TY  - JOUR
AU  - Loc, Marta
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Ivanović, Žarko
AU  - Budakov, Dragana
AU  - Grahovac, Jovana
AU  - Vlajkov, Vanja
AU  - Pajčin, Ivana
AU  - Grahovac, Mila
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2679
AB  - Soft rot and blackleg are common diseases of potato (Solanum tuberosum) in Serbia. Opportunistic pectinolytic plant pathogens in the genus Pectobacterium cause soft rot and wilt diseases by cell wall degradation. They cause major economic losses in potato crops worldwide and are among the top 10 plant pathogenic bacteria (Mansfield et al. 2012). Potato plants (‘VR808’) with symptoms of wilting, slow growth, stem blackening, and tuber softening were collected from a commercial field in Zobnatica, Serbia, in July 2019 and analyzed. All symptoms occurred in the same field, and ~5% of plants were symptomatic. Isolation was performed from 10 randomly chosen symptomatic plants and tubers. Plant tissue was surface disinfected, and 1-cm sections from the margins of lesions were macerated in sterile distilled water (SDW) for 25 min and streaked on nutrient agar. After 48 h of incubation at 26C, predominant shiny, cream-colored, round colonies were obtained from all samples. Three representative isolates (MMZKVR1, MMZCVR2, and MMZKVR3) from independent samples were selected randomly for biochemical and pathogenicity tests. Isolates were gram-negative, nonfluorescent facultative anaerobes exhibiting pectinolytic activity on potato tuber slices and hypersensitive response on tobacco leaves. They expressed catalase activity but did not express oxidase or acid phosphatase activity or produce indole.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia
IS  - 5
SP  - 1513
VL  - 106
DO  - 10.1094/PDIS-06-21-1199-PDN
ER  - 

@article{
author = "Loc, Marta and Milošević, Dragana and Ignjatov, Maja and Ivanović, Žarko and Budakov, Dragana and Grahovac, Jovana and Vlajkov, Vanja and Pajčin, Ivana and Grahovac, Mila",
year = "2022",
abstract = "Soft rot and blackleg are common diseases of potato (Solanum tuberosum) in Serbia. Opportunistic pectinolytic plant pathogens in the genus Pectobacterium cause soft rot and wilt diseases by cell wall degradation. They cause major economic losses in potato crops worldwide and are among the top 10 plant pathogenic bacteria (Mansfield et al. 2012). Potato plants (‘VR808’) with symptoms of wilting, slow growth, stem blackening, and tuber softening were collected from a commercial field in Zobnatica, Serbia, in July 2019 and analyzed. All symptoms occurred in the same field, and ~5% of plants were symptomatic. Isolation was performed from 10 randomly chosen symptomatic plants and tubers. Plant tissue was surface disinfected, and 1-cm sections from the margins of lesions were macerated in sterile distilled water (SDW) for 25 min and streaked on nutrient agar. After 48 h of incubation at 26C, predominant shiny, cream-colored, round colonies were obtained from all samples. Three representative isolates (MMZKVR1, MMZCVR2, and MMZKVR3) from independent samples were selected randomly for biochemical and pathogenicity tests. Isolates were gram-negative, nonfluorescent facultative anaerobes exhibiting pectinolytic activity on potato tuber slices and hypersensitive response on tobacco leaves. They expressed catalase activity but did not express oxidase or acid phosphatase activity or produce indole.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia",
number = "5",
pages = "1513",
volume = "106",
doi = "10.1094/PDIS-06-21-1199-PDN"
}

Loc, M., Milošević, D., Ignjatov, M., Ivanović, Ž., Budakov, D., Grahovac, J., Vlajkov, V., Pajčin, I.,& Grahovac, M.. (2022). First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia. in Plant Disease
American Phytopathological Society., 106(5), 1513.
https://doi.org/10.1094/PDIS-06-21-1199-PDN

Loc M, Milošević D, Ignjatov M, Ivanović Ž, Budakov D, Grahovac J, Vlajkov V, Pajčin I, Grahovac M. First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia. in Plant Disease. 2022;106(5):1513.
doi:10.1094/PDIS-06-21-1199-PDN .

