TY  - JOUR
AU  - Torbica, Aleksandra
AU  - Horvat, Daniela
AU  - Živančev, Dragan
AU  - Belović, Miona
AU  - Simić, Gordana
AU  - Magdić, Damir
AU  - Đukić, Nevena
AU  - Dvojković, K.
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1666
AB  - The aim of this study was to compare efficiency of RP-HPLC (Reversed-Phase High-Performance Liquid Chromatography) and LOC (Lab-on-Chip) methods for wheat gluten protein quantification regarding clustering of wheat cultivars according to the genetic similarity (HMW-GS combinations), as well as to explore relations of these two methods to wheat quality parameters. For that purpose, wheat quality parameters (protein content, falling number, wet gluten content, gluten index, Farinograph, Extensograph, and Amylograph), as well as amounts of gliadin and glutenin fractions by RP-HPLC and LOC methods were determined in two different sets of wheat cultivars (Croatian and Serbian). The percentages of gluten proteins and the values of quality parameters were used to characterize the samples by principal component analysis (PCA). Gluten protein quantification performed by method based on the protein fraction separation by molecular weights (LOC) was better for grouping of genetically similar wheat cultivars than quantification of proteins separated by their different solubility in specified solvent gradient (RP-HPLC). LOC method showed higher potential in wheat quality prediction.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Alimentaria
T1  - Prediction of the genetic similarity of wheat and wheat quality by reversed-phase high-performance liquid chromatography and lab-on-chip methods
EP  - 144
IS  - 2
SP  - 137
VL  - 46
DO  - 10.1556/066.2016.0003
ER  - 

@article{
author = "Torbica, Aleksandra and Horvat, Daniela and Živančev, Dragan and Belović, Miona and Simić, Gordana and Magdić, Damir and Đukić, Nevena and Dvojković, K.",
year = "2017",
abstract = "The aim of this study was to compare efficiency of RP-HPLC (Reversed-Phase High-Performance Liquid Chromatography) and LOC (Lab-on-Chip) methods for wheat gluten protein quantification regarding clustering of wheat cultivars according to the genetic similarity (HMW-GS combinations), as well as to explore relations of these two methods to wheat quality parameters. For that purpose, wheat quality parameters (protein content, falling number, wet gluten content, gluten index, Farinograph, Extensograph, and Amylograph), as well as amounts of gliadin and glutenin fractions by RP-HPLC and LOC methods were determined in two different sets of wheat cultivars (Croatian and Serbian). The percentages of gluten proteins and the values of quality parameters were used to characterize the samples by principal component analysis (PCA). Gluten protein quantification performed by method based on the protein fraction separation by molecular weights (LOC) was better for grouping of genetically similar wheat cultivars than quantification of proteins separated by their different solubility in specified solvent gradient (RP-HPLC). LOC method showed higher potential in wheat quality prediction.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Alimentaria",
title = "Prediction of the genetic similarity of wheat and wheat quality by reversed-phase high-performance liquid chromatography and lab-on-chip methods",
pages = "144-137",
number = "2",
volume = "46",
doi = "10.1556/066.2016.0003"
}

Torbica, A., Horvat, D., Živančev, D., Belović, M., Simić, G., Magdić, D., Đukić, N.,& Dvojković, K.. (2017). Prediction of the genetic similarity of wheat and wheat quality by reversed-phase high-performance liquid chromatography and lab-on-chip methods. in Acta Alimentaria
Akademiai Kiado Zrt, Budapest., 46(2), 137-144.
https://doi.org/10.1556/066.2016.0003

Torbica A, Horvat D, Živančev D, Belović M, Simić G, Magdić D, Đukić N, Dvojković K. Prediction of the genetic similarity of wheat and wheat quality by reversed-phase high-performance liquid chromatography and lab-on-chip methods. in Acta Alimentaria. 2017;46(2):137-144.
doi:10.1556/066.2016.0003 .

Torbica, Aleksandra, Horvat, Daniela, Živančev, Dragan, Belović, Miona, Simić, Gordana, Magdić, Damir, Đukić, Nevena, Dvojković, K., "Prediction of the genetic similarity of wheat and wheat quality by reversed-phase high-performance liquid chromatography and lab-on-chip methods" in Acta Alimentaria, 46, no. 2 (2017):137-144,
https://doi.org/10.1556/066.2016.0003 . .

