TY  - JOUR
AU  - Boivin, Stephane
AU  - Lahmidi, Nassima Ait
AU  - Sherlock, David
AU  - Bonhomme, Maxime
AU  - Dijon, Doriane
AU  - Heulin-Gotty, Karine
AU  - Le-Quere, Antoine
AU  - Pervent, Marjorie
AU  - Tauzin, Marc
AU  - Carlsson, Georg
AU  - Jensen, Erik
AU  - Journet, Etienne-Pascal
AU  - Lopez-Bellido, Raphael
AU  - Seidenglanz, Marek
AU  - Marinković, Jelena
AU  - Colella, Stefano
AU  - Brunel, Brigitte
AU  - Young, Peter
AU  - Lepetit, Marc
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/1993
AB  - Fabeae legumes such as pea and faba bean form symbiotic nodules with a large diversity of soil Rhizobium leguminosarum symbiovar viciae (Rlv) bacteria. However, bacteria competitive to form root nodules (CFN) are generally not the most efficient to fix dinitrogen, resulting in a decrease in legume crop yields. Here, we investigate differential selection by host plants on the diversity of Rlv. A large collection of Rlv was collected by nodule trapping with pea and faba bean from soils at five European sites. Representative genomes were sequenced. In parallel, diversity and abundance of Rlv were estimated directly in these soils using metabarcoding. The CFN of isolates was measured with both legume hosts. Pea/faba bean CFN were associated to Rlv genomic regions. Variations of bacterial pea and/or faba bean CFN explained the differential abundance of Rlv genotypes in pea and faba bean nodules. No evidence was found for genetic association between CFN and variations in the core genome, but variations in specific regions of the nod locus, as well as in other plasmid loci, were associated with differences in CFN. These findings shed light on the genetic control of CFN in Rlv and emphasise the importance of host plants in controlling Rhizobium diversity.
PB  - Wiley, Hoboken
T2  - New Phytologist
T1  - Host-specific competitiveness to form nodules in Rhizobium leguminosarum symbiovar viciae
EP  - 568
IS  - 2
SP  - 555
VL  - 226
DO  - 10.1111/nph.16392
ER  - 

@article{
author = "Boivin, Stephane and Lahmidi, Nassima Ait and Sherlock, David and Bonhomme, Maxime and Dijon, Doriane and Heulin-Gotty, Karine and Le-Quere, Antoine and Pervent, Marjorie and Tauzin, Marc and Carlsson, Georg and Jensen, Erik and Journet, Etienne-Pascal and Lopez-Bellido, Raphael and Seidenglanz, Marek and Marinković, Jelena and Colella, Stefano and Brunel, Brigitte and Young, Peter and Lepetit, Marc",
year = "2020",
abstract = "Fabeae legumes such as pea and faba bean form symbiotic nodules with a large diversity of soil Rhizobium leguminosarum symbiovar viciae (Rlv) bacteria. However, bacteria competitive to form root nodules (CFN) are generally not the most efficient to fix dinitrogen, resulting in a decrease in legume crop yields. Here, we investigate differential selection by host plants on the diversity of Rlv. A large collection of Rlv was collected by nodule trapping with pea and faba bean from soils at five European sites. Representative genomes were sequenced. In parallel, diversity and abundance of Rlv were estimated directly in these soils using metabarcoding. The CFN of isolates was measured with both legume hosts. Pea/faba bean CFN were associated to Rlv genomic regions. Variations of bacterial pea and/or faba bean CFN explained the differential abundance of Rlv genotypes in pea and faba bean nodules. No evidence was found for genetic association between CFN and variations in the core genome, but variations in specific regions of the nod locus, as well as in other plasmid loci, were associated with differences in CFN. These findings shed light on the genetic control of CFN in Rlv and emphasise the importance of host plants in controlling Rhizobium diversity.",
publisher = "Wiley, Hoboken",
journal = "New Phytologist",
title = "Host-specific competitiveness to form nodules in Rhizobium leguminosarum symbiovar viciae",
pages = "568-555",
number = "2",
volume = "226",
doi = "10.1111/nph.16392"
}

