more efficient use of land (LER>1). Exudates from maize stimulate nodulation biomass production of legumes, whereas maize uptake N stimulates nitrogen fixation in conditions of competition for N. In temperate regions, cool season legumes can be intercropped with maize, where different sowing dates and growth dynamic allow different temporal and spatial use of environmental resources. Such intercrops are grown on smaller areas in developing countries, while in China it is practiced on larger scale, especially with pea (Pisum sativum L.). In Serbia and other Balkan countries, intercropping maize with annual legumes is present in many regions, especially on small fields and in gardens.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Some aspects of amaizing annual legumes",
pages = "261-261",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3037"
}

Mikić, S., Nastasić, A., Mikić, A.,& Milisavljević, M.. (2013). Some aspects of amaizing annual legumes. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 261-261.
https://hdl.handle.net/21.15107/rcub_fiver_3037

Mikić S, Nastasić A, Mikić A, Milisavljević M. Some aspects of amaizing annual legumes. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:261-261.
https://hdl.handle.net/21.15107/rcub_fiver_3037 .

Mikić, Sanja, Nastasić, Aleksandra, Mikić, Aleksandar, Milisavljević, Mira, "Some aspects of amaizing annual legumes" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):261-261,
https://hdl.handle.net/21.15107/rcub_fiver_3037 .

TY  - CONF
AU  - Aleksić, Jelena
AU  - Banović, Bojana
AU  - Mikić, Aleksandar
AU  - Ćupina, Branko
AU  - Zlatković, Bojan
AU  - Anđelković, Snežana
AU  - Maksimović, Vesna
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/2862
AB  - An increasing interest in cultivation and improvement of faba bean (Vicia faba L.), an important food and feed grain legume whose ex siz genetic resources comprise cultivated forms only, triggers both search for novel genotypes and their molecular characterization. Due to the poor representation of faba bean genotypes from the central Balkans in worldwide ex situ collections, we collected seeds of 13 faba bean landraces from this region, preserved them at the French National Institute for Agricultural Research (INRA) ex situ collection and used them also for amplification of ten nuclear microsatellites (simple sequence repeats — SSRs). Given the self-pollinating nature of faba bean and low heterozygosity levels in this crop, we assumed that relatively low number of alleles may be expected in each landrace. Therefore, we first demonstrated that all alleles obtained upon amplification of ten SSRs in 11 individuals from one landrace could be detected when DNAs from these individuals were pooled and such a bulk DNA was used as a template for PCR reactions, and then tested all SSRs in bulk DNA templates from each out of ten remaining landraces comprising six to 15 genotypes.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Preservation and molecular characterization of 13 central Balkan landraces of faba bean (Vicia faba L.)
EP  - 19
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2862
ER  - 

@conference{
author = "Aleksić, Jelena and Banović, Bojana and Mikić, Aleksandar and Ćupina, Branko and Zlatković, Bojan and Anđelković, Snežana and Maksimović, Vesna",
year = "2013",
abstract = "An increasing interest in cultivation and improvement of faba bean (Vicia faba L.), an important food and feed grain legume whose ex siz genetic resources comprise cultivated forms only, triggers both search for novel genotypes and their molecular characterization. Due to the poor representation of faba bean genotypes from the central Balkans in worldwide ex situ collections, we collected seeds of 13 faba bean landraces from this region, preserved them at the French National Institute for Agricultural Research (INRA) ex situ collection and used them also for amplification of ten nuclear microsatellites (simple sequence repeats — SSRs). Given the self-pollinating nature of faba bean and low heterozygosity levels in this crop, we assumed that relatively low number of alleles may be expected in each landrace. Therefore, we first demonstrated that all alleles obtained upon amplification of ten SSRs in 11 individuals from one landrace could be detected when DNAs from these individuals were pooled and such a bulk DNA was used as a template for PCR reactions, and then tested all SSRs in bulk DNA templates from each out of ten remaining landraces comprising six to 15 genotypes.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Preservation and molecular characterization of 13 central Balkan landraces of faba bean (Vicia faba L.)",
pages = "19-19",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2862"
}

Aleksić, J., Banović, B., Mikić, A., Ćupina, B., Zlatković, B., Anđelković, S.,& Maksimović, V.. (2013). Preservation and molecular characterization of 13 central Balkan landraces of faba bean (Vicia faba L.). in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 19-19.
https://hdl.handle.net/21.15107/rcub_fiver_2862

Aleksić J, Banović B, Mikić A, Ćupina B, Zlatković B, Anđelković S, Maksimović V. Preservation and molecular characterization of 13 central Balkan landraces of faba bean (Vicia faba L.). in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:19-19.
https://hdl.handle.net/21.15107/rcub_fiver_2862 .