TY  - JOUR
AU  - Živančev, Dragan
AU  - Horvat, Daniela
AU  - Torbica, Aleksandra
AU  - Belović, Miona
AU  - Simić, Gordana
AU  - Magdić, Damir
AU  - Đukić, Nevena
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1480
AB  - RP-HPLC (reversed-phase high-performance liquid chromatography) is widely used to determine the amounts of the different gluten protein types. However, this method is time-consuming, especially at early stages of wheat breeding, when large number of samples needs to be analyzed. On the other hand, LoaC (Lab-on-a-Chip) technique has the potential for a fast, reliable, and automatable analysis of proteins. In the present study, benefits and limitations of Lab-on-a-Chip method over RP-HPLC method in gluten proteins evaluation were explored in order to determine in which way LoaC method should be improved in order to make its results more compliant with the results of RP-HPLC method. Strong correlation (P  lt = 0.001) was found between numbers of HMW glutenin peaks determined by LoaC and RP-HPLC methods. Significant correlations (P  lt = 0.05) were obtained between percentages of HMW and LMW glutenin subunits calculated with regard to total HMW + LMW area. Even more significant correlation (P  lt = 0.001) was found when percentages of individual HMW areas were calculated with regard to total HMW. RP-HPLC method showed superiority in determination of gliadins since larger number and better resolution of gliadin peaks were obtained by this method.
PB  - Hindawi Ltd, London
T2  - Journal of Chemistry
T1  - Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation
VL  - 2015
DO  - 10.1155/2015/430328
ER  - 

@article{
author = "Živančev, Dragan and Horvat, Daniela and Torbica, Aleksandra and Belović, Miona and Simić, Gordana and Magdić, Damir and Đukić, Nevena",
year = "2015",
abstract = "RP-HPLC (reversed-phase high-performance liquid chromatography) is widely used to determine the amounts of the different gluten protein types. However, this method is time-consuming, especially at early stages of wheat breeding, when large number of samples needs to be analyzed. On the other hand, LoaC (Lab-on-a-Chip) technique has the potential for a fast, reliable, and automatable analysis of proteins. In the present study, benefits and limitations of Lab-on-a-Chip method over RP-HPLC method in gluten proteins evaluation were explored in order to determine in which way LoaC method should be improved in order to make its results more compliant with the results of RP-HPLC method. Strong correlation (P  lt = 0.001) was found between numbers of HMW glutenin peaks determined by LoaC and RP-HPLC methods. Significant correlations (P  lt = 0.05) were obtained between percentages of HMW and LMW glutenin subunits calculated with regard to total HMW + LMW area. Even more significant correlation (P  lt = 0.001) was found when percentages of individual HMW areas were calculated with regard to total HMW. RP-HPLC method showed superiority in determination of gliadins since larger number and better resolution of gliadin peaks were obtained by this method.",
publisher = "Hindawi Ltd, London",
journal = "Journal of Chemistry",
title = "Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation",
volume = "2015",
doi = "10.1155/2015/430328"
}

Živančev, D., Horvat, D., Torbica, A., Belović, M., Simić, G., Magdić, D.,& Đukić, N.. (2015). Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation. in Journal of Chemistry
Hindawi Ltd, London., 2015.
https://doi.org/10.1155/2015/430328

Živančev D, Horvat D, Torbica A, Belović M, Simić G, Magdić D, Đukić N. Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation. in Journal of Chemistry. 2015;2015.
doi:10.1155/2015/430328 .

Živančev, Dragan, Horvat, Daniela, Torbica, Aleksandra, Belović, Miona, Simić, Gordana, Magdić, Damir, Đukić, Nevena, "Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation" in Journal of Chemistry, 2015 (2015),
https://doi.org/10.1155/2015/430328 . .

