TY  - JOUR
AU  - Đalović, Ivica
AU  - Mitrović, Petar
AU  - Trivan, Goran
AU  - Jelušić, Aleksandra
AU  - Pezo, Lato
AU  - Janić Hajnal, Elizabet
AU  - Popović Milovanović, Tatjana
PY  - 2024
UR  - http://fiver.ifvcns.rs/handle/123456789/4548
AB  - Infections with phytoplasma present one of the most significant biotic stresses influencing plant health, growth, and production. The phytoplasma ‘Candidatus Phytoplasma solani’ infects a variety of plant species. This pathogen impacts the physiological and morphological characteristics of plants causing stunting, yellowing, leaf curling, and other symptoms that can lead to significant economic losses. The aim of this study was to determine biochemical changes in peony (Paeonia tenuifolia L.), mint (Mentha × piperita L.), and dill (Anethum graveolens L.) induced by ‘Ca. Phytoplasma solani’ in Serbia as well as to predict the impact of the biotic stress using artificial neural network (ANN) modeling. The phylogenetic position of the Serbian ‘Ca. Phytoplasma solani’ strains originated from the tested hosts using 16S rRNA (peony and carrot strains) and plsC (mint and dill strains) sequences indicated by their genetic homogeneity despite the host of origin. Biochemical parameters significantly differed in asymptomatic and symptomatic plants, except for total anthocyanidins contents in dill and the capacity of peony and mint extracts to neutralize superoxide anions and hydroxyl radicals, respectively. Principal Component Analysis (PCA) showed a correlation between different chemical parameters and revealed a clear separation among the samples. Based on the ANN performance, the optimal number of hidden neurons for the calculation of TS, RG, PAL, LP, NBT, •OH, TP, TT, Tflav, Tpro, Tant, DPPH, and Car was nine (using MLP 8-9-13), as it produced high r2 values (1.000 during the training period) and low SOS values. Developing an effective early warning system for the detection of plant diseases in different plant species is critical for improving crop yield and quality.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach
IS  - 5
SP  - 426
VL  - 10
DO  - 10.3390/horticulturae10050426
ER  - 

@article{
author = "Đalović, Ivica and Mitrović, Petar and Trivan, Goran and Jelušić, Aleksandra and Pezo, Lato and Janić Hajnal, Elizabet and Popović Milovanović, Tatjana",
year = "2024",
abstract = "Infections with phytoplasma present one of the most significant biotic stresses influencing plant health, growth, and production. The phytoplasma ‘Candidatus Phytoplasma solani’ infects a variety of plant species. This pathogen impacts the physiological and morphological characteristics of plants causing stunting, yellowing, leaf curling, and other symptoms that can lead to significant economic losses. The aim of this study was to determine biochemical changes in peony (Paeonia tenuifolia L.), mint (Mentha × piperita L.), and dill (Anethum graveolens L.) induced by ‘Ca. Phytoplasma solani’ in Serbia as well as to predict the impact of the biotic stress using artificial neural network (ANN) modeling. The phylogenetic position of the Serbian ‘Ca. Phytoplasma solani’ strains originated from the tested hosts using 16S rRNA (peony and carrot strains) and plsC (mint and dill strains) sequences indicated by their genetic homogeneity despite the host of origin. Biochemical parameters significantly differed in asymptomatic and symptomatic plants, except for total anthocyanidins contents in dill and the capacity of peony and mint extracts to neutralize superoxide anions and hydroxyl radicals, respectively. Principal Component Analysis (PCA) showed a correlation between different chemical parameters and revealed a clear separation among the samples. Based on the ANN performance, the optimal number of hidden neurons for the calculation of TS, RG, PAL, LP, NBT, •OH, TP, TT, Tflav, Tpro, Tant, DPPH, and Car was nine (using MLP 8-9-13), as it produced high r2 values (1.000 during the training period) and low SOS values. Developing an effective early warning system for the detection of plant diseases in different plant species is critical for improving crop yield and quality.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach",
number = "5",
pages = "426",
volume = "10",
doi = "10.3390/horticulturae10050426"
}

Đalović, I., Mitrović, P., Trivan, G., Jelušić, A., Pezo, L., Janić Hajnal, E.,& Popović Milovanović, T.. (2024). The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach. in Horticulturae
Basel : MDPI., 10(5), 426.
https://doi.org/10.3390/horticulturae10050426

Đalović I, Mitrović P, Trivan G, Jelušić A, Pezo L, Janić Hajnal E, Popović Milovanović T. The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach. in Horticulturae. 2024;10(5):426.
doi:10.3390/horticulturae10050426 .

Đalović, Ivica, Mitrović, Petar, Trivan, Goran, Jelušić, Aleksandra, Pezo, Lato, Janić Hajnal, Elizabet, Popović Milovanović, Tatjana, "The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach" in Horticulturae, 10, no. 5 (2024):426,
https://doi.org/10.3390/horticulturae10050426 . .

TY  - CONF
AU  - Popović Milovanović, Tatjana
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Trkulja, Vojislav
AU  - Milovanović, Predrag
AU  - Zečević, Katarina
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/4178
AB  - Veliki ekonomski značaj u gajenju brojnih biljnih vrsta, a posebno korenasto-krtolastih, imaju bakterije iz rodova Pectobacterium i Dickeya, prvobitno opisane kao rod Erwinia. Ove bakterije prouzrokuju bolest tzv. vlažnu trulež. Štete nastaju usled propadanja zaraženih plodova, krtola i mesnatog korena. Iako ovi patogeni mogu da prouzrokuju velike štete tokom proizvodnje, ipak najveće nastaju tokom skladištenja. Primenom metode sekvenciranja DNK genoma, taksonomija rodova Pectobacterium i Dickeya je u prethodnoj deceniji značajno izmenjena,usled čega je i pripadnost pojedinih, već opisanih sojeva vrstama iz ovih rodova iznova determinisana. Takođe, ova metodologija doprinela je otkrivanju velikog broja novih vrsta bakterija ovih rodova. Tako, rod Pectobacterium do danas broji 20 vrsta, a rod Dickeya najmanje 10. S obzirom na učestale taksonomske promene, cilj ovog rada je bio da se izoluju i identifikuju prouzrokovači vlažne truleži paprike i mrkve kako bi se utvrdio genetički diverzitet novoizolovanih bakterijskih populacija.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova, 17. Simpozijum o zaštiti bilja, Zlatibor, 27-30. novembar 2023.
T1  - Proučavanje prouzrokovača vlažne truleži paprike i mrkve u Vojvodini
EP  - 48
SP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_fiver_4178
ER  - 

