TY  - JOUR
AU  - Đukić, Nevena
AU  - Knežević, Desimir
AU  - Pantelić, Danijel
AU  - Živančev, Dragan
AU  - Torbica, Aleksandra
AU  - Marković, Stefan
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1842
AB  - The aim of our work was to examine the expression and accumulation of EF-Tu and eEF1A in grain filing stage of five genotypes of winter wheat and one oat genotype in conditions of heat stress. In addition, the correlation between accumulation of elongation factors eEF1A and EF-Tu, and yield components of cereals in the field was investigated. Flag leaf protein samples were analyzed by immunoblotting. Flag leaves were collected under conditions of moderate (23 degrees C; MT) and high air temperature (38 degrees C; HT) in a field experiment. After the harvest, grain yield was determined. The yield components, the weight of dry seed and grains number per spike, were assessed in the stage of full physiological maturity of investigated cultivars. Obtained results revealed a difference in the level of EF-Tu accumulation both under conditions of moderate air temperatures and conditions of heat stress among investigated cultivars. Cultivar Zvezdana was the only one that showed increase in EF-Tu accumulation under HT (25%) compared to MT. Immunoblot analysis indicated that the highest increase of eEF1A accumulation (43%) in relation to moderate temperature was detected in cultivar Talas. A significant, positive, linear correlation was found between the expression of eEF1A and small grains productivity under heatstress conditions.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - Expression of protein synthesis elongation factors in winter wheat and oat in response to heat stress
VL  - 240
DO  - 10.1016/j.jplph.2019.153015
ER  - 

@article{
author = "Đukić, Nevena and Knežević, Desimir and Pantelić, Danijel and Živančev, Dragan and Torbica, Aleksandra and Marković, Stefan",
year = "2019",
abstract = "The aim of our work was to examine the expression and accumulation of EF-Tu and eEF1A in grain filing stage of five genotypes of winter wheat and one oat genotype in conditions of heat stress. In addition, the correlation between accumulation of elongation factors eEF1A and EF-Tu, and yield components of cereals in the field was investigated. Flag leaf protein samples were analyzed by immunoblotting. Flag leaves were collected under conditions of moderate (23 degrees C; MT) and high air temperature (38 degrees C; HT) in a field experiment. After the harvest, grain yield was determined. The yield components, the weight of dry seed and grains number per spike, were assessed in the stage of full physiological maturity of investigated cultivars. Obtained results revealed a difference in the level of EF-Tu accumulation both under conditions of moderate air temperatures and conditions of heat stress among investigated cultivars. Cultivar Zvezdana was the only one that showed increase in EF-Tu accumulation under HT (25%) compared to MT. Immunoblot analysis indicated that the highest increase of eEF1A accumulation (43%) in relation to moderate temperature was detected in cultivar Talas. A significant, positive, linear correlation was found between the expression of eEF1A and small grains productivity under heatstress conditions.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "Expression of protein synthesis elongation factors in winter wheat and oat in response to heat stress",
volume = "240",
doi = "10.1016/j.jplph.2019.153015"
}

Đukić, N., Knežević, D., Pantelić, D., Živančev, D., Torbica, A.,& Marković, S.. (2019). Expression of protein synthesis elongation factors in winter wheat and oat in response to heat stress. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 240.
https://doi.org/10.1016/j.jplph.2019.153015

Đukić N, Knežević D, Pantelić D, Živančev D, Torbica A, Marković S. Expression of protein synthesis elongation factors in winter wheat and oat in response to heat stress. in Journal of Plant Physiology. 2019;240.
doi:10.1016/j.jplph.2019.153015 .

Đukić, Nevena, Knežević, Desimir, Pantelić, Danijel, Živančev, Dragan, Torbica, Aleksandra, Marković, Stefan, "Expression of protein synthesis elongation factors in winter wheat and oat in response to heat stress" in Journal of Plant Physiology, 240 (2019),
https://doi.org/10.1016/j.jplph.2019.153015 . .