Boivin, S., Lahmidi, N. A., Sherlock, D., Bonhomme, M., Dijon, D., Heulin-Gotty, K., Le-Quere, A., Pervent, M., Tauzin, M., Carlsson, G., Jensen, E., Journet, E., Lopez-Bellido, R., Seidenglanz, M., Marinković, J., Colella, S., Brunel, B., Young, P.,& Lepetit, M.. (2020). Host-specific competitiveness to form nodules in Rhizobium leguminosarum symbiovar viciae. in New Phytologist
Wiley, Hoboken., 226(2), 555-568.
https://doi.org/10.1111/nph.16392

Boivin S, Lahmidi NA, Sherlock D, Bonhomme M, Dijon D, Heulin-Gotty K, Le-Quere A, Pervent M, Tauzin M, Carlsson G, Jensen E, Journet E, Lopez-Bellido R, Seidenglanz M, Marinković J, Colella S, Brunel B, Young P, Lepetit M. Host-specific competitiveness to form nodules in Rhizobium leguminosarum symbiovar viciae. in New Phytologist. 2020;226(2):555-568.
doi:10.1111/nph.16392 .

Boivin, Stephane, Lahmidi, Nassima Ait, Sherlock, David, Bonhomme, Maxime, Dijon, Doriane, Heulin-Gotty, Karine, Le-Quere, Antoine, Pervent, Marjorie, Tauzin, Marc, Carlsson, Georg, Jensen, Erik, Journet, Etienne-Pascal, Lopez-Bellido, Raphael, Seidenglanz, Marek, Marinković, Jelena, Colella, Stefano, Brunel, Brigitte, Young, Peter, Lepetit, Marc, "Host-specific competitiveness to form nodules in Rhizobium leguminosarum symbiovar viciae" in New Phytologist, 226, no. 2 (2020):555-568,
https://doi.org/10.1111/nph.16392 . .

TY  - CONF
AU  - Ait Lahmidi, Nassima
AU  - Sherlock, David
AU  - Dijon, Doriane
AU  - Heulin-Gotty, Karine
AU  - Pervent, Marjorie
AU  - Le-Quere, Antoine
AU  - Seidenglanz, Marek
AU  - Lopez-Bellido, Raphael
AU  - Marinković, Jelena
AU  - Carlsson, Georg
AU  - Jensen, Erik
AU  - Journet, Etienne-Pascal
AU  - Brunel, Brigitte
AU  - Lepetit, Marc
AU  - Young, Peter
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/2772
AB  - Soil is an heterogeneous and fluctuating environment. Water limitation in agronomic soils is often partial but lead locally to the suppression of symbiotic activity and therefore to reduction of N acquisition by the plant. A split-root system was developed in pea to evaluate the capacities of Pea-Rhizobium leguminosarum bv viciae (Rlv) symbiotic associations to compensate a local reduction of water availability. Root systems are split in two parts watered separately. Soil water content was monitored. Water limitation was exerted by stopping watering of a half root system. Arrest of nitrogen fixation is very rapidly observed in root directly exposed to water stress. This local and partial water limitation of the plant triggered a systemic signaling on well-watered roots of the same plant. The compensatory response occurs by increasing nodule biomass (and therefore nitrogen fixation capacities) of these roots not directly exposed to the water limitation. In preliminary experiments pea plants were inoculated with a reference Rlv strain. Response of and root and nodule development that compensate the local water limitation was confirmed. Interestingly, the compensatory response varies according to the Rlv strain as another Rlv strain, displaying similar nitrogen fixation efficiency in watered conditions, was not able to promote equivalent compensatory response. This experimental system has been used as a screen to select Rlv strains able to promote rapid compensatory responses to water stress.
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, International Conference „Advances in Grain Legume Breeding, Cultivation and Uses for a More Competitive Value-chain“, 27-28 September 2017, Novi Sad
T1  - Inoculation requirement of pea and faba bean and selection of Rlv strains
EP  - 117
SP  - 117
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2772
ER  - 

