TY  - JOUR
AU  - Puvača, Nikola
AU  - Halfawi, Rabea Ai Bashir M.
AU  - Ćosić, Milivoje
AU  - Prodanović, Radivoj
AU  - Soleša, Dragan
AU  - Vladisavljević, Radovan
AU  - Lekić, Snežana
AU  - Aćimović, Milica
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2681
AB  - Recently, there has been an increasing demand for natural, healthy, and safe products without residual antibiotics for human consumption, particularly bee products. Beekeepers have been struggling with this problem many years, having in mind often occurrence of American foulbrood (AFB), which is one of the most severe honey bee brood diseases, and in the past have been successfully eradicated with heavy usage of antibiotics. Such controlled, or mostly uncontrolled usage of antibiotics in fighting against American foulbrood lead to a residual quantity of antibiotic in honey. To overcome this problem, this research aimed to investigate the influence of single essential oil (Lavandula angustifolia) in protecting bees against AFB compared to the antibiotic oxytetracycline. Totally three treatments were formed artificially infected with P. larvae spore suspension, at concentration 2×109 spore/ml. The course of the disease was regularly monitored. Treatment one (T1) did not receive antibiotic therapy. Treatment two (T2) was given lavender essential oil at a concentration of 0.1% of sugar syrup. The treatment was applied for 30 days, at 48h intervals. Treatment three (T3) received antibiotics in the sugar syrup at a concentration of 0.1%, respectively. Clinical and laboratory examinations were performed on days 10, 20, 40 and 60, respectively. Besides, L. angustifolia essential oil rich in Ethanol, 2- (2-ethoxyethoxy) - (13.05%), linalool (10.71%), α-Terpinyl acetate (10.93%) and linalool acetate (9.60%), showed its positive effects against antibiotics in combat of American foulbrood, further research with a specifically designed qualitative and quantitative mixture of essential oils are more than necessary because single essential oil is not enough and didn’t show expected results.
PB  - Athens : Hellenic Veterinary Medical Society
T2  - Journal of the Hellenic Veterinary Medical Society
T1  - The influence of single lavender essential oil in honey bee prevention of American foulbrood and overall welfare
EP  - 3782
IS  - 1
SP  - 3773
VL  - 73
DO  - 10.12681/jhvms.25747
ER  - 

@article{
author = "Puvača, Nikola and Halfawi, Rabea Ai Bashir M. and Ćosić, Milivoje and Prodanović, Radivoj and Soleša, Dragan and Vladisavljević, Radovan and Lekić, Snežana and Aćimović, Milica",
year = "2022",
abstract = "Recently, there has been an increasing demand for natural, healthy, and safe products without residual antibiotics for human consumption, particularly bee products. Beekeepers have been struggling with this problem many years, having in mind often occurrence of American foulbrood (AFB), which is one of the most severe honey bee brood diseases, and in the past have been successfully eradicated with heavy usage of antibiotics. Such controlled, or mostly uncontrolled usage of antibiotics in fighting against American foulbrood lead to a residual quantity of antibiotic in honey. To overcome this problem, this research aimed to investigate the influence of single essential oil (Lavandula angustifolia) in protecting bees against AFB compared to the antibiotic oxytetracycline. Totally three treatments were formed artificially infected with P. larvae spore suspension, at concentration 2×109 spore/ml. The course of the disease was regularly monitored. Treatment one (T1) did not receive antibiotic therapy. Treatment two (T2) was given lavender essential oil at a concentration of 0.1% of sugar syrup. The treatment was applied for 30 days, at 48h intervals. Treatment three (T3) received antibiotics in the sugar syrup at a concentration of 0.1%, respectively. Clinical and laboratory examinations were performed on days 10, 20, 40 and 60, respectively. Besides, L. angustifolia essential oil rich in Ethanol, 2- (2-ethoxyethoxy) - (13.05%), linalool (10.71%), α-Terpinyl acetate (10.93%) and linalool acetate (9.60%), showed its positive effects against antibiotics in combat of American foulbrood, further research with a specifically designed qualitative and quantitative mixture of essential oils are more than necessary because single essential oil is not enough and didn’t show expected results.",
publisher = "Athens : Hellenic Veterinary Medical Society",
journal = "Journal of the Hellenic Veterinary Medical Society",
title = "The influence of single lavender essential oil in honey bee prevention of American foulbrood and overall welfare",
pages = "3782-3773",
number = "1",
volume = "73",
doi = "10.12681/jhvms.25747"
}