Aleksić, Jelena, Banović, Bojana, Mikić, Aleksandar, Ćupina, Branko, Zlatković, Bojan, Anđelković, Snežana, Maksimović, Vesna, "Preservation and molecular characterization of 13 central Balkan landraces of faba bean (Vicia faba L.)" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):19-19,
https://hdl.handle.net/21.15107/rcub_fiver_2862 .

TY  - CONF
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Fira, Đorđe
AU  - Jovanović, Živko
AU  - Miljuš-Đukić, Jovanka
AU  - Radović, Svetlana
AU  - Mikić, Aleksandar
AU  - Maksimović, Vesna
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3040
AB  - In recent years antioxidant properties of plant derived protein hydrolysates were established. Antioxidative activity of these hydrolysates largely depends on specificity of the proteinase used for hydrolysis, duration of proteinase treatment, as well as composition and molecular weight of hydrolysis derived peptides. In order to explore potential use of nine proteinase containing Lactobacillus strains in production of antioxidative peptides we tested their ability to hydrolyze lyophilized hydrosoluble protein fraction from dehulled and defatted dry pea seeds (Pisum sativum), cultivar NS Mraz. We also developed plant protein based liquid medium for cultivation of lactobacilli which was later used as starter for hydrolysis reaction in larger volume. We found that all nine strains could hydrolyze plant hydrosoluble fraction of pea seed proteins but with different efficiency. We also examined the effect of duration of hydrolysis on antioxidative activity in <10kDa fraction of obtained peptide extracts, using most proteolyticaly active strain, human vaginal isolate Lactobacillus rhamnosus T10. General conclusion was that antioxidative activity was positively correlated with increase of hydrolysis duration and amount of small peptides, which reached up to 34% of total proteins after 24h of hydrolysis treatment.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Application of different Lactobacillus strains in production pea seed protein hydrolysates with antioxidative activity
EP  - 286
SP  - 286
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3040
ER  - 

@conference{
author = "Stanisavljević, Nemanja and Vukotić, Goran and Fira, Đorđe and Jovanović, Živko and Miljuš-Đukić, Jovanka and Radović, Svetlana and Mikić, Aleksandar and Maksimović, Vesna",
year = "2013",
abstract = "In recent years antioxidant properties of plant derived protein hydrolysates were established. Antioxidative activity of these hydrolysates largely depends on specificity of the proteinase used for hydrolysis, duration of proteinase treatment, as well as composition and molecular weight of hydrolysis derived peptides. In order to explore potential use of nine proteinase containing Lactobacillus strains in production of antioxidative peptides we tested their ability to hydrolyze lyophilized hydrosoluble protein fraction from dehulled and defatted dry pea seeds (Pisum sativum), cultivar NS Mraz. We also developed plant protein based liquid medium for cultivation of lactobacilli which was later used as starter for hydrolysis reaction in larger volume. We found that all nine strains could hydrolyze plant hydrosoluble fraction of pea seed proteins but with different efficiency. We also examined the effect of duration of hydrolysis on antioxidative activity in <10kDa fraction of obtained peptide extracts, using most proteolyticaly active strain, human vaginal isolate Lactobacillus rhamnosus T10. General conclusion was that antioxidative activity was positively correlated with increase of hydrolysis duration and amount of small peptides, which reached up to 34% of total proteins after 24h of hydrolysis treatment.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Application of different Lactobacillus strains in production pea seed protein hydrolysates with antioxidative activity",
pages = "286-286",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3040"
}