@conference{
author = "Popović Milovanović, Tatjana and Iličić, Renata and Jelušić, Aleksandra and Mitrović, Petar and Trkulja, Vojislav and Milovanović, Predrag and Zečević, Katarina",
year = "2023",
abstract = "Veliki ekonomski značaj u gajenju brojnih biljnih vrsta, a posebno korenasto-krtolastih, imaju bakterije iz rodova Pectobacterium i Dickeya, prvobitno opisane kao rod Erwinia. Ove bakterije prouzrokuju bolest tzv. vlažnu trulež. Štete nastaju usled propadanja zaraženih plodova, krtola i mesnatog korena. Iako ovi patogeni mogu da prouzrokuju velike štete tokom proizvodnje, ipak najveće nastaju tokom skladištenja. Primenom metode sekvenciranja DNK genoma, taksonomija rodova Pectobacterium i Dickeya je u prethodnoj deceniji značajno izmenjena,usled čega je i pripadnost pojedinih, već opisanih sojeva vrstama iz ovih rodova iznova determinisana. Takođe, ova metodologija doprinela je otkrivanju velikog broja novih vrsta bakterija ovih rodova. Tako, rod Pectobacterium do danas broji 20 vrsta, a rod Dickeya najmanje 10. S obzirom na učestale taksonomske promene, cilj ovog rada je bio da se izoluju i identifikuju prouzrokovači vlažne truleži paprike i mrkve kako bi se utvrdio genetički diverzitet novoizolovanih bakterijskih populacija.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova, 17. Simpozijum o zaštiti bilja, Zlatibor, 27-30. novembar 2023.",
title = "Proučavanje prouzrokovača vlažne truleži paprike i mrkve u Vojvodini",
pages = "48-48",
url = "https://hdl.handle.net/21.15107/rcub_fiver_4178"
}

Popović Milovanović, T., Iličić, R., Jelušić, A., Mitrović, P., Trkulja, V., Milovanović, P.,& Zečević, K.. (2023). Proučavanje prouzrokovača vlažne truleži paprike i mrkve u Vojvodini. in Zbornik rezimea radova, 17. Simpozijum o zaštiti bilja, Zlatibor, 27-30. novembar 2023.
Beograd : Društvo za zaštitu bilja Srbije., 48-48.
https://hdl.handle.net/21.15107/rcub_fiver_4178

Popović Milovanović T, Iličić R, Jelušić A, Mitrović P, Trkulja V, Milovanović P, Zečević K. Proučavanje prouzrokovača vlažne truleži paprike i mrkve u Vojvodini. in Zbornik rezimea radova, 17. Simpozijum o zaštiti bilja, Zlatibor, 27-30. novembar 2023.. 2023;:48-48.
https://hdl.handle.net/21.15107/rcub_fiver_4178 .

Popović Milovanović, Tatjana, Iličić, Renata, Jelušić, Aleksandra, Mitrović, Petar, Trkulja, Vojislav, Milovanović, Predrag, Zečević, Katarina, "Proučavanje prouzrokovača vlažne truleži paprike i mrkve u Vojvodini" in Zbornik rezimea radova, 17. Simpozijum o zaštiti bilja, Zlatibor, 27-30. novembar 2023. (2023):48-48,
https://hdl.handle.net/21.15107/rcub_fiver_4178 .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Popović Milovanović, Tatjana
AU  - Mitrović, Petar
AU  - Iličić, Renata
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/4179
AB  - Bakterija Xanthomonas campestris pv. campestris (Xcc) je sveprisutna kao prouzrokovač crne truleži biljaka iz familije kupusnjača, pre svega kupusa, a zatim i karfiola, brokolija, kelja, prokelja, kelerabe, raštana, ali i uljane repice, slačice, rotkvice, hrena, kres salate, određenih sorti model biljke Arabidopsis thaliana i nekih korovskih vrsta i ukrasnih biljaka. Negativnim uticajem na rast i razvoj domaćina, a time i veličinu i kvalitet prinosa, dovodi do značajnih ekonomskih gubitaka. Beležene štete izazvane ovim patogenom procenjuju se na više od 50% na kupusu, odnosno 50 – 70% na karfiolu. U Srbiji se Xcc nakon prve pojave na kelju 1960-ih, redovno javlja na svim gajenim kupusnjačama. S obzirom na obim i tradiciju gajenja različitih kupusnjača, ova bakterija je najznačajniji patogen kupusa. Dosadašnje genetičke analize prisutnih populacija Xcc poreklom sa kupusa u Srbiji nisu ukazale na postojanje diverziteta, bez obzira na sortu, godinu ili lokalitet sa kog su izolovane. Cilj ovog rada je da se izoluju i genetički okarakterišu novije populacije bakterije Xcc poreklom sa kupusa.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova, 17. Simpozijum o zaštiti bilja, Zlatibor, 27-30. novembar 2023.
T1  - Proučavanje populacija Xanthomonas campestris pv. campestris poreklom sa kupusa u Vojvodini
EP  - 47
SP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_fiver_4179
ER  - 

@conference{
author = "Jelušić, Aleksandra and Popović Milovanović, Tatjana and Mitrović, Petar and Iličić, Renata",
year = "2023",
abstract = "Bakterija Xanthomonas campestris pv. campestris (Xcc) je sveprisutna kao prouzrokovač crne truleži biljaka iz familije kupusnjača, pre svega kupusa, a zatim i karfiola, brokolija, kelja, prokelja, kelerabe, raštana, ali i uljane repice, slačice, rotkvice, hrena, kres salate, određenih sorti model biljke Arabidopsis thaliana i nekih korovskih vrsta i ukrasnih biljaka. Negativnim uticajem na rast i razvoj domaćina, a time i veličinu i kvalitet prinosa, dovodi do značajnih ekonomskih gubitaka. Beležene štete izazvane ovim patogenom procenjuju se na više od 50% na kupusu, odnosno 50 – 70% na karfiolu. U Srbiji se Xcc nakon prve pojave na kelju 1960-ih, redovno javlja na svim gajenim kupusnjačama. S obzirom na obim i tradiciju gajenja različitih kupusnjača, ova bakterija je najznačajniji patogen kupusa. Dosadašnje genetičke analize prisutnih populacija Xcc poreklom sa kupusa u Srbiji nisu ukazale na postojanje diverziteta, bez obzira na sortu, godinu ili lokalitet sa kog su izolovane. Cilj ovog rada je da se izoluju i genetički okarakterišu novije populacije bakterije Xcc poreklom sa kupusa.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova, 17. Simpozijum o zaštiti bilja, Zlatibor, 27-30. novembar 2023.",
title = "Proučavanje populacija Xanthomonas campestris pv. campestris poreklom sa kupusa u Vojvodini",
pages = "47-47",
url = "https://hdl.handle.net/21.15107/rcub_fiver_4179"
}

