TY  - JOUR
AU  - Kumar, Vivek
AU  - Sharma, Hemant
AU  - Saini, Lalita
AU  - Tyagi, Archasvi
AU  - Jain, Pooja
AU  - Singh, Yogita
AU  - Balyan, Priyanka
AU  - Kumar, Sachin
AU  - Jan, Sofora
AU  - Mir, Reyazul Rouf
AU  - Đalović, Ivica
AU  - Singh, Krishna Pal
AU  - Kumar, Upendra
AU  - Malik, Vijai
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3203
AB  - The core particle represents the catalytic portions of the 26S proteasomal complex. The genes encoding a- and b-subunits play a crucial role in protecting plants against various environmental stresses by controlling the quality of newly produced proteins. The 20S proteasome gene family has already been reported in model plants such as Arabidopsis and rice; however, they have not been studied in oilseed crops such as rapeseed (Brassica napus L.). In the present study, we identified 20S proteasome genes for a- (PA) and b-subunits (PB) in B. napus through systematically performed gene structure analysis, chromosomal location, conserved motif, phylogenetic relationship, and expression patterns. A total of 82 genes, comprising 35 BnPA and 47 BnPB of the 20S proteasome, were revealed in the B. napus genome. These genes were distributed on all 20 chromosomes of B. napus and most of these genes were duplicated on homoeologous chromosomes. The BnPA (a1-7) and BnPB (b1-7) genes were phylogenetically placed into seven clades. The pattern of expression of all the BnPA and BnPB genes was also studied using RNA-seq datasets under biotic and abiotic stress conditions. Out of 82 BnPA/PB genes, three exhibited high expression under abiotic stresses, whereas two genes were overexpressed in response to biotic stresses at both the seedling and flowering stages. Moreover, an additional eighteen genes were expressed under normal conditions. Overall, the current findings developed our understanding of the organization of the 20S proteasome genes in B. napus and provided specific BnPA/PB genes for further functional research in response to abiotic and biotic stresses.
PB  - Frontiers Media S.A.
T2  - Frontiers in Plant Science
T1  - Phylogenomic analysis of 20S proteasome gene family reveals stress-responsive patterns in rapeseed (Brassica napus L.)
SP  - 1037206
VL  - 13
DO  - 10.3389/fpls.2022.1037206
ER  - 

@article{
author = "Kumar, Vivek and Sharma, Hemant and Saini, Lalita and Tyagi, Archasvi and Jain, Pooja and Singh, Yogita and Balyan, Priyanka and Kumar, Sachin and Jan, Sofora and Mir, Reyazul Rouf and Đalović, Ivica and Singh, Krishna Pal and Kumar, Upendra and Malik, Vijai",
year = "2022",
abstract = "The core particle represents the catalytic portions of the 26S proteasomal complex. The genes encoding a- and b-subunits play a crucial role in protecting plants against various environmental stresses by controlling the quality of newly produced proteins. The 20S proteasome gene family has already been reported in model plants such as Arabidopsis and rice; however, they have not been studied in oilseed crops such as rapeseed (Brassica napus L.). In the present study, we identified 20S proteasome genes for a- (PA) and b-subunits (PB) in B. napus through systematically performed gene structure analysis, chromosomal location, conserved motif, phylogenetic relationship, and expression patterns. A total of 82 genes, comprising 35 BnPA and 47 BnPB of the 20S proteasome, were revealed in the B. napus genome. These genes were distributed on all 20 chromosomes of B. napus and most of these genes were duplicated on homoeologous chromosomes. The BnPA (a1-7) and BnPB (b1-7) genes were phylogenetically placed into seven clades. The pattern of expression of all the BnPA and BnPB genes was also studied using RNA-seq datasets under biotic and abiotic stress conditions. Out of 82 BnPA/PB genes, three exhibited high expression under abiotic stresses, whereas two genes were overexpressed in response to biotic stresses at both the seedling and flowering stages. Moreover, an additional eighteen genes were expressed under normal conditions. Overall, the current findings developed our understanding of the organization of the 20S proteasome genes in B. napus and provided specific BnPA/PB genes for further functional research in response to abiotic and biotic stresses.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Plant Science",
title = "Phylogenomic analysis of 20S proteasome gene family reveals stress-responsive patterns in rapeseed (Brassica napus L.)",
pages = "1037206",
volume = "13",
doi = "10.3389/fpls.2022.1037206"
}

Kumar, V., Sharma, H., Saini, L., Tyagi, A., Jain, P., Singh, Y., Balyan, P., Kumar, S., Jan, S., Mir, R. R., Đalović, I., Singh, K. P., Kumar, U.,& Malik, V.. (2022). Phylogenomic analysis of 20S proteasome gene family reveals stress-responsive patterns in rapeseed (Brassica napus L.). in Frontiers in Plant Science
Frontiers Media S.A.., 13, 1037206.
https://doi.org/10.3389/fpls.2022.1037206

Kumar V, Sharma H, Saini L, Tyagi A, Jain P, Singh Y, Balyan P, Kumar S, Jan S, Mir RR, Đalović I, Singh KP, Kumar U, Malik V. Phylogenomic analysis of 20S proteasome gene family reveals stress-responsive patterns in rapeseed (Brassica napus L.). in Frontiers in Plant Science. 2022;13:1037206.
doi:10.3389/fpls.2022.1037206 .

Kumar, Vivek, Sharma, Hemant, Saini, Lalita, Tyagi, Archasvi, Jain, Pooja, Singh, Yogita, Balyan, Priyanka, Kumar, Sachin, Jan, Sofora, Mir, Reyazul Rouf, Đalović, Ivica, Singh, Krishna Pal, Kumar, Upendra, Malik, Vijai, "Phylogenomic analysis of 20S proteasome gene family reveals stress-responsive patterns in rapeseed (Brassica napus L.)" in Frontiers in Plant Science, 13 (2022):1037206,
https://doi.org/10.3389/fpls.2022.1037206 . .