Loc, Marta, Milošević, Dragana, Ignjatov, Maja, Ivanović, Žarko, Budakov, Dragana, Grahovac, Jovana, Vlajkov, Vanja, Pajčin, Ivana, Grahovac, Mila, "First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia" in Plant Disease, 106, no. 5 (2022):1513,
https://doi.org/10.1094/PDIS-06-21-1199-PDN . .

TY  - JOUR
AU  - Pajčin, Ivana
AU  - Vlajkov, Vanja
AU  - Cvetković, D.D.
AU  - Ignjatov, Maja
AU  - Grahovac, Mila
AU  - Vučurović, D. G.
AU  - Grahovac, Jovana
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2065
AB  - Xanthomonas euvesicatoria is a worldwide causer of pepper bacterial spot, a bacterial plant disease responsible for massive losses of fresh pepper fruits. Considering the current problems in management of bacterial plant diseases, biological control using antagonistic microbial strains with high potential for plant pathogens suppression emerges as a possible solution. The aim of this study was to select suitable antagonists for suppression of X. euvesicatoria among the bacteria, yeast and fungi from the genera Pseudomonas, Lactobacillus, Saccharomyces and Trichoderma, based on in vitro antimicrobial activity testing using the diffusion disc method. The results of this study have revealed that cultivation broth samples of the antagonists Lactobacillus MK3 and Trichoderma reseii QM 9414, as well as supernatant samples of the antagonist Pseudomonas aeruginosa I128, have showed significant potential to be applied in biological control of X. euvesicatoria. Further research would be required to formulate suitable cultivation medium and optimize bioprocess conditions for production of the proposed pepper bacterial spot biocontrol agents.
PB  - Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad
T2  - Acta Periodica Technologica
T1  - Selection of antagonists for biocontrol of Xanthomonas euvesicatoria
EP  - 189
IS  - 51
SP  - 181
DO  - 10.2298/APT2051181P
ER  - 

@article{
author = "Pajčin, Ivana and Vlajkov, Vanja and Cvetković, D.D. and Ignjatov, Maja and Grahovac, Mila and Vučurović, D. G. and Grahovac, Jovana",
year = "2020",
abstract = "Xanthomonas euvesicatoria is a worldwide causer of pepper bacterial spot, a bacterial plant disease responsible for massive losses of fresh pepper fruits. Considering the current problems in management of bacterial plant diseases, biological control using antagonistic microbial strains with high potential for plant pathogens suppression emerges as a possible solution. The aim of this study was to select suitable antagonists for suppression of X. euvesicatoria among the bacteria, yeast and fungi from the genera Pseudomonas, Lactobacillus, Saccharomyces and Trichoderma, based on in vitro antimicrobial activity testing using the diffusion disc method. The results of this study have revealed that cultivation broth samples of the antagonists Lactobacillus MK3 and Trichoderma reseii QM 9414, as well as supernatant samples of the antagonist Pseudomonas aeruginosa I128, have showed significant potential to be applied in biological control of X. euvesicatoria. Further research would be required to formulate suitable cultivation medium and optimize bioprocess conditions for production of the proposed pepper bacterial spot biocontrol agents.",
publisher = "Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad",
journal = "Acta Periodica Technologica",
title = "Selection of antagonists for biocontrol of Xanthomonas euvesicatoria",
pages = "189-181",
number = "51",
doi = "10.2298/APT2051181P"
}

Pajčin, I., Vlajkov, V., Cvetković, D.D., Ignjatov, M., Grahovac, M., Vučurović, D. G.,& Grahovac, J.. (2020). Selection of antagonists for biocontrol of Xanthomonas euvesicatoria. in Acta Periodica Technologica
Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad.(51), 181-189.
https://doi.org/10.2298/APT2051181P

Pajčin I, Vlajkov V, Cvetković D, Ignjatov M, Grahovac M, Vučurović DG, Grahovac J. Selection of antagonists for biocontrol of Xanthomonas euvesicatoria. in Acta Periodica Technologica. 2020;(51):181-189.
doi:10.2298/APT2051181P .

Pajčin, Ivana, Vlajkov, Vanja, Cvetković, D.D., Ignjatov, Maja, Grahovac, Mila, Vučurović, D. G., Grahovac, Jovana, "Selection of antagonists for biocontrol of Xanthomonas euvesicatoria" in Acta Periodica Technologica, no. 51 (2020):181-189,
https://doi.org/10.2298/APT2051181P . .