Stanisavljević, N., Vukotić, G., Fira, Đ., Jovanović, Ž., Miljuš-Đukić, J., Radović, S., Mikić, A.,& Maksimović, V.. (2013). Application of different Lactobacillus strains in production pea seed protein hydrolysates with antioxidative activity. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 286-286.
https://hdl.handle.net/21.15107/rcub_fiver_3040

Stanisavljević N, Vukotić G, Fira Đ, Jovanović Ž, Miljuš-Đukić J, Radović S, Mikić A, Maksimović V. Application of different Lactobacillus strains in production pea seed protein hydrolysates with antioxidative activity. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:286-286.
https://hdl.handle.net/21.15107/rcub_fiver_3040 .

Stanisavljević, Nemanja, Vukotić, Goran, Fira, Đorđe, Jovanović, Živko, Miljuš-Đukić, Jovanka, Radović, Svetlana, Mikić, Aleksandar, Maksimović, Vesna, "Application of different Lactobacillus strains in production pea seed protein hydrolysates with antioxidative activity" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):286-286,
https://hdl.handle.net/21.15107/rcub_fiver_3040 .

TY  - CONF
AU  - Radović, Svetlana
AU  - Mikić, Aleksandar
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Krstić, Đorđe
AU  - Ćupina, Branko
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3038
AB  - Pea is one of the most important crops in the world. Among the many ways of utilization of this crop are that related for forage production. Forage pea may be cultivated alone as a pure crop, or in mixture with other crops, usually cereals. In recent time, several attempts have been made to cultivate forage pea with other legumes, where pea would play the role of cover crop. The results reported here are obtained in a greenhouse experiment aimed to assess the pea as a companion crop for establishment of lucerne (Medicago sativa L.), red clover (Trifolium pratense L.) and sainfoin (Onobrychis sativa L.) as undersown crops. The semi-leafless pea cultivar Jezero with three population densities (30, 60 and 90 plants per m2) were used in this experiment. Two control treatments also included in the experiment – the perennial legumes grown as a pure stand, and the perennial legumes with oat as a cover crop. The effect of pea on forage yield, chlorophyll content, leaf surface and LER is determined. According to obtained results, the best combination for lucerne is 30 pea plants per m2, 90 for red clover and 60 pea plants per m2 for sainfoin.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Field pea (Pisum sativum L.) as a companion crop for the establishment of perennial legumes
EP  - 265
SP  - 265
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3038
ER  - 

@conference{
author = "Radović, Svetlana and Mikić, Aleksandar and Jovanović, Živko and Stanisavljević, Nemanja and Krstić, Đorđe and Ćupina, Branko",
year = "2013",
abstract = "Pea is one of the most important crops in the world. Among the many ways of utilization of this crop are that related for forage production. Forage pea may be cultivated alone as a pure crop, or in mixture with other crops, usually cereals. In recent time, several attempts have been made to cultivate forage pea with other legumes, where pea would play the role of cover crop. The results reported here are obtained in a greenhouse experiment aimed to assess the pea as a companion crop for establishment of lucerne (Medicago sativa L.), red clover (Trifolium pratense L.) and sainfoin (Onobrychis sativa L.) as undersown crops. The semi-leafless pea cultivar Jezero with three population densities (30, 60 and 90 plants per m2) were used in this experiment. Two control treatments also included in the experiment – the perennial legumes grown as a pure stand, and the perennial legumes with oat as a cover crop. The effect of pea on forage yield, chlorophyll content, leaf surface and LER is determined. According to obtained results, the best combination for lucerne is 30 pea plants per m2, 90 for red clover and 60 pea plants per m2 for sainfoin.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Field pea (Pisum sativum L.) as a companion crop for the establishment of perennial legumes",
pages = "265-265",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3038"
}

Radović, S., Mikić, A., Jovanović, Ž., Stanisavljević, N., Krstić, Đ.,& Ćupina, B.. (2013). Field pea (Pisum sativum L.) as a companion crop for the establishment of perennial legumes. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 265-265.
https://hdl.handle.net/21.15107/rcub_fiver_3038

Radović S, Mikić A, Jovanović Ž, Stanisavljević N, Krstić Đ, Ćupina B. Field pea (Pisum sativum L.) as a companion crop for the establishment of perennial legumes. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:265-265.
https://hdl.handle.net/21.15107/rcub_fiver_3038 .