Puvača, N., Halfawi, R. A. B. M., Ćosić, M., Prodanović, R., Soleša, D., Vladisavljević, R., Lekić, S.,& Aćimović, M.. (2022). The influence of single lavender essential oil in honey bee prevention of American foulbrood and overall welfare. in Journal of the Hellenic Veterinary Medical Society
Athens : Hellenic Veterinary Medical Society., 73(1), 3773-3782.
https://doi.org/10.12681/jhvms.25747

Puvača N, Halfawi RABM, Ćosić M, Prodanović R, Soleša D, Vladisavljević R, Lekić S, Aćimović M. The influence of single lavender essential oil in honey bee prevention of American foulbrood and overall welfare. in Journal of the Hellenic Veterinary Medical Society. 2022;73(1):3773-3782.
doi:10.12681/jhvms.25747 .

Puvača, Nikola, Halfawi, Rabea Ai Bashir M., Ćosić, Milivoje, Prodanović, Radivoj, Soleša, Dragan, Vladisavljević, Radovan, Lekić, Snežana, Aćimović, Milica, "The influence of single lavender essential oil in honey bee prevention of American foulbrood and overall welfare" in Journal of the Hellenic Veterinary Medical Society, 73, no. 1 (2022):3773-3782,
https://doi.org/10.12681/jhvms.25747 . .

TY  - JOUR
AU  - Stojanović, Tijana
AU  - Vuković, Gorica
AU  - Petrović, Aleksandra
AU  - Konstantinović, Bojan
AU  - Puvača, Nikola
AU  - Marinković, Dušan
AU  - Gvozdenac, Sonja
AU  - Bursić, Vojislava
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2828
AB  - The interest in tropane alkaloids (TA) as food contaminants is increasing. A sensitive and selective LC-MS/MS method was applied for the analysis of corn puff samples from the Serbian market. Only atropine was quantified in 22% of the samples. In case of scopolamine, although not quantified, it was detected in 22% of the samples. Whether the acute reference dose (ARfD) could be exceeded was checked on a case-by-case basis for the individual products under assessment. Due to their low body weight and relatively high snack consumption, preschool children were at the highest risk of TA exposure. Assuming that the average consumption is 50 g of corn puffs per day, the sample with the highest concentration of TAs (2.05 µg/kg, 1.58 μg/kg of atropine) could contribute with 32.0% to the ARfD, of which 24.7% owing to atropine. If the same amount of corn puffs is consumed by older age classes, corresponding exposure contributions to the ARfD would progressively decline, down to 8.4% for adult population. The study revealed no health risk from TAs exposure through the consumption of the corn puffs in Serbian population.
AB  - Interesovanje za tropanske alkaloide kao kontaminante hrane je u porastu. Za analizu kukuruznog flipsa sa tržišta Srbije primenjena je osetljiva i selektivna LC-MS/MS metoda. Atropin je kvantifikovan u 22% uzoraka. U slučaju skopolamina, koji nije kvantifikovan, detektovano je u 22% uzoraka. Da li može doći do prekoračenja akutne referentne doze provereno je za svaki pojedinačni proizvod koji je uvršten u procenu. Mala telesna masa i relativno velike konzumirane količine snek-proizvoda ističu predškolsku decu kao grupu u najvećem riziku od izloženosti tropanskim alkaloidima. Pod pretpostavkom o konzumiranju 50 grama kukuruznog flipsa dnevno, uzorak s naj­ većom koncentracijom tropanskih alkaloida (2,05 µg/kg, 1,58 mg/kg atropina) može doprineti sa 32,0% od akutne referentne doze, od čega 24,7% odgovara atropinu. Ukoliko istu količinu kukuruznog flipsa konzumiraju starije uzrasne grupe, doprinos sledstvene izloženosti tropanskim alkaloidima bi progresivno opadao do 8,4% za odraslu populaciju. Studija nije ukazala na zdravstveni rizik usled izloženosti tropanskim alkaloidima putem konzumiranja kukuru­ znog flipsa za populaciju u Srbiji.
PB  - Novi Sad : Matica srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Determination of tropane alkaloids in corn puffs by the LC-MS/MS
T1  - Određivanje tropanskih alkaloida u kukuruznom flipsu primenom LC-MS/MS
EP  - 80
SP  - 69
VL  - 141
DO  - 10.2298/ZMSPN2141069S
ER  - 

