Krstović, Saša

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  • Krstović, Saša (4)
  • Krstović, Saša Z. (2)

Author's Bibliography

Changes in Fusarium and Aspergillus Mycotoxin Content and Fatty Acid Composition after the Application of Ozone in Different Maize Hybrids

Purar, Božana; Đalović, Ivica; Bekavac, Goran; Grahovac, Nada; Krstović, Saša; Latković, Dragana; Janić Hajnal, Elizabet; Živančev, Dragan

(Basel : MDPI, 2022)

TY  - JOUR
AU  - Purar, Božana
AU  - Đalović, Ivica
AU  - Bekavac, Goran
AU  - Grahovac, Nada
AU  - Krstović, Saša
AU  - Latković, Dragana
AU  - Janić Hajnal, Elizabet
AU  - Živančev, Dragan
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3206
AB  - Mycotoxins in maize represent a great threat to human health. For this reason, novel technics such as ozone treatment are used to reduce the content of maize mycotoxins. However, there is little knowledge about the effect of ozone treatment on maize quality parameters. This study investigated the changes in Fusarium and Aspergillus mycotoxins and the changes in fatty acids during the ozone treatment of maize samples. Sixteen maize hybrids were visually tested for the naturally occurring ear rot severity and treated with three different concentrations of ozone (40, 70, and 85 mg/L). Mycotoxin content in maize samples was determined using a high-performance liquid chromatography (HPLC) system, whereas dominant fatty acids were determined using gas chromatography coupled with a flame ionization detector (GC-FID). Ozone treatments could be successfully applied to reduce the content of mycotoxins in maize below the detection limit. Ozone treatments increased the content of monounsaturated fatty acids (MUFAs) and decreased the content of polyunsaturated fatty acids (PUFAs), i.e., linoleic acid (36.7% in relation to the lowest applied ozone concentration), which negatively affected the nutritional value of maize.
PB  - Basel : MDPI
T2  - Foods (Basel)
T1  - Changes in Fusarium and Aspergillus Mycotoxin Content and Fatty Acid Composition after the Application of Ozone in Different Maize Hybrids
IS  - 18
SP  - 2877
VL  - 11
DO  - 10.3390/foods11182877
ER  - 
@article{
author = "Purar, Božana and Đalović, Ivica and Bekavac, Goran and Grahovac, Nada and Krstović, Saša and Latković, Dragana and Janić Hajnal, Elizabet and Živančev, Dragan",
year = "2022",
abstract = "Mycotoxins in maize represent a great threat to human health. For this reason, novel technics such as ozone treatment are used to reduce the content of maize mycotoxins. However, there is little knowledge about the effect of ozone treatment on maize quality parameters. This study investigated the changes in Fusarium and Aspergillus mycotoxins and the changes in fatty acids during the ozone treatment of maize samples. Sixteen maize hybrids were visually tested for the naturally occurring ear rot severity and treated with three different concentrations of ozone (40, 70, and 85 mg/L). Mycotoxin content in maize samples was determined using a high-performance liquid chromatography (HPLC) system, whereas dominant fatty acids were determined using gas chromatography coupled with a flame ionization detector (GC-FID). Ozone treatments could be successfully applied to reduce the content of mycotoxins in maize below the detection limit. Ozone treatments increased the content of monounsaturated fatty acids (MUFAs) and decreased the content of polyunsaturated fatty acids (PUFAs), i.e., linoleic acid (36.7% in relation to the lowest applied ozone concentration), which negatively affected the nutritional value of maize.",
publisher = "Basel : MDPI",
journal = "Foods (Basel)",
title = "Changes in Fusarium and Aspergillus Mycotoxin Content and Fatty Acid Composition after the Application of Ozone in Different Maize Hybrids",
number = "18",
pages = "2877",
volume = "11",
doi = "10.3390/foods11182877"
}
Purar, B., Đalović, I., Bekavac, G., Grahovac, N., Krstović, S., Latković, D., Janić Hajnal, E.,& Živančev, D.. (2022). Changes in Fusarium and Aspergillus Mycotoxin Content and Fatty Acid Composition after the Application of Ozone in Different Maize Hybrids. in Foods (Basel)
Basel : MDPI., 11(18), 2877.
https://doi.org/10.3390/foods11182877
Purar B, Đalović I, Bekavac G, Grahovac N, Krstović S, Latković D, Janić Hajnal E, Živančev D. Changes in Fusarium and Aspergillus Mycotoxin Content and Fatty Acid Composition after the Application of Ozone in Different Maize Hybrids. in Foods (Basel). 2022;11(18):2877.
doi:10.3390/foods11182877 .
Purar, Božana, Đalović, Ivica, Bekavac, Goran, Grahovac, Nada, Krstović, Saša, Latković, Dragana, Janić Hajnal, Elizabet, Živančev, Dragan, "Changes in Fusarium and Aspergillus Mycotoxin Content and Fatty Acid Composition after the Application of Ozone in Different Maize Hybrids" in Foods (Basel), 11, no. 18 (2022):2877,
https://doi.org/10.3390/foods11182877 . .
3
3