Radović, Svetlana, Mikić, Aleksandar, Jovanović, Živko, Stanisavljević, Nemanja, Krstić, Đorđe, Ćupina, Branko, "Field pea (Pisum sativum L.) as a companion crop for the establishment of perennial legumes" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):265-265,
https://hdl.handle.net/21.15107/rcub_fiver_3038 .

TY  - CONF
AU  - Miljuš-Đukić, Jovanka
AU  - Stanisavljević, Nemanja
AU  - Radović, Svetlana
AU  - Jovanović, Živko
AU  - Mikić, Aleksandar
AU  - Maksimović, Vesna
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3024
AB  - Salinity is one of the major abiotic factors limiting plants productivity. Processes, such as seed germination, seedling growth and vigor, vegetative growth, flowering, and fruit set, are adversely affected by high saltconcentrations, ultimately causing diminished economic yield and also quality of products. Shoots of micropropagated Pisum fulvum, P. sativum and P. arvense were treated with 75, 120, 150 and 200 mMNaClin order to study pea tissues capacity to tolerate salt stress and their antioxidant capacity. We used the phenol content and DPPH antioxidant assay as oxidative damage indicators. Results showed that salt stress caused slower plant growth, leading to an increase in lipid peroxidation and a decrease in total chlorophyll in pea tissues. Further, results showed that P. fulvum is to be considered as potentially less sensitive to high NaCl concentrations in comparison to P. sativum and P. arvense and could represents a gene pool which could be used for improving the characteristics of field pea.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Salt stress response in three pea species (Pisum arvense, P. sativum and P. fulvum) in vitro cultures
EP  - 207
SP  - 207
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3024
ER  - 

@conference{
author = "Miljuš-Đukić, Jovanka and Stanisavljević, Nemanja and Radović, Svetlana and Jovanović, Živko and Mikić, Aleksandar and Maksimović, Vesna",
year = "2013",
abstract = "Salinity is one of the major abiotic factors limiting plants productivity. Processes, such as seed germination, seedling growth and vigor, vegetative growth, flowering, and fruit set, are adversely affected by high saltconcentrations, ultimately causing diminished economic yield and also quality of products. Shoots of micropropagated Pisum fulvum, P. sativum and P. arvense were treated with 75, 120, 150 and 200 mMNaClin order to study pea tissues capacity to tolerate salt stress and their antioxidant capacity. We used the phenol content and DPPH antioxidant assay as oxidative damage indicators. Results showed that salt stress caused slower plant growth, leading to an increase in lipid peroxidation and a decrease in total chlorophyll in pea tissues. Further, results showed that P. fulvum is to be considered as potentially less sensitive to high NaCl concentrations in comparison to P. sativum and P. arvense and could represents a gene pool which could be used for improving the characteristics of field pea.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Salt stress response in three pea species (Pisum arvense, P. sativum and P. fulvum) in vitro cultures",
pages = "207-207",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3024"
}

Miljuš-Đukić, J., Stanisavljević, N., Radović, S., Jovanović, Ž., Mikić, A.,& Maksimović, V.. (2013). Salt stress response in three pea species (Pisum arvense, P. sativum and P. fulvum) in vitro cultures. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 207-207.
https://hdl.handle.net/21.15107/rcub_fiver_3024

Miljuš-Đukić J, Stanisavljević N, Radović S, Jovanović Ž, Mikić A, Maksimović V. Salt stress response in three pea species (Pisum arvense, P. sativum and P. fulvum) in vitro cultures. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:207-207.
https://hdl.handle.net/21.15107/rcub_fiver_3024 .

Miljuš-Đukić, Jovanka, Stanisavljević, Nemanja, Radović, Svetlana, Jovanović, Živko, Mikić, Aleksandar, Maksimović, Vesna, "Salt stress response in three pea species (Pisum arvense, P. sativum and P. fulvum) in vitro cultures" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):207-207,
https://hdl.handle.net/21.15107/rcub_fiver_3024 .