@article{
author = "Stojanović, Tijana and Vuković, Gorica and Petrović, Aleksandra and Konstantinović, Bojan and Puvača, Nikola and Marinković, Dušan and Gvozdenac, Sonja and Bursić, Vojislava",
year = "2021",
abstract = "The interest in tropane alkaloids (TA) as food contaminants is increasing. A sensitive and selective LC-MS/MS method was applied for the analysis of corn puff samples from the Serbian market. Only atropine was quantified in 22% of the samples. In case of scopolamine, although not quantified, it was detected in 22% of the samples. Whether the acute reference dose (ARfD) could be exceeded was checked on a case-by-case basis for the individual products under assessment. Due to their low body weight and relatively high snack consumption, preschool children were at the highest risk of TA exposure. Assuming that the average consumption is 50 g of corn puffs per day, the sample with the highest concentration of TAs (2.05 µg/kg, 1.58 μg/kg of atropine) could contribute with 32.0% to the ARfD, of which 24.7% owing to atropine. If the same amount of corn puffs is consumed by older age classes, corresponding exposure contributions to the ARfD would progressively decline, down to 8.4% for adult population. The study revealed no health risk from TAs exposure through the consumption of the corn puffs in Serbian population., Interesovanje za tropanske alkaloide kao kontaminante hrane je u porastu. Za analizu kukuruznog flipsa sa tržišta Srbije primenjena je osetljiva i selektivna LC-MS/MS metoda. Atropin je kvantifikovan u 22% uzoraka. U slučaju skopolamina, koji nije kvantifikovan, detektovano je u 22% uzoraka. Da li može doći do prekoračenja akutne referentne doze provereno je za svaki pojedinačni proizvod koji je uvršten u procenu. Mala telesna masa i relativno velike konzumirane količine snek-proizvoda ističu predškolsku decu kao grupu u najvećem riziku od izloženosti tropanskim alkaloidima. Pod pretpostavkom o konzumiranju 50 grama kukuruznog flipsa dnevno, uzorak s naj­ većom koncentracijom tropanskih alkaloida (2,05 µg/kg, 1,58 mg/kg atropina) može doprineti sa 32,0% od akutne referentne doze, od čega 24,7% odgovara atropinu. Ukoliko istu količinu kukuruznog flipsa konzumiraju starije uzrasne grupe, doprinos sledstvene izloženosti tropanskim alkaloidima bi progresivno opadao do 8,4% za odraslu populaciju. Studija nije ukazala na zdravstveni rizik usled izloženosti tropanskim alkaloidima putem konzumiranja kukuru­ znog flipsa za populaciju u Srbiji.",
publisher = "Novi Sad : Matica srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Determination of tropane alkaloids in corn puffs by the LC-MS/MS, Određivanje tropanskih alkaloida u kukuruznom flipsu primenom LC-MS/MS",
pages = "80-69",
volume = "141",
doi = "10.2298/ZMSPN2141069S"
}