Biological Control of Aflatoxin in Maize Grown in Serbia

Savić, Zagorka; Dudaš, Tatjana; Loc, Marta; Grahovac, Mila; Budakov, Dragana; Jajić, Igor; Krstović, Saša; Barošević, Tijana; Krska, Rudolf; Sulyok, Michael; Stojšin, Vera; Petreš, Mladen; Stankov, Aleksandra; Vukotić, Jelena; Bagi, Ferenc

(Basel : MDPI, 2020)

TY  - JOUR
AU  - Savić, Zagorka
AU  - Dudaš, Tatjana
AU  - Loc, Marta
AU  - Grahovac, Mila
AU  - Budakov, Dragana
AU  - Jajić, Igor
AU  - Krstović, Saša
AU  - Barošević, Tijana
AU  - Krska, Rudolf
AU  - Sulyok, Michael
AU  - Stojšin, Vera
AU  - Petreš, Mladen
AU  - Stankov, Aleksandra
AU  - Vukotić, Jelena
AU  - Bagi, Ferenc
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/4355
AB  - Aspergillus flavus is the main producer of aflatoxin B1, one of the most toxic contaminants
of food and feed. With global warming, climate conditions have become favourable for aflatoxin
contamination of agricultural products in several European countries, including Serbia. The
infection of maize with A. flavus, and aflatoxin synthesis can be controlled and reduced by
application of a biocontrol product based on non‐toxigenic strains of A. flavus. Biological control
relies on competition between atoxigenic and toxigenic strains. This is the most commonly used
biological control mechanism of aflatoxin contamination in maize in countries where aflatoxins
pose a significant threat. Mytoolbox Af01, a native atoxigenic A. flavus strain, was obtained from
maize grown in Serbia and used to produce a biocontrol product that was applied in irrigated and
non‐irrigated Serbian fields during 2016 and 2017. The application of this biocontrol product
reduced aflatoxin levels in maize kernels (51–83%). The biocontrol treatment had a highly
significant effect of reducing total aflatoxin contamination by 73%. This study showed that
aflatoxin contamination control in Serbian maize can be achieved through biological control
methods using atoxigenic A. flavus strains.
PB  - Basel : MDPI
T2  - Toxins
T1  - Biological Control of Aflatoxin in Maize Grown in Serbia
IS  - 3
SP  - 162
VL  - 12
DO  - 10.3390/toxins12030162
ER  - 
@article{
author = "Savić, Zagorka and Dudaš, Tatjana and Loc, Marta and Grahovac, Mila and Budakov, Dragana and Jajić, Igor and Krstović, Saša and Barošević, Tijana and Krska, Rudolf and Sulyok, Michael and Stojšin, Vera and Petreš, Mladen and Stankov, Aleksandra and Vukotić, Jelena and Bagi, Ferenc",
year = "2020",
abstract = "Aspergillus flavus is the main producer of aflatoxin B1, one of the most toxic contaminants
of food and feed. With global warming, climate conditions have become favourable for aflatoxin
contamination of agricultural products in several European countries, including Serbia. The
infection of maize with A. flavus, and aflatoxin synthesis can be controlled and reduced by
application of a biocontrol product based on non‐toxigenic strains of A. flavus. Biological control
relies on competition between atoxigenic and toxigenic strains. This is the most commonly used
biological control mechanism of aflatoxin contamination in maize in countries where aflatoxins
pose a significant threat. Mytoolbox Af01, a native atoxigenic A. flavus strain, was obtained from
maize grown in Serbia and used to produce a biocontrol product that was applied in irrigated and
non‐irrigated Serbian fields during 2016 and 2017. The application of this biocontrol product
reduced aflatoxin levels in maize kernels (51–83%). The biocontrol treatment had a highly
significant effect of reducing total aflatoxin contamination by 73%. This study showed that
aflatoxin contamination control in Serbian maize can be achieved through biological control
methods using atoxigenic A. flavus strains.",
publisher = "Basel : MDPI",
journal = "Toxins",
title = "Biological Control of Aflatoxin in Maize Grown in Serbia",
number = "3",
pages = "162",
volume = "12",
doi = "10.3390/toxins12030162"
}
Savić, Z., Dudaš, T., Loc, M., Grahovac, M., Budakov, D., Jajić, I., Krstović, S., Barošević, T., Krska, R., Sulyok, M., Stojšin, V., Petreš, M., Stankov, A., Vukotić, J.,& Bagi, F.. (2020). Biological Control of Aflatoxin in Maize Grown in Serbia. in Toxins
Basel : MDPI., 12(3), 162.
https://doi.org/10.3390/toxins12030162
Savić Z, Dudaš T, Loc M, Grahovac M, Budakov D, Jajić I, Krstović S, Barošević T, Krska R, Sulyok M, Stojšin V, Petreš M, Stankov A, Vukotić J, Bagi F. Biological Control of Aflatoxin in Maize Grown in Serbia. in Toxins. 2020;12(3):162.
doi:10.3390/toxins12030162 .
Savić, Zagorka, Dudaš, Tatjana, Loc, Marta, Grahovac, Mila, Budakov, Dragana, Jajić, Igor, Krstović, Saša, Barošević, Tijana, Krska, Rudolf, Sulyok, Michael, Stojšin, Vera, Petreš, Mladen, Stankov, Aleksandra, Vukotić, Jelena, Bagi, Ferenc, "Biological Control of Aflatoxin in Maize Grown in Serbia" in Toxins, 12, no. 3 (2020):162,
https://doi.org/10.3390/toxins12030162 . .
1
46

