TY  - JOUR
AU  - Živančev, Dragan
AU  - Nikolovski, Branislava G.
AU  - Torbica, Aleksandra
AU  - Mastilović, Jasna
AU  - Đukić, Nevena
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1292
AB  - Polymeric wheat endosperm proteins, especially the high-molecular-weight glutenin subunits (HMW-GS), are probably the most interesting protein fraction giving the essential information about the bread-making quality of wheat flour. A relatively new method that shows great potential for a fast, reliable and automatable analysis of protein purity, sizing and quantification is microfluidic or Lab-on-a-Chip (LoaC) capillary electrophoresis. This aim of this work was to explore the possibilities of implementation of LoaC method to analysis of protein samples isolated from a Serbian common wheat variety, emphasizing the steps that might bring uncertainties and affect reproducibility of obtained glutenin subunits quantitation results. A good resolution of protein bands in a molecular weight range of 14.0 to 220.0 kDa was achieved. The reproducibility of HMW-GS sizing and quantitation were good, with the average coefficient of variation values of 1.2 and 12.2%. The ratio of HMW-GS to low-molecular-weight glutenin subunits (LMW-GS) was about 20%. The investigation ruled out influences of the extract solution addition and the buffer addition steps of the applied method, as well as the individual chip influence on GS quantitation results. However, there was statistically significant difference between HMW-GS quantitation results of multi-step and one-step extraction procedures applied prior to glutenin subunits extraction step.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - Lab-on-a-Chip method uncertainties in determination of high-molecular- weight glutenin subunits
EP  - 561
IS  - 4
SP  - 553
VL  - 19
DO  - 10.2298/CICEQ120517090Z
ER  - 

@article{
author = "Živančev, Dragan and Nikolovski, Branislava G. and Torbica, Aleksandra and Mastilović, Jasna and Đukić, Nevena",
year = "2013",
abstract = "Polymeric wheat endosperm proteins, especially the high-molecular-weight glutenin subunits (HMW-GS), are probably the most interesting protein fraction giving the essential information about the bread-making quality of wheat flour. A relatively new method that shows great potential for a fast, reliable and automatable analysis of protein purity, sizing and quantification is microfluidic or Lab-on-a-Chip (LoaC) capillary electrophoresis. This aim of this work was to explore the possibilities of implementation of LoaC method to analysis of protein samples isolated from a Serbian common wheat variety, emphasizing the steps that might bring uncertainties and affect reproducibility of obtained glutenin subunits quantitation results. A good resolution of protein bands in a molecular weight range of 14.0 to 220.0 kDa was achieved. The reproducibility of HMW-GS sizing and quantitation were good, with the average coefficient of variation values of 1.2 and 12.2%. The ratio of HMW-GS to low-molecular-weight glutenin subunits (LMW-GS) was about 20%. The investigation ruled out influences of the extract solution addition and the buffer addition steps of the applied method, as well as the individual chip influence on GS quantitation results. However, there was statistically significant difference between HMW-GS quantitation results of multi-step and one-step extraction procedures applied prior to glutenin subunits extraction step.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "Lab-on-a-Chip method uncertainties in determination of high-molecular- weight glutenin subunits",
pages = "561-553",
number = "4",
volume = "19",
doi = "10.2298/CICEQ120517090Z"
}

Živančev, D., Nikolovski, B. G., Torbica, A., Mastilović, J.,& Đukić, N.. (2013). Lab-on-a-Chip method uncertainties in determination of high-molecular- weight glutenin subunits. in Chemical Industry & Chemical Engineering Quarterly
Savez hemijskih inženjera, Beograd., 19(4), 553-561.
https://doi.org/10.2298/CICEQ120517090Z

Živančev D, Nikolovski BG, Torbica A, Mastilović J, Đukić N. Lab-on-a-Chip method uncertainties in determination of high-molecular- weight glutenin subunits. in Chemical Industry & Chemical Engineering Quarterly. 2013;19(4):553-561.
doi:10.2298/CICEQ120517090Z .

Živančev, Dragan, Nikolovski, Branislava G., Torbica, Aleksandra, Mastilović, Jasna, Đukić, Nevena, "Lab-on-a-Chip method uncertainties in determination of high-molecular- weight glutenin subunits" in Chemical Industry & Chemical Engineering Quarterly, 19, no. 4 (2013):553-561,
https://doi.org/10.2298/CICEQ120517090Z . .