TY  - JOUR
AU  - Pajčin, Ivana
AU  - Vlajkov, Vanja
AU  - Rončević, Zorana
AU  - Dodić, Jelena
AU  - Grahovac, Mila
AU  - Ignjatov, Maja
AU  - Grahovac, Jovana
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/1988
AB  - Bacterial pathogen Xanthomonas euvesicatoria, principal causer of bacterial spot, represents a significant problem in agricultural practice due to high yield losses in the production of pepper and tomato. The development of resistance to copper pesticides has shifted research, in the field of its suppression, towards biopesticides. In this study, several Bacillus strains were tested against Xanthomonas euvesicatoria strains, isolated from pepper leaves with symptoms of bacterial spot, to select a sufficiently effective antagonist. When it comes to the testing of cultivation broth, containing biomass of tested antagonists, the best results were achieved using isolate Bacillus sp3. On the other hand, when biomass-free supernatants, containing produced antimicrobial compounds, were tested, Bacillus sp1 and Bacillus sp2 have shown the highest antimicrobial activity. The results of this study represent a basis for further development of bioprocess solutions for the production of biopesticides based on Bacillus spp. biomass or antimicrobial compounds, showing high efficiency in suppression of pepper bacterial spot.
AB  - Bakterijski patogen Xanthomonas euvesicatoria predstavlja glavnog uzročnika bakteriozne pegavosti, stoga predstavlja značajan problem u poljoprivrednoj praksi usled izazivanja velikih gubitaka prinosa u proizvodnji paprike i paradajza. Uobičajena sredstva za suzbijanje i kontrolu ovog fitopatogena predstavljaju preparati na bazi bakra. Međutim, razvoj rezistentnosti prema pesticidima na bazi bakra doveo je do zaokreta u polju suzbijanja ovih patogena ka primeni biopesticida, odnosno bioloških kontrolnih agensa. Bakterije roda Bacillus i njihovi metaboliti sa izraženom antimikrobnom aktivnošću protiv ciljanih fitopatogena predstavljaju najperspektivnije aktivne komponente biokontrolnih preparata za zaštitu bilja. U ovom istraživanju nekoliko sojeva roda Bacillus ispitano je sa ciljem odabira antagonista dovoljno efikasnih u suzbijanju sojeva Xanthomonas euvesicatoria, koji su izolovani sa listova paprike sa simptomima bakteriozne pegavosti. Prilikom testiranja antimikrobne aktivnosti uzoraka kultivacionih tečnosti, koji sadrže i biomasu testiranih antagonista, najbolji rezultati u suzbijanju testiranih fitopatogena su postignuti primenom izolata Bacillus sp3. Sa druge strane, prilikom testiranja antimikrobne aktivnosti supernatanata oslobođenih biomase antagonista, koji sadrže samo produkovana antimikrobna jedinjenja, izolati Bacillus sp1 i Bacillus sp2 su pokazali najveću antimikrobnu aktivnost protiv fitopatogena Xanthomonas euvesicatoria. Rezultati ovog istraživanja predstavljaju osnovu za dalji razvoj bioprocesnih rešenja za proizvodnju biopesticida na bazi biomase ili antimikrobnih jedinjenja koja proizvode antagonisti roda Bacillus, a koji pokazuju visoku efikasnost u suzbijanju bakteriozne pegavosti paprike.
PB  - Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad
T2  - Journal on Processing & Energy in Agriculture
T1  - In vitro potential of Bacillus spp. Antagonists for suppression of Xanthomonas euvesicatoria phytopathogens
T1  - In vitro potencijal antagonista Bacillus spp. za suzbijanje fitopatogena Xanthomonas euvesicatoria
EP  - 76
IS  - 2
SP  - 72
VL  - 24
DO  - 10.5937/jpea24-27076
ER  - 