The effect of genotype (G), growing environment (E), and their interaction (G x E) on the chemical composition of wheat bran

Kondić-Špika, Ankica; Mikić, Sanja; Trkulja, Dragana; Mladenov, Novica; Grahovac, Nada; Sakač, Zvonimir; Krstović, Saša; Hristov, Nikola

(Novi Sad : University of Novi Sad, Faculty of Technology, 2019)

TY  - CONF
AU  - Kondić-Špika, Ankica
AU  - Mikić, Sanja
AU  - Trkulja, Dragana
AU  - Mladenov, Novica
AU  - Grahovac, Nada
AU  - Sakač, Zvonimir
AU  - Krstović, Saša
AU  - Hristov, Nikola
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/2206
AB  - Wheat bran is a rich source of dietary fibres, some of essential fatty acids and contains important quantities of starch, protein, vitamins, minerals and phytic acid. It is also well known for its powerful anticarcinogenic and antioxidant properties. Field experiments with a collection of 23 wheat genotypes were conducted during two growing seasons in order to evaluate the effects of genotype (G), growing environment (E) and their interaction (G x E) on the nutrient composition of wheat bran. The samples of the bran portion of wheat grain were obtained as by-products of milling on the MLU 202 laboratory flour mill. Oil content was obtained by exhaustively extracting samples in a soxhlet apparatus using petroleum ether as the extractant. Tocopherols were determined by using extraction with hexane and normal-phase high- performance liquid chromatography with detection in fluorescent region. The mobile phase consisted a mixture of hexane:ethy] acetate (70:30, v/v). The results have shown that all sources of variation significantly affected chemical composition of wheat bran fraction. Total oil contents of wheat bran varied between 3.03-5.37% in the first and between 2.68-4.96% in the second growing season for investigated genotypes. Gas chromatography analyses of wheat bran oil showed major fatty acids to be linoleic, oleic and linolenic acids with wider range of variation expressed in the second growing season. Total tocopherols content in wheat bran extracts ranged from 16.3 to 77.6 mg/kg in the first and from 28.08 to 107.49 mg/kg in the second growing season. Significant differences in oil content and its chemical composition were found among the genotypes, indicating the great genetic potential for improvement. Also, the effect of the environment on all investigated compounds was highly significant, indicating the possibility to produce wheat with high levels of selected health components and antioxidant activities in a specific growing environment.
PB  - Novi Sad : University of Novi Sad, Faculty of Technology
C3  - Book of Abstracts, 1st International Conference on Advanced Production and Processing, 10-11 October 2019, Novi Sad
T1  - The effect of genotype (G), growing environment (E), and their interaction (G x E) on the chemical composition of wheat bran
EP  - 13
SP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2206
ER  - 
@conference{
author = "Kondić-Špika, Ankica and Mikić, Sanja and Trkulja, Dragana and Mladenov, Novica and Grahovac, Nada and Sakač, Zvonimir and Krstović, Saša and Hristov, Nikola",
year = "2019",
abstract = "Wheat bran is a rich source of dietary fibres, some of essential fatty acids and contains important quantities of starch, protein, vitamins, minerals and phytic acid. It is also well known for its powerful anticarcinogenic and antioxidant properties. Field experiments with a collection of 23 wheat genotypes were conducted during two growing seasons in order to evaluate the effects of genotype (G), growing environment (E) and their interaction (G x E) on the nutrient composition of wheat bran. The samples of the bran portion of wheat grain were obtained as by-products of milling on the MLU 202 laboratory flour mill. Oil content was obtained by exhaustively extracting samples in a soxhlet apparatus using petroleum ether as the extractant. Tocopherols were determined by using extraction with hexane and normal-phase high- performance liquid chromatography with detection in fluorescent region. The mobile phase consisted a mixture of hexane:ethy] acetate (70:30, v/v). The results have shown that all sources of variation significantly affected chemical composition of wheat bran fraction. Total oil contents of wheat bran varied between 3.03-5.37% in the first and between 2.68-4.96% in the second growing season for investigated genotypes. Gas chromatography analyses of wheat bran oil showed major fatty acids to be linoleic, oleic and linolenic acids with wider range of variation expressed in the second growing season. Total tocopherols content in wheat bran extracts ranged from 16.3 to 77.6 mg/kg in the first and from 28.08 to 107.49 mg/kg in the second growing season. Significant differences in oil content and its chemical composition were found among the genotypes, indicating the great genetic potential for improvement. Also, the effect of the environment on all investigated compounds was highly significant, indicating the possibility to produce wheat with high levels of selected health components and antioxidant activities in a specific growing environment.",
publisher = "Novi Sad : University of Novi Sad, Faculty of Technology",
journal = "Book of Abstracts, 1st International Conference on Advanced Production and Processing, 10-11 October 2019, Novi Sad",
title = "The effect of genotype (G), growing environment (E), and their interaction (G x E) on the chemical composition of wheat bran",
pages = "13-13",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2206"
}
Kondić-Špika, A., Mikić, S., Trkulja, D., Mladenov, N., Grahovac, N., Sakač, Z., Krstović, S.,& Hristov, N.. (2019). The effect of genotype (G), growing environment (E), and their interaction (G x E) on the chemical composition of wheat bran. in Book of Abstracts, 1st International Conference on Advanced Production and Processing, 10-11 October 2019, Novi Sad
Novi Sad : University of Novi Sad, Faculty of Technology., 13-13.
https://hdl.handle.net/21.15107/rcub_fiver_2206
Kondić-Špika A, Mikić S, Trkulja D, Mladenov N, Grahovac N, Sakač Z, Krstović S, Hristov N. The effect of genotype (G), growing environment (E), and their interaction (G x E) on the chemical composition of wheat bran. in Book of Abstracts, 1st International Conference on Advanced Production and Processing, 10-11 October 2019, Novi Sad. 2019;:13-13.
https://hdl.handle.net/21.15107/rcub_fiver_2206 .
Kondić-Špika, Ankica, Mikić, Sanja, Trkulja, Dragana, Mladenov, Novica, Grahovac, Nada, Sakač, Zvonimir, Krstović, Saša, Hristov, Nikola, "The effect of genotype (G), growing environment (E), and their interaction (G x E) on the chemical composition of wheat bran" in Book of Abstracts, 1st International Conference on Advanced Production and Processing, 10-11 October 2019, Novi Sad (2019):13-13,
https://hdl.handle.net/21.15107/rcub_fiver_2206 .