Jelušić, A., Popović Milovanović, T., Mitrović, P.,& Iličić, R.. (2023). Proučavanje populacija Xanthomonas campestris pv. campestris poreklom sa kupusa u Vojvodini. in Zbornik rezimea radova, 17. Simpozijum o zaštiti bilja, Zlatibor, 27-30. novembar 2023.
Beograd : Društvo za zaštitu bilja Srbije., 47-47.
https://hdl.handle.net/21.15107/rcub_fiver_4179

Jelušić A, Popović Milovanović T, Mitrović P, Iličić R. Proučavanje populacija Xanthomonas campestris pv. campestris poreklom sa kupusa u Vojvodini. in Zbornik rezimea radova, 17. Simpozijum o zaštiti bilja, Zlatibor, 27-30. novembar 2023.. 2023;:47-47.
https://hdl.handle.net/21.15107/rcub_fiver_4179 .

Jelušić, Aleksandra, Popović Milovanović, Tatjana, Mitrović, Petar, Iličić, Renata, "Proučavanje populacija Xanthomonas campestris pv. campestris poreklom sa kupusa u Vojvodini" in Zbornik rezimea radova, 17. Simpozijum o zaštiti bilja, Zlatibor, 27-30. novembar 2023. (2023):47-47,
https://hdl.handle.net/21.15107/rcub_fiver_4179 .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Iličić, Renata
AU  - Milovanović, Predrag
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3286
AB  - The aim of this work was to identify and characterize the pectolytic bacteria responsible for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo F1) grown in the Futog locality (Baˇcka, Vojvodina), known for the five-century-long tradition of cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were observed during August 2021, while the inner tissues were macerated, featuring cream to black discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP) medium were characterized for their phenotypic and genotypic features. The pathogenicity was confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating the presence of diverse Pectobacterium species even in combined infection in the same field. Among the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA, dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was found to be the most virulent species for cabbage, followed by P. versatile, while all three species had comparable virulence with respect to potato. The results obtained in this work provide a better understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.
PB  - Basel : MDPI
T2  - Microorganisms - Basel
T1  - Diversity of bacterial soft rot-causing Pectobacterium species affecting cabbage in Serbia
IS  - 2
SP  - 335
VL  - 11
DO  - 10.3390/microorganisms11020335
ER  - 

@article{
author = "Jelušić, Aleksandra and Mitrović, Petar and Marković, Sanja and Iličić, Renata and Milovanović, Predrag and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this work was to identify and characterize the pectolytic bacteria responsible for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo F1) grown in the Futog locality (Baˇcka, Vojvodina), known for the five-century-long tradition of cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were observed during August 2021, while the inner tissues were macerated, featuring cream to black discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP) medium were characterized for their phenotypic and genotypic features. The pathogenicity was confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating the presence of diverse Pectobacterium species even in combined infection in the same field. Among the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA, dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was found to be the most virulent species for cabbage, followed by P. versatile, while all three species had comparable virulence with respect to potato. The results obtained in this work provide a better understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.",
publisher = "Basel : MDPI",
journal = "Microorganisms - Basel",
title = "Diversity of bacterial soft rot-causing Pectobacterium species affecting cabbage in Serbia",
number = "2",
pages = "335",
volume = "11",
doi = "10.3390/microorganisms11020335"
}

Jelušić, A., Mitrović, P., Marković, S., Iličić, R., Milovanović, P., Stanković, S.,& Popović Milovanović, T.. (2023). Diversity of bacterial soft rot-causing Pectobacterium species affecting cabbage in Serbia. in Microorganisms - Basel
Basel : MDPI., 11(2), 335.
https://doi.org/10.3390/microorganisms11020335

Jelušić A, Mitrović P, Marković S, Iličić R, Milovanović P, Stanković S, Popović Milovanović T. Diversity of bacterial soft rot-causing Pectobacterium species affecting cabbage in Serbia. in Microorganisms - Basel. 2023;11(2):335.
doi:10.3390/microorganisms11020335 .

Jelušić, Aleksandra, Mitrović, Petar, Marković, Sanja, Iličić, Renata, Milovanović, Predrag, Stanković, Slaviša, Popović Milovanović, Tatjana, "Diversity of bacterial soft rot-causing Pectobacterium species affecting cabbage in Serbia" in Microorganisms - Basel, 11, no. 2 (2023):335,
https://doi.org/10.3390/microorganisms11020335 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Scortichini, Marco
AU  - Marković, Sanja
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3737
AB  - The aim of this study was to establish a link between genetic diversity and the geographic origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.
PB  - Basel : MDPI
T2  - Microorganisms - Basel
T1  - Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia
IS  - 8
SP  - 2122
VL  - 11
DO  - 10.3390/microorganisms11082122
ER  - 

@article{
author = "Jelušić, Aleksandra and Scortichini, Marco and Marković, Sanja and Mitrović, Petar and Iličić, Renata and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this study was to establish a link between genetic diversity and the geographic origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.",
publisher = "Basel : MDPI",
journal = "Microorganisms - Basel",
title = "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia",
number = "8",
pages = "2122",
volume = "11",
doi = "10.3390/microorganisms11082122"
}

Jelušić, A., Scortichini, M., Marković, S., Mitrović, P., Iličić, R., Stanković, S.,& Popović Milovanović, T.. (2023). Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms - Basel
Basel : MDPI., 11(8), 2122.
https://doi.org/10.3390/microorganisms11082122

Jelušić A, Scortichini M, Marković S, Mitrović P, Iličić R, Stanković S, Popović Milovanović T. Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms - Basel. 2023;11(8):2122.
doi:10.3390/microorganisms11082122 .