Stojanović, T., Vuković, G., Petrović, A., Konstantinović, B., Puvača, N., Marinković, D., Gvozdenac, S.,& Bursić, V.. (2021). Determination of tropane alkaloids in corn puffs by the LC-MS/MS. in Zbornik Matice srpske za prirodne nauke
Novi Sad : Matica srpska., 141, 69-80.
https://doi.org/10.2298/ZMSPN2141069S

Stojanović T, Vuković G, Petrović A, Konstantinović B, Puvača N, Marinković D, Gvozdenac S, Bursić V. Determination of tropane alkaloids in corn puffs by the LC-MS/MS. in Zbornik Matice srpske za prirodne nauke. 2021;141:69-80.
doi:10.2298/ZMSPN2141069S .

Stojanović, Tijana, Vuković, Gorica, Petrović, Aleksandra, Konstantinović, Bojan, Puvača, Nikola, Marinković, Dušan, Gvozdenac, Sonja, Bursić, Vojislava, "Determination of tropane alkaloids in corn puffs by the LC-MS/MS" in Zbornik Matice srpske za prirodne nauke, 141 (2021):69-80,
https://doi.org/10.2298/ZMSPN2141069S . .

TY  - JOUR
AU  - Aćimović, Milica
AU  - Čabarkapa, Ivana
AU  - Cvetković, Mirjana
AU  - Stanković Jeremić, Jovana
AU  - Kiprovski, Biljana
AU  - Gvozdenac, Sonja
AU  - Puvača, Nikola
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/2219
AB  - The genus Cymbopogon (Greek words 'kymbe' meaning boat and 'pogon' meaning beard), commonly known as lemongrass, belongs to the Poaceae family. It comprises a large number species, but only two have economic importance as cultivated plants: C. citratus and C. flexuosus. However, C. citratus is more resistant to drought and low temperatures and because of that can be cultivated over large areas therefore it is commercially more important. C. citratus leaves are widely used as a lemon flavor ingredient in herbal teas, prepared either by decoction or infusion, or in finished herbal products such as capsules, tablets and creams. Even though essential oils are known for being used for fragrance and as an important ingredient in Asian cuisine, they are also used in other industries (pharmaceutical and cosmetic) due to their bioactive compounds that show various therapeutic effects. The chemical composition of C. citratus essential oil varies widely depending on genetic diversity, habitat and agronomic treatment of the culture, as well as on the part of the plant, maturity stage and extraction method. However, the essential oil of C. citratus mainly consists of the citral, which is a mixture of two isomeric acyclic monoterpene aldehides: geranial (trans-citral) and neral (cis-citral). C. citratus possesses good antibacterial activity, it could be used as alternative treatment for enteric fever, to cure infectious diseases related to the respiratory system, as well as for oral hygiene, it helps by removing bacteria from the oral cavity and prevents teeth and gum diseases such as peridontitis, plaque and gingivitis. Furthermore, C. citratus showed high contents of total phenolic and total flavonoids, as well as high free radical scavenging capacity with potential as an antioxidant. C. citratus shows good antiinflamatory, anti-diabetic, hypolipidemic, renoprotective and cardioprotective, as well as anticancer activities. Apart from this, C. citratus possesses vasorelaxant, sedative and antitusive potential. Furthermore, the compound citral is used in perfume industry as well as for cleaning wounds and treatment of skin diseases in forms of gels, or functional paper microencapsulated with essential oil, which can be used for hand hygiene.
PB  - Novi Sad : University Business Academy in Novi Sad, Faculty of Economics and Engineering Management
T2  - Journal of Agronomy, Technology and Engineering Management (JATEM)
T1  - Cymbopogon citratus (DC.) Staph: chemical composition, antimicrobial and antioxidant activities, use in medicinal and cosmetic purpose
EP  - 360
IS  - 6
SP  - 344
VL  - 2
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2219
ER  - 