The composition of fatty acids and tocopherols in wheat bran oil

Kondić-Špika, Ankica; Grahovac, Nada; Sakač, Zvonimir; Mikić, Sanja; Trkulja, Dragana; Krstović, Saša; Hristov, Nikola

(Belgrade : Serbian Plant Physiology Society, 2018)

TY  - CONF
AU  - Kondić-Špika, Ankica
AU  - Grahovac, Nada
AU  - Sakač, Zvonimir
AU  - Mikić, Sanja
AU  - Trkulja, Dragana
AU  - Krstović, Saša
AU  - Hristov, Nikola
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/2257
AB  - Wheat is a significant component in human diet. The health effects of whole kernel utilization have been associated with their unique phytochemical compounds distributed in the endosperm, germ and bran fractions. The wheat brans represent rich source of tocopherols and essential fatty acids, such as linoleic acid (18:2n6) and linolenic acid (18:3n3). The aim of this study was to analyse tocopherol and fatty acid contents in bran oil of 17 wheat cultivars in order to identify genotypes with higher nutritional value. The oil was extracted from wheat bran using classical Rushkovsky method. Normal phase high performance liquid chromatography (NP-HPLC) with fluorescence detection was used for identification and quantitation of tocopherols. Trimethylsilyl esters of fatty acids were determined from wheat bran oils by using gas chromatography with a flame ionisation detector (GC-FID). Total tocopherol content ranged from 40.86 mg kg-1 (cv. NS rana 5) to 124.85 mg kg-1 (cv. Partizanka), with average content of 22.9 mg kg-1 α- tocopherols, 8 mg kg-1 β-tocopherols and 47 mg kg-1 γ- tocopherols. Cvs. Partizanka and NS 40S were identified as genotypes with significantly higher than average contents of all tocopherols. The content of 18:2n6, oleic and 18:3n3 acids were within the ranges of 55.85-73.05%, 9.09-24.82% and 3.63-7.81% of the total fatty acids, respectively. Our results showed that contents of both tocopherol and essential fatty acids (18:2n6 and 18:3n3) varied significantly in the investigated cultivars, suggesting that it is feasible to breed wheat cultivars with increased levels of beneficial phytochemicals for human health.
PB  - Belgrade : Serbian Plant Physiology Society
PB  - Belgrade : University of Belgrade, Institute for Biological Research “Siniša  Stanković“
PB  - Belgrade : University of Belgrade, Faculty of Biology
C3  - Book of Abstracts, 3rd International Conference on Plant Biology, 9-12 June 2018, Belgrade, Serbia
T1  - The composition of fatty acids and tocopherols in wheat bran oil
EP  - 138
SP  - 138
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2257
ER  - 
@conference{
author = "Kondić-Špika, Ankica and Grahovac, Nada and Sakač, Zvonimir and Mikić, Sanja and Trkulja, Dragana and Krstović, Saša and Hristov, Nikola",
year = "2018",
abstract = "Wheat is a significant component in human diet. The health effects of whole kernel utilization have been associated with their unique phytochemical compounds distributed in the endosperm, germ and bran fractions. The wheat brans represent rich source of tocopherols and essential fatty acids, such as linoleic acid (18:2n6) and linolenic acid (18:3n3). The aim of this study was to analyse tocopherol and fatty acid contents in bran oil of 17 wheat cultivars in order to identify genotypes with higher nutritional value. The oil was extracted from wheat bran using classical Rushkovsky method. Normal phase high performance liquid chromatography (NP-HPLC) with fluorescence detection was used for identification and quantitation of tocopherols. Trimethylsilyl esters of fatty acids were determined from wheat bran oils by using gas chromatography with a flame ionisation detector (GC-FID). Total tocopherol content ranged from 40.86 mg kg-1 (cv. NS rana 5) to 124.85 mg kg-1 (cv. Partizanka), with average content of 22.9 mg kg-1 α- tocopherols, 8 mg kg-1 β-tocopherols and 47 mg kg-1 γ- tocopherols. Cvs. Partizanka and NS 40S were identified as genotypes with significantly higher than average contents of all tocopherols. The content of 18:2n6, oleic and 18:3n3 acids were within the ranges of 55.85-73.05%, 9.09-24.82% and 3.63-7.81% of the total fatty acids, respectively. Our results showed that contents of both tocopherol and essential fatty acids (18:2n6 and 18:3n3) varied significantly in the investigated cultivars, suggesting that it is feasible to breed wheat cultivars with increased levels of beneficial phytochemicals for human health.",
publisher = "Belgrade : Serbian Plant Physiology Society, Belgrade : University of Belgrade, Institute for Biological Research “Siniša  Stanković“, Belgrade : University of Belgrade, Faculty of Biology",
journal = "Book of Abstracts, 3rd International Conference on Plant Biology, 9-12 June 2018, Belgrade, Serbia",
title = "The composition of fatty acids and tocopherols in wheat bran oil",
pages = "138-138",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2257"
}
Kondić-Špika, A., Grahovac, N., Sakač, Z., Mikić, S., Trkulja, D., Krstović, S.,& Hristov, N.. (2018). The composition of fatty acids and tocopherols in wheat bran oil. in Book of Abstracts, 3rd International Conference on Plant Biology, 9-12 June 2018, Belgrade, Serbia
Belgrade : Serbian Plant Physiology Society., 138-138.
https://hdl.handle.net/21.15107/rcub_fiver_2257
Kondić-Špika A, Grahovac N, Sakač Z, Mikić S, Trkulja D, Krstović S, Hristov N. The composition of fatty acids and tocopherols in wheat bran oil. in Book of Abstracts, 3rd International Conference on Plant Biology, 9-12 June 2018, Belgrade, Serbia. 2018;:138-138.
https://hdl.handle.net/21.15107/rcub_fiver_2257 .
Kondić-Špika, Ankica, Grahovac, Nada, Sakač, Zvonimir, Mikić, Sanja, Trkulja, Dragana, Krstović, Saša, Hristov, Nikola, "The composition of fatty acids and tocopherols in wheat bran oil" in Book of Abstracts, 3rd International Conference on Plant Biology, 9-12 June 2018, Belgrade, Serbia (2018):138-138,
https://hdl.handle.net/21.15107/rcub_fiver_2257 .

Presence of zearalenone in the most commonly grown wheat cultivars in Serbia

Jajić, Igor; Krstović, Saša Z.; Perišić, Biljana Č.; Jakšić, Sandra M.; Bursić, Vojislava; Jevtić, Radivoje; Abramović, Biljana F.

(Matica srpska, Novi Sad, 2013)