TY  - JOUR
AU  - Torbica, Aleksandra
AU  - Živančev, Dragan
AU  - Nikolić, Zorica
AU  - Đorđević, Vuk
AU  - Nikolovski, Branislava G.
PY  - 2010
UR  - http://fiver.ifvcns.rs/handle/123456789/844
AB  - Qualitative and quantitative determination of the Kunitz trypsin inhibitor (KTI) in soybean experimental lines is very important in processes of selecting and breeding of new varieties. The total enzyme activity assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and Lab-on-a-Chip (LoaC) method were used for the determination of the presence and quantity of KTI in 15 soybean experimental lines and varieties. From the total trypsin inhibitor enzyme assay, inhibitor activities were registered in all samples, even in a Kunitz variety that was a negative control. The SDS-PAGE method did not detect the presence of the KTI protein band in seven soybean experimental lines and Kunitz variety, while the LoaC method showed the absence of KTI only in the Kunitz variety sample. Results confirmed the superiority of the LoaC method over other two methods in selectivity and sensitivity when KTI determination is concerned. Relationships between the KTI content obtained by the LoaC method and total trypsin inhibitor enzyme activity were established and statistically confirmed.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Agricultural & Food Chemistry
T1  - Advantages of the Lab-on-a-Chip Method in the Determination of the Kunitz Trypsin Inhibitor in Soybean Varieties
EP  - 7985
IS  - 13
SP  - 7980
VL  - 58
DO  - 10.1021/jf100830m
ER  - 

@article{
author = "Torbica, Aleksandra and Živančev, Dragan and Nikolić, Zorica and Đorđević, Vuk and Nikolovski, Branislava G.",
year = "2010",
abstract = "Qualitative and quantitative determination of the Kunitz trypsin inhibitor (KTI) in soybean experimental lines is very important in processes of selecting and breeding of new varieties. The total enzyme activity assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and Lab-on-a-Chip (LoaC) method were used for the determination of the presence and quantity of KTI in 15 soybean experimental lines and varieties. From the total trypsin inhibitor enzyme assay, inhibitor activities were registered in all samples, even in a Kunitz variety that was a negative control. The SDS-PAGE method did not detect the presence of the KTI protein band in seven soybean experimental lines and Kunitz variety, while the LoaC method showed the absence of KTI only in the Kunitz variety sample. Results confirmed the superiority of the LoaC method over other two methods in selectivity and sensitivity when KTI determination is concerned. Relationships between the KTI content obtained by the LoaC method and total trypsin inhibitor enzyme activity were established and statistically confirmed.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Agricultural & Food Chemistry",
title = "Advantages of the Lab-on-a-Chip Method in the Determination of the Kunitz Trypsin Inhibitor in Soybean Varieties",
pages = "7985-7980",
number = "13",
volume = "58",
doi = "10.1021/jf100830m"
}

Torbica, A., Živančev, D., Nikolić, Z., Đorđević, V.,& Nikolovski, B. G.. (2010). Advantages of the Lab-on-a-Chip Method in the Determination of the Kunitz Trypsin Inhibitor in Soybean Varieties. in Journal of Agricultural & Food Chemistry
Amer Chemical Soc, Washington., 58(13), 7980-7985.
https://doi.org/10.1021/jf100830m

Torbica A, Živančev D, Nikolić Z, Đorđević V, Nikolovski BG. Advantages of the Lab-on-a-Chip Method in the Determination of the Kunitz Trypsin Inhibitor in Soybean Varieties. in Journal of Agricultural & Food Chemistry. 2010;58(13):7980-7985.
doi:10.1021/jf100830m .

Torbica, Aleksandra, Živančev, Dragan, Nikolić, Zorica, Đorđević, Vuk, Nikolovski, Branislava G., "Advantages of the Lab-on-a-Chip Method in the Determination of the Kunitz Trypsin Inhibitor in Soybean Varieties" in Journal of Agricultural & Food Chemistry, 58, no. 13 (2010):7980-7985,
https://doi.org/10.1021/jf100830m . .