TY  - JOUR
AU  - Mastilović, Jasna
AU  - Horvat, Daniela
AU  - Živančev, Dragan
AU  - Torbica, Aleksandra
AU  - Kevrešan, Žarko S.
AU  - Đukić, Nevena
AU  - Magdić, Damir
AU  - Simić, Gordana
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1296
AB  - Data on protein fractions' proportion, obtained with RP-HPLC and technological quality parameters for 29 wheat cultivars grown in Serbia and Croatia, were used for studying of interrelations among wheat protein fractions with different solubility and molecular weight properties by multivariate (PCA) analysis. Obtained trends were used as the base for investigations related to differentiation of technological quality among wheat cultivars with different combination of protein fractions' compositions using univariate statistics (ANOVA followed by Duncan's test) in order to draw out information about interrelations between protein fractions proportion in wheat cultivars and their technological quality. Analysis based on the first four PCA factors (89.04% of variability) pointed out interdependencies between: 1) high content of albumins and globulins, low gliadins content and gliadins/glutenins ratio, high omega-gliadins, LMW glutenins and low alpha-gliadins share in total protein with low water absorption, high energy and high resistance to extensibility ratio, 2) high albumin and globulin content and high proportion of omega-gliadins in total protein and low extensigraph extensibility, 3) high share of high molecular weight glutenins (HMW-GS) in total proteins, high extensigraph resistance/extensibility ratio and 4) high gamma-gliadins share in total protein and low extensigraph resistance/extensibility ratio.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska Industrija
T1  - Analysis of interrelations between wheat protein fractions composition and its technological quality by combined multivariate and univariate statistics
EP  - 329
IS  - 3
SP  - 321
VL  - 68
DO  - 10.2298/HEMIND130313057M
ER  - 

@article{
author = "Mastilović, Jasna and Horvat, Daniela and Živančev, Dragan and Torbica, Aleksandra and Kevrešan, Žarko S. and Đukić, Nevena and Magdić, Damir and Simić, Gordana",
year = "2014",
abstract = "Data on protein fractions' proportion, obtained with RP-HPLC and technological quality parameters for 29 wheat cultivars grown in Serbia and Croatia, were used for studying of interrelations among wheat protein fractions with different solubility and molecular weight properties by multivariate (PCA) analysis. Obtained trends were used as the base for investigations related to differentiation of technological quality among wheat cultivars with different combination of protein fractions' compositions using univariate statistics (ANOVA followed by Duncan's test) in order to draw out information about interrelations between protein fractions proportion in wheat cultivars and their technological quality. Analysis based on the first four PCA factors (89.04% of variability) pointed out interdependencies between: 1) high content of albumins and globulins, low gliadins content and gliadins/glutenins ratio, high omega-gliadins, LMW glutenins and low alpha-gliadins share in total protein with low water absorption, high energy and high resistance to extensibility ratio, 2) high albumin and globulin content and high proportion of omega-gliadins in total protein and low extensigraph extensibility, 3) high share of high molecular weight glutenins (HMW-GS) in total proteins, high extensigraph resistance/extensibility ratio and 4) high gamma-gliadins share in total protein and low extensigraph resistance/extensibility ratio.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska Industrija",
title = "Analysis of interrelations between wheat protein fractions composition and its technological quality by combined multivariate and univariate statistics",
pages = "329-321",
number = "3",
volume = "68",
doi = "10.2298/HEMIND130313057M"
}

Mastilović, J., Horvat, D., Živančev, D., Torbica, A., Kevrešan, Ž. S., Đukić, N., Magdić, D.,& Simić, G.. (2014). Analysis of interrelations between wheat protein fractions composition and its technological quality by combined multivariate and univariate statistics. in Hemijska Industrija
Savez hemijskih inženjera, Beograd., 68(3), 321-329.
https://doi.org/10.2298/HEMIND130313057M

Mastilović J, Horvat D, Živančev D, Torbica A, Kevrešan ŽS, Đukić N, Magdić D, Simić G. Analysis of interrelations between wheat protein fractions composition and its technological quality by combined multivariate and univariate statistics. in Hemijska Industrija. 2014;68(3):321-329.
doi:10.2298/HEMIND130313057M .

Mastilović, Jasna, Horvat, Daniela, Živančev, Dragan, Torbica, Aleksandra, Kevrešan, Žarko S., Đukić, Nevena, Magdić, Damir, Simić, Gordana, "Analysis of interrelations between wheat protein fractions composition and its technological quality by combined multivariate and univariate statistics" in Hemijska Industrija, 68, no. 3 (2014):321-329,
https://doi.org/10.2298/HEMIND130313057M . .