Jelušić, Aleksandra, Scortichini, Marco, Marković, Sanja, Mitrović, Petar, Iličić, Renata, Stanković, Slaviša, Popović Milovanović, Tatjana, "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia" in Microorganisms - Basel, 11, no. 8 (2023):2122,
https://doi.org/10.3390/microorganisms11082122 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Peeters, Kelly
AU  - Miklavčić Višnjevec, Ana
AU  - Tavzes, Črtomir
AU  - Stanković, Slaviša
AU  - Berić, Tanja
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/3750
AB  - Plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) was recently described as a pathogen of winter oilseed rape in Serbia. A metabarcoding approach was used to study bacterial community composition changes in the phyllosphere and rhizosphere of different winter oilseed rape types (lines, cultivars, and hybrids) naturally infected with Xcc. This study also aimed to examine the potential of indigenous Bacillus and Pseudomonas isolates to suppress Xcc. Infection with Xcc leads to depletion of bacterial diversity in the plants' phyllosphere and explicitly decreasing the abundance of genera such as Exiguobacterium, Massilia, and Pantoea with potentially beneficial properties. Strains identified as Bacillus velezensis X5-2, Bacillus megaterium X6-3, and Pseudomonas orientalis X2-1P obtained from oilseed rape phyllosphere were found to be effective against Xcc in vitro and in vivo when applied as a whole-culture and as a cell-free supernatant. The greenhouse in vivo tests on winter oilseed rape plants with three selected biocontrol strains lead to a disease reduction of 82.37% and 72.47% in preventive and curative treatments, respectively. Genetic screening showed potential for the biosynthesis of surfactin, kurstakin, bacillomycin D, and iturin in B. velezensis X5-2, as well as surfactin and kurstakin in B. megaterium X6-3. Gene phcA encoding phenazine-1-carboxylic acid was detected in P. orientalis X2-1P. The chemical composition of ethyl acetate and benzene extracts of three biocontrol strains obtained by GC–MS and HPLC-ESI-qTOF/MS analyses indicates numerous volatile organic compounds (alkenes, benzenes, carboxylic acids, indoles, pyrazines, etc.), lipopeptides, and/or antibiotics, for many of which antimicrobial potential is proven. We assume that this wide range of metabolites is responsible for the exhibited biocontrol activity against Xcc. These results recommend all three biocontrol strains for further studies for use as agents for biocontrol of bacterial blight-like disease caused by Xcc of oilseed rape.
PB  - Elsevier
T2  - Biological Control
T1  - Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates
SP  - 104695
VL  - 160
DO  - 10.1016/j.biocontrol.2021.104695
ER  - 

@article{
author = "Jelušić, Aleksandra and Popović, Tatjana and Dimkić, Ivica and Mitrović, Petar and Peeters, Kelly and Miklavčić Višnjevec, Ana and Tavzes, Črtomir and Stanković, Slaviša and Berić, Tanja",
year = "2021",
abstract = "Plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) was recently described as a pathogen of winter oilseed rape in Serbia. A metabarcoding approach was used to study bacterial community composition changes in the phyllosphere and rhizosphere of different winter oilseed rape types (lines, cultivars, and hybrids) naturally infected with Xcc. This study also aimed to examine the potential of indigenous Bacillus and Pseudomonas isolates to suppress Xcc. Infection with Xcc leads to depletion of bacterial diversity in the plants' phyllosphere and explicitly decreasing the abundance of genera such as Exiguobacterium, Massilia, and Pantoea with potentially beneficial properties. Strains identified as Bacillus velezensis X5-2, Bacillus megaterium X6-3, and Pseudomonas orientalis X2-1P obtained from oilseed rape phyllosphere were found to be effective against Xcc in vitro and in vivo when applied as a whole-culture and as a cell-free supernatant. The greenhouse in vivo tests on winter oilseed rape plants with three selected biocontrol strains lead to a disease reduction of 82.37% and 72.47% in preventive and curative treatments, respectively. Genetic screening showed potential for the biosynthesis of surfactin, kurstakin, bacillomycin D, and iturin in B. velezensis X5-2, as well as surfactin and kurstakin in B. megaterium X6-3. Gene phcA encoding phenazine-1-carboxylic acid was detected in P. orientalis X2-1P. The chemical composition of ethyl acetate and benzene extracts of three biocontrol strains obtained by GC–MS and HPLC-ESI-qTOF/MS analyses indicates numerous volatile organic compounds (alkenes, benzenes, carboxylic acids, indoles, pyrazines, etc.), lipopeptides, and/or antibiotics, for many of which antimicrobial potential is proven. We assume that this wide range of metabolites is responsible for the exhibited biocontrol activity against Xcc. These results recommend all three biocontrol strains for further studies for use as agents for biocontrol of bacterial blight-like disease caused by Xcc of oilseed rape.",
publisher = "Elsevier",
journal = "Biological Control",
title = "Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates",
pages = "104695",
volume = "160",
doi = "10.1016/j.biocontrol.2021.104695"
}

Jelušić, A., Popović, T., Dimkić, I., Mitrović, P., Peeters, K., Miklavčić Višnjevec, A., Tavzes, Č., Stanković, S.,& Berić, T.. (2021). Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates. in Biological Control
Elsevier., 160, 104695.
https://doi.org/10.1016/j.biocontrol.2021.104695

Jelušić A, Popović T, Dimkić I, Mitrović P, Peeters K, Miklavčić Višnjevec A, Tavzes Č, Stanković S, Berić T. Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates. in Biological Control. 2021;160:104695.
doi:10.1016/j.biocontrol.2021.104695 .