TY  - CONF
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Miljuš-Đukić, Jovanka
AU  - Mikić, Aleksandar
AU  - Radović, Svetlana
AU  - Ćupina, Branko
AU  - Maksimović, Vesna
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3018
AB  - MicroRNAs (miRNAs), recently recognized as important regulator of gene expression at posttranscriptional level, have been found to be involved in plant stress responses. The observation that some miRNAs are up- or down regulated by stress implies that that could play vital roles in plant resistance to abiotic and biotic stress. We investigated the effect of water stress treatment during 10 days on expression of conserved miRNAs-miR398a/b and miR408 in pea plants. This time frame could reflect the changes as closely as possible those during water stress cause visible effects under field condition. It was observed that dehydration strongly down regulates the expression of both miR398a/b and miR408 in pea roots and shoots. The down-regulation of miR398a/b and the up-regulation of potential target genes copper superoxide dismutase, CSD1, highlight the involvement of this miRNA in pea stress response. On the contrary, the mRNA level of cytochrome c oxidase subunit 5 (COX5b) was not changed in roots and shoots of water-stressed plants, compared to control-well hydrated plants. This suggests that COX5b is not target of miR398, or that its expression is regulated by some other mechanism. P1B-ATPase expression increased during water deficit only in shoots of pea; in the roots there was no changes in expression. Our results might help to understand the possible role of investigated miRNAs and their contribution to pea capacity to cope with water deficit.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Dehydration affected the expression of miR398 and miR408 in pea (Pisum sativum L.)
EP  - 153
SP  - 153
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3018
ER  - 

@conference{
author = "Jovanović, Živko and Stanisavljević, Nemanja and Miljuš-Đukić, Jovanka and Mikić, Aleksandar and Radović, Svetlana and Ćupina, Branko and Maksimović, Vesna",
year = "2013",
abstract = "MicroRNAs (miRNAs), recently recognized as important regulator of gene expression at posttranscriptional level, have been found to be involved in plant stress responses. The observation that some miRNAs are up- or down regulated by stress implies that that could play vital roles in plant resistance to abiotic and biotic stress. We investigated the effect of water stress treatment during 10 days on expression of conserved miRNAs-miR398a/b and miR408 in pea plants. This time frame could reflect the changes as closely as possible those during water stress cause visible effects under field condition. It was observed that dehydration strongly down regulates the expression of both miR398a/b and miR408 in pea roots and shoots. The down-regulation of miR398a/b and the up-regulation of potential target genes copper superoxide dismutase, CSD1, highlight the involvement of this miRNA in pea stress response. On the contrary, the mRNA level of cytochrome c oxidase subunit 5 (COX5b) was not changed in roots and shoots of water-stressed plants, compared to control-well hydrated plants. This suggests that COX5b is not target of miR398, or that its expression is regulated by some other mechanism. P1B-ATPase expression increased during water deficit only in shoots of pea; in the roots there was no changes in expression. Our results might help to understand the possible role of investigated miRNAs and their contribution to pea capacity to cope with water deficit.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Dehydration affected the expression of miR398 and miR408 in pea (Pisum sativum L.)",
pages = "153-153",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3018"
}

Jovanović, Ž., Stanisavljević, N., Miljuš-Đukić, J., Mikić, A., Radović, S., Ćupina, B.,& Maksimović, V.. (2013). Dehydration affected the expression of miR398 and miR408 in pea (Pisum sativum L.). in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 153-153.
https://hdl.handle.net/21.15107/rcub_fiver_3018

Jovanović Ž, Stanisavljević N, Miljuš-Đukić J, Mikić A, Radović S, Ćupina B, Maksimović V. Dehydration affected the expression of miR398 and miR408 in pea (Pisum sativum L.). in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:153-153.
https://hdl.handle.net/21.15107/rcub_fiver_3018 .

Jovanović, Živko, Stanisavljević, Nemanja, Miljuš-Đukić, Jovanka, Mikić, Aleksandar, Radović, Svetlana, Ćupina, Branko, Maksimović, Vesna, "Dehydration affected the expression of miR398 and miR408 in pea (Pisum sativum L.)" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):153-153,
https://hdl.handle.net/21.15107/rcub_fiver_3018 .