TY  - JOUR
AU  - Jajić, Igor
AU  - Krstović, Saša Z.
AU  - Perišić, Biljana Č.
AU  - Jakšić, Sandra M.
AU  - Bursić, Vojislava
AU  - Jevtić, Radivoje
AU  - Abramović, Biljana F.
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1276
AB  - A total of 45 samples of wheat from three different locations in Vojvodina were analyzed for the presence of zearalenone. Analytical methods based on clean-up by solid-phase extraction (SPE) columns and detection by liquid chromatography were used after validation. Limit of detection for ZEA in wheat was 18.6 μg/kg and the limit of quantification was 56.5 μg/kg. Recovery values ranged between 86% and 97%. The occurrence of ZEA in wheat was rather high with 53.3% of positive samples with the average value of 330 μg/kg. Incidences were found from 68 μg/kg to 1079 μg/kg. Contamination levels were above the established maximum limit for unprocessed cereals, other than maize, in as many as seventeen samples. These results were compared to the results of investigation of deoxynivalenol and fumonisin content, established in our previous work on the same samples. The results obtained were also compared to those of the neighboring countries where the relevant data existed and to the data of previous studies in our country.
AB  - Na prisustvo zearalenona analizirano je ukupno 45 uzoraka pšenice sa tri različite lokacije u Vojvodini. Korišćene su analitičke metode zasnovane na prečišćavanju ekstrakcijom na čvrstoj fazi, te kvantifikacija tečnom hromatografijom, nakon validacije metode. Granica detekcije za zearalenon u pšenici je iznosila 18,6 μg/kg, a granica određivanja 56,5 μg/kg. Efikasnost metode je bila u opsegu od 86% do 97%. Zearalenon je bio prisutan u 53,3% ispitivanih uzoraka, sa prosečnim sadržajem od 330 μg/kg. Dobijene vrednosti sadržaja zearalenona su bile u opsegu od 68 μg/kg do 1079 μg/kg. U čak sedamnaest uzoraka je pronađena koncentracija ovog toksina koja prevazilazi maksimalni dozvoljeni sadržaj zearalenona u netretiranim žitaricama. Ovi rezultati su upoređeni sa vrednostima sadržaja deoksinivalenola i fumonizina u istim uzorcima dobijenim u našim prethodnim istraživanjima. Rezultati su takođe upoređeni sa dostupnim rezultatima dobijenim u našoj i susednim zemljama tokom prethodnih godina.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Presence of zearalenone in the most commonly grown wheat cultivars in Serbia
T1  - Prisustvo zearalenona u najčešće uzgajanim sortama pšenice u Srbiji
EP  - 109
IS  - 124
SP  - 101
DO  - 10.2298/ZMSPN1324101J
ER  - 
@article{
author = "Jajić, Igor and Krstović, Saša Z. and Perišić, Biljana Č. and Jakšić, Sandra M. and Bursić, Vojislava and Jevtić, Radivoje and Abramović, Biljana F.",
year = "2013",