Jelušić, Aleksandra, Popović, Tatjana, Dimkić, Ivica, Mitrović, Petar, Peeters, Kelly, Miklavčić Višnjevec, Ana, Tavzes, Črtomir, Stanković, Slaviša, Berić, Tanja, "Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates" in Biological Control, 160 (2021):104695,
https://doi.org/10.1016/j.biocontrol.2021.104695 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Mitrović, Petar
AU  - Dimkić, Ivica
AU  - Stanković, Slaviša
AU  - Marjanović-Jeromela, Ana
AU  - Popović, Tatjana
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2097
AB  - Winter oilseed rape has recently been described as a new host for the phytopathogenic bacteriumXanthomonas campestrispv.campestris(Xcc) in Serbia. In order to provide information about the genetic structure of this bacterium's population and its pathogenic features on this crop, a collection of 65 winter oilseed rape Xcc isolates obtained over a 5-year period (2014-2018) from different Serbian localities was examined. The presence of intrapathovar diversity within the tested isolates was confirmed by repetitive element PCR (rep-PCR), revealing 14, 10, and 4 different profiles for BOX, ERIC, and REP primers, respectively. A multilocus sequence typing (MLST) approach separated the isolates into seven groups on a phylogenetic tree. The PubMLST non-redundant database was used to compare sequences of four housekeeping genes from isolates with 75 strains ofXanthomonasspp. to determine nucleotide changes caused by host-pathogen coevolution. Five allelic profile combinations (ST3, ST5, ST9, ST26, and ST47) were determined, with ST47 being the most frequent. Pathogenicity and host range tests showed higher virulence of the tested isolates on winter oilseed rape than on otherBrassica oleraceahosts (broccoli, cabbage, cauliflower, collard greens, kale, and kohlrabi). The most aggressive isolates were NCPPB4679, NCPPB4680, and Xc361, belonging to genotype ST26. Fifty oilseed rape lines, cultivars, and hybrids showed susceptibility to the three Xcc isolates tested (of high, low, and moderate virulence), indicating that there was no resistance to the bacterium. The results provide significant information about diversification of Xcc isolates and their evolution toward specialization for winter oilseed rape.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia
EP  - 49
IS  - 1
SP  - 35
VL  - 70
DO  - 10.1111/ppa.13273
ER  - 

@article{
author = "Jelušić, Aleksandra and Berić, Tanja and Mitrović, Petar and Dimkić, Ivica and Stanković, Slaviša and Marjanović-Jeromela, Ana and Popović, Tatjana",
year = "2021",
abstract = "Winter oilseed rape has recently been described as a new host for the phytopathogenic bacteriumXanthomonas campestrispv.campestris(Xcc) in Serbia. In order to provide information about the genetic structure of this bacterium's population and its pathogenic features on this crop, a collection of 65 winter oilseed rape Xcc isolates obtained over a 5-year period (2014-2018) from different Serbian localities was examined. The presence of intrapathovar diversity within the tested isolates was confirmed by repetitive element PCR (rep-PCR), revealing 14, 10, and 4 different profiles for BOX, ERIC, and REP primers, respectively. A multilocus sequence typing (MLST) approach separated the isolates into seven groups on a phylogenetic tree. The PubMLST non-redundant database was used to compare sequences of four housekeeping genes from isolates with 75 strains ofXanthomonasspp. to determine nucleotide changes caused by host-pathogen coevolution. Five allelic profile combinations (ST3, ST5, ST9, ST26, and ST47) were determined, with ST47 being the most frequent. Pathogenicity and host range tests showed higher virulence of the tested isolates on winter oilseed rape than on otherBrassica oleraceahosts (broccoli, cabbage, cauliflower, collard greens, kale, and kohlrabi). The most aggressive isolates were NCPPB4679, NCPPB4680, and Xc361, belonging to genotype ST26. Fifty oilseed rape lines, cultivars, and hybrids showed susceptibility to the three Xcc isolates tested (of high, low, and moderate virulence), indicating that there was no resistance to the bacterium. The results provide significant information about diversification of Xcc isolates and their evolution toward specialization for winter oilseed rape.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia",
pages = "49-35",
number = "1",
volume = "70",
doi = "10.1111/ppa.13273"
}

Jelušić, A., Berić, T., Mitrović, P., Dimkić, I., Stanković, S., Marjanović-Jeromela, A.,& Popović, T.. (2021). New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia. in Plant Pathology
Wiley, Hoboken., 70(1), 35-49.
https://doi.org/10.1111/ppa.13273

Jelušić A, Berić T, Mitrović P, Dimkić I, Stanković S, Marjanović-Jeromela A, Popović T. New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia. in Plant Pathology. 2021;70(1):35-49.
doi:10.1111/ppa.13273 .

Jelušić, Aleksandra, Berić, Tanja, Mitrović, Petar, Dimkić, Ivica, Stanković, Slaviša, Marjanović-Jeromela, Ana, Popović, Tatjana, "New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia" in Plant Pathology, 70, no. 1 (2021):35-49,
https://doi.org/10.1111/ppa.13273 . .

TY  - JOUR
AU  - Mitrović, Petar
AU  - Đalović, Ivica
AU  - Kiprovski, Biljana
AU  - Veljović-Jovanović, Sonja
AU  - Trkulja, Vojislav
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2078
AB  - The present study examined the effects of Candidatus Phytoplasma solani infection on antioxidative metabolism in leaves and roots of carrot (Daucus carota L.). Disease symptoms appeared at the end of June in the form of the chlorosis on some of the leaves, which became intensely red one week later, while the previously healthy leaves from the same branch becme chlorotic. A few days later, all leaves from the infected leaf branch were intensely red. Infected plants also had slower growth compared to the healthy ones with fewer leaf branches developed. The roots of infected plants were less developed, seared, or gummy with or without brown-colored root hair. The presence of the pathogen was detected by sequencing the 16S rRNA. National Center for Biotechnology Information (NCBI) BLAST analyses of the obtained sequence revealed 100% identity of tested strain with deposited Ca. Phytoplasma solani strains from various countries and hosts, all belonging to the "stolbur" group (16SrXII-A). Identity of 99.74% was found when the tested Serbian strain (MF503627) was compared with the reference stolbur strain STOL11 (AF248959). The oxidative damage of membranes in carrot cells was accompanied by a decrease in the content of photosynthetic pigments. Furthermore, for the determination of specific scavenging properties of the extracts, in vitro antioxidant assay was performed. In phytoplasma-infected carrot leaves, there was a greater reduction in the level of glutathione content (GSH); however; flavonoids and anthocyanidins seem to be responsible for the accompanied increased antioxidative capacity against hydroxyl radical and hydrogen peroxide.
PB  - Basel : MDPI
T2  - Plants-Basel
T1  - Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani
IS  - 2
VL  - 10
DO  - 10.3390/plants10020337
ER  - 