@article{
author = "Pajčin, Ivana and Vlajkov, Vanja and Rončević, Zorana and Dodić, Jelena and Grahovac, Mila and Ignjatov, Maja and Grahovac, Jovana",
year = "2020",
abstract = "Bacterial pathogen Xanthomonas euvesicatoria, principal causer of bacterial spot, represents a significant problem in agricultural practice due to high yield losses in the production of pepper and tomato. The development of resistance to copper pesticides has shifted research, in the field of its suppression, towards biopesticides. In this study, several Bacillus strains were tested against Xanthomonas euvesicatoria strains, isolated from pepper leaves with symptoms of bacterial spot, to select a sufficiently effective antagonist. When it comes to the testing of cultivation broth, containing biomass of tested antagonists, the best results were achieved using isolate Bacillus sp3. On the other hand, when biomass-free supernatants, containing produced antimicrobial compounds, were tested, Bacillus sp1 and Bacillus sp2 have shown the highest antimicrobial activity. The results of this study represent a basis for further development of bioprocess solutions for the production of biopesticides based on Bacillus spp. biomass or antimicrobial compounds, showing high efficiency in suppression of pepper bacterial spot., Bakterijski patogen Xanthomonas euvesicatoria predstavlja glavnog uzročnika bakteriozne pegavosti, stoga predstavlja značajan problem u poljoprivrednoj praksi usled izazivanja velikih gubitaka prinosa u proizvodnji paprike i paradajza. Uobičajena sredstva za suzbijanje i kontrolu ovog fitopatogena predstavljaju preparati na bazi bakra. Međutim, razvoj rezistentnosti prema pesticidima na bazi bakra doveo je do zaokreta u polju suzbijanja ovih patogena ka primeni biopesticida, odnosno bioloških kontrolnih agensa. Bakterije roda Bacillus i njihovi metaboliti sa izraženom antimikrobnom aktivnošću protiv ciljanih fitopatogena predstavljaju najperspektivnije aktivne komponente biokontrolnih preparata za zaštitu bilja. U ovom istraživanju nekoliko sojeva roda Bacillus ispitano je sa ciljem odabira antagonista dovoljno efikasnih u suzbijanju sojeva Xanthomonas euvesicatoria, koji su izolovani sa listova paprike sa simptomima bakteriozne pegavosti. Prilikom testiranja antimikrobne aktivnosti uzoraka kultivacionih tečnosti, koji sadrže i biomasu testiranih antagonista, najbolji rezultati u suzbijanju testiranih fitopatogena su postignuti primenom izolata Bacillus sp3. Sa druge strane, prilikom testiranja antimikrobne aktivnosti supernatanata oslobođenih biomase antagonista, koji sadrže samo produkovana antimikrobna jedinjenja, izolati Bacillus sp1 i Bacillus sp2 su pokazali najveću antimikrobnu aktivnost protiv fitopatogena Xanthomonas euvesicatoria. Rezultati ovog istraživanja predstavljaju osnovu za dalji razvoj bioprocesnih rešenja za proizvodnju biopesticida na bazi biomase ili antimikrobnih jedinjenja koja proizvode antagonisti roda Bacillus, a koji pokazuju visoku efikasnost u suzbijanju bakteriozne pegavosti paprike.",
publisher = "Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad",
journal = "Journal on Processing & Energy in Agriculture",
title = "In vitro potential of Bacillus spp. Antagonists for suppression of Xanthomonas euvesicatoria phytopathogens, In vitro potencijal antagonista Bacillus spp. za suzbijanje fitopatogena Xanthomonas euvesicatoria",
pages = "76-72",
number = "2",
volume = "24",
doi = "10.5937/jpea24-27076"
}

Pajčin, I., Vlajkov, V., Rončević, Z., Dodić, J., Grahovac, M., Ignjatov, M.,& Grahovac, J.. (2020). In vitro potential of Bacillus spp. Antagonists for suppression of Xanthomonas euvesicatoria phytopathogens. in Journal on Processing & Energy in Agriculture
Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad., 24(2), 72-76.
https://doi.org/10.5937/jpea24-27076

Pajčin I, Vlajkov V, Rončević Z, Dodić J, Grahovac M, Ignjatov M, Grahovac J. In vitro potential of Bacillus spp. Antagonists for suppression of Xanthomonas euvesicatoria phytopathogens. in Journal on Processing & Energy in Agriculture. 2020;24(2):72-76.
doi:10.5937/jpea24-27076 .

Pajčin, Ivana, Vlajkov, Vanja, Rončević, Zorana, Dodić, Jelena, Grahovac, Mila, Ignjatov, Maja, Grahovac, Jovana, "In vitro potential of Bacillus spp. Antagonists for suppression of Xanthomonas euvesicatoria phytopathogens" in Journal on Processing & Energy in Agriculture, 24, no. 2 (2020):72-76,
https://doi.org/10.5937/jpea24-27076 . .