abstract = "A total of 45 samples of wheat from three different locations in Vojvodina were analyzed for the presence of zearalenone. Analytical methods based on clean-up by solid-phase extraction (SPE) columns and detection by liquid chromatography were used after validation. Limit of detection for ZEA in wheat was 18.6 μg/kg and the limit of quantification was 56.5 μg/kg. Recovery values ranged between 86% and 97%. The occurrence of ZEA in wheat was rather high with 53.3% of positive samples with the average value of 330 μg/kg. Incidences were found from 68 μg/kg to 1079 μg/kg. Contamination levels were above the established maximum limit for unprocessed cereals, other than maize, in as many as seventeen samples. These results were compared to the results of investigation of deoxynivalenol and fumonisin content, established in our previous work on the same samples. The results obtained were also compared to those of the neighboring countries where the relevant data existed and to the data of previous studies in our country., Na prisustvo zearalenona analizirano je ukupno 45 uzoraka pšenice sa tri različite lokacije u Vojvodini. Korišćene su analitičke metode zasnovane na prečišćavanju ekstrakcijom na čvrstoj fazi, te kvantifikacija tečnom hromatografijom, nakon validacije metode. Granica detekcije za zearalenon u pšenici je iznosila 18,6 μg/kg, a granica određivanja 56,5 μg/kg. Efikasnost metode je bila u opsegu od 86% do 97%. Zearalenon je bio prisutan u 53,3% ispitivanih uzoraka, sa prosečnim sadržajem od 330 μg/kg. Dobijene vrednosti sadržaja zearalenona su bile u opsegu od 68 μg/kg do 1079 μg/kg. U čak sedamnaest uzoraka je pronađena koncentracija ovog toksina koja prevazilazi maksimalni dozvoljeni sadržaj zearalenona u netretiranim žitaricama. Ovi rezultati su upoređeni sa vrednostima sadržaja deoksinivalenola i fumonizina u istim uzorcima dobijenim u našim prethodnim istraživanjima. Rezultati su takođe upoređeni sa dostupnim rezultatima dobijenim u našoj i susednim zemljama tokom prethodnih godina.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Presence of zearalenone in the most commonly grown wheat cultivars in Serbia, Prisustvo zearalenona u najčešće uzgajanim sortama pšenice u Srbiji",
pages = "109-101",
number = "124",
doi = "10.2298/ZMSPN1324101J"
}
Jajić, I., Krstović, S. Z., Perišić, B. Č., Jakšić, S. M., Bursić, V., Jevtić, R.,& Abramović, B. F.. (2013). Presence of zearalenone in the most commonly grown wheat cultivars in Serbia. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(124), 101-109.
https://doi.org/10.2298/ZMSPN1324101J
Jajić I, Krstović SZ, Perišić BČ, Jakšić SM, Bursić V, Jevtić R, Abramović BF. Presence of zearalenone in the most commonly grown wheat cultivars in Serbia. in Zbornik Matice srpske za prirodne nauke. 2013;(124):101-109.
doi:10.2298/ZMSPN1324101J .
Jajić, Igor, Krstović, Saša Z., Perišić, Biljana Č., Jakšić, Sandra M., Bursić, Vojislava, Jevtić, Radivoje, Abramović, Biljana F., "Presence of zearalenone in the most commonly grown wheat cultivars in Serbia" in Zbornik Matice srpske za prirodne nauke, no. 124 (2013):101-109,
https://doi.org/10.2298/ZMSPN1324101J . .
2

Presence of deoxynivalenol in small-grain samples from 2009/10 harvest season

Jajić, Igor; Jevtić, Radivoje; Jurić, Verica B.; Krstović, Saša Z.; Telečki, Mirjana; Matić, Jovana J.; Đilas, Sandra M.; Abramović, Biljana F.