@article{
author = "Mitrović, Petar and Đalović, Ivica and Kiprovski, Biljana and Veljović-Jovanović, Sonja and Trkulja, Vojislav and Jelušić, Aleksandra and Popović, Tatjana",
year = "2021",
abstract = "The present study examined the effects of Candidatus Phytoplasma solani infection on antioxidative metabolism in leaves and roots of carrot (Daucus carota L.). Disease symptoms appeared at the end of June in the form of the chlorosis on some of the leaves, which became intensely red one week later, while the previously healthy leaves from the same branch becme chlorotic. A few days later, all leaves from the infected leaf branch were intensely red. Infected plants also had slower growth compared to the healthy ones with fewer leaf branches developed. The roots of infected plants were less developed, seared, or gummy with or without brown-colored root hair. The presence of the pathogen was detected by sequencing the 16S rRNA. National Center for Biotechnology Information (NCBI) BLAST analyses of the obtained sequence revealed 100% identity of tested strain with deposited Ca. Phytoplasma solani strains from various countries and hosts, all belonging to the "stolbur" group (16SrXII-A). Identity of 99.74% was found when the tested Serbian strain (MF503627) was compared with the reference stolbur strain STOL11 (AF248959). The oxidative damage of membranes in carrot cells was accompanied by a decrease in the content of photosynthetic pigments. Furthermore, for the determination of specific scavenging properties of the extracts, in vitro antioxidant assay was performed. In phytoplasma-infected carrot leaves, there was a greater reduction in the level of glutathione content (GSH); however; flavonoids and anthocyanidins seem to be responsible for the accompanied increased antioxidative capacity against hydroxyl radical and hydrogen peroxide.",
publisher = "Basel : MDPI",
journal = "Plants-Basel",
title = "Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani",
number = "2",
volume = "10",
doi = "10.3390/plants10020337"
}

Mitrović, P., Đalović, I., Kiprovski, B., Veljović-Jovanović, S., Trkulja, V., Jelušić, A.,& Popović, T.. (2021). Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani. in Plants-Basel
Basel : MDPI., 10(2).
https://doi.org/10.3390/plants10020337

Mitrović P, Đalović I, Kiprovski B, Veljović-Jovanović S, Trkulja V, Jelušić A, Popović T. Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani. in Plants-Basel. 2021;10(2).
doi:10.3390/plants10020337 .

Mitrović, Petar, Đalović, Ivica, Kiprovski, Biljana, Veljović-Jovanović, Sonja, Trkulja, Vojislav, Jelušić, Aleksandra, Popović, Tatjana, "Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani" in Plants-Basel, 10, no. 2 (2021),
https://doi.org/10.3390/plants10020337 . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Marković, Sanja
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/1989
AB  - Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease of cabbage (Brassica oleracea var. capitata L.), is one of the most important bacteria which affect proper cabbage growth, leading to head weight and quality losses and thereby drastically reducing its marketing value. The pathogen is genetically diverse, which is evident from the presence of eleven races worldwide and more than thirty combinations of allelic profiles. Therefore, this study aimed to determine the allelic profiles of Serbian cabbage Xcc strains obtained in 2014. The analysis was done on three selected Xcc strains whose DNA was first amplified using polymerase chain reaction (PCR) with four housekeeping genes - P-XdnaK, fyuA, gyrB, and rpoD, then sequenced, and the obtained sequences were finally used to determine allelic profiles. Allelic profiles were determined by comparison with 33 Xcc strains obtained from different hosts and regions, whose allelic profiles had been determined previously. A non-redundant database (NRDB) from the pubMLST was used for allelic profile determination and Phyloviz software for constructing a minimum spanning tree. The obtained allelic profile of all Serbian Xcc cabbage strains was 1, 3, 1, 1 for the P-X-dnaK, fyuA, gyrB and rpoD genes, respectively. This profile is assigned as sequence type 2 (ST2) and it coincides with a Portuguese B. oleracea Xcc strain, CPBF 213, originating from B. oleracea var. costata. No connection between sequence type (ST) and the host was detected.
AB  - Xanthomonas campestris pv. campestris (Xcc), prouzrokovač crne truleži kupusa (Brassica oleracea var. capitata L.) svrstava se među najvažnije fitopatogene bakterije koje utiču na pravilno razviće kupusa, dovodeći do gubitka težine glavice i njenog kvaliteta i na taj način drastično smanjuje njegovu ekonomsku vrednost. Ovaj patogen je genetički heterogen, što se ogleda kroz prisustvo dokazanih jedanaest rasa i više od trideset kombinacija alelskih profila širom sveta. Zbog svega navedenog, cilj ovog rada je determinacija alelskih profila Xcc sojeva poreklom sa kupusa prikupljenih 2014. godine. Analiza je vršena kod tri reprezentativna Xcc soja čija je DNK amplifikovana primenom lančane reakcije polimeraze (PCR) sa četiri konzervativna gena - dnaK, fyuA, gyrB i rpoD, a zatim sekvencirana i korišćena za determinaciju alelskih profila. Alelski profili su određivani poređenjem sa 33 Xcc soja izolovana sa različitih domaćina i regiona, čiji su alelski profili prethodno utvrđeni. Nonredundant baza podataka (NRDB) od pubMLST je korišćena za determinaciju alaleskih profila, a Phyloviz softver za konstrukciju Minimum Spanning stabla. Dobijeni alelski profil za sve Xcc sojeve sa kupusa iz Srbije je 1, 3, 1, 1 za gene dnaK, fyuA, gyrB i rpoD, redom. Ovaj profil je označen kao tip sekvence 2 (ST2) i podudara se sa portugalskim B. oleracea Xcc sojem CPBF 213 poreklom sa B. oleracea var. costata. Veza između tipa sekvence (ST) i biljke domaćina nije pronađena.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Allelic profile of Serbian Xanthomonas campestris pv. campestris isolates from cabbage
T1  - Alelski profil izolata Xanthomonas campestris pv. campestris sa kupusa u Srbiji
EP  - 26
IS  - 1
SP  - 19
VL  - 35
DO  - 10.2298/PIF2001019P
ER  - 