TY  - CONF
AU  - Stanisavljević, Nemanja
AU  - Jovanović, Živko
AU  - Čupić, Tihomir
AU  - Lukić, Jovanka
AU  - Miljuš-Đukić, Jovanka
AU  - Radović, Svetlana
AU  - Mikić, Aleksandar
AU  - Maksimović, Vesna
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3007
AB  - Field pea (Pisum sativum var. arvense) and grass pea (Lathyrus sativus) are important high protein crops used as feed. Legume seeds are also rich in many other nutrient compounds including starch, sugar, dietary fiber and micronutrients (vitamins and minerals.) They are also a rich source of many bioactive non-nutrient compounds including phenolic antioxidants. Besides their beneficial effects, some phenolic compounds, especially condensed tannins in feed, are considered as antinutritional factors for ruminants, owing to their capacity for binding to macromolecules such as fiber and proteins. Seed mass and diameter are valuable parameters used in breeding, as well as seed mineral composition, since significant quantitative trait loci were identified for Ca, Fe, Mn, K, Mg, Ni, P and Zn. Thus we conducted this study in order to determine some parameters, in three cultivars of previously non-tested field pea and five grass pea cultivars from Croatia, which are important for breeders. We determined seed mass, diameter and proximate mineral composition as well as content of soluble sugars and total phenolic content in dry seeds of these cultivars. Cultivars differed significantly in total phenollics and mineral composition.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Analysis of some nutritional and antinutritional compounds in dry seeds of grass pea and field pea cultivars from Croatia
EP  - 104
SP  - 104
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3007
ER  - 

@conference{
author = "Stanisavljević, Nemanja and Jovanović, Živko and Čupić, Tihomir and Lukić, Jovanka and Miljuš-Đukić, Jovanka and Radović, Svetlana and Mikić, Aleksandar and Maksimović, Vesna",
year = "2013",
abstract = "Field pea (Pisum sativum var. arvense) and grass pea (Lathyrus sativus) are important high protein crops used as feed. Legume seeds are also rich in many other nutrient compounds including starch, sugar, dietary fiber and micronutrients (vitamins and minerals.) They are also a rich source of many bioactive non-nutrient compounds including phenolic antioxidants. Besides their beneficial effects, some phenolic compounds, especially condensed tannins in feed, are considered as antinutritional factors for ruminants, owing to their capacity for binding to macromolecules such as fiber and proteins. Seed mass and diameter are valuable parameters used in breeding, as well as seed mineral composition, since significant quantitative trait loci were identified for Ca, Fe, Mn, K, Mg, Ni, P and Zn. Thus we conducted this study in order to determine some parameters, in three cultivars of previously non-tested field pea and five grass pea cultivars from Croatia, which are important for breeders. We determined seed mass, diameter and proximate mineral composition as well as content of soluble sugars and total phenolic content in dry seeds of these cultivars. Cultivars differed significantly in total phenollics and mineral composition.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Analysis of some nutritional and antinutritional compounds in dry seeds of grass pea and field pea cultivars from Croatia",
pages = "104-104",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3007"
}

Stanisavljević, N., Jovanović, Ž., Čupić, T., Lukić, J., Miljuš-Đukić, J., Radović, S., Mikić, A.,& Maksimović, V.. (2013). Analysis of some nutritional and antinutritional compounds in dry seeds of grass pea and field pea cultivars from Croatia. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 104-104.
https://hdl.handle.net/21.15107/rcub_fiver_3007

Stanisavljević N, Jovanović Ž, Čupić T, Lukić J, Miljuš-Đukić J, Radović S, Mikić A, Maksimović V. Analysis of some nutritional and antinutritional compounds in dry seeds of grass pea and field pea cultivars from Croatia. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:104-104.
https://hdl.handle.net/21.15107/rcub_fiver_3007 .

Stanisavljević, Nemanja, Jovanović, Živko, Čupić, Tihomir, Lukić, Jovanka, Miljuš-Đukić, Jovanka, Radović, Svetlana, Mikić, Aleksandar, Maksimović, Vesna, "Analysis of some nutritional and antinutritional compounds in dry seeds of grass pea and field pea cultivars from Croatia" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):104-104,
https://hdl.handle.net/21.15107/rcub_fiver_3007 .

