Pešić, Brankica

Link to this page

http://fiver.ifvcns.rs/APP/faces/author.xhtml?author_id=5ca9d1f3-cdc7-4467-9fa9-2d56955e6192&item_offset=0&project_offset=0&sort_by=dc.date.issued
Authority KeyName Variants
5ca9d1f3-cdc7-4467-9fa9-2d56955e6192
  • Pešić, Brankica (1)
Projects

Author's Bibliography

Specific detection of Waitea circinata var. zeae using conventional and real-time PCR

Vojvodić, Mira; Lazić, Dejan; Pešić, Brankica; Mitrović, Petar; Vico, Ivana; Bulajić, Aleksandra

(Springer Nature, 2024) 

TY  - JOUR
AU  - Vojvodić, Mira
AU  - Lazić, Dejan
AU  - Pešić, Brankica
AU  - Mitrović, Petar
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2024
UR  - http://fiver.ifvcns.rs/handle/123456789/4557
AB  - Waitea circinata var. zeae, a pathogen with a relatively narrow host range, has recently been detected in cabbage and oilseed rape in Europe and worldwide. In this study, we developed specific conventional and real-time PCR protocols for direct detection of W. circinata var. zeae from mycelium and diseased plant tissue. The newly developed primer pair zeaefor1/zeaerew1, used in PCR protocols, specifically amplified only target isolates of W. circinata var. zeae when tested against isolates of 11 different binucleate and multinucleate anastomosis groups of Rhizoctonia spp. including AG-A, AG-G, AG-F, AG-U, AG-2-1, AG-2-2, AG-3, AG-4 HGI, AG-4 HGII, AG-4 HGIII, and AG-6 and common soil-borne pathogens. Total of nine previously published primer pairs designed for the detection of various Rhizoctonia spp. were also tested and did not amplify target isolates of W. circinata var. zeae. The detection limit of conventional and real-time PCR protocols was 10–2 and 10–5 (with starting concentration 9.5 ng/µl), respectively, and both methods are the first available tools for direct detection and identification of W. circinata var. zeae from mycelium and diseased oilseed rape seedlings. Both conventional and SYBR-Green-based real-time PCR protocols are cost-effective and provide a solid basis for further investigations of W. circinata var. zeae, particularly in relation to distribution, host range, and epidemiology.
PB  - Springer Nature
T2  - Journal of Plant Diseases and Protection
T1  - Specific detection of Waitea circinata var. zeae using conventional and real-time PCR
DO  - 10.1007/s41348-024-00926-w
ER  - 
@article{
author = "Vojvodić, Mira and Lazić, Dejan and Pešić, Brankica and Mitrović, Petar and Vico, Ivana and Bulajić, Aleksandra",
year = "2024",
abstract = "Waitea circinata var. zeae, a pathogen with a relatively narrow host range, has recently been detected in cabbage and oilseed rape in Europe and worldwide. In this study, we developed specific conventional and real-time PCR protocols for direct detection of W. circinata var. zeae from mycelium and diseased plant tissue. The newly developed primer pair zeaefor1/zeaerew1, used in PCR protocols, specifically amplified only target isolates of W. circinata var. zeae when tested against isolates of 11 different binucleate and multinucleate anastomosis groups of Rhizoctonia spp. including AG-A, AG-G, AG-F, AG-U, AG-2-1, AG-2-2, AG-3, AG-4 HGI, AG-4 HGII, AG-4 HGIII, and AG-6 and common soil-borne pathogens. Total of nine previously published primer pairs designed for the detection of various Rhizoctonia spp. were also tested and did not amplify target isolates of W. circinata var. zeae. The detection limit of conventional and real-time PCR protocols was 10–2 and 10–5 (with starting concentration 9.5 ng/µl), respectively, and both methods are the first available tools for direct detection and identification of W. circinata var. zeae from mycelium and diseased oilseed rape seedlings. Both conventional and SYBR-Green-based real-time PCR protocols are cost-effective and provide a solid basis for further investigations of W. circinata var. zeae, particularly in relation to distribution, host range, and epidemiology.",
publisher = "Springer Nature",
journal = "Journal of Plant Diseases and Protection",
title = "Specific detection of Waitea circinata var. zeae using conventional and real-time PCR",
doi = "10.1007/s41348-024-00926-w"
}
Vojvodić, M., Lazić, D., Pešić, B., Mitrović, P., Vico, I.,& Bulajić, A.. (2024). Specific detection of Waitea circinata var. zeae using conventional and real-time PCR. in Journal of Plant Diseases and Protection
Springer Nature..
https://doi.org/10.1007/s41348-024-00926-w
Vojvodić M, Lazić D, Pešić B, Mitrović P, Vico I, Bulajić A. Specific detection of Waitea circinata var. zeae using conventional and real-time PCR. in Journal of Plant Diseases and Protection. 2024;.
doi:10.1007/s41348-024-00926-w .
Vojvodić, Mira, Lazić, Dejan, Pešić, Brankica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, "Specific detection of Waitea circinata var. zeae using conventional and real-time PCR" in Journal of Plant Diseases and Protection (2024),
https://doi.org/10.1007/s41348-024-00926-w . .
3