@article{
author = "Popović, Tatjana and Jelušić, Aleksandra and Mitrović, Petar and Iličić, Renata and Marković, Sanja",
year = "2020",
abstract = "Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease of cabbage (Brassica oleracea var. capitata L.), is one of the most important bacteria which affect proper cabbage growth, leading to head weight and quality losses and thereby drastically reducing its marketing value. The pathogen is genetically diverse, which is evident from the presence of eleven races worldwide and more than thirty combinations of allelic profiles. Therefore, this study aimed to determine the allelic profiles of Serbian cabbage Xcc strains obtained in 2014. The analysis was done on three selected Xcc strains whose DNA was first amplified using polymerase chain reaction (PCR) with four housekeeping genes - P-XdnaK, fyuA, gyrB, and rpoD, then sequenced, and the obtained sequences were finally used to determine allelic profiles. Allelic profiles were determined by comparison with 33 Xcc strains obtained from different hosts and regions, whose allelic profiles had been determined previously. A non-redundant database (NRDB) from the pubMLST was used for allelic profile determination and Phyloviz software for constructing a minimum spanning tree. The obtained allelic profile of all Serbian Xcc cabbage strains was 1, 3, 1, 1 for the P-X-dnaK, fyuA, gyrB and rpoD genes, respectively. This profile is assigned as sequence type 2 (ST2) and it coincides with a Portuguese B. oleracea Xcc strain, CPBF 213, originating from B. oleracea var. costata. No connection between sequence type (ST) and the host was detected., Xanthomonas campestris pv. campestris (Xcc), prouzrokovač crne truleži kupusa (Brassica oleracea var. capitata L.) svrstava se među najvažnije fitopatogene bakterije koje utiču na pravilno razviće kupusa, dovodeći do gubitka težine glavice i njenog kvaliteta i na taj način drastično smanjuje njegovu ekonomsku vrednost. Ovaj patogen je genetički heterogen, što se ogleda kroz prisustvo dokazanih jedanaest rasa i više od trideset kombinacija alelskih profila širom sveta. Zbog svega navedenog, cilj ovog rada je determinacija alelskih profila Xcc sojeva poreklom sa kupusa prikupljenih 2014. godine. Analiza je vršena kod tri reprezentativna Xcc soja čija je DNK amplifikovana primenom lančane reakcije polimeraze (PCR) sa četiri konzervativna gena - dnaK, fyuA, gyrB i rpoD, a zatim sekvencirana i korišćena za determinaciju alelskih profila. Alelski profili su određivani poređenjem sa 33 Xcc soja izolovana sa različitih domaćina i regiona, čiji su alelski profili prethodno utvrđeni. Nonredundant baza podataka (NRDB) od pubMLST je korišćena za determinaciju alaleskih profila, a Phyloviz softver za konstrukciju Minimum Spanning stabla. Dobijeni alelski profil za sve Xcc sojeve sa kupusa iz Srbije je 1, 3, 1, 1 za gene dnaK, fyuA, gyrB i rpoD, redom. Ovaj profil je označen kao tip sekvence 2 (ST2) i podudara se sa portugalskim B. oleracea Xcc sojem CPBF 213 poreklom sa B. oleracea var. costata. Veza između tipa sekvence (ST) i biljke domaćina nije pronađena.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Allelic profile of Serbian Xanthomonas campestris pv. campestris isolates from cabbage, Alelski profil izolata Xanthomonas campestris pv. campestris sa kupusa u Srbiji",
pages = "26-19",
number = "1",
volume = "35",
doi = "10.2298/PIF2001019P"
}

Popović, T., Jelušić, A., Mitrović, P., Iličić, R.,& Marković, S.. (2020). Allelic profile of Serbian Xanthomonas campestris pv. campestris isolates from cabbage. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 35(1), 19-26.
https://doi.org/10.2298/PIF2001019P

Popović T, Jelušić A, Mitrović P, Iličić R, Marković S. Allelic profile of Serbian Xanthomonas campestris pv. campestris isolates from cabbage. in Pesticidi i fitomedicina. 2020;35(1):19-26.
doi:10.2298/PIF2001019P .

Popović, Tatjana, Jelušić, Aleksandra, Mitrović, Petar, Iličić, Renata, Marković, Sanja, "Allelic profile of Serbian Xanthomonas campestris pv. campestris isolates from cabbage" in Pesticidi i fitomedicina, 35, no. 1 (2020):19-26,
https://doi.org/10.2298/PIF2001019P . .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/3056
AB  - Oilseed rape (Brassica napus L.) is a hybrid species within Brassicaceae family, significant for its oil-rich seed, mainly used for vegetable oil and biodiesel production, but also for consumption as livestock feed. Yield of oilseed rape and other cruciferous crops can be reduced due to different plant pathogenic bacteria, fungi and pests. One of the most significant bacteria affecting crucifers is Xanthomonas campestris pv. campestris (Xcc) causing black rot disease. Xcc isolates from diseased winter oilseed rape plants showing bacterial blight symptom on leaves were collected in nine-year period (2010-2018), from different localities in Serbia, with the aim to examine their genetic features, through sequencing of housekeeping genes. DNA of the obtained isolates was therefore amplified with six primers (dnaK, fyuA, gapA, gyrB, lepA, rpoD) and sent for sequencing. The obtained sequences were checked for homology with strains available in NCBI database.
PB  - Belgrade : Serbian Genetic Society
C3  - Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja
T1  - Genetic diversity of Serbian isolates of Xanthomonas campestris pv. campestris originated from winter oilseed rape
EP  - 163
SP  - 163
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3056
ER  - 

@conference{
author = "Jelušić, Aleksandra and Berić, Tanja and Mitrović, Petar and Marković, Sanja and Stanković, Slaviša and Popović, Tatjana",
year = "2019",
abstract = "Oilseed rape (Brassica napus L.) is a hybrid species within Brassicaceae family, significant for its oil-rich seed, mainly used for vegetable oil and biodiesel production, but also for consumption as livestock feed. Yield of oilseed rape and other cruciferous crops can be reduced due to different plant pathogenic bacteria, fungi and pests. One of the most significant bacteria affecting crucifers is Xanthomonas campestris pv. campestris (Xcc) causing black rot disease. Xcc isolates from diseased winter oilseed rape plants showing bacterial blight symptom on leaves were collected in nine-year period (2010-2018), from different localities in Serbia, with the aim to examine their genetic features, through sequencing of housekeeping genes. DNA of the obtained isolates was therefore amplified with six primers (dnaK, fyuA, gapA, gyrB, lepA, rpoD) and sent for sequencing. The obtained sequences were checked for homology with strains available in NCBI database.",
publisher = "Belgrade : Serbian Genetic Society",
journal = "Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja",
title = "Genetic diversity of Serbian isolates of Xanthomonas campestris pv. campestris originated from winter oilseed rape",
pages = "163-163",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3056"
}