TY  - CONF
AU  - Stanisavljević, Nemanja
AU  - Jovanović, Živko
AU  - Čupić, Tihomir
AU  - Lukić, Jovanka
AU  - Miljuš-Đukić, Jovanka
AU  - Radović, Svetlana
AU  - Mikić, Aleksandar
AU  - Maksimović, Vesna
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/2998
AB  - Antioxidant activities and phenolic contents were studied in methanolic and water extracts of non-processed, cooked and in vitro enzymatically digested seed flour. Antioxidant activities of total protein hydrolysates and small peptide fractions (MW<3kDa and MW<3kDa) isolated from seed were also determined. Study showed that cooking and enzymatic digestion strongly enhanced release of phenolic compounds. Scavenging activity against 2,2-diphenyl-1-picryhydrazyl radical (DPPH•), hydroxyl radical and hydrogen peroxide were also increased. Protein hydrolysates especially MW<3kDa fraction possess significant antioxidative potential. Taken together our findings indicate that in methanolic and water extracts of cooked and digested seed flour small peptide fraction, beside phenolic compounds, can also significantly contribute to free radical and hydrogen peroxide scavenging activity.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Simple cooking and in vitro gastrointestinal digestion improves antioxidant activity of legume seed flour extracts
EP  - 63
SP  - 63
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2998
ER  - 

@conference{
author = "Stanisavljević, Nemanja and Jovanović, Živko and Čupić, Tihomir and Lukić, Jovanka and Miljuš-Đukić, Jovanka and Radović, Svetlana and Mikić, Aleksandar and Maksimović, Vesna",
year = "2013",
abstract = "Antioxidant activities and phenolic contents were studied in methanolic and water extracts of non-processed, cooked and in vitro enzymatically digested seed flour. Antioxidant activities of total protein hydrolysates and small peptide fractions (MW<3kDa and MW<3kDa) isolated from seed were also determined. Study showed that cooking and enzymatic digestion strongly enhanced release of phenolic compounds. Scavenging activity against 2,2-diphenyl-1-picryhydrazyl radical (DPPH•), hydroxyl radical and hydrogen peroxide were also increased. Protein hydrolysates especially MW<3kDa fraction possess significant antioxidative potential. Taken together our findings indicate that in methanolic and water extracts of cooked and digested seed flour small peptide fraction, beside phenolic compounds, can also significantly contribute to free radical and hydrogen peroxide scavenging activity.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Simple cooking and in vitro gastrointestinal digestion improves antioxidant activity of legume seed flour extracts",
pages = "63-63",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2998"
}

Stanisavljević, N., Jovanović, Ž., Čupić, T., Lukić, J., Miljuš-Đukić, J., Radović, S., Mikić, A.,& Maksimović, V.. (2013). Simple cooking and in vitro gastrointestinal digestion improves antioxidant activity of legume seed flour extracts. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 63-63.
https://hdl.handle.net/21.15107/rcub_fiver_2998

Stanisavljević N, Jovanović Ž, Čupić T, Lukić J, Miljuš-Đukić J, Radović S, Mikić A, Maksimović V. Simple cooking and in vitro gastrointestinal digestion improves antioxidant activity of legume seed flour extracts. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:63-63.
https://hdl.handle.net/21.15107/rcub_fiver_2998 .

Stanisavljević, Nemanja, Jovanović, Živko, Čupić, Tihomir, Lukić, Jovanka, Miljuš-Đukić, Jovanka, Radović, Svetlana, Mikić, Aleksandar, Maksimović, Vesna, "Simple cooking and in vitro gastrointestinal digestion improves antioxidant activity of legume seed flour extracts" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):63-63,
https://hdl.handle.net/21.15107/rcub_fiver_2998 .