@article{
author = "Aćimović, Milica and Čabarkapa, Ivana and Cvetković, Mirjana and Stanković Jeremić, Jovana and Kiprovski, Biljana and Gvozdenac, Sonja and Puvača, Nikola",
year = "2019",
abstract = "The genus Cymbopogon (Greek words 'kymbe' meaning boat and 'pogon' meaning beard), commonly known as lemongrass, belongs to the Poaceae family. It comprises a large number species, but only two have economic importance as cultivated plants: C. citratus and C. flexuosus. However, C. citratus is more resistant to drought and low temperatures and because of that can be cultivated over large areas therefore it is commercially more important. C. citratus leaves are widely used as a lemon flavor ingredient in herbal teas, prepared either by decoction or infusion, or in finished herbal products such as capsules, tablets and creams. Even though essential oils are known for being used for fragrance and as an important ingredient in Asian cuisine, they are also used in other industries (pharmaceutical and cosmetic) due to their bioactive compounds that show various therapeutic effects. The chemical composition of C. citratus essential oil varies widely depending on genetic diversity, habitat and agronomic treatment of the culture, as well as on the part of the plant, maturity stage and extraction method. However, the essential oil of C. citratus mainly consists of the citral, which is a mixture of two isomeric acyclic monoterpene aldehides: geranial (trans-citral) and neral (cis-citral). C. citratus possesses good antibacterial activity, it could be used as alternative treatment for enteric fever, to cure infectious diseases related to the respiratory system, as well as for oral hygiene, it helps by removing bacteria from the oral cavity and prevents teeth and gum diseases such as peridontitis, plaque and gingivitis. Furthermore, C. citratus showed high contents of total phenolic and total flavonoids, as well as high free radical scavenging capacity with potential as an antioxidant. C. citratus shows good antiinflamatory, anti-diabetic, hypolipidemic, renoprotective and cardioprotective, as well as anticancer activities. Apart from this, C. citratus possesses vasorelaxant, sedative and antitusive potential. Furthermore, the compound citral is used in perfume industry as well as for cleaning wounds and treatment of skin diseases in forms of gels, or functional paper microencapsulated with essential oil, which can be used for hand hygiene.",
publisher = "Novi Sad : University Business Academy in Novi Sad, Faculty of Economics and Engineering Management",
journal = "Journal of Agronomy, Technology and Engineering Management (JATEM)",
title = "Cymbopogon citratus (DC.) Staph: chemical composition, antimicrobial and antioxidant activities, use in medicinal and cosmetic purpose",
pages = "360-344",
number = "6",
volume = "2",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2219"
}

Aćimović, M., Čabarkapa, I., Cvetković, M., Stanković Jeremić, J., Kiprovski, B., Gvozdenac, S.,& Puvača, N.. (2019). Cymbopogon citratus (DC.) Staph: chemical composition, antimicrobial and antioxidant activities, use in medicinal and cosmetic purpose. in Journal of Agronomy, Technology and Engineering Management (JATEM)
Novi Sad : University Business Academy in Novi Sad, Faculty of Economics and Engineering Management., 2(6), 344-360.
https://hdl.handle.net/21.15107/rcub_fiver_2219

Aćimović M, Čabarkapa I, Cvetković M, Stanković Jeremić J, Kiprovski B, Gvozdenac S, Puvača N. Cymbopogon citratus (DC.) Staph: chemical composition, antimicrobial and antioxidant activities, use in medicinal and cosmetic purpose. in Journal of Agronomy, Technology and Engineering Management (JATEM). 2019;2(6):344-360.
https://hdl.handle.net/21.15107/rcub_fiver_2219 .

Aćimović, Milica, Čabarkapa, Ivana, Cvetković, Mirjana, Stanković Jeremić, Jovana, Kiprovski, Biljana, Gvozdenac, Sonja, Puvača, Nikola, "Cymbopogon citratus (DC.) Staph: chemical composition, antimicrobial and antioxidant activities, use in medicinal and cosmetic purpose" in Journal of Agronomy, Technology and Engineering Management (JATEM), 2, no. 6 (2019):344-360,
https://hdl.handle.net/21.15107/rcub_fiver_2219 .