TY  - JOUR
AU  - Horvat, Daniela
AU  - Đukić, Nevena
AU  - Magdić, Damir
AU  - Mastilović, Jasna
AU  - Simić, Gordana
AU  - Torbica, Aleksandra
AU  - Živančev, Dragan
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1252
AB  - Glutenin polymers composed of HMW and LMW subunits are important contributors to the wheat end-use properties. Twenty-six winter wheat cultivars differing in bread processing quality were collected at the experimental fields of the Agricultural Institute Osijek, Croatia and Institute of the Field and Vegetable Crops Novi Sad, Serbia, in 2008/2009 season. The HMW glutenins composition and glutenin proteins content were determined by SDS-PAGE and RP-HPLC, respectively, with aim to determine the relationship between glutenin protein fractions and wheat quality properties. Significant differences were found between Croatian and Serbian cultivars in several quality attributes (GI, WA, DDT, DS and R/EXT) as well as in the content of total glutenins and LMW glutenins and GLI/GLU ratio. The dominant HMW subunits in analyzed cultivars were 2*, 7 + 9/7 + 8 and 5 + 10. Principal component analysis (PCA) confirmed the presence of association between HMW glutenins composition and GI, dough E, R and R/EXT, while the glutenins quantitative data showed pronounced relation with P, DDT, DS, E, R and R/EXT. GLI/GLU ratio had the opposite effect on these parameters.
PB  - Springer Heidelberg, Heidelberg
T2  - Cereal Research Communications
T1  - Characterization of Bread Wheat Cultivars (Triticum aestivum L.) by Glutenin Proteins
EP  - 140
IS  - 1
SP  - 133
VL  - 41
DO  - 10.1556/CRC.2012.0021
ER  - 

@article{
author = "Horvat, Daniela and Đukić, Nevena and Magdić, Damir and Mastilović, Jasna and Simić, Gordana and Torbica, Aleksandra and Živančev, Dragan",
year = "2013",
abstract = "Glutenin polymers composed of HMW and LMW subunits are important contributors to the wheat end-use properties. Twenty-six winter wheat cultivars differing in bread processing quality were collected at the experimental fields of the Agricultural Institute Osijek, Croatia and Institute of the Field and Vegetable Crops Novi Sad, Serbia, in 2008/2009 season. The HMW glutenins composition and glutenin proteins content were determined by SDS-PAGE and RP-HPLC, respectively, with aim to determine the relationship between glutenin protein fractions and wheat quality properties. Significant differences were found between Croatian and Serbian cultivars in several quality attributes (GI, WA, DDT, DS and R/EXT) as well as in the content of total glutenins and LMW glutenins and GLI/GLU ratio. The dominant HMW subunits in analyzed cultivars were 2*, 7 + 9/7 + 8 and 5 + 10. Principal component analysis (PCA) confirmed the presence of association between HMW glutenins composition and GI, dough E, R and R/EXT, while the glutenins quantitative data showed pronounced relation with P, DDT, DS, E, R and R/EXT. GLI/GLU ratio had the opposite effect on these parameters.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Cereal Research Communications",
title = "Characterization of Bread Wheat Cultivars (Triticum aestivum L.) by Glutenin Proteins",
pages = "140-133",
number = "1",
volume = "41",
doi = "10.1556/CRC.2012.0021"
}

Horvat, D., Đukić, N., Magdić, D., Mastilović, J., Simić, G., Torbica, A.,& Živančev, D.. (2013). Characterization of Bread Wheat Cultivars (Triticum aestivum L.) by Glutenin Proteins. in Cereal Research Communications
Springer Heidelberg, Heidelberg., 41(1), 133-140.
https://doi.org/10.1556/CRC.2012.0021

Horvat D, Đukić N, Magdić D, Mastilović J, Simić G, Torbica A, Živančev D. Characterization of Bread Wheat Cultivars (Triticum aestivum L.) by Glutenin Proteins. in Cereal Research Communications. 2013;41(1):133-140.
doi:10.1556/CRC.2012.0021 .

Horvat, Daniela, Đukić, Nevena, Magdić, Damir, Mastilović, Jasna, Simić, Gordana, Torbica, Aleksandra, Živančev, Dragan, "Characterization of Bread Wheat Cultivars (Triticum aestivum L.) by Glutenin Proteins" in Cereal Research Communications, 41, no. 1 (2013):133-140,
https://doi.org/10.1556/CRC.2012.0021 . .