TY  - CONF
AU  - Medović, Aleksandar
AU  - Smykal, Petr
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Zlatković, Bojan
AU  - Ćupina, Branko
AU  - Đorđević, Vuk
AU  - Mikić, Aleksandar
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/2983
AB  - A unique example of 2,572 carbonized pea seeds was recovered from the 11th century B.C. deposits at Hissar, a multilevel settlement of the Brnjica cultural group (1,350-1,000 B.C.) in southeast Serbia. Five hundred seeds maintained pea-like hilum, only few had preserved intact smooth-surfaced testa and the majority of seeds had no seed coat. Fortunately, only few seeds of other pulses were observed in the sample and their identity was clearly determined. Applying the exclusion principle, all the “naked”, 3-4 mm large seeds, mainly broad ellipsoid and less often globose, with flattenings or concavities, were determined to correspond to cultivated pea (Pisum sativum L.). Additionally, a high thousand seed weight of charred seeds (24.4 g) suggested cultivated status. To confirm our finding, we processed two samples with molecular tools. A sequence analysis of four chloroplast DNA loci (trnSG, trnK, matK and rbcL) in total length of 1329bp, showed intermediate position to cultivated P. sativum and wild P. sativum subsp. elatius.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - A combined archaeobotanical and palaeogenetic analysis of charred pea (Pisum sativum) seeds from an Early Iron Age storage pit at the hill fort settlement Hissar, Leskovac, southeast Serbia
EP  - 28
SP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2983
ER  - 

@conference{
author = "Medović, Aleksandar and Smykal, Petr and Jovanović, Živko and Stanisavljević, Nemanja and Zlatković, Bojan and Ćupina, Branko and Đorđević, Vuk and Mikić, Aleksandar",
year = "2013",
abstract = "A unique example of 2,572 carbonized pea seeds was recovered from the 11th century B.C. deposits at Hissar, a multilevel settlement of the Brnjica cultural group (1,350-1,000 B.C.) in southeast Serbia. Five hundred seeds maintained pea-like hilum, only few had preserved intact smooth-surfaced testa and the majority of seeds had no seed coat. Fortunately, only few seeds of other pulses were observed in the sample and their identity was clearly determined. Applying the exclusion principle, all the “naked”, 3-4 mm large seeds, mainly broad ellipsoid and less often globose, with flattenings or concavities, were determined to correspond to cultivated pea (Pisum sativum L.). Additionally, a high thousand seed weight of charred seeds (24.4 g) suggested cultivated status. To confirm our finding, we processed two samples with molecular tools. A sequence analysis of four chloroplast DNA loci (trnSG, trnK, matK and rbcL) in total length of 1329bp, showed intermediate position to cultivated P. sativum and wild P. sativum subsp. elatius.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "A combined archaeobotanical and palaeogenetic analysis of charred pea (Pisum sativum) seeds from an Early Iron Age storage pit at the hill fort settlement Hissar, Leskovac, southeast Serbia",
pages = "28-28",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2983"
}

Medović, A., Smykal, P., Jovanović, Ž., Stanisavljević, N., Zlatković, B., Ćupina, B., Đorđević, V.,& Mikić, A.. (2013). A combined archaeobotanical and palaeogenetic analysis of charred pea (Pisum sativum) seeds from an Early Iron Age storage pit at the hill fort settlement Hissar, Leskovac, southeast Serbia. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 28-28.
https://hdl.handle.net/21.15107/rcub_fiver_2983

Medović A, Smykal P, Jovanović Ž, Stanisavljević N, Zlatković B, Ćupina B, Đorđević V, Mikić A. A combined archaeobotanical and palaeogenetic analysis of charred pea (Pisum sativum) seeds from an Early Iron Age storage pit at the hill fort settlement Hissar, Leskovac, southeast Serbia. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:28-28.
https://hdl.handle.net/21.15107/rcub_fiver_2983 .

Medović, Aleksandar, Smykal, Petr, Jovanović, Živko, Stanisavljević, Nemanja, Zlatković, Bojan, Ćupina, Branko, Đorđević, Vuk, Mikić, Aleksandar, "A combined archaeobotanical and palaeogenetic analysis of charred pea (Pisum sativum) seeds from an Early Iron Age storage pit at the hill fort settlement Hissar, Leskovac, southeast Serbia" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):28-28,
https://hdl.handle.net/21.15107/rcub_fiver_2983 .