(Matica srpska, Novi Sad, 2011)

TY  - JOUR
AU  - Jajić, Igor
AU  - Jevtić, Radivoje
AU  - Jurić, Verica B.
AU  - Krstović, Saša Z.
AU  - Telečki, Mirjana
AU  - Matić, Jovana J.
AU  - Đilas, Sandra M.
AU  - Abramović, Biljana F.
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/1017
AB  - Fusarium head blight (FHB) is present in all growing regions of small grains and causes significant losses in yield and grain quality. In our environmental conditions, dominant species is Fusarium graminearum Group 2. During 2009/10 there was a significant Fusarium infestation on wheat, barley and triticale. The aim of this study was to examine the contents of deoxynivalenol (DON) in cereal samples taken after 2009/10 harvest season. We analyzed 22 NS varieties of small grains from Rimski Šančevi, including 16 varieties of winter wheat, one facultative wheat variety, four varieties of winter barley and one variety of triticale. Analytical methods based on clean-up by solid-phase extraction (SPE) columns and detection by liquid chromatography were used. Fifteen out the 22 analyzed samples were positive for the presence of DON at a mean level of 0.537 mg/kg. The highest concentration was 1.952 mg/kg. These findings were in correlation with percentage of the Fusarium damaged kernels.
AB  - Patogeni iz roda Fusarium su prisutni u svim rejonima gajenja strnih žita i nanose značajne gubitke u prinosu i kvalitetu zrna. Fuzariozu klasa pšenice prouzrokuje veći broj vrsta iz roda Fusarium. U našim uslovima gajenja dominantna je vrsta Fusarium graminearum Grupa 2. U toku 2009/10. godine došlo je do značajne pojave fuzarioza na pšenici, ječmu i tritikaleu. Cilj rada je bio da se ispita sadržaj DON-a na uzorcima strnih žita uzetih posle žetve iz uslova prirodne zaraze. Analizirane su 22 novosadske sorte strnih žita iz lokaliteta Rimski Šančevi, od čega: 16 sorti ozime pšenice, jedna fakultativna sorta pšenice, četiri sorte ozimog ječma i jedna sorta tritikalea. Uzorci su uzeti sa parcela različitih površina u zavisnosti od značaja i rasprostranjenosti gajene sorte. Analitičko određivanje je zasnovano na prečišćavanju sirovog ekstrakta analiziranih uzoraka pomoću tzv. Mucosep kolona, a zatim je sadržaj DON-a kvantitativno određen tečnom hromatografijom. Od 22 analizirana uzorka strnih žita čak 15 (68,2%) je bilo pozitivno na prisustvo DON-a. Još veći procenat zaraženosti DON-om je utvrđen kada je u pitanju samo pšenica (82,4%). Prosečan sadržaj DON-a je iznosio 0,537 mg/kg a najveća koncentracija je utvrđena u uzorku tritikalea i iznosila je visokih 1,952 mg/kg. Od svih uzoraka koji su bili pozitivni na prisustvo ovog mikotoksina, 2 su prevazilazila koncentracije koje su propisane od strane Evropske komisije. Sve ovo ukazuje na visoku zaraženost strnih žita sa naših polja iz žetve 2010. Procenat fuzarioznih zrna kod 16 ispitivanih sorti pšenice kretao se od 1 do 11,5%, a gubici u masi 1000 zrna od 1,2 do 5,7%. Između jačine zaraze u polju i sadržaja mikotoksina DON ustanovljena je potpuna pozitivna korelacija kod pojedinih sorti. Fakultativna sorta pšenice Nataša je imala visoku koncentraciju DON od 1,572 mg/kg, pri stepenu zaraze u polju od 33,3% zaraženih klasova po 1 m2. Sorta Zvezdana imala je najniži procenat zaraze u polju od 1% i kod nje nije determinisano prisustvo mikotoksina DON.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Presence of deoxynivalenol in small-grain samples from 2009/10 harvest season
T1  - Prisustvo deoksinivalenola u uzorcima strnih žita u žetvenoj 2009/10. godini
EP  - 24
IS  - 120
SP  - 19
DO  - 10.2298/ZMSPN1120019J
ER  - 
@article{
author = "Jajić, Igor and Jevtić, Radivoje and Jurić, Verica B. and Krstović, Saša Z. and Telečki, Mirjana and Matić, Jovana J. and Đilas, Sandra M. and Abramović, Biljana F.",
year = "2011",
