@mastersthesis{
author = "Popov, Olivera",
year = "2022",
abstract = "Od velikog značaja su proučavanja vrsta koje nanose
velike ekonomske gubitke na najznačajnijim
poljoprivrednim kulturama kao što je kukuruz, vrsta
koja prouzrokuje najveće ekonomske gubitke na poljima
kukuruza je dijapauzirajući moljac Ostrinia nubilalis.
Dijapauza je hipometabolička vrsta mirovanja koju
karakterišu smanjena stopa oksidativnog
metabolizma, sinteza zaštitnih molekula,
akumulacija energetskih rezervi i ekspresija
specifičnih gena. Dokazano je da dijapauzirajuće
vrste imaju znatno veću otpornost na insekticidne
tretmane, te je od velikog značaja sprovođenje
istraživanja koja za cilj imaju testiranje biološkog
odgovora na pesticide koji se nalaze komercijalno u
upotrebi Insekticidni tretman može dovesti do
oksidativnog stresa, u okviru čega se produkuju
enzimi antioksidativne zaštite kao što su
superoksid dismutaza (SOD) i katalaza (CAT).
Ispitivan je uticaj dva folijarna i dva semena
insekticida, od kojih je jedan biološki, dok su
ostali sintetički. U okviru eksperimenta urađena je
provera da li insekticidni tretman dovodi do pojave
oksidativnog stresa kod preživelih larvi O.
nubilalis, koji će rezultovati povećanom ekspresijom
gena za SOD i CAT. Ukupna RNK je izolovana iz
homogenata celih dijapauzirajućih gusenica petog
instara koje su preživele insekticidni tretman.
Nakon sinteze komplementarne DNK, sprovedena je
kvantitativna PCR analiza, te je pomoću dobijenih
Cq vrednosti izračunata relativna ekspresija gena
koristeći aktin kao referentni gen. Pokazano je da
postoji statistički značajno povećanje relativne
ekspresije gena za SOD i CAT u odnosu na kontrolnu
grupu prilikom primene sintetičkog insekticida
Fobos. Biološki insekticid Lepinoks i sintetički
insekticid Sonido doveli su do statistički značajno
povećane ekspresije gena za CAT, dok je kod relativne
ekspresije gena za SOD došlo do blažeg smanjenja.
Prilikom primene insekticida Semafor na seme nije
došlo do statistički značajnih promena u relativnoj
ekspresiji ni jednog od ispitivanih gena u odnosu na
kontrolnu grupu. Rezultati ovog istraživanja su
pionirske prirode, jer prema našim saznanjima ne
postoje podaci o efektu pesticida na ekspresiju ovde
ispitivanih gena., Research on economically significant pests of agricultural
crops, such as corn, is of great importance, тhe most
damaging insect species, when it comes to corn crops, is
the diapausing moth Ostrinia nubilalis. Diapause is a
hypometabolic type of rest characterized by a reduced rate
of oxidative metabolism, synthesis of protective
molecules, accumulation of energy reserves and
expression of specific genes. It has been proven that
diapausing species have a significantly higher resistance to
insecticide treatments, so conducting research aimed at
testing the biological response is of great importance.
Insecticide treatment can cause oxidative stress, in
response to which antioxidant protection enzymes, such as
superoxide dismutase (SOD) and catalase (CAT), are
produced. The effect of two foliar and two seed
insecticides was tested. One of the four insecticides is a
bioinsecticide, while the other three are synthetic. As part
of the experiment, it was examined whether the insecticide
treatment leads to the occurrence of oxidative stress in the
larvae of O. nubilalis, which will result in increased
expression of sod and cat genes. Total RNA was isolated
from homogenates of whole-body diapausing 5th instar
larvae that survived the insecticide treatment. After the
synthesis of complementary DNA, quantitative PCR
analysis was carried out, and relative gene expression was
calculated using the Cq values obtained using actin as the
reference gene. It was shown that there is a statistically
significant increase in the relative expression of both sod
and cat genes in comparison to the control group when
applying the synthetic insecticide Phobos. The biological
insecticide Lepinox and the synthetic insecticide Sonido
led to a statistically significant increase in the expression
of cat gene, while the relative expression of the gene for
SOD was mildly decreased. When applying the insecticide
Semaphore to seeds, there were no statistically significant
changes in the relative expression of any of the tested
genes compared to the control group. The results of this
research are of a pioneer nature, as to our knowledge there
is no data on pesticide treatment affects the expression of
genes analysed in this study.",
title = "Dejstvo odabranih pesticida na relativnu ekspresiju gena za katalazu i superoksid dismutazu kukuruznog plamenca Ostrinia nubilalis (Hbn.), The impact of selected pesticides on relative expression of superoxide dismutase and catalase, two antioxidant enzyme genes, in the European corn borer Ostrinia nubilalis (Hbn.)",
url = "https://hdl.handle.net/21.15107/rcub_fiver_4434"
}