Jelušić, A., Berić, T., Mitrović, P., Marković, S., Stanković, S.,& Popović, T.. (2019). Genetic diversity of Serbian isolates of Xanthomonas campestris pv. campestris originated from winter oilseed rape. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja
Belgrade : Serbian Genetic Society., 163-163.
https://hdl.handle.net/21.15107/rcub_fiver_3056

Jelušić A, Berić T, Mitrović P, Marković S, Stanković S, Popović T. Genetic diversity of Serbian isolates of Xanthomonas campestris pv. campestris originated from winter oilseed rape. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja. 2019;:163-163.
https://hdl.handle.net/21.15107/rcub_fiver_3056 .

Jelušić, Aleksandra, Berić, Tanja, Mitrović, Petar, Marković, Sanja, Stanković, Slaviša, Popović, Tatjana, "Genetic diversity of Serbian isolates of Xanthomonas campestris pv. campestris originated from winter oilseed rape" in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja (2019):163-163,
https://hdl.handle.net/21.15107/rcub_fiver_3056 .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Mitrović, Petar
AU  - Jelušić, Aleksandra
AU  - Dimkić, Ivica
AU  - Marjanović-Jeromela, Ana
AU  - Nikolić, I
AU  - Stanković, S.
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1850
AB  - The present study provides insight into the diversity of 147 Xanthomonas campestris pv. campestris (Xcc) isolates obtained from six Brassica oleracea vegetable crops (broccoli, cabbage, cauliflower, collard greens, kale, kohlrabi) and the winter oilseed rape crop Brassica napus, collected from different regions in Serbia in 2014. The XCF/XCR pathovar-specific primer set was used for fast preliminary identification. In repetitive sequence-based PCR (BOX, ERIC and REP) of all isolates, a higher level of genetic diversity was found in winter oilseed rape isolates compared to isolates from the other hosts. ERIC and REP-PCR showed the highest heterogeneity, with 10 and nine banding patterns, respectively. The REP-PCR results showed the highest correlation (70%) with those obtained with multilocus sequence analysis (MLSA), performed with 10 housekeeping genes (fusA, gap-1, gltA, gyrB1, lacF, lepA, rpoD, dnaK, fyuA and gyrB2). Three distinct phylogenetic groups of winter oilseed rape isolates were detected using MLSA. Two genes, gltA and rpoD, showed the greatest ability to identify and discriminate winter oilseed rape Xcc isolates from isolates of the other six hosts. The lepA gene exhibited specific three-nucleotide changes in sequences of some of the isolates. Results of virulence testing of 18 representative isolates showed statistically significant host-pathogen specialization for Xcc isolates from winter oilseed rape, cauliflower, kale and kohlrabi. In conclusion, oilseed rape isolates are more genetically diverse and show greater specialization to their host in comparison to the rest of the tested isolates from other brassica hosts.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia
EP  - 1457
IS  - 8
SP  - 1448
VL  - 68
DO  - 10.1111/ppa.13064
ER  - 

@article{
author = "Popović, Tatjana and Mitrović, Petar and Jelušić, Aleksandra and Dimkić, Ivica and Marjanović-Jeromela, Ana and Nikolić, I and Stanković, S.",
year = "2019",
abstract = "The present study provides insight into the diversity of 147 Xanthomonas campestris pv. campestris (Xcc) isolates obtained from six Brassica oleracea vegetable crops (broccoli, cabbage, cauliflower, collard greens, kale, kohlrabi) and the winter oilseed rape crop Brassica napus, collected from different regions in Serbia in 2014. The XCF/XCR pathovar-specific primer set was used for fast preliminary identification. In repetitive sequence-based PCR (BOX, ERIC and REP) of all isolates, a higher level of genetic diversity was found in winter oilseed rape isolates compared to isolates from the other hosts. ERIC and REP-PCR showed the highest heterogeneity, with 10 and nine banding patterns, respectively. The REP-PCR results showed the highest correlation (70%) with those obtained with multilocus sequence analysis (MLSA), performed with 10 housekeeping genes (fusA, gap-1, gltA, gyrB1, lacF, lepA, rpoD, dnaK, fyuA and gyrB2). Three distinct phylogenetic groups of winter oilseed rape isolates were detected using MLSA. Two genes, gltA and rpoD, showed the greatest ability to identify and discriminate winter oilseed rape Xcc isolates from isolates of the other six hosts. The lepA gene exhibited specific three-nucleotide changes in sequences of some of the isolates. Results of virulence testing of 18 representative isolates showed statistically significant host-pathogen specialization for Xcc isolates from winter oilseed rape, cauliflower, kale and kohlrabi. In conclusion, oilseed rape isolates are more genetically diverse and show greater specialization to their host in comparison to the rest of the tested isolates from other brassica hosts.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia",
pages = "1457-1448",
number = "8",
volume = "68",
doi = "10.1111/ppa.13064"
}

Popović, T., Mitrović, P., Jelušić, A., Dimkić, I., Marjanović-Jeromela, A., Nikolić, I.,& Stanković, S.. (2019). Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia. in Plant Pathology
Wiley, Hoboken., 68(8), 1448-1457.
https://doi.org/10.1111/ppa.13064

Popović T, Mitrović P, Jelušić A, Dimkić I, Marjanović-Jeromela A, Nikolić I, Stanković S. Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia. in Plant Pathology. 2019;68(8):1448-1457.
doi:10.1111/ppa.13064 .

Popović, Tatjana, Mitrović, Petar, Jelušić, Aleksandra, Dimkić, Ivica, Marjanović-Jeromela, Ana, Nikolić, I, Stanković, S., "Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia" in Plant Pathology, 68, no. 8 (2019):1448-1457,
https://doi.org/10.1111/ppa.13064 . .