abstract = "Fusarium head blight (FHB) is present in all growing regions of small grains and causes significant losses in yield and grain quality. In our environmental conditions, dominant species is Fusarium graminearum Group 2. During 2009/10 there was a significant Fusarium infestation on wheat, barley and triticale. The aim of this study was to examine the contents of deoxynivalenol (DON) in cereal samples taken after 2009/10 harvest season. We analyzed 22 NS varieties of small grains from Rimski Šančevi, including 16 varieties of winter wheat, one facultative wheat variety, four varieties of winter barley and one variety of triticale. Analytical methods based on clean-up by solid-phase extraction (SPE) columns and detection by liquid chromatography were used. Fifteen out the 22 analyzed samples were positive for the presence of DON at a mean level of 0.537 mg/kg. The highest concentration was 1.952 mg/kg. These findings were in correlation with percentage of the Fusarium damaged kernels., Patogeni iz roda Fusarium su prisutni u svim rejonima gajenja strnih žita i nanose značajne gubitke u prinosu i kvalitetu zrna. Fuzariozu klasa pšenice prouzrokuje veći broj vrsta iz roda Fusarium. U našim uslovima gajenja dominantna je vrsta Fusarium graminearum Grupa 2. U toku 2009/10. godine došlo je do značajne pojave fuzarioza na pšenici, ječmu i tritikaleu. Cilj rada je bio da se ispita sadržaj DON-a na uzorcima strnih žita uzetih posle žetve iz uslova prirodne zaraze. Analizirane su 22 novosadske sorte strnih žita iz lokaliteta Rimski Šančevi, od čega: 16 sorti ozime pšenice, jedna fakultativna sorta pšenice, četiri sorte ozimog ječma i jedna sorta tritikalea. Uzorci su uzeti sa parcela različitih površina u zavisnosti od značaja i rasprostranjenosti gajene sorte. Analitičko određivanje je zasnovano na prečišćavanju sirovog ekstrakta analiziranih uzoraka pomoću tzv. Mucosep kolona, a zatim je sadržaj DON-a kvantitativno određen tečnom hromatografijom. Od 22 analizirana uzorka strnih žita čak 15 (68,2%) je bilo pozitivno na prisustvo DON-a. Još veći procenat zaraženosti DON-om je utvrđen kada je u pitanju samo pšenica (82,4%). Prosečan sadržaj DON-a je iznosio 0,537 mg/kg a najveća koncentracija je utvrđena u uzorku tritikalea i iznosila je visokih 1,952 mg/kg. Od svih uzoraka koji su bili pozitivni na prisustvo ovog mikotoksina, 2 su prevazilazila koncentracije koje su propisane od strane Evropske komisije. Sve ovo ukazuje na visoku zaraženost strnih žita sa naših polja iz žetve 2010. Procenat fuzarioznih zrna kod 16 ispitivanih sorti pšenice kretao se od 1 do 11,5%, a gubici u masi 1000 zrna od 1,2 do 5,7%. Između jačine zaraze u polju i sadržaja mikotoksina DON ustanovljena je potpuna pozitivna korelacija kod pojedinih sorti. Fakultativna sorta pšenice Nataša je imala visoku koncentraciju DON od 1,572 mg/kg, pri stepenu zaraze u polju od 33,3% zaraženih klasova po 1 m2. Sorta Zvezdana imala je najniži procenat zaraze u polju od 1% i kod nje nije determinisano prisustvo mikotoksina DON.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Presence of deoxynivalenol in small-grain samples from 2009/10 harvest season, Prisustvo deoksinivalenola u uzorcima strnih žita u žetvenoj 2009/10. godini",
pages = "24-19",
number = "120",
doi = "10.2298/ZMSPN1120019J"
}
Jajić, I., Jevtić, R., Jurić, V. B., Krstović, S. Z., Telečki, M., Matić, J. J., Đilas, S. M.,& Abramović, B. F.. (2011). Presence of deoxynivalenol in small-grain samples from 2009/10 harvest season. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(120), 19-24.
https://doi.org/10.2298/ZMSPN1120019J
Jajić I, Jevtić R, Jurić VB, Krstović SZ, Telečki M, Matić JJ, Đilas SM, Abramović BF. Presence of deoxynivalenol in small-grain samples from 2009/10 harvest season. in Zbornik Matice srpske za prirodne nauke. 2011;(120):19-24.
doi:10.2298/ZMSPN1120019J .
Jajić, Igor, Jevtić, Radivoje, Jurić, Verica B., Krstović, Saša Z., Telečki, Mirjana, Matić, Jovana J., Đilas, Sandra M., Abramović, Biljana F., "Presence of deoxynivalenol in small-grain samples from 2009/10 harvest season" in Zbornik Matice srpske za prirodne nauke, no. 120 (2011):19-24,
https://doi.org/10.2298/ZMSPN1120019J . .
4