@conference{
author = "Ait Lahmidi, Nassima and Sherlock, David and Dijon, Doriane and Heulin-Gotty, Karine and Pervent, Marjorie and Le-Quere, Antoine and Seidenglanz, Marek and Lopez-Bellido, Raphael and Marinković, Jelena and Carlsson, Georg and Jensen, Erik and Journet, Etienne-Pascal and Brunel, Brigitte and Lepetit, Marc and Young, Peter",
year = "2017",
abstract = "Soil is an heterogeneous and fluctuating environment. Water limitation in agronomic soils is often partial but lead locally to the suppression of symbiotic activity and therefore to reduction of N acquisition by the plant. A split-root system was developed in pea to evaluate the capacities of Pea-Rhizobium leguminosarum bv viciae (Rlv) symbiotic associations to compensate a local reduction of water availability. Root systems are split in two parts watered separately. Soil water content was monitored. Water limitation was exerted by stopping watering of a half root system. Arrest of nitrogen fixation is very rapidly observed in root directly exposed to water stress. This local and partial water limitation of the plant triggered a systemic signaling on well-watered roots of the same plant. The compensatory response occurs by increasing nodule biomass (and therefore nitrogen fixation capacities) of these roots not directly exposed to the water limitation. In preliminary experiments pea plants were inoculated with a reference Rlv strain. Response of and root and nodule development that compensate the local water limitation was confirmed. Interestingly, the compensatory response varies according to the Rlv strain as another Rlv strain, displaying similar nitrogen fixation efficiency in watered conditions, was not able to promote equivalent compensatory response. This experimental system has been used as a screen to select Rlv strains able to promote rapid compensatory responses to water stress.",
publisher = "Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, International Conference „Advances in Grain Legume Breeding, Cultivation and Uses for a More Competitive Value-chain“, 27-28 September 2017, Novi Sad",
title = "Inoculation requirement of pea and faba bean and selection of Rlv strains",
pages = "117-117",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2772"
}

Ait Lahmidi, N., Sherlock, D., Dijon, D., Heulin-Gotty, K., Pervent, M., Le-Quere, A., Seidenglanz, M., Lopez-Bellido, R., Marinković, J., Carlsson, G., Jensen, E., Journet, E., Brunel, B., Lepetit, M.,& Young, P.. (2017). Inoculation requirement of pea and faba bean and selection of Rlv strains. in Book of Abstracts, International Conference „Advances in Grain Legume Breeding, Cultivation and Uses for a More Competitive Value-chain“, 27-28 September 2017, Novi Sad
Novi Sad : Institute of Field and Vegetable Crops., 117-117.
https://hdl.handle.net/21.15107/rcub_fiver_2772

Ait Lahmidi N, Sherlock D, Dijon D, Heulin-Gotty K, Pervent M, Le-Quere A, Seidenglanz M, Lopez-Bellido R, Marinković J, Carlsson G, Jensen E, Journet E, Brunel B, Lepetit M, Young P. Inoculation requirement of pea and faba bean and selection of Rlv strains. in Book of Abstracts, International Conference „Advances in Grain Legume Breeding, Cultivation and Uses for a More Competitive Value-chain“, 27-28 September 2017, Novi Sad. 2017;:117-117.
https://hdl.handle.net/21.15107/rcub_fiver_2772 .

Ait Lahmidi, Nassima, Sherlock, David, Dijon, Doriane, Heulin-Gotty, Karine, Pervent, Marjorie, Le-Quere, Antoine, Seidenglanz, Marek, Lopez-Bellido, Raphael, Marinković, Jelena, Carlsson, Georg, Jensen, Erik, Journet, Etienne-Pascal, Brunel, Brigitte, Lepetit, Marc, Young, Peter, "Inoculation requirement of pea and faba bean and selection of Rlv strains" in Book of Abstracts, International Conference „Advances in Grain Legume Breeding, Cultivation and Uses for a More Competitive Value-chain“, 27-28 September 2017, Novi Sad (2017):117-117,
https://hdl.handle.net/21.15107/rcub_fiver_2772 .